Turkey adenovirus 3
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9YUQ3|Q9YUQ3_9ADEN ORF7 OS=Turkey adenovirus 3 OX=41678 GN=ORF7 PE=4 SV=1
MM1 pKa = 8.16 DD2 pKa = 4.0 VGEE5 pKa = 4.77 SDD7 pKa = 4.68 NEE9 pKa = 4.1 VEE11 pKa = 4.63 DD12 pKa = 5.4 RR13 pKa = 11.84 RR14 pKa = 11.84 IQVPKK19 pKa = 10.14 VHH21 pKa = 6.04 STNPFSSGSRR31 pKa = 11.84 EE32 pKa = 4.08 TMPTPSSTRR41 pKa = 11.84 FVYY44 pKa = 10.12 VLKK47 pKa = 10.23 TEE49 pKa = 4.42 DD50 pKa = 4.6 DD51 pKa = 3.68 EE52 pKa = 5.53 TIMLEE57 pKa = 3.87 NTGTFKK63 pKa = 10.89 LSEE66 pKa = 4.23 YY67 pKa = 11.0 NFGKK71 pKa = 10.63 LFDD74 pKa = 4.98 DD75 pKa = 4.37 FDD77 pKa = 4.32 VVTLEE82 pKa = 3.92 MSLGFHH88 pKa = 7.1 YY89 pKa = 10.43 MFDD92 pKa = 3.46 YY93 pKa = 10.55 MLDD96 pKa = 3.92 VKK98 pKa = 9.99 WPNVDD103 pKa = 4.44 FEE105 pKa = 4.81 YY106 pKa = 10.64 TVNVYY111 pKa = 7.27 TEE113 pKa = 4.02 RR114 pKa = 11.84 RR115 pKa = 11.84 IALYY119 pKa = 10.34 DD120 pKa = 4.15 LEE122 pKa = 6.49 NIDD125 pKa = 3.73 YY126 pKa = 8.17 WPCVLAWCAFQCISNGVNFFDD147 pKa = 3.86 HH148 pKa = 6.01 VRR150 pKa = 11.84 LVGEE154 pKa = 4.39 DD155 pKa = 3.11 HH156 pKa = 6.67 SLPYY160 pKa = 9.75 GTVFAA165 pKa = 5.64
Molecular weight: 19.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.122
IPC2_protein 4.304
IPC_protein 4.253
Toseland 4.075
ProMoST 4.38
Dawson 4.215
Bjellqvist 4.368
Wikipedia 4.113
Rodwell 4.088
Grimsley 3.986
Solomon 4.215
Lehninger 4.164
Nozaki 4.329
DTASelect 4.52
Thurlkill 4.101
EMBOSS 4.139
Sillero 4.368
Patrickios 2.765
IPC_peptide 4.215
IPC2_peptide 4.355
IPC2.peptide.svr19 4.266
Protein with the highest isoelectric point:
>tr|Q9YUR9|Q9YUR9_9ADEN Uncharacterized protein OS=Turkey adenovirus 3 OX=41678 PE=4 SV=1
MM1 pKa = 7.79 PFFYY5 pKa = 10.74 LVGAGSAYY13 pKa = 10.27 CEE15 pKa = 4.03 RR16 pKa = 11.84 CKK18 pKa = 10.38 EE19 pKa = 4.08 VCEE22 pKa = 4.06 KK23 pKa = 10.3 RR24 pKa = 11.84 RR25 pKa = 11.84 KK26 pKa = 9.81 RR27 pKa = 11.84 STTRR31 pKa = 11.84 TTRR34 pKa = 11.84 TKK36 pKa = 10.31 RR37 pKa = 11.84 SKK39 pKa = 10.18 PSHH42 pKa = 5.55 LQYY45 pKa = 10.64 VRR47 pKa = 11.84 YY48 pKa = 9.81 YY49 pKa = 8.68 PGTVVPVGWDD59 pKa = 3.46 GTDD62 pKa = 3.14 KK63 pKa = 10.78 PVTVTRR69 pKa = 11.84 IPDD72 pKa = 2.97 YY73 pKa = 8.46 WTYY76 pKa = 11.77 DD77 pKa = 3.18 RR78 pKa = 11.84 AVSSRR83 pKa = 11.84 QSNTVVPVNTSGEE96 pKa = 4.23 NPTVVAVPRR105 pKa = 11.84 LLRR108 pKa = 11.84 KK109 pKa = 9.98 RR110 pKa = 11.84 KK111 pKa = 10.05 ASDD114 pKa = 2.99 MM115 pKa = 4.12
Molecular weight: 13.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.677
IPC_protein 10.204
Toseland 10.248
ProMoST 9.999
Dawson 10.452
Bjellqvist 10.175
Wikipedia 10.657
Rodwell 10.745
Grimsley 10.54
Solomon 10.496
Lehninger 10.452
Nozaki 10.277
DTASelect 10.16
Thurlkill 10.306
EMBOSS 10.657
Sillero 10.379
Patrickios 10.379
IPC_peptide 10.496
IPC2_peptide 9.18
IPC2.peptide.svr19 8.559
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24
0
24
8362
58
1112
348.4
39.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.831 ± 0.409
2.069 ± 0.397
5.071 ± 0.317
5.023 ± 0.497
5.489 ± 0.314
5.071 ± 0.508
1.686 ± 0.173
6.673 ± 0.544
5.812 ± 0.712
9.663 ± 0.53
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.452 ± 0.255
6.745 ± 0.395
5.417 ± 0.266
3.899 ± 0.31
4.52 ± 0.526
7.905 ± 0.359
6.601 ± 0.461
5.872 ± 0.492
1.136 ± 0.094
4.066 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here