Microbacterium phage Zepp

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Neferthenavirus; unclassified Neferthenavirus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XJZ8|A0A6G6XJZ8_9CAUD Uncharacterized protein OS=Microbacterium phage Zepp OX=2704041 GN=14 PE=4 SV=1
MM1 pKa = 7.37TVTLYY6 pKa = 10.55DD7 pKa = 3.56AQGTPIAGDD16 pKa = 3.43ATAGQHH22 pKa = 4.9MRR24 pKa = 11.84DD25 pKa = 3.57RR26 pKa = 11.84ADD28 pKa = 3.37EE29 pKa = 4.3LGGYY33 pKa = 9.46IKK35 pKa = 10.74DD36 pKa = 4.12DD37 pKa = 3.54ATGDD41 pKa = 3.6TIYY44 pKa = 10.65PEE46 pKa = 4.27EE47 pKa = 4.33GG48 pKa = 3.06

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XJ77|A0A6G6XJ77_9CAUD Uncharacterized protein OS=Microbacterium phage Zepp OX=2704041 GN=60 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.45LPRR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 10.0AQVVSNTRR15 pKa = 11.84SHH17 pKa = 7.16LGICSNPCTPTRR29 pKa = 11.84DD30 pKa = 3.51ALVV33 pKa = 3.26

Molecular weight: 3.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13031

33

813

210.2

23.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.636 ± 0.464

0.729 ± 0.09

6.308 ± 0.374

6.139 ± 0.428

3.154 ± 0.224

7.812 ± 0.299

1.895 ± 0.183

5.142 ± 0.38

4.942 ± 0.333

8.273 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.318 ± 0.153

3.407 ± 0.203

4.919 ± 0.23

4.274 ± 0.216

5.955 ± 0.372

5.495 ± 0.29

6.508 ± 0.322

7.574 ± 0.315

1.865 ± 0.172

2.655 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski