Amino acid dipepetide frequency for Paenibacillus phage BN12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.263AlaAla: 5.263 ± 0.913
0.471AlaCys: 0.471 ± 0.154
4.32AlaAsp: 4.32 ± 0.639
6.284AlaGlu: 6.284 ± 0.78
1.65AlaPhe: 1.65 ± 0.3
4.791AlaGly: 4.791 ± 0.8
0.707AlaHis: 0.707 ± 0.238
4.556AlaIle: 4.556 ± 0.844
5.341AlaLys: 5.341 ± 0.72
6.048AlaLeu: 6.048 ± 0.646
1.492AlaMet: 1.492 ± 0.305
3.063AlaAsn: 3.063 ± 0.457
1.65AlaPro: 1.65 ± 0.319
2.042AlaGln: 2.042 ± 0.422
2.199AlaArg: 2.199 ± 0.396
4.163AlaSer: 4.163 ± 0.562
4.085AlaThr: 4.085 ± 0.701
4.085AlaVal: 4.085 ± 0.526
1.492AlaTrp: 1.492 ± 0.401
2.671AlaTyr: 2.671 ± 0.397
0.0AlaXaa: 0.0 ± 0.0
Cys
0.628CysAla: 0.628 ± 0.217
0.236CysCys: 0.236 ± 0.15
0.314CysAsp: 0.314 ± 0.189
0.707CysGlu: 0.707 ± 0.223
0.393CysPhe: 0.393 ± 0.16
0.943CysGly: 0.943 ± 0.315
0.236CysHis: 0.236 ± 0.147
0.55CysIle: 0.55 ± 0.179
1.257CysLys: 1.257 ± 0.409
0.864CysLeu: 0.864 ± 0.307
0.314CysMet: 0.314 ± 0.147
0.236CysAsn: 0.236 ± 0.143
0.628CysPro: 0.628 ± 0.26
0.393CysGln: 0.393 ± 0.191
0.628CysArg: 0.628 ± 0.232
0.393CysSer: 0.393 ± 0.143
0.471CysThr: 0.471 ± 0.155
0.707CysVal: 0.707 ± 0.281
0.236CysTrp: 0.236 ± 0.125
0.314CysTyr: 0.314 ± 0.147
0.0CysXaa: 0.0 ± 0.0
Asp
4.242AspAla: 4.242 ± 0.705
0.628AspCys: 0.628 ± 0.246
3.77AspAsp: 3.77 ± 0.639
3.849AspGlu: 3.849 ± 0.641
3.378AspPhe: 3.378 ± 0.513
3.613AspGly: 3.613 ± 0.497
0.785AspHis: 0.785 ± 0.239
3.692AspIle: 3.692 ± 0.468
2.906AspLys: 2.906 ± 0.479
5.498AspLeu: 5.498 ± 0.676
1.964AspMet: 1.964 ± 0.378
1.728AspAsn: 1.728 ± 0.348
2.042AspPro: 2.042 ± 0.33
1.65AspGln: 1.65 ± 0.413
3.456AspArg: 3.456 ± 0.632
2.985AspSer: 2.985 ± 0.462
3.456AspThr: 3.456 ± 0.442
3.927AspVal: 3.927 ± 0.496
0.785AspTrp: 0.785 ± 0.263
1.807AspTyr: 1.807 ± 0.373
0.0AspXaa: 0.0 ± 0.0
Glu
4.949GluAla: 4.949 ± 0.662
0.55GluCys: 0.55 ± 0.175
5.106GluAsp: 5.106 ± 0.833
5.577GluGlu: 5.577 ± 0.754
2.749GluPhe: 2.749 ± 0.395
3.849GluGly: 3.849 ± 0.506
1.414GluHis: 1.414 ± 0.481
5.891GluIle: 5.891 ± 0.778
7.226GluLys: 7.226 ± 0.755
6.205GluLeu: 6.205 ± 0.676
4.32GluMet: 4.32 ± 0.578
3.456GluAsn: 3.456 ± 0.567
2.199GluPro: 2.199 ± 0.39
4.085GluGln: 4.085 ± 0.724
4.87GluArg: 4.87 ± 0.733
4.556GluSer: 4.556 ± 0.569
4.713GluThr: 4.713 ± 0.612
5.498GluVal: 5.498 ± 0.728
1.1GluTrp: 1.1 ± 0.311
3.142GluTyr: 3.142 ± 0.513
0.0GluXaa: 0.0 ± 0.0
Phe
1.65PheAla: 1.65 ± 0.419
0.707PheCys: 0.707 ± 0.166
2.199PheAsp: 2.199 ± 0.432
3.22PheGlu: 3.22 ± 0.526
1.178PhePhe: 1.178 ± 0.288
2.514PheGly: 2.514 ± 0.519
1.1PheHis: 1.1 ± 0.333
3.063PheIle: 3.063 ± 0.462
2.592PheLys: 2.592 ± 0.59
2.906PheLeu: 2.906 ± 0.394
0.943PheMet: 0.943 ± 0.254
1.964PheAsn: 1.964 ± 0.373
1.335PhePro: 1.335 ± 0.302
1.178PheGln: 1.178 ± 0.338
0.943PheArg: 0.943 ± 0.28
2.749PheSer: 2.749 ± 0.562
1.807PheThr: 1.807 ± 0.369
2.121PheVal: 2.121 ± 0.479
0.471PheTrp: 0.471 ± 0.164
1.492PheTyr: 1.492 ± 0.361
0.0PheXaa: 0.0 ± 0.0
Gly
3.456GlyAla: 3.456 ± 0.568
0.55GlyCys: 0.55 ± 0.19
3.142GlyAsp: 3.142 ± 0.592
4.87GlyGlu: 4.87 ± 0.689
2.199GlyPhe: 2.199 ± 0.423
3.299GlyGly: 3.299 ± 0.589
0.864GlyHis: 0.864 ± 0.256
5.577GlyIle: 5.577 ± 1.034
7.226GlyLys: 7.226 ± 0.608
5.184GlyLeu: 5.184 ± 0.865
1.65GlyMet: 1.65 ± 0.591
3.22GlyAsn: 3.22 ± 0.452
0.864GlyPro: 0.864 ± 0.299
2.356GlyGln: 2.356 ± 0.537
2.985GlyArg: 2.985 ± 0.517
3.849GlySer: 3.849 ± 0.675
2.985GlyThr: 2.985 ± 0.59
4.163GlyVal: 4.163 ± 0.591
1.021GlyTrp: 1.021 ± 0.235
2.199GlyTyr: 2.199 ± 0.507
0.0GlyXaa: 0.0 ± 0.0
His
1.335HisAla: 1.335 ± 0.285
0.236HisCys: 0.236 ± 0.136
0.471HisAsp: 0.471 ± 0.195
1.021HisGlu: 1.021 ± 0.296
1.021HisPhe: 1.021 ± 0.339
0.707HisGly: 0.707 ± 0.269
0.314HisHis: 0.314 ± 0.172
1.964HisIle: 1.964 ± 0.429
1.1HisLys: 1.1 ± 0.316
2.278HisLeu: 2.278 ± 0.425
0.393HisMet: 0.393 ± 0.154
1.335HisAsn: 1.335 ± 0.308
0.707HisPro: 0.707 ± 0.217
0.55HisGln: 0.55 ± 0.198
1.021HisArg: 1.021 ± 0.257
1.1HisSer: 1.1 ± 0.3
0.628HisThr: 0.628 ± 0.198
1.178HisVal: 1.178 ± 0.298
0.236HisTrp: 0.236 ± 0.127
0.393HisTyr: 0.393 ± 0.189
0.0HisXaa: 0.0 ± 0.0
Ile
3.535IleAla: 3.535 ± 0.609
0.943IleCys: 0.943 ± 0.275
4.006IleAsp: 4.006 ± 0.64
6.441IleGlu: 6.441 ± 0.699
3.142IlePhe: 3.142 ± 0.421
4.163IleGly: 4.163 ± 0.583
1.728IleHis: 1.728 ± 0.359
3.299IleIle: 3.299 ± 0.729
6.52IleLys: 6.52 ± 1.102
5.734IleLeu: 5.734 ± 0.676
1.65IleMet: 1.65 ± 0.394
3.378IleAsn: 3.378 ± 0.485
2.514IlePro: 2.514 ± 0.368
2.828IleGln: 2.828 ± 0.531
4.242IleArg: 4.242 ± 0.644
3.299IleSer: 3.299 ± 0.55
3.927IleThr: 3.927 ± 0.412
4.634IleVal: 4.634 ± 0.767
0.707IleTrp: 0.707 ± 0.294
2.592IleTyr: 2.592 ± 0.437
0.0IleXaa: 0.0 ± 0.0
Lys
6.912LysAla: 6.912 ± 0.641
0.628LysCys: 0.628 ± 0.229
3.927LysAsp: 3.927 ± 0.456
9.033LysGlu: 9.033 ± 1.053
2.121LysPhe: 2.121 ± 0.3
5.184LysGly: 5.184 ± 0.527
1.65LysHis: 1.65 ± 0.274
5.42LysIle: 5.42 ± 0.608
6.677LysLys: 6.677 ± 0.968
7.069LysLeu: 7.069 ± 0.701
3.299LysMet: 3.299 ± 0.502
3.927LysAsn: 3.927 ± 0.572
2.356LysPro: 2.356 ± 0.432
4.006LysGln: 4.006 ± 0.563
4.713LysArg: 4.713 ± 0.642
5.577LysSer: 5.577 ± 0.777
4.634LysThr: 4.634 ± 0.803
3.77LysVal: 3.77 ± 0.587
1.1LysTrp: 1.1 ± 0.247
2.671LysTyr: 2.671 ± 0.418
0.0LysXaa: 0.0 ± 0.0
Leu
6.598LeuAla: 6.598 ± 0.729
1.021LeuCys: 1.021 ± 0.345
5.027LeuAsp: 5.027 ± 0.561
6.991LeuGlu: 6.991 ± 0.695
2.749LeuPhe: 2.749 ± 0.583
5.498LeuGly: 5.498 ± 0.639
1.885LeuHis: 1.885 ± 0.362
4.713LeuIle: 4.713 ± 0.483
7.305LeuLys: 7.305 ± 0.612
6.362LeuLeu: 6.362 ± 0.842
2.278LeuMet: 2.278 ± 0.438
4.32LeuAsn: 4.32 ± 0.486
2.985LeuPro: 2.985 ± 0.493
4.32LeuGln: 4.32 ± 0.543
3.849LeuArg: 3.849 ± 0.539
5.891LeuSer: 5.891 ± 0.584
5.655LeuThr: 5.655 ± 0.736
4.242LeuVal: 4.242 ± 0.58
1.257LeuTrp: 1.257 ± 0.326
2.828LeuTyr: 2.828 ± 0.488
0.0LeuXaa: 0.0 ± 0.0
Met
2.121MetAla: 2.121 ± 0.404
0.079MetCys: 0.079 ± 0.081
1.492MetAsp: 1.492 ± 0.295
2.121MetGlu: 2.121 ± 0.368
1.257MetPhe: 1.257 ± 0.3
1.414MetGly: 1.414 ± 0.34
0.079MetHis: 0.079 ± 0.084
2.278MetIle: 2.278 ± 0.487
3.613MetLys: 3.613 ± 0.601
3.142MetLeu: 3.142 ± 0.574
1.335MetMet: 1.335 ± 0.387
2.671MetAsn: 2.671 ± 0.478
1.178MetPro: 1.178 ± 0.272
1.414MetGln: 1.414 ± 0.257
1.335MetArg: 1.335 ± 0.354
2.199MetSer: 2.199 ± 0.388
1.335MetThr: 1.335 ± 0.316
0.864MetVal: 0.864 ± 0.245
0.157MetTrp: 0.157 ± 0.1
1.1MetTyr: 1.1 ± 0.305
0.0MetXaa: 0.0 ± 0.0
Asn
2.278AsnAla: 2.278 ± 0.342
0.471AsnCys: 0.471 ± 0.168
2.356AsnAsp: 2.356 ± 0.476
3.692AsnGlu: 3.692 ± 0.554
1.1AsnPhe: 1.1 ± 0.282
4.006AsnGly: 4.006 ± 0.576
1.021AsnHis: 1.021 ± 0.261
2.985AsnIle: 2.985 ± 0.477
3.613AsnLys: 3.613 ± 0.607
3.613AsnLeu: 3.613 ± 0.537
1.257AsnMet: 1.257 ± 0.45
1.964AsnAsn: 1.964 ± 0.451
2.278AsnPro: 2.278 ± 0.479
1.807AsnGln: 1.807 ± 0.427
3.849AsnArg: 3.849 ± 0.63
2.514AsnSer: 2.514 ± 0.451
2.356AsnThr: 2.356 ± 0.383
2.356AsnVal: 2.356 ± 0.55
0.785AsnTrp: 0.785 ± 0.245
1.178AsnTyr: 1.178 ± 0.273
0.0AsnXaa: 0.0 ± 0.0
Pro
2.278ProAla: 2.278 ± 0.387
0.157ProCys: 0.157 ± 0.109
1.335ProAsp: 1.335 ± 0.268
3.142ProGlu: 3.142 ± 0.672
1.257ProPhe: 1.257 ± 0.28
2.435ProGly: 2.435 ± 0.459
0.943ProHis: 0.943 ± 0.237
2.906ProIle: 2.906 ± 0.421
3.063ProLys: 3.063 ± 0.551
2.514ProLeu: 2.514 ± 0.408
0.864ProMet: 0.864 ± 0.247
1.728ProAsn: 1.728 ± 0.362
1.1ProPro: 1.1 ± 0.29
0.864ProGln: 0.864 ± 0.288
0.943ProArg: 0.943 ± 0.216
2.985ProSer: 2.985 ± 0.591
1.728ProThr: 1.728 ± 0.367
2.042ProVal: 2.042 ± 0.541
0.471ProTrp: 0.471 ± 0.228
1.1ProTyr: 1.1 ± 0.263
0.0ProXaa: 0.0 ± 0.0
Gln
3.456GlnAla: 3.456 ± 0.57
0.236GlnCys: 0.236 ± 0.125
2.121GlnAsp: 2.121 ± 0.329
3.456GlnGlu: 3.456 ± 0.561
1.65GlnPhe: 1.65 ± 0.311
2.671GlnGly: 2.671 ± 0.54
0.864GlnHis: 0.864 ± 0.233
2.199GlnIle: 2.199 ± 0.402
2.985GlnLys: 2.985 ± 0.553
4.085GlnLeu: 4.085 ± 0.75
1.807GlnMet: 1.807 ± 0.389
1.1GlnAsn: 1.1 ± 0.24
1.885GlnPro: 1.885 ± 0.499
1.728GlnGln: 1.728 ± 0.515
2.278GlnArg: 2.278 ± 0.434
2.199GlnSer: 2.199 ± 0.437
2.356GlnThr: 2.356 ± 0.377
1.885GlnVal: 1.885 ± 0.459
0.393GlnTrp: 0.393 ± 0.211
1.1GlnTyr: 1.1 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
2.906ArgAla: 2.906 ± 0.532
0.55ArgCys: 0.55 ± 0.229
2.592ArgAsp: 2.592 ± 0.454
4.006ArgGlu: 4.006 ± 0.608
1.65ArgPhe: 1.65 ± 0.378
2.671ArgGly: 2.671 ± 0.559
1.1ArgHis: 1.1 ± 0.264
3.299ArgIle: 3.299 ± 0.547
6.205ArgLys: 6.205 ± 0.935
4.556ArgLeu: 4.556 ± 0.625
1.65ArgMet: 1.65 ± 0.374
2.278ArgAsn: 2.278 ± 0.472
1.571ArgPro: 1.571 ± 0.37
2.356ArgGln: 2.356 ± 0.41
2.985ArgArg: 2.985 ± 0.533
2.199ArgSer: 2.199 ± 0.435
2.435ArgThr: 2.435 ± 0.442
3.299ArgVal: 3.299 ± 0.499
0.707ArgTrp: 0.707 ± 0.26
2.356ArgTyr: 2.356 ± 0.391
0.0ArgXaa: 0.0 ± 0.0
Ser
5.263SerAla: 5.263 ± 0.956
0.471SerCys: 0.471 ± 0.216
3.849SerAsp: 3.849 ± 0.496
4.556SerGlu: 4.556 ± 0.578
2.356SerPhe: 2.356 ± 0.472
3.849SerGly: 3.849 ± 0.623
1.335SerHis: 1.335 ± 0.369
6.048SerIle: 6.048 ± 0.778
3.692SerLys: 3.692 ± 0.598
5.184SerLeu: 5.184 ± 0.686
1.492SerMet: 1.492 ± 0.329
1.807SerAsn: 1.807 ± 0.349
2.121SerPro: 2.121 ± 0.339
2.435SerGln: 2.435 ± 0.549
2.592SerArg: 2.592 ± 0.419
3.613SerSer: 3.613 ± 0.681
3.142SerThr: 3.142 ± 0.491
4.399SerVal: 4.399 ± 0.782
0.707SerTrp: 0.707 ± 0.216
1.964SerTyr: 1.964 ± 0.294
0.0SerXaa: 0.0 ± 0.0
Thr
3.927ThrAla: 3.927 ± 0.661
0.55ThrCys: 0.55 ± 0.277
2.985ThrAsp: 2.985 ± 0.495
4.006ThrGlu: 4.006 ± 0.634
1.65ThrPhe: 1.65 ± 0.358
3.927ThrGly: 3.927 ± 0.554
0.943ThrHis: 0.943 ± 0.302
3.927ThrIle: 3.927 ± 0.568
4.242ThrLys: 4.242 ± 0.514
4.713ThrLeu: 4.713 ± 0.727
1.65ThrMet: 1.65 ± 0.351
1.885ThrAsn: 1.885 ± 0.384
2.985ThrPro: 2.985 ± 0.509
2.278ThrGln: 2.278 ± 0.384
2.356ThrArg: 2.356 ± 0.489
3.299ThrSer: 3.299 ± 0.51
2.514ThrThr: 2.514 ± 0.475
4.713ThrVal: 4.713 ± 0.551
0.707ThrTrp: 0.707 ± 0.227
1.885ThrTyr: 1.885 ± 0.39
0.0ThrXaa: 0.0 ± 0.0
Val
3.299ValAla: 3.299 ± 0.562
1.1ValCys: 1.1 ± 0.335
3.77ValAsp: 3.77 ± 0.556
4.634ValGlu: 4.634 ± 0.587
2.906ValPhe: 2.906 ± 0.465
3.142ValGly: 3.142 ± 0.605
0.471ValHis: 0.471 ± 0.34
3.77ValIle: 3.77 ± 0.604
5.341ValLys: 5.341 ± 0.62
5.106ValLeu: 5.106 ± 0.596
1.65ValMet: 1.65 ± 0.344
2.749ValAsn: 2.749 ± 0.528
1.807ValPro: 1.807 ± 0.332
1.885ValGln: 1.885 ± 0.379
3.142ValArg: 3.142 ± 0.58
4.32ValSer: 4.32 ± 0.505
4.399ValThr: 4.399 ± 0.512
4.242ValVal: 4.242 ± 0.589
1.65ValTrp: 1.65 ± 0.939
2.435ValTyr: 2.435 ± 0.546
0.0ValXaa: 0.0 ± 0.0
Trp
0.864TrpAla: 0.864 ± 0.242
0.157TrpCys: 0.157 ± 0.11
0.55TrpAsp: 0.55 ± 0.275
0.943TrpGlu: 0.943 ± 0.237
0.707TrpPhe: 0.707 ± 0.209
1.021TrpGly: 1.021 ± 0.256
0.314TrpHis: 0.314 ± 0.161
0.628TrpIle: 0.628 ± 0.205
1.1TrpLys: 1.1 ± 0.313
1.728TrpLeu: 1.728 ± 0.365
0.236TrpMet: 0.236 ± 0.143
1.571TrpAsn: 1.571 ± 1.072
0.314TrpPro: 0.314 ± 0.246
0.785TrpGln: 0.785 ± 0.261
0.628TrpArg: 0.628 ± 0.214
0.943TrpSer: 0.943 ± 0.248
0.55TrpThr: 0.55 ± 0.205
0.707TrpVal: 0.707 ± 0.225
0.236TrpTrp: 0.236 ± 0.153
0.55TrpTyr: 0.55 ± 0.196
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.414TyrAla: 1.414 ± 0.364
0.707TyrCys: 0.707 ± 0.255
2.828TyrAsp: 2.828 ± 0.498
2.906TyrGlu: 2.906 ± 0.419
1.021TyrPhe: 1.021 ± 0.24
1.964TyrGly: 1.964 ± 0.419
0.236TyrHis: 0.236 ± 0.117
2.514TyrIle: 2.514 ± 0.425
2.749TyrLys: 2.749 ± 0.382
2.985TyrLeu: 2.985 ± 0.49
0.864TyrMet: 0.864 ± 0.247
1.1TyrAsn: 1.1 ± 0.322
1.335TyrPro: 1.335 ± 0.353
1.492TyrGln: 1.492 ± 0.361
2.356TyrArg: 2.356 ± 0.386
2.042TyrSer: 2.042 ± 0.494
1.964TyrThr: 1.964 ± 0.362
2.985TyrVal: 2.985 ± 0.471
0.314TyrTrp: 0.314 ± 0.15
1.571TyrTyr: 1.571 ± 0.32
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (12732 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski