Acidianus bottle-shaped virus 3

Taxonomy: Viruses; Ampullaviridae; Bottigliavirus; Bottigliavirus ABV3

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9P9A0|A0A0N9P9A0_9VIRU Uncharacterized protein OS=Acidianus bottle-shaped virus 3 OX=1732174 PE=4 SV=1
MM1 pKa = 8.04LDD3 pKa = 3.68TILFIAGGLLFSNTFMGVIIYY24 pKa = 10.01IEE26 pKa = 4.19IKK28 pKa = 9.68KK29 pKa = 9.19MEE31 pKa = 4.21YY32 pKa = 10.35VFEE35 pKa = 4.53QILQGISTNNQNDD48 pKa = 3.75EE49 pKa = 4.15EE50 pKa = 6.23DD51 pKa = 4.04YY52 pKa = 11.57DD53 pKa = 4.77PDD55 pKa = 4.0TNSYY59 pKa = 9.61II60 pKa = 3.9

Molecular weight: 6.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9NWB6|A0A0N9NWB6_9VIRU Uncharacterized protein OS=Acidianus bottle-shaped virus 3 OX=1732174 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.36SLRR5 pKa = 11.84KK6 pKa = 9.69VKK8 pKa = 10.48RR9 pKa = 11.84KK10 pKa = 10.16LKK12 pKa = 10.79DD13 pKa = 2.89KK14 pKa = 11.21GITIKK19 pKa = 10.47IKK21 pKa = 10.25PDD23 pKa = 3.2TLFEE27 pKa = 4.0FLFAFFIGNLVLTKK41 pKa = 10.95NFIQSLYY48 pKa = 7.48STGYY52 pKa = 8.8AFVYY56 pKa = 10.06VLIGTLVLFGVIVGILFFFYY76 pKa = 11.12LLGSLLRR83 pKa = 4.87

Molecular weight: 9.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

8675

38

662

131.4

15.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.104 ± 0.259

1.568 ± 0.29

5.037 ± 0.322

7.643 ± 0.424

5.153 ± 0.293

3.988 ± 0.319

1.383 ± 0.156

9.245 ± 0.291

8.622 ± 0.649

11.159 ± 0.454

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.225 ± 0.216

5.695 ± 0.333

4.161 ± 0.439

3.47 ± 0.336

2.767 ± 0.252

6.006 ± 0.367

5.037 ± 0.181

6.571 ± 0.3

0.715 ± 0.102

5.452 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski