Candidatus Methanoperedens nitroreducens

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Candidatus Methanoperedenaceae; Candidatus Methanoperedens

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3430 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A062UV42|A0A062UV42_9EURY Replication factor C small subunit OS=Candidatus Methanoperedens nitroreducens OX=1392998 GN=rfcS PE=3 SV=1
MM1 pKa = 6.99TGVVATADD9 pKa = 3.79TEE11 pKa = 4.38AVLIDD16 pKa = 3.75NSTEE20 pKa = 3.81HH21 pKa = 7.14RR22 pKa = 11.84DD23 pKa = 3.52DD24 pKa = 4.22YY25 pKa = 11.67FDD27 pKa = 4.17VDD29 pKa = 3.58TKK31 pKa = 10.87IYY33 pKa = 10.56TEE35 pKa = 4.55GPNLSPSTSYY45 pKa = 11.71NLTITDD51 pKa = 3.59QGSSGNQRR59 pKa = 11.84SIVAQDD65 pKa = 3.71EE66 pKa = 4.61GAMDD70 pKa = 5.66PDD72 pKa = 3.52TTYY75 pKa = 10.52IIPEE79 pKa = 4.41FPTIALPVAAALGIIFIIYY98 pKa = 9.89NRR100 pKa = 11.84IIEE103 pKa = 4.24EE104 pKa = 4.0

Molecular weight: 11.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A062V3C4|A0A062V3C4_9EURY Putative methyltransferase OS=Candidatus Methanoperedens nitroreducens OX=1392998 GN=ANME2D_03440 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84KK3 pKa = 8.83KK4 pKa = 10.47QKK6 pKa = 9.97GWAKK10 pKa = 11.07DD11 pKa = 3.28MAQQRR16 pKa = 11.84IIRR19 pKa = 11.84LFEE22 pKa = 4.17LADD25 pKa = 3.76HH26 pKa = 6.53EE27 pKa = 5.2FKK29 pKa = 11.03AHH31 pKa = 6.32PEE33 pKa = 3.57RR34 pKa = 11.84SNRR37 pKa = 11.84YY38 pKa = 7.54VQLARR43 pKa = 11.84RR44 pKa = 11.84IAMRR48 pKa = 11.84YY49 pKa = 8.12RR50 pKa = 11.84VRR52 pKa = 11.84IPQEE56 pKa = 3.96LKK58 pKa = 10.54QKK60 pKa = 9.23VCKK63 pKa = 9.81HH64 pKa = 4.62CHH66 pKa = 5.83AYY68 pKa = 10.21LVQGVTARR76 pKa = 11.84TRR78 pKa = 11.84LQDD81 pKa = 3.13THH83 pKa = 6.33ITTTCLVCGEE93 pKa = 4.09QMRR96 pKa = 11.84RR97 pKa = 11.84PYY99 pKa = 11.0

Molecular weight: 11.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3421

9

3430

941967

8

3539

274.6

30.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.123 ± 0.043

1.124 ± 0.024

5.587 ± 0.035

7.098 ± 0.06

4.029 ± 0.033

7.164 ± 0.046

1.825 ± 0.02

8.818 ± 0.049

6.476 ± 0.046

9.173 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.587 ± 0.024

4.283 ± 0.049

3.957 ± 0.029

2.559 ± 0.025

5.338 ± 0.036

6.312 ± 0.041

5.355 ± 0.054

6.683 ± 0.035

0.994 ± 0.018

3.515 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski