Flavobacterium sp.

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3029 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A519KHE1|A0A519KHE1_FLASP XRE family transcriptional regulator OS=Flavobacterium sp. OX=239 GN=EOO51_01425 PE=4 SV=1
MM1 pKa = 6.96KK2 pKa = 9.71TLRR5 pKa = 11.84TSILAMLAIFAVSCSTDD22 pKa = 3.26DD23 pKa = 3.51VEE25 pKa = 4.86NRR27 pKa = 11.84PIVEE31 pKa = 4.43AGDD34 pKa = 3.78AAVLTAPEE42 pKa = 4.2EE43 pKa = 4.39GNVYY47 pKa = 10.78VLTPEE52 pKa = 4.31NMDD55 pKa = 3.55ALGEE59 pKa = 4.23RR60 pKa = 11.84FVWTAANFGAGVIPNYY76 pKa = 10.38AIEE79 pKa = 4.28IDD81 pKa = 3.55RR82 pKa = 11.84AGDD85 pKa = 3.65NFDD88 pKa = 3.61TPATIGITNGTTQFAASQSVLNTALLAVGAVPYY121 pKa = 10.25EE122 pKa = 4.08SANFEE127 pKa = 4.41VRR129 pKa = 11.84VKK131 pKa = 10.85AYY133 pKa = 10.35VGDD136 pKa = 3.62MFNYY140 pKa = 10.18SNVAEE145 pKa = 4.6MIITPYY151 pKa = 8.05TTEE154 pKa = 3.8TPKK157 pKa = 10.3IYY159 pKa = 10.65AVGNFQAASGYY170 pKa = 10.38GNDD173 pKa = 3.36WTPADD178 pKa = 4.01GVPLEE183 pKa = 4.23SSGFGLTDD191 pKa = 3.68FEE193 pKa = 5.56GYY195 pKa = 11.11VYY197 pKa = 10.45MNVPTPEE204 pKa = 4.12FKK206 pKa = 10.93LLPTNEE212 pKa = 4.13NFDD215 pKa = 4.01GDD217 pKa = 4.04FGDD220 pKa = 4.91DD221 pKa = 3.36GSFAGMLTQSGAGEE235 pKa = 4.09ANIPLSGPGYY245 pKa = 10.57YY246 pKa = 10.25LIKK249 pKa = 10.74ANTGAVTASNPEE261 pKa = 3.6GMTYY265 pKa = 9.15TAQPTAWGIIGSATPTGWDD284 pKa = 3.21SDD286 pKa = 3.36TDD288 pKa = 3.71MTYY291 pKa = 11.28DD292 pKa = 3.76PATKK296 pKa = 9.95KK297 pKa = 7.24WTITLNLIGGQEE309 pKa = 3.77IKK311 pKa = 10.7FRR313 pKa = 11.84ANDD316 pKa = 3.14AWDD319 pKa = 4.3LNFGDD324 pKa = 5.35DD325 pKa = 3.77GANGSLEE332 pKa = 4.14AGGANIAIPTSGSYY346 pKa = 8.89TVTLDD351 pKa = 3.61LSNPRR356 pKa = 11.84QYY358 pKa = 10.72TYY360 pKa = 11.45SVTANN365 pKa = 3.23

Molecular weight: 38.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A519K7U0|A0A519K7U0_FLASP T9SS type A sorting domain-containing protein (Fragment) OS=Flavobacterium sp. OX=239 GN=EOO51_15005 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3029

0

3029

1023528

21

3895

337.9

37.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.568 ± 0.045

0.862 ± 0.018

5.526 ± 0.037

5.961 ± 0.066

5.098 ± 0.033

6.78 ± 0.053

1.764 ± 0.025

7.326 ± 0.04

6.509 ± 0.072

8.861 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.316 ± 0.025

5.706 ± 0.046

3.835 ± 0.038

3.435 ± 0.025

4.125 ± 0.041

6.768 ± 0.055

6.154 ± 0.086

6.546 ± 0.037

1.001 ± 0.018

3.858 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski