Amino acid dipepetide frequency for Microbacterium phage Zepp

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.2AlaAla: 13.2 ± 1.461
0.998AlaCys: 0.998 ± 0.319
5.679AlaAsp: 5.679 ± 0.642
5.526AlaGlu: 5.526 ± 0.731
3.914AlaPhe: 3.914 ± 0.507
9.056AlaGly: 9.056 ± 0.896
1.381AlaHis: 1.381 ± 0.312
5.526AlaIle: 5.526 ± 0.846
5.679AlaLys: 5.679 ± 0.807
8.826AlaLeu: 8.826 ± 1.023
2.993AlaMet: 2.993 ± 0.468
3.991AlaAsn: 3.991 ± 0.752
4.835AlaPro: 4.835 ± 0.636
4.988AlaGln: 4.988 ± 0.668
5.909AlaArg: 5.909 ± 0.785
5.065AlaSer: 5.065 ± 0.652
7.368AlaThr: 7.368 ± 0.863
6.293AlaVal: 6.293 ± 0.731
2.072AlaTrp: 2.072 ± 0.491
3.07AlaTyr: 3.07 ± 0.529
0.0AlaXaa: 0.0 ± 0.0
Cys
0.767CysAla: 0.767 ± 0.255
0.077CysCys: 0.077 ± 0.07
0.46CysAsp: 0.46 ± 0.22
0.537CysGlu: 0.537 ± 0.235
0.0CysPhe: 0.0 ± 0.0
0.614CysGly: 0.614 ± 0.192
0.307CysHis: 0.307 ± 0.136
0.384CysIle: 0.384 ± 0.158
0.537CysLys: 0.537 ± 0.201
0.537CysLeu: 0.537 ± 0.2
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.691CysPro: 0.691 ± 0.244
0.153CysGln: 0.153 ± 0.112
0.691CysArg: 0.691 ± 0.174
0.23CysSer: 0.23 ± 0.12
0.46CysThr: 0.46 ± 0.198
0.46CysVal: 0.46 ± 0.179
0.153CysTrp: 0.153 ± 0.108
0.23CysTyr: 0.23 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
5.065AspAla: 5.065 ± 0.761
0.614AspCys: 0.614 ± 0.281
4.988AspAsp: 4.988 ± 0.739
4.375AspGlu: 4.375 ± 1.266
1.995AspPhe: 1.995 ± 0.445
6.063AspGly: 6.063 ± 0.772
0.998AspHis: 0.998 ± 0.216
3.377AspIle: 3.377 ± 0.51
2.763AspLys: 2.763 ± 0.42
5.295AspLeu: 5.295 ± 0.591
1.305AspMet: 1.305 ± 0.267
1.228AspAsn: 1.228 ± 0.307
3.914AspPro: 3.914 ± 0.606
2.84AspGln: 2.84 ± 0.426
4.221AspArg: 4.221 ± 0.538
2.609AspSer: 2.609 ± 0.392
3.684AspThr: 3.684 ± 0.6
4.528AspVal: 4.528 ± 0.518
1.842AspTrp: 1.842 ± 0.406
1.381AspTyr: 1.381 ± 0.349
0.0AspXaa: 0.0 ± 0.0
Glu
6.984GluAla: 6.984 ± 0.741
0.537GluCys: 0.537 ± 0.194
5.372GluAsp: 5.372 ± 0.902
5.372GluGlu: 5.372 ± 1.281
2.072GluPhe: 2.072 ± 0.351
4.528GluGly: 4.528 ± 0.584
1.074GluHis: 1.074 ± 0.259
2.533GluIle: 2.533 ± 0.429
2.916GluLys: 2.916 ± 0.428
6.293GluLeu: 6.293 ± 0.592
1.151GluMet: 1.151 ± 0.319
1.765GluAsn: 1.765 ± 0.339
2.072GluPro: 2.072 ± 0.396
3.3GluGln: 3.3 ± 0.474
2.84GluArg: 2.84 ± 0.513
2.763GluSer: 2.763 ± 0.514
2.916GluThr: 2.916 ± 0.431
4.221GluVal: 4.221 ± 0.608
1.074GluTrp: 1.074 ± 0.285
1.612GluTyr: 1.612 ± 0.297
0.0GluXaa: 0.0 ± 0.0
Phe
2.456PheAla: 2.456 ± 0.457
0.307PheCys: 0.307 ± 0.127
1.535PheAsp: 1.535 ± 0.303
2.149PheGlu: 2.149 ± 0.414
0.767PhePhe: 0.767 ± 0.211
3.147PheGly: 3.147 ± 0.592
0.537PheHis: 0.537 ± 0.233
1.458PheIle: 1.458 ± 0.309
1.919PheLys: 1.919 ± 0.378
2.993PheLeu: 2.993 ± 0.421
0.998PheMet: 0.998 ± 0.287
0.767PheAsn: 0.767 ± 0.268
1.228PhePro: 1.228 ± 0.297
0.844PheGln: 0.844 ± 0.231
1.842PheArg: 1.842 ± 0.405
2.84PheSer: 2.84 ± 0.397
2.533PheThr: 2.533 ± 0.417
2.149PheVal: 2.149 ± 0.344
0.384PheTrp: 0.384 ± 0.157
0.691PheTyr: 0.691 ± 0.226
0.0PheXaa: 0.0 ± 0.0
Gly
6.907GlyAla: 6.907 ± 1.031
0.537GlyCys: 0.537 ± 0.216
4.682GlyAsp: 4.682 ± 0.599
3.991GlyGlu: 3.991 ± 0.612
2.609GlyPhe: 2.609 ± 0.426
5.679GlyGly: 5.679 ± 0.763
1.458GlyHis: 1.458 ± 0.358
5.219GlyIle: 5.219 ± 0.876
4.758GlyLys: 4.758 ± 0.711
6.37GlyLeu: 6.37 ± 0.82
2.379GlyMet: 2.379 ± 0.427
2.609GlyAsn: 2.609 ± 0.488
2.149GlyPro: 2.149 ± 0.432
3.684GlyGln: 3.684 ± 0.657
5.295GlyArg: 5.295 ± 0.684
4.528GlySer: 4.528 ± 0.646
6.447GlyThr: 6.447 ± 0.902
5.219GlyVal: 5.219 ± 0.636
1.151GlyTrp: 1.151 ± 0.278
2.456GlyTyr: 2.456 ± 0.535
0.0GlyXaa: 0.0 ± 0.0
His
1.535HisAla: 1.535 ± 0.293
0.153HisCys: 0.153 ± 0.108
1.535HisAsp: 1.535 ± 0.33
1.151HisGlu: 1.151 ± 0.295
0.691HisPhe: 0.691 ± 0.226
1.919HisGly: 1.919 ± 0.386
0.46HisHis: 0.46 ± 0.233
0.844HisIle: 0.844 ± 0.242
1.074HisLys: 1.074 ± 0.313
1.919HisLeu: 1.919 ± 0.512
0.384HisMet: 0.384 ± 0.169
0.691HisAsn: 0.691 ± 0.301
0.844HisPro: 0.844 ± 0.215
0.921HisGln: 0.921 ± 0.276
0.691HisArg: 0.691 ± 0.225
0.767HisSer: 0.767 ± 0.207
1.228HisThr: 1.228 ± 0.357
1.074HisVal: 1.074 ± 0.279
0.153HisTrp: 0.153 ± 0.116
0.921HisTyr: 0.921 ± 0.246
0.0HisXaa: 0.0 ± 0.0
Ile
5.065IleAla: 5.065 ± 0.513
0.23IleCys: 0.23 ± 0.136
3.607IleAsp: 3.607 ± 0.699
3.837IleGlu: 3.837 ± 0.59
0.767IlePhe: 0.767 ± 0.234
3.53IleGly: 3.53 ± 0.53
0.998IleHis: 0.998 ± 0.272
3.607IleIle: 3.607 ± 0.712
2.84IleLys: 2.84 ± 0.579
3.147IleLeu: 3.147 ± 0.54
0.614IleMet: 0.614 ± 0.193
1.995IleAsn: 1.995 ± 0.361
3.07IlePro: 3.07 ± 0.609
2.84IleGln: 2.84 ± 0.834
2.993IleArg: 2.993 ± 0.531
2.302IleSer: 2.302 ± 0.564
3.761IleThr: 3.761 ± 0.765
4.144IleVal: 4.144 ± 0.552
0.537IleTrp: 0.537 ± 0.173
1.535IleTyr: 1.535 ± 0.344
0.0IleXaa: 0.0 ± 0.0
Lys
5.372LysAla: 5.372 ± 0.82
0.307LysCys: 0.307 ± 0.165
2.379LysAsp: 2.379 ± 0.504
3.454LysGlu: 3.454 ± 0.671
1.765LysPhe: 1.765 ± 0.358
3.761LysGly: 3.761 ± 0.429
0.921LysHis: 0.921 ± 0.268
1.612LysIle: 1.612 ± 0.325
2.226LysLys: 2.226 ± 0.488
4.605LysLeu: 4.605 ± 0.7
1.305LysMet: 1.305 ± 0.286
1.612LysAsn: 1.612 ± 0.362
3.914LysPro: 3.914 ± 0.63
1.305LysGln: 1.305 ± 0.352
3.07LysArg: 3.07 ± 0.541
1.842LysSer: 1.842 ± 0.373
2.686LysThr: 2.686 ± 0.361
5.219LysVal: 5.219 ± 0.586
1.074LysTrp: 1.074 ± 0.312
0.998LysTyr: 0.998 ± 0.278
0.0LysXaa: 0.0 ± 0.0
Leu
9.44LeuAla: 9.44 ± 0.841
0.537LeuCys: 0.537 ± 0.185
5.295LeuAsp: 5.295 ± 0.601
4.221LeuGlu: 4.221 ± 0.415
3.377LeuPhe: 3.377 ± 0.66
6.754LeuGly: 6.754 ± 0.852
2.072LeuHis: 2.072 ± 0.44
4.758LeuIle: 4.758 ± 0.85
3.684LeuLys: 3.684 ± 0.561
7.214LeuLeu: 7.214 ± 0.85
1.381LeuMet: 1.381 ± 0.313
3.607LeuAsn: 3.607 ± 0.486
4.682LeuPro: 4.682 ± 0.593
2.149LeuGln: 2.149 ± 0.401
5.065LeuArg: 5.065 ± 0.734
4.451LeuSer: 4.451 ± 0.598
4.912LeuThr: 4.912 ± 0.586
6.37LeuVal: 6.37 ± 1.056
1.228LeuTrp: 1.228 ± 0.257
1.535LeuTyr: 1.535 ± 0.324
0.0LeuXaa: 0.0 ± 0.0
Met
2.149MetAla: 2.149 ± 0.457
0.23MetCys: 0.23 ± 0.135
1.919MetAsp: 1.919 ± 0.347
1.381MetGlu: 1.381 ± 0.291
0.767MetPhe: 0.767 ± 0.292
1.612MetGly: 1.612 ± 0.527
0.384MetHis: 0.384 ± 0.168
0.998MetIle: 0.998 ± 0.313
0.998MetLys: 0.998 ± 0.278
1.381MetLeu: 1.381 ± 0.309
0.767MetMet: 0.767 ± 0.229
0.614MetAsn: 0.614 ± 0.232
1.612MetPro: 1.612 ± 0.353
1.151MetGln: 1.151 ± 0.28
1.151MetArg: 1.151 ± 0.283
2.609MetSer: 2.609 ± 0.48
1.688MetThr: 1.688 ± 0.35
1.151MetVal: 1.151 ± 0.32
0.307MetTrp: 0.307 ± 0.137
0.307MetTyr: 0.307 ± 0.133
0.0MetXaa: 0.0 ± 0.0
Asn
4.298AsnAla: 4.298 ± 0.557
0.077AsnCys: 0.077 ± 0.075
1.688AsnAsp: 1.688 ± 0.32
2.072AsnGlu: 2.072 ± 0.362
0.921AsnPhe: 0.921 ± 0.211
3.607AsnGly: 3.607 ± 0.489
0.614AsnHis: 0.614 ± 0.22
1.228AsnIle: 1.228 ± 0.232
1.612AsnLys: 1.612 ± 0.334
1.995AsnLeu: 1.995 ± 0.476
0.844AsnMet: 0.844 ± 0.211
0.691AsnAsn: 0.691 ± 0.216
2.379AsnPro: 2.379 ± 0.358
1.228AsnGln: 1.228 ± 0.278
1.765AsnArg: 1.765 ± 0.382
2.226AsnSer: 2.226 ± 0.452
2.379AsnThr: 2.379 ± 0.392
2.84AsnVal: 2.84 ± 0.45
0.537AsnTrp: 0.537 ± 0.218
1.074AsnTyr: 1.074 ± 0.285
0.0AsnXaa: 0.0 ± 0.0
Pro
5.602ProAla: 5.602 ± 0.687
