Mitsuaria sp. 7

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Mitsuaria; unclassified Mitsuaria

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4769 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A9HQG8|A0A1A9HQG8_9BURK 4-oxalomesaconate hydratase OS=Mitsuaria sp. 7 OX=1658665 GN=ABE85_18980 PE=4 SV=1
MM1 pKa = 7.84TDD3 pKa = 3.31VQSDD7 pKa = 3.67TAAAPASPEE16 pKa = 4.21AGDD19 pKa = 4.98DD20 pKa = 3.89PMPVPPPQPDD30 pKa = 2.87IDD32 pKa = 3.9ACCGNGCDD40 pKa = 3.55PCIFDD45 pKa = 4.89LHH47 pKa = 6.93DD48 pKa = 4.08LAMDD52 pKa = 4.88AYY54 pKa = 9.88RR55 pKa = 11.84QALRR59 pKa = 11.84AWKK62 pKa = 9.41VRR64 pKa = 11.84HH65 pKa = 6.41PGTPP69 pKa = 3.01

Molecular weight: 7.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A9HMW0|A0A1A9HMW0_9BURK Abhydrolase_3 domain-containing protein OS=Mitsuaria sp. 7 OX=1658665 GN=ABE85_18610 PE=3 SV=1
MM1 pKa = 7.78DD2 pKa = 5.65ASRR5 pKa = 11.84ATPVRR10 pKa = 11.84RR11 pKa = 11.84AHH13 pKa = 6.41RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84SIRR19 pKa = 11.84GRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84SGRR26 pKa = 11.84PRR28 pKa = 11.84RR29 pKa = 11.84SASRR33 pKa = 11.84TRR35 pKa = 11.84APRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84TSPDD44 pKa = 2.31THH46 pKa = 4.66TTPRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84PWTRR58 pKa = 11.84PPSGRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84WRR67 pKa = 11.84PWRR70 pKa = 11.84RR71 pKa = 11.84GRR73 pKa = 11.84CSGPRR78 pKa = 11.84RR79 pKa = 11.84PQRR82 pKa = 11.84GNTT85 pKa = 3.35

Molecular weight: 10.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4769

0

4769

1584249

44

5906

332.2

35.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.903 ± 0.049

0.802 ± 0.01

5.681 ± 0.024

5.25 ± 0.031

3.362 ± 0.02

8.509 ± 0.04

2.149 ± 0.019

3.967 ± 0.026

3.095 ± 0.028

10.894 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.333 ± 0.016

2.416 ± 0.028

5.395 ± 0.026

3.938 ± 0.02

7.319 ± 0.04

5.554 ± 0.029

5.293 ± 0.026

7.506 ± 0.029

1.556 ± 0.014

2.076 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski