Enterococcus ratti
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2411 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L8WQ76|A0A1L8WQ76_9ENTE PRD domain-containing protein OS=Enterococcus ratti OX=150033 GN=RV14_GL001869 PE=4 SV=1
MM1 pKa = 7.52 KK2 pKa = 10.01 RR3 pKa = 11.84 GKK5 pKa = 10.89 LMTEE9 pKa = 3.89 NNHH12 pKa = 5.09 THH14 pKa = 6.65 DD15 pKa = 4.71 HH16 pKa = 5.89 DD17 pKa = 4.91 HH18 pKa = 7.09 EE19 pKa = 4.45 GHH21 pKa = 5.2 EE22 pKa = 5.0 HH23 pKa = 5.6 ITLVDD28 pKa = 3.67 DD29 pKa = 4.08 QGNEE33 pKa = 3.72 TLYY36 pKa = 10.96 EE37 pKa = 3.92 ILLTVDD43 pKa = 3.27 GQEE46 pKa = 4.0 EE47 pKa = 4.22 FGRR50 pKa = 11.84 NYY52 pKa = 10.54 VLLYY56 pKa = 9.2 PAGVSEE62 pKa = 5.2 DD63 pKa = 3.8 EE64 pKa = 4.43 DD65 pKa = 4.35 VEE67 pKa = 4.31 LQAYY71 pKa = 9.86 AYY73 pKa = 9.68 IEE75 pKa = 4.46 NEE77 pKa = 4.25 DD78 pKa = 3.63 GTEE81 pKa = 4.16 GEE83 pKa = 4.51 LEE85 pKa = 4.16 QIEE88 pKa = 4.57 TDD90 pKa = 4.13 AEE92 pKa = 3.62 WDD94 pKa = 3.57 MIEE97 pKa = 4.38 EE98 pKa = 4.22 VFNTFMAEE106 pKa = 3.9 EE107 pKa = 4.15 EE108 pKa = 4.24
Molecular weight: 12.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.791
IPC2_protein 3.948
IPC_protein 3.872
Toseland 3.706
ProMoST 3.973
Dawson 3.834
Bjellqvist 4.024
Wikipedia 3.694
Rodwell 3.719
Grimsley 3.617
Solomon 3.821
Lehninger 3.77
Nozaki 3.948
DTASelect 4.062
Thurlkill 3.732
EMBOSS 3.719
Sillero 3.986
Patrickios 1.863
IPC_peptide 3.821
IPC2_peptide 3.973
IPC2.peptide.svr19 3.898
Protein with the highest isoelectric point:
>tr|A0A1L8WKZ6|A0A1L8WKZ6_9ENTE Uncharacterized protein OS=Enterococcus ratti OX=150033 GN=RV14_GL000222 PE=4 SV=1
MM1 pKa = 7.92 PKK3 pKa = 10.12 QKK5 pKa = 7.54 THH7 pKa = 6.73 RR8 pKa = 11.84 GSAKK12 pKa = 9.62 RR13 pKa = 11.84 FKK15 pKa = 10.04 RR16 pKa = 11.84 TGKK19 pKa = 10.53 GGLKK23 pKa = 9.97 RR24 pKa = 11.84 FRR26 pKa = 11.84 AFTSHH31 pKa = 6.94 RR32 pKa = 11.84 FHH34 pKa = 7.48 GKK36 pKa = 6.37 TKK38 pKa = 9.57 KK39 pKa = 9.43 QRR41 pKa = 11.84 RR42 pKa = 11.84 QLRR45 pKa = 11.84 KK46 pKa = 9.6 ASIVSSGDD54 pKa = 3.16 FKK56 pKa = 11.25 RR57 pKa = 11.84 IRR59 pKa = 11.84 QQLAKK64 pKa = 9.86 MKK66 pKa = 10.54
Molecular weight: 7.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 10.95
IPC_protein 12.501
Toseland 12.676
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.544
Grimsley 12.72
Solomon 13.159
Lehninger 13.056
Nozaki 12.676
DTASelect 12.661
Thurlkill 12.676
EMBOSS 13.173
Sillero 12.676
Patrickios 12.266
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2411
0
2411
662122
37
1715
274.6
31.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.602 ± 0.049
0.693 ± 0.014
4.671 ± 0.038
7.359 ± 0.06
4.719 ± 0.042
6.158 ± 0.048
1.895 ± 0.023
7.932 ± 0.047
7.956 ± 0.053
9.968 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.694 ± 0.025
4.858 ± 0.04
3.261 ± 0.022
4.383 ± 0.04
3.902 ± 0.041
5.901 ± 0.039
5.734 ± 0.039
6.596 ± 0.041
0.9 ± 0.017
3.82 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here