0.46ProCys: 0.46 ± 0.18
3.07ProAsp: 3.07 ± 0.585
3.377ProGlu: 3.377 ± 0.574
1.151ProPhe: 1.151 ± 0.271
4.375ProGly: 4.375 ± 0.627
0.384ProHis: 0.384 ± 0.153
2.226ProIle: 2.226 ± 0.4
2.993ProLys: 2.993 ± 0.445
3.53ProLeu: 3.53 ± 0.503
0.844ProMet: 0.844 ± 0.241
1.458ProAsn: 1.458 ± 0.372
1.305ProPro: 1.305 ± 0.282
2.763ProGln: 2.763 ± 0.643
2.149ProArg: 2.149 ± 0.45
2.533ProSer: 2.533 ± 0.337
4.068ProThr: 4.068 ± 0.541
4.605ProVal: 4.605 ± 0.572
0.691ProTrp: 0.691 ± 0.248
1.612ProTyr: 1.612 ± 0.279
0.0ProXaa: 0.0 ± 0.0
Gln
7.291GlnAla: 7.291 ± 0.733
0.307GlnCys: 0.307 ± 0.177
2.149GlnAsp: 2.149 ± 0.342
3.3GlnGlu: 3.3 ± 0.512
0.614GlnPhe: 0.614 ± 0.188
3.377GlnGly: 3.377 ± 0.456
0.844GlnHis: 0.844 ± 0.247
1.842GlnIle: 1.842 ± 0.442
1.228GlnLys: 1.228 ± 0.337
3.991GlnLeu: 3.991 ± 0.675
0.844GlnMet: 0.844 ± 0.282
2.072GlnAsn: 2.072 ± 0.361
0.998GlnPro: 0.998 ± 0.227
2.226GlnGln: 2.226 ± 0.518
3.377GlnArg: 3.377 ± 0.506
2.072GlnSer: 2.072 ± 0.391
1.765GlnThr: 1.765 ± 0.267
2.686GlnVal: 2.686 ± 0.559
0.921GlnTrp: 0.921 ± 0.284
0.844GlnTyr: 0.844 ± 0.241
0.0GlnXaa: 0.0 ± 0.0
Arg
6.523ArgAla: 6.523 ± 0.722
0.537ArgCys: 0.537 ± 0.199
4.144ArgAsp: 4.144 ± 0.508
3.454ArgGlu: 3.454 ± 0.558
1.919ArgPhe: 1.919 ± 0.423
2.993ArgGly: 2.993 ± 0.645
1.151ArgHis: 1.151 ± 0.34
2.763ArgIle: 2.763 ± 0.472
3.53ArgLys: 3.53 ± 0.546
4.912ArgLeu: 4.912 ± 0.765
1.688ArgMet: 1.688 ± 0.282
1.995ArgAsn: 1.995 ± 0.487
3.147ArgPro: 3.147 ± 0.612
1.842ArgGln: 1.842 ± 0.352
4.835ArgArg: 4.835 ± 0.818
3.147ArgSer: 3.147 ± 0.428
2.84ArgThr: 2.84 ± 0.426
5.679ArgVal: 5.679 ± 0.709
1.151ArgTrp: 1.151 ± 0.387
1.305ArgTyr: 1.305 ± 0.362
0.0ArgXaa: 0.0 ± 0.0
Ser
5.756SerAla: 5.756 ± 1.072
0.384SerCys: 0.384 ± 0.188
3.07SerAsp: 3.07 ± 0.355
2.686SerGlu: 2.686 ± 0.493
1.688SerPhe: 1.688 ± 0.387
4.605SerGly: 4.605 ± 0.613
0.998SerHis: 0.998 ± 0.338
3.07SerIle: 3.07 ± 0.445
2.609SerLys: 2.609 ± 0.338
4.605SerLeu: 4.605 ± 0.708
2.149SerMet: 2.149 ± 0.49
2.149SerAsn: 2.149 ± 0.517
2.609SerPro: 2.609 ± 0.46
2.379SerGln: 2.379 ± 0.427
2.609SerArg: 2.609 ± 0.348
3.147SerSer: 3.147 ± 0.469
3.07SerThr: 3.07 ± 0.591
4.068SerVal: 4.068 ± 0.589
1.074SerTrp: 1.074 ± 0.274
2.149SerTyr: 2.149 ± 0.376
0.0SerXaa: 0.0 ± 0.0
Thr
6.754ThrAla: 6.754 ± 0.703
0.23ThrCys: 0.23 ± 0.115
3.3ThrAsp: 3.3 ± 0.547
2.686ThrGlu: 2.686 ± 0.39
2.993ThrPhe: 2.993 ± 0.493
4.912ThrGly: 4.912 ± 0.615
1.151ThrHis: 1.151 ± 0.306
3.684ThrIle: 3.684 ± 0.615
2.84ThrLys: 2.84 ± 0.504
5.295ThrLeu: 5.295 ± 0.663
1.151ThrMet: 1.151 ± 0.299
2.379ThrAsn: 2.379 ± 0.547
3.223ThrPro: 3.223 ± 0.451
2.763ThrGln: 2.763 ± 0.413
4.375ThrArg: 4.375 ± 0.534
4.144ThrSer: 4.144 ± 0.576
4.451ThrThr: 4.451 ± 0.592
5.909ThrVal: 5.909 ± 0.754
1.228ThrTrp: 1.228 ± 0.249
1.612ThrTyr: 1.612 ± 0.305
0.0ThrXaa: 0.0 ± 0.0
Val
8.058ValAla: 8.058 ± 0.787
0.384ValCys: 0.384 ± 0.174
4.758ValAsp: 4.758 ± 0.49
5.372ValGlu: 5.372 ± 0.709
1.995ValPhe: 1.995 ± 0.371
4.528ValGly: 4.528 ± 0.687
1.688ValHis: 1.688 ± 0.378
4.068ValIle: 4.068 ± 0.595
3.377ValLys: 3.377 ± 0.489
6.447ValLeu: 6.447 ± 0.682
1.305ValMet: 1.305 ± 0.296
2.456ValAsn: 2.456 ± 0.532
4.068ValPro: 4.068 ± 0.549
3.991ValGln: 3.991 ± 0.67
3.454ValArg: 3.454 ± 0.505
4.144ValSer: 4.144 ± 0.411
5.679ValThr: 5.679 ± 1.04
5.986ValVal: 5.986 ± 0.705
2.226ValTrp: 2.226 ± 0.448
1.765ValTyr: 1.765 ± 0.398
0.0ValXaa: 0.0 ± 0.0
Trp
1.074TrpAla: 1.074 ± 0.22
0.077TrpCys: 0.077 ± 0.072
1.535TrpAsp: 1.535 ± 0.386
1.074TrpGlu: 1.074 ± 0.346
0.767TrpPhe: 0.767 ± 0.233
0.844TrpGly: 0.844 ± 0.254
0.537TrpHis: 0.537 ± 0.187
0.998TrpIle: 0.998 ± 0.243
0.691TrpLys: 0.691 ± 0.208
1.919TrpLeu: 1.919 ± 0.426
0.384TrpMet: 0.384 ± 0.176
1.074TrpAsn: 1.074 ± 0.225
0.691TrpPro: 0.691 ± 0.24
0.537TrpGln: 0.537 ± 0.179
1.074TrpArg: 1.074 ± 0.262
1.228TrpSer: 1.228 ± 0.466
1.842TrpThr: 1.842 ± 0.41
1.151TrpVal: 1.151 ± 0.26
0.46TrpTrp: 0.46 ± 0.209
0.691TrpTyr: 0.691 ± 0.215
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.072TyrAla: 2.072 ± 0.335
0.307TyrCys: 0.307 ± 0.164
1.919TyrAsp: 1.919 ± 0.471
1.381TyrGlu: 1.381 ± 0.429
0.767TyrPhe: 0.767 ± 0.236
2.226TyrGly: 2.226 ± 0.353
0.998TyrHis: 0.998 ± 0.27
1.228TyrIle: 1.228 ± 0.282
1.151TyrLys: 1.151 ± 0.371
1.458TyrLeu: 1.458 ± 0.336
0.614TyrMet: 0.614 ± 0.2
0.921TyrAsn: 0.921 ± 0.22
1.765TyrPro: 1.765 ± 0.401
0.844TyrGln: 0.844 ± 0.281
2.149TyrArg: 2.149 ± 0.35
2.302TyrSer: 2.302 ± 0.451
1.305TyrThr: 1.305 ± 0.242
1.995TyrVal: 1.995 ± 0.395
0.384TyrTrp: 0.384 ± 0.187
0.767TyrTyr: 0.767 ± 0.278
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (13031 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski