Amino acid dipepetide frequency for Moraxella phage Mcat27

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.69AlaAla: 3.69 ± 0.615
1.476AlaCys: 1.476 ± 0.351
5.248AlaAsp: 5.248 ± 0.607
5.002AlaGlu: 5.002 ± 0.595
2.952AlaPhe: 2.952 ± 0.53
5.576AlaGly: 5.576 ± 0.778
2.132AlaHis: 2.132 ± 0.392
5.822AlaIle: 5.822 ± 0.643
6.56AlaLys: 6.56 ± 1.229
7.462AlaLeu: 7.462 ± 0.885
2.132AlaMet: 2.132 ± 0.451
4.346AlaAsn: 4.346 ± 0.971
2.05AlaPro: 2.05 ± 0.478
3.608AlaGln: 3.608 ± 0.614
3.526AlaArg: 3.526 ± 0.553
6.478AlaSer: 6.478 ± 0.908
5.084AlaThr: 5.084 ± 0.917
5.494AlaVal: 5.494 ± 0.75
1.312AlaTrp: 1.312 ± 0.287
3.198AlaTyr: 3.198 ± 0.515
0.0AlaXaa: 0.0 ± 0.0
Cys
0.738CysAla: 0.738 ± 0.262
0.164CysCys: 0.164 ± 0.103
0.41CysAsp: 0.41 ± 0.173
0.656CysGlu: 0.656 ± 0.288
0.328CysPhe: 0.328 ± 0.151
0.902CysGly: 0.902 ± 0.334
0.492CysHis: 0.492 ± 0.182
0.328CysIle: 0.328 ± 0.153
0.738CysLys: 0.738 ± 0.246
0.738CysLeu: 0.738 ± 0.252
0.164CysMet: 0.164 ± 0.12
0.328CysAsn: 0.328 ± 0.18
0.574CysPro: 0.574 ± 0.271
0.574CysGln: 0.574 ± 0.176
0.902CysArg: 0.902 ± 0.289
0.82CysSer: 0.82 ± 0.218
0.41CysThr: 0.41 ± 0.236
0.82CysVal: 0.82 ± 0.258
0.082CysTrp: 0.082 ± 0.089
0.574CysTyr: 0.574 ± 0.258
0.0CysXaa: 0.0 ± 0.0
Asp
4.51AspAla: 4.51 ± 0.587
0.574AspCys: 0.574 ± 0.225
5.494AspAsp: 5.494 ± 0.7
5.002AspGlu: 5.002 ± 0.53
3.444AspPhe: 3.444 ± 0.629
6.56AspGly: 6.56 ± 0.671
0.738AspHis: 0.738 ± 0.252
4.51AspIle: 4.51 ± 0.66
4.346AspLys: 4.346 ± 0.513
4.838AspLeu: 4.838 ± 0.541
1.558AspMet: 1.558 ± 0.258
3.28AspAsn: 3.28 ± 0.434
1.722AspPro: 1.722 ± 0.396
1.23AspGln: 1.23 ± 0.309
2.378AspArg: 2.378 ± 0.392
3.69AspSer: 3.69 ± 0.461
2.952AspThr: 2.952 ± 0.449
3.772AspVal: 3.772 ± 0.546
0.82AspTrp: 0.82 ± 0.21
2.378AspTyr: 2.378 ± 0.461
0.0AspXaa: 0.0 ± 0.0
Glu
3.69GluAla: 3.69 ± 0.498
0.82GluCys: 0.82 ± 0.246
2.706GluAsp: 2.706 ± 0.605
1.886GluGlu: 1.886 ± 0.394
3.034GluPhe: 3.034 ± 0.623
2.46GluGly: 2.46 ± 0.349
1.722GluHis: 1.722 ± 0.453
4.838GluIle: 4.838 ± 0.61
4.1GluLys: 4.1 ± 0.605
7.38GluLeu: 7.38 ± 0.624
2.624GluMet: 2.624 ± 0.36
2.624GluAsn: 2.624 ± 0.573
2.214GluPro: 2.214 ± 0.48
4.018GluGln: 4.018 ± 0.609
3.608GluArg: 3.608 ± 0.48
2.214GluSer: 2.214 ± 0.505
2.788GluThr: 2.788 ± 0.471
3.444GluVal: 3.444 ± 0.602
0.738GluTrp: 0.738 ± 0.216
2.296GluTyr: 2.296 ± 0.356
0.0GluXaa: 0.0 ± 0.0
Phe
2.378PheAla: 2.378 ± 0.51
0.82PheCys: 0.82 ± 0.236
3.28PheAsp: 3.28 ± 0.515
3.198PheGlu: 3.198 ± 0.498
0.984PhePhe: 0.984 ± 0.292
3.28PheGly: 3.28 ± 0.543
0.82PheHis: 0.82 ± 0.223
2.542PheIle: 2.542 ± 0.457
1.312PheLys: 1.312 ± 0.315
2.624PheLeu: 2.624 ± 0.584
1.066PheMet: 1.066 ± 0.286
1.64PheAsn: 1.64 ± 0.358
1.066PhePro: 1.066 ± 0.238
0.574PheGln: 0.574 ± 0.228
1.394PheArg: 1.394 ± 0.357
2.46PheSer: 2.46 ± 0.438
2.132PheThr: 2.132 ± 0.351
2.378PheVal: 2.378 ± 0.593
0.574PheTrp: 0.574 ± 0.186
1.312PheTyr: 1.312 ± 0.329
0.0PheXaa: 0.0 ± 0.0
Gly
3.69GlyAla: 3.69 ± 0.617
0.574GlyCys: 0.574 ± 0.176
3.362GlyAsp: 3.362 ± 0.599
4.838GlyGlu: 4.838 ± 0.728
3.198GlyPhe: 3.198 ± 0.504
4.592GlyGly: 4.592 ± 1.054
1.312GlyHis: 1.312 ± 0.389
3.69GlyIle: 3.69 ± 0.373
6.15GlyLys: 6.15 ± 0.617
6.314GlyLeu: 6.314 ± 0.576
2.132GlyMet: 2.132 ± 0.346
3.116GlyAsn: 3.116 ± 0.552
0.246GlyPro: 0.246 ± 0.167
2.132GlyGln: 2.132 ± 0.54
3.69GlyArg: 3.69 ± 0.456
4.1GlySer: 4.1 ± 0.581
2.788GlyThr: 2.788 ± 0.524
5.33GlyVal: 5.33 ± 0.669
1.066GlyTrp: 1.066 ± 0.322
2.05GlyTyr: 2.05 ± 0.571
0.0GlyXaa: 0.0 ± 0.0
His
2.46HisAla: 2.46 ± 0.465
0.246HisCys: 0.246 ± 0.132
1.968HisAsp: 1.968 ± 0.355
1.476HisGlu: 1.476 ± 0.395
0.82HisPhe: 0.82 ± 0.212
1.968HisGly: 1.968 ± 0.46
1.394HisHis: 1.394 ± 0.417
1.312HisIle: 1.312 ± 0.314
1.23HisLys: 1.23 ± 0.25
2.706HisLeu: 2.706 ± 0.396
0.328HisMet: 0.328 ± 0.215
0.902HisAsn: 0.902 ± 0.298
0.984HisPro: 0.984 ± 0.265
0.984HisGln: 0.984 ± 0.242
1.066HisArg: 1.066 ± 0.297
1.476HisSer: 1.476 ± 0.349
2.05HisThr: 2.05 ± 0.449
0.738HisVal: 0.738 ± 0.206
0.41HisTrp: 0.41 ± 0.204
0.984HisTyr: 0.984 ± 0.298
0.0HisXaa: 0.0 ± 0.0
Ile
5.576IleAla: 5.576 ± 0.724
0.902IleCys: 0.902 ± 0.283
5.002IleAsp: 5.002 ± 0.637
4.182IleGlu: 4.182 ± 0.504
1.804IlePhe: 1.804 ± 0.372
4.346IleGly: 4.346 ± 0.569
1.394IleHis: 1.394 ± 0.339
4.264IleIle: 4.264 ± 0.679
5.904IleLys: 5.904 ± 0.727
4.592IleLeu: 4.592 ± 0.459
1.722IleMet: 1.722 ± 0.392
3.608IleAsn: 3.608 ± 0.518
1.968IlePro: 1.968 ± 0.422
2.378IleGln: 2.378 ± 0.472
2.132IleArg: 2.132 ± 0.373
4.018IleSer: 4.018 ± 0.812
5.33IleThr: 5.33 ± 0.851
2.46IleVal: 2.46 ± 0.419
0.246IleTrp: 0.246 ± 0.135
2.624IleTyr: 2.624 ± 0.483
0.0IleXaa: 0.0 ± 0.0
Lys
6.97LysAla: 6.97 ± 0.754
0.492LysCys: 0.492 ± 0.217
3.69LysAsp: 3.69 ± 0.628
2.788LysGlu: 2.788 ± 0.47
1.968LysPhe: 1.968 ± 0.43
3.608LysGly: 3.608 ± 0.582
0.984LysHis: 0.984 ± 0.294
4.838LysIle: 4.838 ± 0.596
4.674LysLys: 4.674 ± 0.833
5.904LysLeu: 5.904 ± 0.589
1.968LysMet: 1.968 ± 0.519
3.936LysAsn: 3.936 ± 0.677
3.116LysPro: 3.116 ± 0.578
3.936LysGln: 3.936 ± 0.644
3.28LysArg: 3.28 ± 0.508
5.412LysSer: 5.412 ± 0.694
4.428LysThr: 4.428 ± 0.639
3.198LysVal: 3.198 ± 0.516
0.984LysTrp: 0.984 ± 0.282
1.804LysTyr: 1.804 ± 0.376
0.0LysXaa: 0.0 ± 0.0
Leu
9.594LeuAla: 9.594 ± 1.223
0.902LeuCys: 0.902 ± 0.317
5.822LeuAsp: 5.822 ± 0.612
5.658LeuGlu: 5.658 ± 0.577
2.46LeuPhe: 2.46 ± 0.433
6.068LeuGly: 6.068 ± 1.022
2.378LeuHis: 2.378 ± 0.421
5.084LeuIle: 5.084 ± 0.686
6.068LeuLys: 6.068 ± 0.681
6.888LeuLeu: 6.888 ± 0.936
1.558LeuMet: 1.558 ± 0.417
3.608LeuAsn: 3.608 ± 0.528
3.608LeuPro: 3.608 ± 0.604
4.182LeuGln: 4.182 ± 0.425
4.018LeuArg: 4.018 ± 0.552
6.314LeuSer: 6.314 ± 0.655
5.33LeuThr: 5.33 ± 0.68
4.264LeuVal: 4.264 ± 0.585
1.066LeuTrp: 1.066 ± 0.233
3.28LeuTyr: 3.28 ± 0.51
0.0LeuXaa: 0.0 ± 0.0
Met
2.46MetAla: 2.46 ± 0.599
0.164MetCys: 0.164 ± 0.118
1.394MetAsp: 1.394 ± 0.276
1.066MetGlu: 1.066 ± 0.275
0.902MetPhe: 0.902 ± 0.265
1.64MetGly: 1.64 ± 0.32
0.738MetHis: 0.738 ± 0.248
1.886MetIle: 1.886 ± 0.599
1.312MetLys: 1.312 ± 0.326
2.132MetLeu: 2.132 ± 0.553
1.066MetMet: 1.066 ± 0.273
1.722MetAsn: 1.722 ± 0.484
1.23MetPro: 1.23 ± 0.317
1.722MetGln: 1.722 ± 0.443
1.23MetArg: 1.23 ± 0.29
2.132MetSer: 2.132 ± 0.457
1.804MetThr: 1.804 ± 0.345
2.05MetVal: 2.05 ± 0.601
0.082MetTrp: 0.082 ± 0.09
0.574MetTyr: 0.574 ± 0.229
0.0MetXaa: 0.0 ± 0.0
Asn
4.674AsnAla: 4.674 ± 0.765
0.41AsnCys: 0.41 ± 0.204
2.952AsnAsp: 2.952 ± 0.507
2.46AsnGlu: 2.46 ± 0.355
2.214AsnPhe: 2.214 ± 0.47
2.87AsnGly: 2.87 ± 0.426
1.804AsnHis: 1.804 ± 0.459
2.624AsnIle: 2.624 ± 0.383
3.198AsnLys: 3.198 ± 0.734
4.674AsnLeu: 4.674 ± 0.668
1.312AsnMet: 1.312 ± 0.297
2.788AsnAsn: 2.788 ± 0.565
2.214AsnPro: 2.214 ± 0.407
2.296AsnGln: 2.296 ± 0.411
2.542AsnArg: 2.542 ± 0.545
3.69AsnSer: 3.69 ± 0.803
2.706AsnThr: 2.706 ± 0.401
2.542AsnVal: 2.542 ± 0.412
0.246AsnTrp: 0.246 ± 0.139
1.804AsnTyr: 1.804 ± 0.444
0.0AsnXaa: 0.0 ± 0.0
Pro
2.542ProAla: 2.542 ± 0.452
0.574ProCys: 0.574 ± 0.18
2.05ProAsp: 2.05 ± 0.458
1.64ProGlu: 1.64 ± 0.408
1.558ProPhe: 1.558 ± 0.33
0.492ProGly: 0.492 ± 0.195
0.738ProHis: 0.738 ± 0.225
3.116ProIle: 3.116 ± 0.378
1.968ProLys: 1.968 ± 0.463
2.132ProLeu: 2.132 ± 0.468
0.82ProMet: 0.82 ± 0.23
2.46ProAsn: 2.46 ± 0.539
1.23ProPro: 1.23 ± 0.326
1.312ProGln: 1.312 ± 0.363
1.23ProArg: 1.23 ± 0.356
3.28ProSer: 3.28 ± 0.624
2.542ProThr: 2.542 ± 0.423
2.214ProVal: 2.214 ± 0.474
0.41ProTrp: 0.41 ± 0.176
0.574ProTyr: 0.574 ± 0.236
0.0ProXaa: 0.0 ± 0.0
Gln
5.084GlnAla: 5.084 ± 0.644
0.328GlnCys: 0.328 ± 0.179
2.624GlnAsp: 2.624 ± 0.419
2.542GlnGlu: 2.542 ± 0.546
1.066GlnPhe: 1.066 ± 0.264
2.378GlnGly: 2.378 ± 0.418
0.574GlnHis: 0.574 ± 0.202
3.116GlnIle: 3.116 ± 0.512
3.69GlnLys: 3.69 ± 0.458
3.526GlnLeu: 3.526 ± 0.494
1.558GlnMet: 1.558 ± 0.31
1.804GlnAsn: 1.804 ± 0.402
1.148GlnPro: 1.148 ± 0.302
1.722GlnGln: 1.722 ± 0.482
2.378GlnArg: 2.378 ± 0.543
2.952GlnSer: 2.952 ± 0.438
3.444GlnThr: 3.444 ± 0.481
2.624GlnVal: 2.624 ± 0.483
0.656GlnTrp: 0.656 ± 0.24
2.05GlnTyr: 2.05 ± 0.385
0.0GlnXaa: 0.0 ± 0.0
Arg
4.756ArgAla: 4.756 ± 0.524
0.41ArgCys: 0.41 ± 0.151
2.624ArgAsp: 2.624 ± 0.318
3.28ArgGlu: 3.28 ± 0.634
2.214ArgPhe: 2.214 ± 0.555
1.968ArgGly: 1.968 ± 0.412
1.558ArgHis: 1.558 ± 0.268
2.87ArgIle: 2.87 ± 0.456
2.378ArgLys: 2.378 ± 0.453
5.248ArgLeu: 5.248 ± 0.813
1.066ArgMet: 1.066 ± 0.307
1.722ArgAsn: 1.722 ± 0.356
1.148ArgPro: 1.148 ± 0.27
2.706ArgGln: 2.706 ± 0.396
1.886ArgArg: 1.886 ± 0.418
3.116ArgSer: 3.116 ± 0.501
3.362ArgThr: 3.362 ± 0.424
2.296ArgVal: 2.296 ± 0.396
0.82ArgTrp: 0.82 ± 0.226
2.378ArgTyr: 2.378 ± 0.444
0.0ArgXaa: 0.0 ± 0.0
Ser
5.084SerAla: 5.084 ± 0.779
0.328SerCys: 0.328 ± 0.151
3.936SerAsp: 3.936 ± 0.658
4.674SerGlu: 4.674 ± 0.502
2.542SerPhe: 2.542 ± 0.509
3.608SerGly: 3.608 ± 0.573
2.542SerHis: 2.542 ± 0.656
3.854SerIle: 3.854 ± 0.625
4.264SerLys: 4.264 ± 0.532
6.806SerLeu: 6.806 ± 0.851
0.984SerMet: 0.984 ± 0.256
4.51SerAsn: 4.51 ± 0.814
1.722SerPro: 1.722 ± 0.437
2.706SerGln: 2.706 ± 0.69
3.772SerArg: 3.772 ± 0.474
3.854SerSer: 3.854 ± 1.027
3.28SerThr: 3.28 ± 0.485
5.166SerVal: 5.166 ± 0.711
0.902SerTrp: 0.902 ± 0.32
1.64SerTyr: 1.64 ± 0.403
0.0SerXaa: 0.0 ± 0.0
Thr
7.38ThrAla: 7.38 ± 1.275
0.246ThrCys: 0.246 ± 0.128
4.1ThrAsp: 4.1 ± 0.549
3.608ThrGlu: 3.608 ± 0.62
1.312ThrPhe: 1.312 ± 0.289
4.018ThrGly: 4.018 ± 0.586
1.476ThrHis: 1.476 ± 0.243
3.116ThrIle: 3.116 ± 0.497
3.936ThrLys: 3.936 ± 0.723
5.33ThrLeu: 5.33 ± 0.692
1.886ThrMet: 1.886 ± 0.355
3.772ThrAsn: 3.772 ± 0.69
3.28ThrPro: 3.28 ± 0.43
2.624ThrGln: 2.624 ± 0.381
1.804ThrArg: 1.804 ± 0.358
3.034ThrSer: 3.034 ± 0.628
3.28ThrThr: 3.28 ± 0.596
3.772ThrVal: 3.772 ± 0.381
0.902ThrTrp: 0.902 ± 0.247
1.312ThrTyr: 1.312 ± 0.23
0.0ThrXaa: 0.0 ± 0.0
Val
4.428ValAla: 4.428 ± 0.661
0.738ValCys: 0.738 ± 0.236
3.608ValAsp: 3.608 ± 0.539
2.706ValGlu: 2.706 ± 0.41
1.558ValPhe: 1.558 ± 0.331
4.182ValGly: 4.182 ± 0.603
1.23ValHis: 1.23 ± 0.315
3.69ValIle: 3.69 ± 0.543
3.772ValLys: 3.772 ± 0.548
4.756ValLeu: 4.756 ± 0.736
1.968ValMet: 1.968 ± 0.355
2.46ValAsn: 2.46 ± 0.383
1.64ValPro: 1.64 ± 0.291
3.362ValGln: 3.362 ± 0.387
3.526ValArg: 3.526 ± 0.555
4.018ValSer: 4.018 ± 0.479
4.346ValThr: 4.346 ± 0.593
3.526ValVal: 3.526 ± 0.582
1.23ValTrp: 1.23 ± 0.249
2.296ValTyr: 2.296 ± 0.408
0.0ValXaa: 0.0 ± 0.0
Trp
0.902TrpAla: 0.902 ± 0.23
0.164TrpCys: 0.164 ± 0.114
1.148TrpAsp: 1.148 ± 0.272
0.738TrpGlu: 0.738 ± 0.228
0.41TrpPhe: 0.41 ± 0.178
0.492TrpGly: 0.492 ± 0.161
0.164TrpHis: 0.164 ± 0.119
0.492TrpIle: 0.492 ± 0.189
0.492TrpLys: 0.492 ± 0.198
1.148TrpLeu: 1.148 ± 0.343
0.328TrpMet: 0.328 ± 0.173
0.328TrpAsn: 0.328 ± 0.225
0.328TrpPro: 0.328 ± 0.14
1.148TrpGln: 1.148 ± 0.292
0.984TrpArg: 0.984 ± 0.313
1.148TrpSer: 1.148 ± 0.316
0.492TrpThr: 0.492 ± 0.17
1.066TrpVal: 1.066 ± 0.29
0.246TrpTrp: 0.246 ± 0.124
0.82TrpTyr: 0.82 ± 0.229
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.542TyrAla: 2.542 ± 0.505
0.41TyrCys: 0.41 ± 0.169
2.378TyrAsp: 2.378 ± 0.478
1.804TyrGlu: 1.804 ± 0.458
1.066TyrPhe: 1.066 ± 0.31
3.362TyrGly: 3.362 ± 0.545
1.394TyrHis: 1.394 ± 0.311
2.46TyrIle: 2.46 ± 0.468
1.886TyrLys: 1.886 ± 0.441
2.952TyrLeu: 2.952 ± 0.557
0.984TyrMet: 0.984 ± 0.273
1.066TyrAsn: 1.066 ± 0.351
1.394TyrPro: 1.394 ± 0.336
2.05TyrGln: 2.05 ± 0.393
2.46TyrArg: 2.46 ± 0.441
1.968TyrSer: 1.968 ± 0.446
1.64TyrThr: 1.64 ± 0.309
1.886TyrVal: 1.886 ± 0.406
0.246TyrTrp: 0.246 ± 0.144
1.148TyrTyr: 1.148 ± 0.306
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12196 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski