Cherry chlorotic rusty spot associated partitivirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Alphapartitivirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q65A70|Q65A70_9VIRU RNA-dependent RNA polymerase OS=Cherry chlorotic rusty spot associated partitivirus OX=284688 GN=RdRp PE=4 SV=1
MM1 pKa = 7.53SAPATTGSTTSTASVPATEE20 pKa = 4.18QTAPASVAVPPTAPATDD37 pKa = 3.48VKK39 pKa = 11.1YY40 pKa = 10.55VAPAKK45 pKa = 9.2KK46 pKa = 9.62QKK48 pKa = 10.55SFAPRR53 pKa = 11.84EE54 pKa = 4.04PTATSGGPKK63 pKa = 10.22NPGLSMMLSGVSDD76 pKa = 3.86LPFFGIKK83 pKa = 10.24HH84 pKa = 6.16NDD86 pKa = 2.93VSYY89 pKa = 10.93VVPDD93 pKa = 3.91TTQLFYY99 pKa = 11.31VLSIMDD105 pKa = 3.58TQMARR110 pKa = 11.84TKK112 pKa = 10.5RR113 pKa = 11.84FTDD116 pKa = 4.45ANPDD120 pKa = 2.97WHH122 pKa = 6.9PFVSQLYY129 pKa = 8.84IAVLFYY135 pKa = 11.06YY136 pKa = 10.1QVLKK140 pKa = 10.84NQTHH144 pKa = 6.61GGMISNDD151 pKa = 2.7QRR153 pKa = 11.84LFVDD157 pKa = 5.56FLDD160 pKa = 3.91TQFKK164 pKa = 10.92AEE166 pKa = 4.2HH167 pKa = 6.27LKK169 pKa = 10.47IPGPIAIFFQSLAANAGPNEE189 pKa = 4.06NFGNLVFGIPNAHH202 pKa = 7.31DD203 pKa = 3.69ISCTSYY209 pKa = 9.09LWQDD213 pKa = 3.15KK214 pKa = 9.18VHH216 pKa = 6.9TILPNVIFILDD227 pKa = 3.29QFMRR231 pKa = 11.84LISLISPVNSGPVQANASHH250 pKa = 7.08TDD252 pKa = 3.31TVYY255 pKa = 8.22TTIFGAPASKK265 pKa = 11.03DD266 pKa = 3.3EE267 pKa = 4.14ATRR270 pKa = 11.84FAMLTPSARR279 pKa = 11.84SDD281 pKa = 3.44FQTTVGLLNGLSTSSNVWRR300 pKa = 11.84NTLPFANDD308 pKa = 3.2GDD310 pKa = 4.13SQYY313 pKa = 11.63VITEE317 pKa = 4.07DD318 pKa = 5.92DD319 pKa = 3.77EE320 pKa = 6.03LLDD323 pKa = 4.13LDD325 pKa = 3.93QVFGFRR331 pKa = 11.84GIGPYY336 pKa = 10.36ADD338 pKa = 4.61RR339 pKa = 11.84PYY341 pKa = 11.27GWFAQVIRR349 pKa = 11.84VMQPYY354 pKa = 10.75SDD356 pKa = 4.09FFKK359 pKa = 10.59DD360 pKa = 3.71TVSLGSVTTTGTGISYY376 pKa = 10.28IRR378 pKa = 11.84TKK380 pKa = 11.1YY381 pKa = 10.99VDD383 pKa = 3.15TKK385 pKa = 9.78QNKK388 pKa = 9.08NILVHH393 pKa = 6.68AVTTRR398 pKa = 11.84KK399 pKa = 9.29VRR401 pKa = 11.84YY402 pKa = 6.57QTGSTLRR409 pKa = 11.84YY410 pKa = 9.01HH411 pKa = 6.95IPEE414 pKa = 3.94FTGITTIHH422 pKa = 6.01LHH424 pKa = 6.67AEE426 pKa = 4.04EE427 pKa = 4.71FLDD430 pKa = 5.47LVTEE434 pKa = 4.06QAGILTQLNSDD445 pKa = 3.05WTDD448 pKa = 2.76INKK451 pKa = 10.15DD452 pKa = 3.45VTDD455 pKa = 3.9TSGPDD460 pKa = 3.26YY461 pKa = 10.59TSTHH465 pKa = 5.87VGPIFSIPDD474 pKa = 3.35SGRR477 pKa = 11.84TSQYY481 pKa = 10.6KK482 pKa = 9.8VSNTIAPLISGYY494 pKa = 9.37YY495 pKa = 7.17HH496 pKa = 6.47TPSALRR502 pKa = 11.84FEE504 pKa = 4.67

Molecular weight: 55.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q65A70|Q65A70_9VIRU RNA-dependent RNA polymerase OS=Cherry chlorotic rusty spot associated partitivirus OX=284688 GN=RdRp PE=4 SV=1
MM1 pKa = 7.85DD2 pKa = 5.56HH3 pKa = 6.36LTSLFEE9 pKa = 4.7LFAITPKK16 pKa = 9.25TQNNLQFVGIYY27 pKa = 9.49HH28 pKa = 7.2RR29 pKa = 11.84PPHH32 pKa = 5.77SVRR35 pKa = 11.84ANLRR39 pKa = 11.84NVEE42 pKa = 4.02KK43 pKa = 10.89HH44 pKa = 6.1KK45 pKa = 10.22ITVAHH50 pKa = 6.85AMHH53 pKa = 7.1KK54 pKa = 9.95YY55 pKa = 10.33LYY57 pKa = 8.6PHH59 pKa = 7.31EE60 pKa = 4.72IDD62 pKa = 4.64FVINQMRR69 pKa = 11.84RR70 pKa = 11.84SNVTEE75 pKa = 3.91DD76 pKa = 4.65AILADD81 pKa = 4.1FFDD84 pKa = 4.77NNVEE88 pKa = 3.98PLEE91 pKa = 4.37PVLDD95 pKa = 3.67EE96 pKa = 4.21HH97 pKa = 7.36FEE99 pKa = 4.31RR100 pKa = 11.84GLSAMLDD107 pKa = 3.51AFRR110 pKa = 11.84PPQKK114 pKa = 10.17CLPAHH119 pKa = 6.79IYY121 pKa = 9.98DD122 pKa = 4.15VQHH125 pKa = 6.75HH126 pKa = 6.33YY127 pKa = 9.93PYY129 pKa = 10.23KK130 pKa = 8.07WQVNAEE136 pKa = 4.32APFSTDD142 pKa = 3.2SYY144 pKa = 11.38FLANRR149 pKa = 11.84PTFRR153 pKa = 11.84AVFEE157 pKa = 4.34RR158 pKa = 11.84LEE160 pKa = 3.87SLYY163 pKa = 10.24THH165 pKa = 7.79LATDD169 pKa = 2.74WHH171 pKa = 5.95RR172 pKa = 11.84RR173 pKa = 11.84YY174 pKa = 10.71GNKK177 pKa = 8.83TDD179 pKa = 5.12NDD181 pKa = 4.14DD182 pKa = 3.93FMNDD186 pKa = 3.05HH187 pKa = 6.34VPAKK191 pKa = 9.77FGPMKK196 pKa = 9.67EE197 pKa = 4.61TVFSWTHH204 pKa = 5.21RR205 pKa = 11.84WHH207 pKa = 6.89HH208 pKa = 5.56VIKK211 pKa = 11.19SNFTDD216 pKa = 3.39TAGLSKK222 pKa = 10.64DD223 pKa = 3.67YY224 pKa = 10.71YY225 pKa = 10.71FKK227 pKa = 11.1NRR229 pKa = 11.84YY230 pKa = 7.77IFPMLLHH237 pKa = 6.66TKK239 pKa = 8.25TAIVKK244 pKa = 10.22KK245 pKa = 10.36DD246 pKa = 3.95DD247 pKa = 4.07PNKK250 pKa = 9.8MRR252 pKa = 11.84TIWGCSKK259 pKa = 10.15PWIIADD265 pKa = 3.57TMLWWEE271 pKa = 4.0YY272 pKa = 10.05VAYY275 pKa = 10.38AKK277 pKa = 9.55LQPGATPMLWSYY289 pKa = 8.05EE290 pKa = 4.16TFTGGWLRR298 pKa = 11.84LNHH301 pKa = 6.83ALFSSYY307 pKa = 9.7IRR309 pKa = 11.84HH310 pKa = 6.34SYY312 pKa = 7.64ITLDD316 pKa = 3.11WKK318 pKa = 11.11RR319 pKa = 11.84FDD321 pKa = 3.44KK322 pKa = 10.8KK323 pKa = 11.06AYY325 pKa = 9.7FCIIDD330 pKa = 3.92KK331 pKa = 10.84IFDD334 pKa = 3.79GVEE337 pKa = 3.79TFLDD341 pKa = 3.87FDD343 pKa = 3.92NGYY346 pKa = 10.21LPTKK350 pKa = 9.96DD351 pKa = 3.85YY352 pKa = 11.13PDD354 pKa = 3.92TKK356 pKa = 10.46STWTQGRR363 pKa = 11.84STRR366 pKa = 11.84IKK368 pKa = 10.71RR369 pKa = 11.84LFDD372 pKa = 3.04WTKK375 pKa = 10.07EE376 pKa = 4.22NFYY379 pKa = 10.19HH380 pKa = 7.28APIVLPNGHH389 pKa = 6.23MYY391 pKa = 9.8VRR393 pKa = 11.84QFAGIPSGLFITQLIDD409 pKa = 2.46SWYY412 pKa = 9.4NYY414 pKa = 9.25TMLATILSAMGFDD427 pKa = 3.97PRR429 pKa = 11.84SCIIKK434 pKa = 9.98VQGDD438 pKa = 3.52DD439 pKa = 3.9SIIRR443 pKa = 11.84LSALIPPDD451 pKa = 3.22AHH453 pKa = 7.43DD454 pKa = 4.05SFLTKK459 pKa = 10.32VQEE462 pKa = 3.84LADD465 pKa = 3.93YY466 pKa = 9.34YY467 pKa = 9.39FQSVVSVNKK476 pKa = 10.38SEE478 pKa = 4.66VRR480 pKa = 11.84NEE482 pKa = 3.87LNGCEE487 pKa = 4.1VLSYY491 pKa = 9.85RR492 pKa = 11.84HH493 pKa = 5.67RR494 pKa = 11.84HH495 pKa = 4.39GLPYY499 pKa = 9.63RR500 pKa = 11.84DD501 pKa = 4.09EE502 pKa = 4.56LAMLAQLYY510 pKa = 7.18HH511 pKa = 5.81TKK513 pKa = 10.5ARR515 pKa = 11.84NPSPEE520 pKa = 3.35ITMAQSIGFAYY531 pKa = 10.29ASFGNHH537 pKa = 3.93EE538 pKa = 4.43RR539 pKa = 11.84VRR541 pKa = 11.84LVLHH545 pKa = 7.38DD546 pKa = 3.11IYY548 pKa = 10.81EE549 pKa = 4.5YY550 pKa = 11.04YY551 pKa = 10.29KK552 pKa = 10.56HH553 pKa = 6.51QGYY556 pKa = 7.43TPNRR560 pKa = 11.84AGLSLVFGNSPDD572 pKa = 4.23LMIPHH577 pKa = 6.13YY578 pKa = 9.56TLDD581 pKa = 3.53HH582 pKa = 6.51FPTLRR587 pKa = 11.84EE588 pKa = 3.4IKK590 pKa = 10.12MFLTNANYY598 pKa = 10.78ANEE601 pKa = 4.31EE602 pKa = 4.45TNSRR606 pKa = 11.84TWPLNHH612 pKa = 6.56FLSLPCHH619 pKa = 5.54RR620 pKa = 11.84TT621 pKa = 3.18

Molecular weight: 72.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1125

504

621

562.5

64.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.756 ± 0.395

0.622 ± 0.286

6.4 ± 0.099

3.467 ± 0.732

5.956 ± 0.136

4.889 ± 0.85

4.089 ± 1.017

5.689 ± 0.178

4.533 ± 0.515

8.089 ± 0.638

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.311 ± 0.354

4.889 ± 0.353

6.222 ± 0.353

3.467 ± 0.739

4.622 ± 0.842

6.844 ± 1.003

8.622 ± 1.544

5.778 ± 0.92

1.778 ± 0.53

4.978 ± 0.68
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski