Coniothyrium minitans RNA virus
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8B5B7|Q8B5B7_9VIRU RNA-directed RNA polymerase OS=Coniothyrium minitans RNA virus OX=210827 PE=3 SV=1
MM1 pKa = 7.53 ASSTRR6 pKa = 11.84 FLASAASPLTGTVAGISAGTIGQDD30 pKa = 3.04 NQYY33 pKa = 10.34 RR34 pKa = 11.84 RR35 pKa = 11.84 YY36 pKa = 9.92 RR37 pKa = 11.84 AGLTIGVHH45 pKa = 4.54 EE46 pKa = 5.09 HH47 pKa = 6.62 GSYY50 pKa = 8.47 TQARR54 pKa = 11.84 RR55 pKa = 11.84 SIFYY59 pKa = 10.28 EE60 pKa = 3.51 VGRR63 pKa = 11.84 RR64 pKa = 11.84 YY65 pKa = 10.5 GRR67 pKa = 11.84 LTDD70 pKa = 3.53 ALGPHH75 pKa = 6.49 GAEE78 pKa = 4.25 AVPIDD83 pKa = 3.43 ASVRR87 pKa = 11.84 INAAEE92 pKa = 3.86 AANFEE97 pKa = 4.0 GFARR101 pKa = 11.84 KK102 pKa = 9.39 FSNFSPQWLTMDD114 pKa = 4.02 LCGIAEE120 pKa = 4.11 RR121 pKa = 11.84 LAKK124 pKa = 10.15 GVAAQSVYY132 pKa = 11.16 GGVNIVNLRR141 pKa = 11.84 GHH143 pKa = 6.39 LPVRR147 pKa = 11.84 VVALGTLDD155 pKa = 4.0 SPQTASNNSVFIPRR169 pKa = 11.84 TVDD172 pKa = 3.11 TVGNDD177 pKa = 2.94 HH178 pKa = 6.29 VFAVLAAAANGEE190 pKa = 4.2 GAAVSTDD197 pKa = 3.53 VLRR200 pKa = 11.84 LDD202 pKa = 4.43 ANTNEE207 pKa = 4.11 PVIPAVSGPSLASACIEE224 pKa = 3.77 ALRR227 pKa = 11.84 IVGANMEE234 pKa = 4.26 ASGAGDD240 pKa = 3.99 LFAYY244 pKa = 10.12 AVTRR248 pKa = 11.84 GIHH251 pKa = 5.54 AVVSVVAHH259 pKa = 5.89 TDD261 pKa = 2.67 EE262 pKa = 4.79 GGYY265 pKa = 8.74 MRR267 pKa = 11.84 ALLRR271 pKa = 11.84 HH272 pKa = 4.93 GRR274 pKa = 11.84 FRR276 pKa = 11.84 VPYY279 pKa = 10.14 GGINQALRR287 pKa = 11.84 DD288 pKa = 4.12 YY289 pKa = 9.94 PALPAAGALATHH301 pKa = 6.96 VISSWVDD308 pKa = 3.93 AIALKK313 pKa = 9.09 TAAVVAHH320 pKa = 6.79 SDD322 pKa = 3.37 PCVIASGGLYY332 pKa = 7.65 PTVFTSSQGDD342 pKa = 3.42 ITPPGTDD349 pKa = 3.65 EE350 pKa = 4.94 GDD352 pKa = 3.69 SPTDD356 pKa = 3.2 ADD358 pKa = 3.3 ARR360 pKa = 11.84 AIGRR364 pKa = 11.84 QIAGDD369 pKa = 4.26 LGRR372 pKa = 11.84 FAPTFMAGLLRR383 pKa = 11.84 IFGLQTSSQVAEE395 pKa = 3.83 AHH397 pKa = 5.93 FCTVGGMYY405 pKa = 10.6 LSEE408 pKa = 4.15 NVDD411 pKa = 2.59 RR412 pKa = 11.84 HH413 pKa = 5.7 LRR415 pKa = 11.84 HH416 pKa = 5.82 KK417 pKa = 9.35 TVAPYY422 pKa = 9.94 FWVEE426 pKa = 3.75 PTSLIEE432 pKa = 4.04 VGFLGSTAEE441 pKa = 4.12 TAGFGSLVTPGDD453 pKa = 3.21 QAMIPTFEE461 pKa = 4.75 RR462 pKa = 11.84 VRR464 pKa = 11.84 EE465 pKa = 3.88 MDD467 pKa = 2.88 RR468 pKa = 11.84 GRR470 pKa = 11.84 NANFSTIAFKK480 pKa = 10.48 MRR482 pKa = 11.84 TARR485 pKa = 11.84 TSGLVCAHH493 pKa = 7.18 AAAPTPLAGLKK504 pKa = 10.11 LYY506 pKa = 10.81 QFDD509 pKa = 3.83 QDD511 pKa = 4.16 SLILAGDD518 pKa = 3.59 QGPTNGDD525 pKa = 3.27 VPTKK529 pKa = 10.42 HH530 pKa = 5.96 VAADD534 pKa = 3.83 PLSSYY539 pKa = 10.76 LWVRR543 pKa = 11.84 GQSAIPAPAEE553 pKa = 4.06 MINTQASYY561 pKa = 9.88 AAKK564 pKa = 9.99 YY565 pKa = 10.59 KK566 pKa = 10.86 NITWDD571 pKa = 3.54 DD572 pKa = 3.84 DD573 pKa = 4.34 FEE575 pKa = 4.69 GTVSDD580 pKa = 5.69 LPKK583 pKa = 10.44 AWEE586 pKa = 4.33 LEE588 pKa = 4.02 HH589 pKa = 6.6 DD590 pKa = 3.99 TMWRR594 pKa = 11.84 VTVPTAFSVTGPSNYY609 pKa = 9.69 LDD611 pKa = 3.53 RR612 pKa = 11.84 EE613 pKa = 4.09 ARR615 pKa = 11.84 RR616 pKa = 11.84 ARR618 pKa = 11.84 SRR620 pKa = 11.84 AAISLAQAALRR631 pKa = 11.84 ARR633 pKa = 11.84 AYY635 pKa = 10.5 GEE637 pKa = 4.19 ANSPVIDD644 pKa = 4.03 VSNVPPTWDD653 pKa = 3.5 DD654 pKa = 2.86 EE655 pKa = 4.37 RR656 pKa = 11.84 APVVVFSDD664 pKa = 4.98 EE665 pKa = 3.89 IHH667 pKa = 6.27 TSDD670 pKa = 4.47 PGTAQGRR677 pKa = 11.84 GIGNPPDD684 pKa = 3.15 VTAPVTGAPLVPIPHH699 pKa = 6.42 HH700 pKa = 5.9 QPLRR704 pKa = 11.84 GPPFPRR710 pKa = 11.84 GAGVMGGGVAPPPPAGPGGPPAGPGPNPPPPPPPGGDD747 pKa = 3.36 GGDD750 pKa = 3.8 DD751 pKa = 3.26 AAAAAAVAGGDD762 pKa = 3.43 VHH764 pKa = 6.68 GAAGALPAPQVV775 pKa = 3.24
Molecular weight: 80.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.67
IPC2_protein 5.525
IPC_protein 5.588
Toseland 6.059
ProMoST 5.944
Dawson 5.842
Bjellqvist 5.855
Wikipedia 5.855
Rodwell 5.83
Grimsley 6.249
Solomon 5.842
Lehninger 5.83
Nozaki 6.109
DTASelect 6.313
Thurlkill 6.3
EMBOSS 6.262
Sillero 6.198
Patrickios 4.177
IPC_peptide 5.855
IPC2_peptide 6.224
IPC2.peptide.svr19 6.111
Protein with the highest isoelectric point:
>tr|Q8B5B7|Q8B5B7_9VIRU RNA-directed RNA polymerase OS=Coniothyrium minitans RNA virus OX=210827 PE=3 SV=1
MM1 pKa = 7.21 IGARR5 pKa = 11.84 VDD7 pKa = 3.69 EE8 pKa = 4.53 RR9 pKa = 11.84 ALAAGALGQYY19 pKa = 9.45 LKK21 pKa = 11.04 KK22 pKa = 9.85 LTRR25 pKa = 11.84 SQDD28 pKa = 3.1 VSRR31 pKa = 11.84 FATLSFPDD39 pKa = 3.39 QISYY43 pKa = 9.97 VFKK46 pKa = 10.53 PSWDD50 pKa = 3.52 SFRR53 pKa = 11.84 PTPLHH58 pKa = 6.14 RR59 pKa = 11.84 AALSFLCGEE68 pKa = 4.48 VPVQVALPYY77 pKa = 9.98 ASLWRR82 pKa = 11.84 MVDD85 pKa = 3.28 YY86 pKa = 8.78 TCPVPDD92 pKa = 3.26 IVLDD96 pKa = 3.85 IKK98 pKa = 11.44 YY99 pKa = 6.82 KK100 pKa = 9.22 TRR102 pKa = 11.84 WIRR105 pKa = 11.84 DD106 pKa = 2.95 AGITVKK112 pKa = 10.57 RR113 pKa = 11.84 FPLKK117 pKa = 10.58 KK118 pKa = 10.47 NPAASNKK125 pKa = 9.07 VNLYY129 pKa = 8.52 LHH131 pKa = 6.41 EE132 pKa = 4.45 VGRR135 pKa = 11.84 DD136 pKa = 3.75 LSSHH140 pKa = 6.81 APTQLNVGLQYY151 pKa = 10.99 LDD153 pKa = 2.95 WVRR156 pKa = 11.84 RR157 pKa = 11.84 QRR159 pKa = 11.84 VVYY162 pKa = 10.23 NDD164 pKa = 2.99 QATAFLLYY172 pKa = 9.36 EE173 pKa = 4.17 YY174 pKa = 10.74 CLACYY179 pKa = 10.03 DD180 pKa = 3.76 SRR182 pKa = 11.84 GWRR185 pKa = 11.84 HH186 pKa = 5.41 AAASLFDD193 pKa = 4.01 SDD195 pKa = 4.22 YY196 pKa = 11.69 AKK198 pKa = 10.82 GFSVFGKK205 pKa = 10.33 AVGINGSQVGAMMVEE220 pKa = 4.42 TNVLLGRR227 pKa = 11.84 DD228 pKa = 3.78 VAPIDD233 pKa = 3.77 LVEE236 pKa = 4.32 EE237 pKa = 4.04 ARR239 pKa = 11.84 KK240 pKa = 7.51 RR241 pKa = 11.84 TSMRR245 pKa = 11.84 EE246 pKa = 3.75 VRR248 pKa = 11.84 AMTTAYY254 pKa = 10.76 SDD256 pKa = 3.35 DD257 pKa = 3.93 TIRR260 pKa = 11.84 RR261 pKa = 11.84 AVRR264 pKa = 11.84 TVLLRR269 pKa = 11.84 EE270 pKa = 4.67 LKK272 pKa = 10.55 RR273 pKa = 11.84 SGDD276 pKa = 3.74 SYY278 pKa = 11.3 ILEE281 pKa = 4.49 FPTLEE286 pKa = 4.18 DD287 pKa = 2.61 HH288 pKa = 7.29 WATRR292 pKa = 11.84 WQWAVNGAHH301 pKa = 6.62 SGLIYY306 pKa = 9.33 KK307 pKa = 7.59 TNPSYY312 pKa = 10.55 RR313 pKa = 11.84 PHH315 pKa = 6.25 MPGFDD320 pKa = 3.4 RR321 pKa = 11.84 LHH323 pKa = 5.75 RR324 pKa = 11.84 RR325 pKa = 11.84 AWLEE329 pKa = 4.08 TISEE333 pKa = 4.42 DD334 pKa = 5.38 PRR336 pKa = 11.84 PAWDD340 pKa = 2.94 GRR342 pKa = 11.84 TYY344 pKa = 11.29 VSASPKK350 pKa = 10.08 LEE352 pKa = 4.2 HH353 pKa = 6.54 GKK355 pKa = 8.03 TRR357 pKa = 11.84 AIFAWDD363 pKa = 4.4 TINSLAMEE371 pKa = 4.67 HH372 pKa = 6.61 LMSTVEE378 pKa = 4.06 ANWRR382 pKa = 11.84 GEE384 pKa = 3.99 RR385 pKa = 11.84 VILNPGKK392 pKa = 10.51 GGHH395 pKa = 6.87 LGMAQRR401 pKa = 11.84 VQAARR406 pKa = 11.84 NRR408 pKa = 11.84 SGVSLMLDD416 pKa = 3.09 YY417 pKa = 11.57 DD418 pKa = 4.5 DD419 pKa = 6.0 FNSHH423 pKa = 5.25 HH424 pKa = 6.14 TTRR427 pKa = 11.84 TMQIVVEE434 pKa = 4.38 EE435 pKa = 4.39 TCRR438 pKa = 11.84 LTGYY442 pKa = 9.98 PPDD445 pKa = 3.83 LAEE448 pKa = 4.58 KK449 pKa = 10.14 LVSSMEE455 pKa = 3.64 KK456 pKa = 10.23 HH457 pKa = 5.96 YY458 pKa = 10.84 ICVGGAYY465 pKa = 9.42 IGRR468 pKa = 11.84 SKK470 pKa = 9.52 GTLMSGHH477 pKa = 7.24 RR478 pKa = 11.84 LTTYY482 pKa = 10.43 INSVCNEE489 pKa = 3.51 AYY491 pKa = 10.57 LRR493 pKa = 11.84 IEE495 pKa = 4.77 LGDD498 pKa = 4.31 DD499 pKa = 3.65 FLDD502 pKa = 4.16 KK503 pKa = 11.01 NVSLHH508 pKa = 6.41 VGDD511 pKa = 5.06 DD512 pKa = 3.49 VYY514 pKa = 11.55 LGVRR518 pKa = 11.84 SYY520 pKa = 11.46 QEE522 pKa = 3.25 AGYY525 pKa = 8.45 VLRR528 pKa = 11.84 MIRR531 pKa = 11.84 GSKK534 pKa = 10.43 LRR536 pKa = 11.84 MNPAKK541 pKa = 10.37 QSVGHH546 pKa = 5.33 VTTEE550 pKa = 3.91 FLRR553 pKa = 11.84 VASEE557 pKa = 3.78 SRR559 pKa = 11.84 YY560 pKa = 9.91 SYY562 pKa = 11.03 GYY564 pKa = 9.65 LARR567 pKa = 11.84 AVASITSGSWVNEE580 pKa = 3.64 LALAPLEE587 pKa = 4.13 ALTNIVASARR597 pKa = 11.84 SLANRR602 pKa = 11.84 SGIADD607 pKa = 3.57 VALLLVSSTRR617 pKa = 11.84 RR618 pKa = 11.84 MAPLDD623 pKa = 3.75 SRR625 pKa = 11.84 DD626 pKa = 3.64 DD627 pKa = 3.61 TLLRR631 pKa = 11.84 EE632 pKa = 4.2 LLTGKK637 pKa = 9.89 VALQNGPNYY646 pKa = 9.89 QSSGYY651 pKa = 7.94 YY652 pKa = 9.04 RR653 pKa = 11.84 HH654 pKa = 6.12 VAVTPKK660 pKa = 9.69 MVRR663 pKa = 11.84 RR664 pKa = 11.84 DD665 pKa = 3.52 DD666 pKa = 3.62 FGYY669 pKa = 11.04 GVLPLEE675 pKa = 4.5 ATHH678 pKa = 6.98 TYY680 pKa = 10.22 LSSAATVLEE689 pKa = 4.26 IEE691 pKa = 4.59 TLTKK695 pKa = 10.67 AGISVEE701 pKa = 3.77 EE702 pKa = 4.18 DD703 pKa = 2.94 MARR706 pKa = 11.84 ASYY709 pKa = 10.25 KK710 pKa = 10.35 KK711 pKa = 10.1 SAPRR715 pKa = 11.84 DD716 pKa = 3.65 FFSAEE721 pKa = 4.14 CLVTGPVLQRR731 pKa = 11.84 PCVGVEE737 pKa = 3.43 WAEE740 pKa = 3.95 MVLRR744 pKa = 11.84 RR745 pKa = 11.84 PRR747 pKa = 11.84 VTGILSRR754 pKa = 11.84 YY755 pKa = 8.67 PLLLLARR762 pKa = 11.84 YY763 pKa = 8.1 RR764 pKa = 11.84 LPEE767 pKa = 3.91 RR768 pKa = 11.84 VVRR771 pKa = 11.84 EE772 pKa = 3.94 ALAAAGGDD780 pKa = 3.88 YY781 pKa = 7.67 NTPYY785 pKa = 10.73 LDD787 pKa = 3.26 YY788 pKa = 10.94 DD789 pKa = 3.11 AWGEE793 pKa = 4.05 YY794 pKa = 10.72 AHH796 pKa = 6.88 GCVIDD801 pKa = 3.86 TVMSYY806 pKa = 10.55 TDD808 pKa = 3.85 ASALGAKK815 pKa = 8.08 TAAGVLTSTTRR826 pKa = 11.84 MYY828 pKa = 11.15 VV829 pKa = 2.83
Molecular weight: 92.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.496
IPC2_protein 8.507
IPC_protein 8.448
Toseland 8.653
ProMoST 8.814
Dawson 9.121
Bjellqvist 9.107
Wikipedia 9.326
Rodwell 9.18
Grimsley 9.151
Solomon 9.18
Lehninger 9.121
Nozaki 9.077
DTASelect 8.99
Thurlkill 9.004
EMBOSS 9.224
Sillero 9.209
Patrickios 4.317
IPC_peptide 9.165
IPC2_peptide 8.009
IPC2.peptide.svr19 7.805
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1604
775
829
802.0
86.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.282 ± 1.689
0.935 ± 0.192
5.611 ± 0.044
4.177 ± 0.458
2.805 ± 0.363
8.666 ± 1.35
2.494 ± 0.057
3.803 ± 0.301
2.431 ± 0.754
8.479 ± 1.339
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.12 ± 0.377
3.055 ± 0.113
6.297 ± 1.466
2.431 ± 0.184
7.481 ± 0.85
6.983 ± 0.436
6.359 ± 0.061
8.292 ± 0.022
1.434 ± 0.265
3.865 ± 0.934
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here