Reticulomyxa filosa

Taxonomy: cellular organisms; Eukaryota; Sar; Rhizaria; Retaria; Foraminifera; Monothalamids; Reticulomyxidae; Reticulomyxa

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39903 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X6N2F8|X6N2F8_RETFI Uncharacterized protein OS=Reticulomyxa filosa OX=46433 GN=RFI_16941 PE=4 SV=1
MM1 pKa = 7.37LVDD4 pKa = 3.59TTEE7 pKa = 4.05SPEE10 pKa = 4.36YY11 pKa = 10.62DD12 pKa = 4.57DD13 pKa = 6.2NDD15 pKa = 4.42DD16 pKa = 4.08NDD18 pKa = 4.64DD19 pKa = 3.75VFISTKK25 pKa = 8.06TTDD28 pKa = 3.23EE29 pKa = 4.66GEE31 pKa = 4.56GEE33 pKa = 4.21SSEE36 pKa = 4.75EE37 pKa = 3.99YY38 pKa = 10.76LDD40 pKa = 3.92STSLDD45 pKa = 3.14IDD47 pKa = 3.48TTAATNASWPLDD59 pKa = 2.88WWEE62 pKa = 3.84EE63 pKa = 4.0FIPEE67 pKa = 4.31GFAFTFSLGLEE78 pKa = 4.19GCYY81 pKa = 9.93GVCEE85 pKa = 4.04GVGLGLIWDD94 pKa = 4.04VANQRR99 pKa = 11.84MFLSLDD105 pKa = 3.91SILLGINYY113 pKa = 9.46QKK115 pKa = 10.72GVHH118 pKa = 5.41VFAEE122 pKa = 4.45TGFLLLEE129 pKa = 4.97DD130 pKa = 3.45KK131 pKa = 10.99HH132 pKa = 6.0LTNTYY137 pKa = 10.75LDD139 pKa = 4.73LGWQFDD145 pKa = 3.68LWEE148 pKa = 4.68DD149 pKa = 3.59ANTSVNAVQVNVMWNLGHH167 pKa = 6.42DD168 pKa = 4.11TKK170 pKa = 11.25LLPCGLTLRR179 pKa = 11.84YY180 pKa = 10.18DD181 pKa = 3.2IFGVGSEE188 pKa = 4.47EE189 pKa = 4.42WPCHH193 pKa = 5.65KK194 pKa = 10.47PYY196 pKa = 10.71DD197 pKa = 4.13SPEE200 pKa = 4.12LLDD203 pKa = 4.25EE204 pKa = 5.11GNVSLPAPQLPTNEE218 pKa = 4.32TVSLITTSLNQGTEE232 pKa = 3.53IFSNDD237 pKa = 3.2TQEE240 pKa = 4.33EE241 pKa = 4.86EE242 pKa = 4.33EE243 pKa = 5.01GSTPTQAEE251 pKa = 4.32LMTTVAEE258 pKa = 4.24SLPNGSVSQPPTTPNLTAVEE278 pKa = 4.54EE279 pKa = 4.62GQSTDD284 pKa = 3.71LPSEE288 pKa = 4.24FPLTSIIDD296 pKa = 3.73LPPWFEE302 pKa = 3.93APYY305 pKa = 10.56CEE307 pKa = 4.21GFRR310 pKa = 11.84VSLRR314 pKa = 11.84RR315 pKa = 11.84KK316 pKa = 9.45KK317 pKa = 10.62VLAKK321 pKa = 9.15MDD323 pKa = 4.13CYY325 pKa = 11.42

Molecular weight: 36.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X6L8J3|X6L8J3_RETFI Uncharacterized protein (Fragment) OS=Reticulomyxa filosa OX=46433 GN=RFI_39203 PE=4 SV=1
GG1 pKa = 7.23RR2 pKa = 11.84FVGLFVGILVGFLVGKK18 pKa = 9.54FVGLLVGTFVGLFVGRR34 pKa = 11.84FVGLFVGTLVGLFVGTVVGLLVGTFVGLFVGTLVGLFVGILVGLLVGTLVGLFVGTLVGLLVGTFVGLFVGRR106 pKa = 11.84LVGLLVGTLVGLFVGILVGLFVGTLVGLFVGILVGLLVGTLVGLLVGTLVGLLVGTFVGLFVGRR170 pKa = 11.84LVGLLVGTLVGLFVGTLVGLFVGIFVGLLVGTFVGLFVGSLVVGLLVGTLVGLFVGTFVGLFVGRR235 pKa = 11.84LL236 pKa = 3.33

Molecular weight: 23.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39903

0

39903

12687417

33

4041

318.0

36.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.668 ± 0.011

2.069 ± 0.007

5.563 ± 0.01

6.624 ± 0.016

4.909 ± 0.01

4.277 ± 0.012

2.769 ± 0.008

6.6 ± 0.015

8.779 ± 0.019

8.852 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.006

6.864 ± 0.016

3.232 ± 0.009

4.886 ± 0.011

4.175 ± 0.009

7.689 ± 0.014

5.559 ± 0.011

5.489 ± 0.01

1.297 ± 0.005

3.369 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski