Kribbella sp. VKM Ac-2575

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Kribbellaceae; Kribbella; unclassified Kribbella

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7379 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R7TJB8|A0A4R7TJB8_9ACTN Pimeloyl-ACP methyl ester carboxylesterase OS=Kribbella sp. VKM Ac-2575 OX=2512212 GN=EV138_5409 PE=4 SV=1
MM1 pKa = 7.7SDD3 pKa = 4.74DD4 pKa = 3.49IRR6 pKa = 11.84LSGTTVNAPDD16 pKa = 4.07ALALATFYY24 pKa = 11.64AEE26 pKa = 4.21LTGGEE31 pKa = 4.29ALGSEE36 pKa = 4.46HH37 pKa = 7.26WATVSGPNGFIAFQQVDD54 pKa = 4.38DD55 pKa = 4.73FCPPVWPGADD65 pKa = 3.09VPMQMHH71 pKa = 7.35LDD73 pKa = 4.06FFVDD77 pKa = 3.76DD78 pKa = 5.06LEE80 pKa = 4.59ATGARR85 pKa = 11.84AVAAGATRR93 pKa = 11.84LDD95 pKa = 3.67FQPNSDD101 pKa = 3.62HH102 pKa = 6.62CFVYY106 pKa = 10.62ADD108 pKa = 3.81PAGHH112 pKa = 7.09PFCLSTWDD120 pKa = 4.26GPHH123 pKa = 7.08LNDD126 pKa = 5.92DD127 pKa = 3.93PDD129 pKa = 3.5EE130 pKa = 4.31

Molecular weight: 13.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R7TDH6|A0A4R7TDH6_9ACTN PhoD-like phosphatase OS=Kribbella sp. VKM Ac-2575 OX=2512212 GN=EV138_3117 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 10.43GRR40 pKa = 11.84QRR42 pKa = 11.84LAAA45 pKa = 4.56

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7379

0

7379

2437599

29

3831

330.3

35.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.781 ± 0.036

0.695 ± 0.009

5.999 ± 0.025

5.333 ± 0.033

2.929 ± 0.016

8.993 ± 0.024

2.048 ± 0.013

3.887 ± 0.02

2.496 ± 0.023

10.505 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.702 ± 0.013

2.193 ± 0.019

5.726 ± 0.025

3.131 ± 0.017

7.087 ± 0.03

5.555 ± 0.023

6.362 ± 0.03

8.692 ± 0.027

1.635 ± 0.014

2.251 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski