Okeania hirsuta

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Oscillatoriaceae; Okeania

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6134 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N6PBX9|A0A3N6PBX9_9CYAN DUF1257 domain-containing protein OS=Okeania hirsuta OX=1458930 GN=D5R40_11520 PE=4 SV=1
MM1 pKa = 7.47FSGLFTILTTAANSVSSTTLTKK23 pKa = 10.82GILVAGAEE31 pKa = 4.32DD32 pKa = 4.77CSWLLPATATSFTGRR47 pKa = 11.84TNLTFSNDD55 pKa = 2.93YY56 pKa = 10.88RR57 pKa = 11.84LIFDD61 pKa = 4.32WTAQDD66 pKa = 4.29DD67 pKa = 4.26NSDD70 pKa = 3.82SVILSSEE77 pKa = 3.77LDD79 pKa = 3.08SWYY82 pKa = 11.16VEE84 pKa = 3.97LSSVLGLGVLLWDD97 pKa = 4.23SSDD100 pKa = 3.47SAFASEE106 pKa = 4.01WQANLDD112 pKa = 3.58FLYY115 pKa = 10.82DD116 pKa = 3.58IDD118 pKa = 6.09ADD120 pKa = 4.1DD121 pKa = 4.62VKK123 pKa = 11.19LFTNPYY129 pKa = 7.6TDD131 pKa = 3.36EE132 pKa = 4.38GLSFRR137 pKa = 11.84IIWQLVVEE145 pKa = 4.69LVCMCLLIDD154 pKa = 4.25IQWISQQYY162 pKa = 9.15LCRR165 pKa = 11.84AKK167 pKa = 10.73LL168 pKa = 3.33

Molecular weight: 18.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N6PS79|A0A3N6PS79_9CYAN Crossover junction endodeoxyribonuclease RuvC OS=Okeania hirsuta OX=1458930 GN=ruvC PE=3 SV=1
MM1 pKa = 6.63TQRR4 pKa = 11.84TLHH7 pKa = 4.78GTSRR11 pKa = 11.84KK12 pKa = 8.75RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84VSGFRR20 pKa = 11.84TRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84NGRR29 pKa = 11.84AVIKK33 pKa = 10.42ARR35 pKa = 11.84RR36 pKa = 11.84QKK38 pKa = 10.51GRR40 pKa = 11.84EE41 pKa = 3.7RR42 pKa = 11.84LAVYY46 pKa = 10.09

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6134

0

6134

1773741

17

3234

289.2

32.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.826 ± 0.037

1.013 ± 0.013

4.989 ± 0.036

6.86 ± 0.037

4.217 ± 0.025

6.587 ± 0.048

1.697 ± 0.016

7.5 ± 0.035

5.836 ± 0.036

10.519 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.888 ± 0.018

5.107 ± 0.037

4.394 ± 0.025

4.962 ± 0.028

4.483 ± 0.028

6.58 ± 0.03

5.529 ± 0.035

6.254 ± 0.027

1.445 ± 0.017

3.313 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski