Amino acid dipepetide frequency for Flavobacterium sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.691AlaAla: 6.691 ± 0.102	0.745AlaCys: 0.745 ± 0.045	4.31AlaAsp: 4.31 ± 0.076	4.592AlaGlu: 4.592 ± 0.098	3.634AlaPhe: 3.634 ± 0.057	5.529AlaGly: 5.529 ± 0.103	1.201AlaHis: 1.201 ± 0.036	5.674AlaIle: 5.674 ± 0.085	4.467AlaLys: 4.467 ± 0.089	6.864AlaLeu: 6.864 ± 0.087	1.813AlaMet: 1.813 ± 0.043	3.948AlaAsn: 3.948 ± 0.077	2.696AlaPro: 2.696 ± 0.089	2.766AlaGln: 2.766 ± 0.052	2.691AlaArg: 2.691 ± 0.056	4.867AlaSer: 4.867 ± 0.085	4.963AlaThr: 4.963 ± 0.141	5.119AlaVal: 5.119 ± 0.074	0.623AlaTrp: 0.623 ± 0.028	2.494AlaTyr: 2.494 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.679CysAla: 0.679 ± 0.033	0.125CysCys: 0.125 ± 0.011	0.512CysAsp: 0.512 ± 0.025	0.484CysGlu: 0.484 ± 0.021	0.39CysPhe: 0.39 ± 0.018	0.869CysGly: 0.869 ± 0.047	0.201CysHis: 0.201 ± 0.016	0.61CysIle: 0.61 ± 0.026	0.384CysLys: 0.384 ± 0.019	0.656CysLeu: 0.656 ± 0.027	0.143CysMet: 0.143 ± 0.011	0.458CysAsn: 0.458 ± 0.024	0.394CysPro: 0.394 ± 0.026	0.24CysGln: 0.24 ± 0.016	0.323CysArg: 0.323 ± 0.02	0.743CysSer: 0.743 ± 0.037	0.527CysThr: 0.527 ± 0.028	0.5CysVal: 0.5 ± 0.023	0.064CysTrp: 0.064 ± 0.008	0.319CysTyr: 0.319 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	4.251AspAla: 4.251 ± 0.079	0.513AspCys: 0.513 ± 0.027	3.016AspAsp: 3.016 ± 0.056	3.602AspGlu: 3.602 ± 0.063	3.542AspPhe: 3.542 ± 0.067	4.054AspGly: 4.054 ± 0.085	0.919AspHis: 0.919 ± 0.032	3.909AspIle: 3.909 ± 0.066	3.549AspLys: 3.549 ± 0.064	4.998AspLeu: 4.998 ± 0.078	1.175AspMet: 1.175 ± 0.039	3.023AspAsn: 3.023 ± 0.061	2.032AspPro: 2.032 ± 0.05	1.592AspGln: 1.592 ± 0.037	2.285AspArg: 2.285 ± 0.052	3.236AspSer: 3.236 ± 0.062	2.483AspThr: 2.483 ± 0.055	3.642AspVal: 3.642 ± 0.067	0.699AspTrp: 0.699 ± 0.029	2.741AspTyr: 2.741 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	4.292GluAla: 4.292 ± 0.089	0.385GluCys: 0.385 ± 0.018	2.772GluAsp: 2.772 ± 0.06	3.684GluGlu: 3.684 ± 0.093	2.781GluPhe: 2.781 ± 0.066	3.348GluGly: 3.348 ± 0.069	1.065GluHis: 1.065 ± 0.036	5.118GluIle: 5.118 ± 0.074	5.219GluLys: 5.219 ± 0.111	5.43GluLeu: 5.43 ± 0.095	1.687GluMet: 1.687 ± 0.039	3.992GluAsn: 3.992 ± 0.068	1.649GluPro: 1.649 ± 0.049	2.155GluGln: 2.155 ± 0.052	2.636GluArg: 2.636 ± 0.063	3.404GluSer: 3.404 ± 0.063	3.37GluThr: 3.37 ± 0.061	3.914GluVal: 3.914 ± 0.074	0.578GluTrp: 0.578 ± 0.025	2.13GluTyr: 2.13 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	3.714PheAla: 3.714 ± 0.061	0.496PheCys: 0.496 ± 0.023	3.331PheAsp: 3.331 ± 0.06	3.092PheGlu: 3.092 ± 0.069	2.534PhePhe: 2.534 ± 0.062	3.747PheGly: 3.747 ± 0.076	0.836PheHis: 0.836 ± 0.031	3.333PheIle: 3.333 ± 0.074	2.67PheLys: 2.67 ± 0.056	4.389PheLeu: 4.389 ± 0.081	1.01PheMet: 1.01 ± 0.033	3.005PheAsn: 3.005 ± 0.065	1.867PhePro: 1.867 ± 0.04	1.471PheGln: 1.471 ± 0.042	2.314PheArg: 2.314 ± 0.05	4.032PheSer: 4.032 ± 0.072	3.465PheThr: 3.465 ± 0.082	3.2PheVal: 3.2 ± 0.062	0.541PheTrp: 0.541 ± 0.023	1.935PheTyr: 1.935 ± 0.051	0.0PheXaa: 0.0 ± 0.0
Gly	4.815GlyAla: 4.815 ± 0.086	0.715GlyCys: 0.715 ± 0.035	3.454GlyAsp: 3.454 ± 0.074	3.55GlyGlu: 3.55 ± 0.054	3.711GlyPhe: 3.711 ± 0.077	4.839GlyGly: 4.839 ± 0.112	1.203GlyHis: 1.203 ± 0.039	5.453GlyIle: 5.453 ± 0.092	4.93GlyLys: 4.93 ± 0.091	5.533GlyLeu: 5.533 ± 0.089	1.728GlyMet: 1.728 ± 0.049	3.988GlyAsn: 3.988 ± 0.092	1.523GlyPro: 1.523 ± 0.045	2.26GlyGln: 2.26 ± 0.052	2.529GlyArg: 2.529 ± 0.054	4.717GlySer: 4.717 ± 0.102	4.962GlyThr: 4.962 ± 0.141	4.26GlyVal: 4.26 ± 0.071	0.714GlyTrp: 0.714 ± 0.025	2.913GlyTyr: 2.913 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	1.034HisAla: 1.034 ± 0.037	0.185HisCys: 0.185 ± 0.016	0.992HisAsp: 0.992 ± 0.035	1.013HisGlu: 1.013 ± 0.036	1.209HisPhe: 1.209 ± 0.035	1.111HisGly: 1.111 ± 0.034	0.479HisHis: 0.479 ± 0.022	1.216HisIle: 1.216 ± 0.034	0.966HisLys: 0.966 ± 0.028	1.684HisLeu: 1.684 ± 0.048	0.324HisMet: 0.324 ± 0.017	0.996HisAsn: 0.996 ± 0.033	0.896HisPro: 0.896 ± 0.032	0.644HisGln: 0.644 ± 0.027	0.837HisArg: 0.837 ± 0.029	1.096HisSer: 1.096 ± 0.039	0.885HisThr: 0.885 ± 0.032	1.001HisVal: 1.001 ± 0.039	0.22HisTrp: 0.22 ± 0.013	0.855HisTyr: 0.855 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	6.093IleAla: 6.093 ± 0.082	0.703IleCys: 0.703 ± 0.029	4.585IleAsp: 4.585 ± 0.074	4.591IleGlu: 4.591 ± 0.084	3.327IlePhe: 3.327 ± 0.064	4.999IleGly: 4.999 ± 0.087	1.272IleHis: 1.272 ± 0.036	5.005IleIle: 5.005 ± 0.077	4.272IleLys: 4.272 ± 0.077	5.97IleLeu: 5.97 ± 0.101	1.394IleMet: 1.394 ± 0.042	3.96IleAsn: 3.96 ± 0.071	3.182IlePro: 3.182 ± 0.052	2.128IleGln: 2.128 ± 0.043	3.232IleArg: 3.232 ± 0.053	5.634IleSer: 5.634 ± 0.1	4.773IleThr: 4.773 ± 0.092	4.934IleVal: 4.934 ± 0.074	0.649IleTrp: 0.649 ± 0.023	2.552IleTyr: 2.552 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	4.577LysAla: 4.577 ± 0.084	0.384LysCys: 0.384 ± 0.021	3.348LysAsp: 3.348 ± 0.067	4.248LysGlu: 4.248 ± 0.089	2.69LysPhe: 2.69 ± 0.055	3.838LysGly: 3.838 ± 0.069	1.066LysHis: 1.066 ± 0.033	5.38LysIle: 5.38 ± 0.08	5.248LysLys: 5.248 ± 0.102	5.771LysLeu: 5.771 ± 0.102	1.97LysMet: 1.97 ± 0.052	3.928LysAsn: 3.928 ± 0.08	2.49LysPro: 2.49 ± 0.059	2.217LysGln: 2.217 ± 0.054	2.717LysArg: 2.717 ± 0.053	4.201LysSer: 4.201 ± 0.084	3.778LysThr: 3.778 ± 0.077	4.073LysVal: 4.073 ± 0.078	0.618LysTrp: 0.618 ± 0.025	2.552LysTyr: 2.552 ± 0.061	0.0LysXaa: 0.0 ± 0.0
Leu	6.663LeuAla: 6.663 ± 0.095	0.67LeuCys: 0.67 ± 0.026	4.779LeuAsp: 4.779 ± 0.078	5.16LeuGlu: 5.16 ± 0.078	4.421LeuPhe: 4.421 ± 0.081	5.322LeuGly: 5.322 ± 0.088	1.676LeuHis: 1.676 ± 0.045	5.997LeuIle: 5.997 ± 0.097	6.06LeuLys: 6.06 ± 0.098	8.633LeuLeu: 8.633 ± 0.13	2.079LeuMet: 2.079 ± 0.051	4.782LeuAsn: 4.782 ± 0.081	3.852LeuPro: 3.852 ± 0.064	3.391LeuGln: 3.391 ± 0.067	4.018LeuArg: 4.018 ± 0.067	6.721LeuSer: 6.721 ± 0.088	5.253LeuThr: 5.253 ± 0.086	5.419LeuVal: 5.419 ± 0.083	0.74LeuTrp: 0.74 ± 0.031	2.969LeuTyr: 2.969 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	1.879MetAla: 1.879 ± 0.049	0.152MetCys: 0.152 ± 0.012	1.114MetAsp: 1.114 ± 0.04	1.387MetGlu: 1.387 ± 0.036	0.876MetPhe: 0.876 ± 0.034	1.381MetGly: 1.381 ± 0.039	0.423MetHis: 0.423 ± 0.02	1.648MetIle: 1.648 ± 0.044	2.146MetLys: 2.146 ± 0.052	2.106MetLeu: 2.106 ± 0.044	0.615MetMet: 0.615 ± 0.025	1.315MetAsn: 1.315 ± 0.035	1.004MetPro: 1.004 ± 0.032	0.952MetGln: 0.952 ± 0.032	1.106MetArg: 1.106 ± 0.036	1.449MetSer: 1.449 ± 0.038	1.332MetThr: 1.332 ± 0.034	1.413MetVal: 1.413 ± 0.042	0.173MetTrp: 0.173 ± 0.013	0.686MetTyr: 0.686 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	4.168AsnAla: 4.168 ± 0.081	0.522AsnCys: 0.522 ± 0.029	3.077AsnAsp: 3.077 ± 0.067	3.154AsnGlu: 3.154 ± 0.06	3.01AsnPhe: 3.01 ± 0.054	4.39AsnGly: 4.39 ± 0.098	0.982AsnHis: 0.982 ± 0.033	4.049AsnIle: 4.049 ± 0.063	3.116AsnLys: 3.116 ± 0.069	5.008AsnLeu: 5.008 ± 0.07	1.222AsnMet: 1.222 ± 0.036	3.188AsnAsn: 3.188 ± 0.074	2.988AsnPro: 2.988 ± 0.062	1.951AsnGln: 1.951 ± 0.042	2.373AsnArg: 2.373 ± 0.054	3.74AsnSer: 3.74 ± 0.079	3.226AsnThr: 3.226 ± 0.075	3.687AsnVal: 3.687 ± 0.076	0.704AsnTrp: 0.704 ± 0.026	2.504AsnTyr: 2.504 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	3.228ProAla: 3.228 ± 0.097	0.28ProCys: 0.28 ± 0.016	2.433ProAsp: 2.433 ± 0.051	2.965ProGlu: 2.965 ± 0.052	1.993ProPhe: 1.993 ± 0.046	2.575ProGly: 2.575 ± 0.063	0.637ProHis: 0.637 ± 0.027	2.467ProIle: 2.467 ± 0.048	2.208ProLys: 2.208 ± 0.05	3.159ProLeu: 3.159 ± 0.066	0.91ProMet: 0.91 ± 0.03	2.195ProAsn: 2.195 ± 0.061	1.123ProPro: 1.123 ± 0.046	1.452ProGln: 1.452 ± 0.04	1.132ProArg: 1.132 ± 0.039	2.495ProSer: 2.495 ± 0.079	2.344ProThr: 2.344 ± 0.084	3.071ProVal: 3.071 ± 0.064	0.303ProTrp: 0.303 ± 0.021	1.384ProTyr: 1.384 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	2.296GlnAla: 2.296 ± 0.051	0.245GlnCys: 0.245 ± 0.019	1.633GlnAsp: 1.633 ± 0.043	1.869GlnGlu: 1.869 ± 0.046	1.693GlnPhe: 1.693 ± 0.043	1.796GlnGly: 1.796 ± 0.047	0.608GlnHis: 0.608 ± 0.025	2.423GlnIle: 2.423 ± 0.05	2.549GlnLys: 2.549 ± 0.063	3.425GlnLeu: 3.425 ± 0.07	0.929GlnMet: 0.929 ± 0.028	2.161GlnAsn: 2.161 ± 0.055	1.402GlnPro: 1.402 ± 0.043	1.585GlnGln: 1.585 ± 0.043	1.455GlnArg: 1.455 ± 0.037	2.224GlnSer: 2.224 ± 0.051	2.143GlnThr: 2.143 ± 0.056	2.131GlnVal: 2.131 ± 0.046	0.419GlnTrp: 0.419 ± 0.021	1.365GlnTyr: 1.365 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.495ArgAla: 2.495 ± 0.055	0.262ArgCys: 0.262 ± 0.015	2.103ArgAsp: 2.103 ± 0.049	2.882ArgGlu: 2.882 ± 0.061	2.231ArgPhe: 2.231 ± 0.044	2.161ArgGly: 2.161 ± 0.053	0.845ArgHis: 0.845 ± 0.026	3.403ArgIle: 3.403 ± 0.057	3.292ArgLys: 3.292 ± 0.068	3.825ArgLeu: 3.825 ± 0.07	1.204ArgMet: 1.204 ± 0.04	2.585ArgAsn: 2.585 ± 0.056	1.398ArgPro: 1.398 ± 0.04	1.536ArgGln: 1.536 ± 0.045	1.878ArgArg: 1.878 ± 0.05	2.39ArgSer: 2.39 ± 0.048	2.275ArgThr: 2.275 ± 0.054	2.389ArgVal: 2.389 ± 0.057	0.429ArgTrp: 0.429 ± 0.022	1.67ArgTyr: 1.67 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	5.399SerAla: 5.399 ± 0.096	0.701SerCys: 0.701 ± 0.03	3.908SerAsp: 3.908 ± 0.064	3.933SerGlu: 3.933 ± 0.064	3.629SerPhe: 3.629 ± 0.071	5.82SerGly: 5.82 ± 0.135	1.21SerHis: 1.21 ± 0.041	4.612SerIle: 4.612 ± 0.075	3.846SerLys: 3.846 ± 0.071	5.79SerLeu: 5.79 ± 0.075	1.388SerMet: 1.388 ± 0.04	3.592SerAsn: 3.592 ± 0.068	2.572SerPro: 2.572 ± 0.1	2.378SerGln: 2.378 ± 0.054	2.617SerArg: 2.617 ± 0.052	4.331SerSer: 4.331 ± 0.088	3.997SerThr: 3.997 ± 0.093	4.616SerVal: 4.616 ± 0.081	0.705SerTrp: 0.705 ± 0.026	2.636SerTyr: 2.636 ± 0.062	0.0SerXaa: 0.0 ± 0.0
Thr	5.107ThrAla: 5.107 ± 0.136	0.474ThrCys: 0.474 ± 0.025	3.44ThrAsp: 3.44 ± 0.07	3.036ThrGlu: 3.036 ± 0.062	3.208ThrPhe: 3.208 ± 0.078	5.009ThrGly: 5.009 ± 0.111	0.97ThrHis: 0.97 ± 0.034	4.917ThrIle: 4.917 ± 0.108	3.081ThrLys: 3.081 ± 0.069	5.237ThrLeu: 5.237 ± 0.079	1.117ThrMet: 1.117 ± 0.032	3.216ThrAsn: 3.216 ± 0.074	2.934ThrPro: 2.934 ± 0.093	1.885ThrGln: 1.885 ± 0.048	2.015ThrArg: 2.015 ± 0.048	4.022ThrSer: 4.022 ± 0.096	4.203ThrThr: 4.203 ± 0.132	4.649ThrVal: 4.649 ± 0.15	0.654ThrTrp: 0.654 ± 0.032	2.369ThrTyr: 2.369 ± 0.071	0.0ThrXaa: 0.0 ± 0.0
Val	5.207ValAla: 5.207 ± 0.078	0.601ValCys: 0.601 ± 0.028	3.634ValAsp: 3.634 ± 0.067	3.728ValGlu: 3.728 ± 0.074	3.245ValPhe: 3.245 ± 0.064	3.911ValGly: 3.911 ± 0.08	1.043ValHis: 1.043 ± 0.036	4.843ValIle: 4.843 ± 0.08	4.04ValLys: 4.04 ± 0.073	5.706ValLeu: 5.706 ± 0.077	1.424ValMet: 1.424 ± 0.036	3.666ValAsn: 3.666 ± 0.067	2.615ValPro: 2.615 ± 0.055	1.949ValGln: 1.949 ± 0.049	2.811ValArg: 2.811 ± 0.055	4.969ValSer: 4.969 ± 0.09	4.47ValThr: 4.47 ± 0.112	4.629ValVal: 4.629 ± 0.082	0.611ValTrp: 0.611 ± 0.027	2.36ValTyr: 2.36 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.578TrpAla: 0.578 ± 0.028	0.089TrpCys: 0.089 ± 0.01	0.567TrpAsp: 0.567 ± 0.025	0.565TrpGlu: 0.565 ± 0.024	0.552TrpPhe: 0.552 ± 0.028	0.562TrpGly: 0.562 ± 0.025	0.223TrpHis: 0.223 ± 0.013	0.727TrpIle: 0.727 ± 0.03	0.733TrpLys: 0.733 ± 0.027	0.989TrpLeu: 0.989 ± 0.039	0.285TrpMet: 0.285 ± 0.017	0.699TrpAsn: 0.699 ± 0.025	0.241TrpPro: 0.241 ± 0.018	0.439TrpGln: 0.439 ± 0.022	0.415TrpArg: 0.415 ± 0.02	0.632TrpSer: 0.632 ± 0.027	0.66TrpThr: 0.66 ± 0.033	0.544TrpVal: 0.544 ± 0.023	0.114TrpTrp: 0.114 ± 0.011	0.4TrpTyr: 0.4 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.52TyrAla: 2.52 ± 0.052	0.37TyrCys: 0.37 ± 0.021	2.255TyrAsp: 2.255 ± 0.049	2.073TyrGlu: 2.073 ± 0.044	2.31TyrPhe: 2.31 ± 0.054	2.538TyrGly: 2.538 ± 0.058	0.785TyrHis: 0.785 ± 0.024	2.474TyrIle: 2.474 ± 0.051	2.319TyrLys: 2.319 ± 0.05	3.433TyrLeu: 3.433 ± 0.061	0.739TyrMet: 0.739 ± 0.031	2.362TyrAsn: 2.362 ± 0.055	1.496TyrPro: 1.496 ± 0.042	1.36TyrGln: 1.36 ± 0.037	1.867TyrArg: 1.867 ± 0.051	2.776TyrSer: 2.776 ± 0.057	2.432TyrThr: 2.432 ± 0.079	2.271TyrVal: 2.271 ± 0.051	0.455TyrTrp: 0.455 ± 0.025	1.746TyrTyr: 1.746 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3029 proteins (1023528 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---