candidate division MSBL1 archaeon SCGC-AAA259I07

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 671 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133UMZ8|A0A133UMZ8_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA259I07 OX=1698266 GN=AKJ36_00395 PE=4 SV=1
MM1 pKa = 7.05ITLDD5 pKa = 4.05KK6 pKa = 10.34KK7 pKa = 10.64AQGSTEE13 pKa = 3.87YY14 pKa = 10.29MLILAVVLVVAGAIYY29 pKa = 9.84FITRR33 pKa = 11.84SGGNYY38 pKa = 8.29PAVSATPVAKK48 pKa = 10.3DD49 pKa = 3.56ANNDD53 pKa = 3.29GSDD56 pKa = 3.61DD57 pKa = 4.01LVILVEE63 pKa = 4.44ANSIPSGEE71 pKa = 4.02WKK73 pKa = 10.27VVYY76 pKa = 10.58NGTTVSPDD84 pKa = 3.21EE85 pKa = 4.67PLEE88 pKa = 3.98QPQYY92 pKa = 10.94IVDD95 pKa = 3.92DD96 pKa = 5.13NIPEE100 pKa = 4.02DD101 pKa = 3.93TYY103 pKa = 11.69EE104 pKa = 4.12VTLSHH109 pKa = 6.73IPSGHH114 pKa = 5.68VYY116 pKa = 9.3FNEE119 pKa = 5.08RR120 pKa = 11.84ITIQQ124 pKa = 3.06

Molecular weight: 13.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133ULR6|A0A133ULR6_9EURY Nucleic acid-binding protein OS=candidate division MSBL1 archaeon SCGC-AAA259I07 OX=1698266 GN=AKJ36_01360 PE=4 SV=1
MM1 pKa = 6.98GCARR5 pKa = 11.84VKK7 pKa = 10.44GAQQSLSARR16 pKa = 11.84LQRR19 pKa = 11.84EE20 pKa = 3.81CRR22 pKa = 11.84AIARR26 pKa = 11.84FLVGANEE33 pKa = 4.16MADD36 pKa = 3.21IFPPHH41 pKa = 6.58LSGVNEE47 pKa = 4.01MKK49 pKa = 10.78LGGGFAFRR57 pKa = 11.84PHH59 pKa = 6.97KK60 pKa = 10.5PPIRR64 pKa = 11.84YY65 pKa = 8.75ILCLIFQFSFQQLTSSLLFNRR86 pKa = 11.84ALVLVQLAKK95 pKa = 10.24EE96 pKa = 4.1LPSFVRR102 pKa = 11.84KK103 pKa = 10.05YY104 pKa = 10.45FGKK107 pKa = 10.34FDD109 pKa = 4.32LARR112 pKa = 11.84KK113 pKa = 9.56SSGSRR118 pKa = 11.84KK119 pKa = 9.43EE120 pKa = 3.81DD121 pKa = 3.12GRR123 pKa = 11.84DD124 pKa = 3.33YY125 pKa = 11.04RR126 pKa = 11.84QGARR130 pKa = 11.84SSS132 pKa = 3.29

Molecular weight: 14.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

671

0

671

145563

52

1198

216.9

24.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.681 ± 0.092

0.973 ± 0.037

5.697 ± 0.098

10.531 ± 0.162

4.14 ± 0.095

7.228 ± 0.104

1.622 ± 0.04

7.146 ± 0.088

7.768 ± 0.11

9.395 ± 0.126

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.23 ± 0.045

3.721 ± 0.073

3.849 ± 0.058

2.282 ± 0.042

5.879 ± 0.089

6.275 ± 0.072

4.692 ± 0.069

6.799 ± 0.089

1.079 ± 0.046

3.01 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski