Escherichia phage K1E (Bacteriophage K1E)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Vectrevirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2WC17|Q2WC17_BPK1E Gp11 protein OS=Escherichia phage K1E OX=344022 GN=11 PE=4 SV=1
MM1 pKa = 7.4PRR3 pKa = 11.84DD4 pKa = 3.79YY5 pKa = 11.54DD6 pKa = 4.34SDD8 pKa = 3.47WDD10 pKa = 4.19FQDD13 pKa = 3.66SMNSKK18 pKa = 10.45PEE20 pKa = 3.87RR21 pKa = 11.84SDD23 pKa = 4.52DD24 pKa = 3.96YY25 pKa = 11.76YY26 pKa = 10.44EE27 pKa = 4.17TEE29 pKa = 4.66AMYY32 pKa = 10.66EE33 pKa = 4.31SYY35 pKa = 11.47

Molecular weight: 4.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2WC13|Q2WC13_BPK1E Gp13.1 protein OS=Escherichia phage K1E OX=344022 GN=13.1 PE=4 SV=1
MM1 pKa = 7.79KK2 pKa = 10.62SPLLYY7 pKa = 10.38GKK9 pKa = 10.06RR10 pKa = 11.84EE11 pKa = 3.74PHH13 pKa = 6.01VKK15 pKa = 10.12VKK17 pKa = 10.55QSLIGAWVSLHH28 pKa = 5.27GTYY31 pKa = 10.11SYY33 pKa = 10.78PVVVRR38 pKa = 11.84SSTVVTLKK46 pKa = 10.12PSTRR50 pKa = 11.84RR51 pKa = 11.84GCGLRR56 pKa = 11.84KK57 pKa = 9.45LVYY60 pKa = 9.75EE61 pKa = 4.47SVAALYY67 pKa = 10.27LL68 pKa = 3.92

Molecular weight: 7.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13938

32

1102

224.8

25.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.832 ± 0.529

1.105 ± 0.188

6.113 ± 0.239

7.239 ± 0.375

3.638 ± 0.148

7.419 ± 0.348

2.181 ± 0.269

5.46 ± 0.233

6.78 ± 0.299

7.871 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.279 ± 0.187

4.003 ± 0.174

3.623 ± 0.179

4.212 ± 0.29

5.467 ± 0.26

5.639 ± 0.234

5.474 ± 0.216

6.658 ± 0.263

1.428 ± 0.142

3.58 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski