Cowpea polerovirus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus; unclassified Polerovirus

Average proteome isoelectric point is 8.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U9RXY8|A0A1U9RXY8_9LUTE Serine protease OS=Cowpea polerovirus 2 OX=1913125 PE=4 SV=1
MM1 pKa = 7.39EE2 pKa = 5.57GEE4 pKa = 4.73LGVADD9 pKa = 3.88AFKK12 pKa = 10.93GVSAWLWSRR21 pKa = 11.84PLGYY25 pKa = 10.23HH26 pKa = 5.52VAEE29 pKa = 4.59EE30 pKa = 4.88DD31 pKa = 3.48NDD33 pKa = 3.66EE34 pKa = 4.24TAEE37 pKa = 4.09LLQEE41 pKa = 4.23EE42 pKa = 4.71ADD44 pKa = 4.0LEE46 pKa = 4.41EE47 pKa = 4.62GRR49 pKa = 11.84ATHH52 pKa = 7.03LCFHH56 pKa = 6.7RR57 pKa = 11.84TASKK61 pKa = 10.87AVTPDD66 pKa = 2.76ISRR69 pKa = 11.84SGRR72 pKa = 11.84LFQRR76 pKa = 11.84SQSSVMEE83 pKa = 4.04FSGPTMSIRR92 pKa = 11.84SQWSSWSSSPRR103 pKa = 11.84PLQPPRR109 pKa = 11.84VQSLSNWIPIASTVPYY125 pKa = 10.27NHH127 pKa = 7.5RR128 pKa = 11.84LTNSEE133 pKa = 3.96LSKK136 pKa = 11.7GEE138 pKa = 4.14IEE140 pKa = 4.94LGTPVKK146 pKa = 10.23STGLNGTMRR155 pKa = 11.84PKK157 pKa = 10.37INSGYY162 pKa = 7.71FTRR165 pKa = 11.84EE166 pKa = 3.26TEE168 pKa = 4.11APRR171 pKa = 11.84LRR173 pKa = 11.84GPSGLPSGANSRR185 pKa = 11.84TPNRR189 pKa = 3.78

Molecular weight: 20.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U9RYW5|A0A1U9RYW5_9LUTE Readthrough protein OS=Cowpea polerovirus 2 OX=1913125 PE=3 SV=1
MM1 pKa = 7.44NFGNFLINEE10 pKa = 4.18QGSCVTSLKK19 pKa = 10.21RR20 pKa = 11.84LSPRR24 pKa = 11.84AVEE27 pKa = 4.21YY28 pKa = 10.46FSVLAVVCQTFLTNDD43 pKa = 3.71YY44 pKa = 9.84PSSDD48 pKa = 3.25HH49 pKa = 6.96EE50 pKa = 4.69FILRR54 pKa = 11.84CFVFLLPFLLRR65 pKa = 11.84GPKK68 pKa = 10.15NIGVRR73 pKa = 11.84KK74 pKa = 9.59PGDD77 pKa = 3.23KK78 pKa = 10.35PGLVRR83 pKa = 11.84AKK85 pKa = 9.97QFYY88 pKa = 9.7AARR91 pKa = 11.84FAARR95 pKa = 11.84LGVYY99 pKa = 8.66TPSLSGVQRR108 pKa = 11.84TVSLRR113 pKa = 11.84LSNLEE118 pKa = 3.5FTRR121 pKa = 11.84NRR123 pKa = 11.84RR124 pKa = 11.84KK125 pKa = 7.1TTAILQRR132 pKa = 11.84HH133 pKa = 5.77HH134 pKa = 6.62ARR136 pKa = 11.84SVGARR141 pKa = 11.84IEE143 pKa = 3.97RR144 pKa = 11.84RR145 pKa = 11.84KK146 pKa = 10.65DD147 pKa = 3.2VLFGGEE153 pKa = 4.06RR154 pKa = 11.84YY155 pKa = 9.4FKK157 pKa = 10.8QFVQTYY163 pKa = 10.16CRR165 pKa = 11.84FLDD168 pKa = 3.57HH169 pKa = 7.44EE170 pKa = 4.58YY171 pKa = 11.29GRR173 pKa = 11.84DD174 pKa = 3.34LSKK177 pKa = 10.87PLLVSDD183 pKa = 4.55LRR185 pKa = 11.84VVLAAAHH192 pKa = 6.72DD193 pKa = 3.97YY194 pKa = 10.5SLRR197 pKa = 11.84VAFRR201 pKa = 11.84HH202 pKa = 5.41QEE204 pKa = 3.64FHH206 pKa = 7.28ARR208 pKa = 11.84ACRR211 pKa = 11.84CLALYY216 pKa = 9.86LISSLGEE223 pKa = 3.81DD224 pKa = 3.21SALVFWVNANLPRR237 pKa = 11.84SHH239 pKa = 7.64ILFHH243 pKa = 7.54SEE245 pKa = 3.68VVLAEE250 pKa = 4.09TLLGQEE256 pKa = 4.23LQKK259 pKa = 11.27LL260 pKa = 3.88

Molecular weight: 29.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3125

46

1080

446.4

49.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.752 ± 0.254

2.112 ± 0.287

4.096 ± 0.788

5.76 ± 0.579

4.928 ± 0.428

7.648 ± 0.511

1.76 ± 0.289

3.968 ± 0.507

5.568 ± 0.462

8.448 ± 1.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.376 ± 0.133

4.384 ± 0.367

5.76 ± 0.481

4.128 ± 0.392

7.04 ± 1.183

9.184 ± 0.672

5.184 ± 0.427

6.72 ± 0.565

2.112 ± 0.364

3.04 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski