Paenibacillus phage BN12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sitaravirus; unclassified Sitaravirus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I7SCF4|A0A2I7SCF4_9CAUD Putative site-specific recombinase OS=Paenibacillus phage BN12 OX=2070189 GN=BN12_29 PE=4 SV=1
MM1 pKa = 7.48TEE3 pKa = 4.04FGAVVVVEE11 pKa = 4.15GKK13 pKa = 9.23EE14 pKa = 4.08FKK16 pKa = 10.22LTGDD20 pKa = 3.25ADD22 pKa = 3.58FTNRR26 pKa = 11.84VLGGWYY32 pKa = 9.24TDD34 pKa = 4.14FNDD37 pKa = 3.41ASEE40 pKa = 4.52GEE42 pKa = 4.38EE43 pKa = 4.1YY44 pKa = 10.25QFEE47 pKa = 4.29MSAPGLDD54 pKa = 3.45NEE56 pKa = 4.6GNEE59 pKa = 4.09VTVYY63 pKa = 10.31WIFTDD68 pKa = 3.52IKK70 pKa = 10.28GEE72 pKa = 4.0KK73 pKa = 9.89GKK75 pKa = 10.9EE76 pKa = 3.88SLDD79 pKa = 3.42EE80 pKa = 3.95YY81 pKa = 11.24DD82 pKa = 3.71YY83 pKa = 11.78DD84 pKa = 3.82NVDD87 pKa = 3.34RR88 pKa = 11.84VVYY91 pKa = 9.52EE92 pKa = 3.86

Molecular weight: 10.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I7SCF8|A0A2I7SCF8_9CAUD Putative tail protein OS=Paenibacillus phage BN12 OX=2070189 GN=BN12_16 PE=4 SV=1
MM1 pKa = 7.34GKK3 pKa = 9.83YY4 pKa = 9.57VGSVVEE10 pKa = 5.24IIYY13 pKa = 10.5LDD15 pKa = 3.76RR16 pKa = 11.84NNQITQRR23 pKa = 11.84RR24 pKa = 11.84VRR26 pKa = 11.84VRR28 pKa = 11.84SIKK31 pKa = 10.86GNILQAYY38 pKa = 8.05CFLAKK43 pKa = 10.03SPRR46 pKa = 11.84VFKK49 pKa = 11.11LEE51 pKa = 4.27NILAINAVNRR61 pKa = 11.84HH62 pKa = 4.43VVV64 pKa = 2.9

Molecular weight: 7.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

12732

34

878

174.4

19.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.637 ± 0.385

1.045 ± 0.159

5.616 ± 0.225

8.145 ± 0.378

3.613 ± 0.204

6.166 ± 0.358

1.799 ± 0.166

6.927 ± 0.311

8.475 ± 0.337

8.577 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.118 ± 0.184

4.548 ± 0.199

3.385 ± 0.214

4.045 ± 0.205

5.137 ± 0.378

6.087 ± 0.315

5.514 ± 0.212

6.181 ± 0.25

1.453 ± 0.166

3.534 ± 0.218
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski