Tortoise microvirus 80
Average proteome isoelectric point is 5.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8WA79|A0A4P8WA79_9VIRU Uncharacterized protein OS=Tortoise microvirus 80 OX=2583188 PE=4 SV=1
MM1 pKa = 7.71 LFLPILSLSIYY12 pKa = 10.05 LIRR15 pKa = 11.84 RR16 pKa = 11.84 FLMTSKK22 pKa = 10.91 DD23 pKa = 4.26 FIPSSMFGTDD33 pKa = 3.33 DD34 pKa = 3.51 YY35 pKa = 12.25 VNINASIIVDD45 pKa = 3.29 KK46 pKa = 10.93 RR47 pKa = 11.84 LSHH50 pKa = 5.81 VVNSLVSRR58 pKa = 11.84 GKK60 pKa = 10.25 EE61 pKa = 3.9 VFPSLPVSSNPLLANYY77 pKa = 9.6 EE78 pKa = 4.17 YY79 pKa = 10.98 FNAVCSLALYY89 pKa = 10.29 YY90 pKa = 10.64 YY91 pKa = 10.83 FDD93 pKa = 3.8 MLGVLSPSEE102 pKa = 4.22 IAPSTPDD109 pKa = 2.97 VDD111 pKa = 5.69 DD112 pKa = 4.44 DD113 pKa = 4.34 SKK115 pKa = 11.43 YY116 pKa = 9.82 QEE118 pKa = 4.23 EE119 pKa = 4.89 FPFEE123 pKa = 4.17 NYY125 pKa = 9.99
Molecular weight: 14.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.093
IPC2_protein 4.38
IPC_protein 4.279
Toseland 4.088
ProMoST 4.418
Dawson 4.253
Bjellqvist 4.406
Wikipedia 4.177
Rodwell 4.113
Grimsley 3.999
Solomon 4.24
Lehninger 4.202
Nozaki 4.368
DTASelect 4.584
Thurlkill 4.126
EMBOSS 4.177
Sillero 4.393
Patrickios 0.998
IPC_peptide 4.24
IPC2_peptide 4.38
IPC2.peptide.svr19 4.292
Protein with the highest isoelectric point:
>tr|A0A4P8WA79|A0A4P8WA79_9VIRU Uncharacterized protein OS=Tortoise microvirus 80 OX=2583188 PE=4 SV=1
MM1 pKa = 7.26 TLNIRR6 pKa = 11.84 RR7 pKa = 11.84 NFPLKK12 pKa = 9.8 TIRR15 pKa = 11.84 SNPSQPSSTATGRR28 pKa = 11.84 RR29 pKa = 11.84 LIPQRR34 pKa = 11.84 QPAVDD39 pKa = 3.56 SSFSRR44 pKa = 11.84 SEE46 pKa = 3.7 NSLLRR51 pKa = 11.84 GKK53 pKa = 10.41 CPALHH58 pKa = 6.28 CTATPCVSRR67 pKa = 11.84 TYY69 pKa = 11.38 GLRR72 pKa = 11.84 CYY74 pKa = 10.47 CAVQFRR80 pKa = 11.84 ALVAII85 pKa = 4.92
Molecular weight: 9.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.242
IPC2_protein 9.867
IPC_protein 11.111
Toseland 11.155
ProMoST 11.535
Dawson 11.199
Bjellqvist 11.125
Wikipedia 11.608
Rodwell 11.023
Grimsley 11.242
Solomon 11.594
Lehninger 11.506
Nozaki 11.184
DTASelect 11.125
Thurlkill 11.169
EMBOSS 11.637
Sillero 11.184
Patrickios 10.804
IPC_peptide 11.594
IPC2_peptide 10.643
IPC2.peptide.svr19 9.167
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1731
73
571
216.4
24.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.395 ± 1.051
1.444 ± 0.777
6.355 ± 0.452
5.026 ± 1.006
5.373 ± 0.578
5.026 ± 0.492
1.964 ± 0.472
4.622 ± 0.634
4.795 ± 1.118
9.301 ± 0.555
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.6 ± 0.395
4.853 ± 0.384
5.373 ± 0.543
4.622 ± 1.02
5.199 ± 0.648
8.781 ± 0.845
6.355 ± 1.003
5.546 ± 0.599
0.982 ± 0.187
4.391 ± 0.702
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here