Ruminiclostridium cellobioparum subsp. termitidis CT1112

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminiclostridium; Ruminiclostridium cellobioparum; Ruminiclostridium cellobioparum subsp. termitidis

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5302 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0FF57|S0FF57_RUMCE Stage 0 sporulation protein A homolog OS=Ruminiclostridium cellobioparum subsp. termitidis CT1112 OX=1195236 GN=CTER_5354 PE=4 SV=1
MM1 pKa = 7.46NLPTYY6 pKa = 10.78DD7 pKa = 3.88EE8 pKa = 5.38LLDD11 pKa = 3.97FYY13 pKa = 11.6YY14 pKa = 11.16NFLEE18 pKa = 4.39AEE20 pKa = 3.91QDD22 pKa = 3.56RR23 pKa = 11.84EE24 pKa = 4.06IEE26 pKa = 4.05EE27 pKa = 4.13NEE29 pKa = 4.13IIEE32 pKa = 4.21LLQVLLNGYY41 pKa = 8.08LIWLTILSSFGKK53 pKa = 9.59PLTIKK58 pKa = 10.35VLSGVTGDD66 pKa = 5.0DD67 pKa = 3.46EE68 pKa = 4.51NLPFIDD74 pKa = 5.49LYY76 pKa = 11.63SDD78 pKa = 4.12FQLDD82 pKa = 3.72FSDD85 pKa = 4.83FKK87 pKa = 11.32AYY89 pKa = 9.95QKK91 pKa = 10.77YY92 pKa = 10.36EE93 pKa = 4.14KK94 pKa = 10.65FLDD97 pKa = 4.12CQQ99 pKa = 3.43

Molecular weight: 11.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0FNR3|S0FNR3_RUMCE Phage tail protein OS=Ruminiclostridium cellobioparum subsp. termitidis CT1112 OX=1195236 GN=CTER_3468 PE=4 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.76QPKK8 pKa = 9.68RR9 pKa = 11.84RR10 pKa = 11.84HH11 pKa = 5.51AKK13 pKa = 9.71KK14 pKa = 9.42EE15 pKa = 3.5HH16 pKa = 5.93GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 7.55VLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.26GRR39 pKa = 11.84KK40 pKa = 8.78VLSAA44 pKa = 4.05

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5302

0

5302

1861788

22

3641

351.1

39.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.015 ± 0.041

1.196 ± 0.013

5.446 ± 0.026

6.598 ± 0.035

4.446 ± 0.029

7.074 ± 0.03

1.477 ± 0.014

8.35 ± 0.04

7.135 ± 0.032

9.081 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.018

5.408 ± 0.027

3.356 ± 0.02

2.973 ± 0.016

4.0 ± 0.025

6.585 ± 0.027

5.39 ± 0.039

6.652 ± 0.026

1.002 ± 0.013

4.11 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski