Nitrosomonas communis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3064 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7KJC5|A0A0F7KJC5_9PROT 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB OS=Nitrosomonas communis OX=44574 GN=rlmB PE=3 SV=1
MM1 pKa = 7.22LQDD4 pKa = 4.14LNVSIIEE11 pKa = 4.31RR12 pKa = 11.84DD13 pKa = 3.34TSAGSKK19 pKa = 10.63DD20 pKa = 4.17DD21 pKa = 4.64IIFSEE26 pKa = 4.45PSNIVLNGYY35 pKa = 9.17IGNDD39 pKa = 3.5MLNGGAGNDD48 pKa = 3.73TLAGSSGNDD57 pKa = 2.88ILQGGSGRR65 pKa = 11.84DD66 pKa = 3.42LLMGGDD72 pKa = 4.05GDD74 pKa = 5.03DD75 pKa = 3.48ILRR78 pKa = 11.84GGMGEE83 pKa = 4.04NDD85 pKa = 4.21HH86 pKa = 7.18LRR88 pKa = 11.84GDD90 pKa = 4.13NGNDD94 pKa = 3.1TYY96 pKa = 11.7LFAAGDD102 pKa = 4.29GNTSIDD108 pKa = 3.76NNDD111 pKa = 3.21TGTSYY116 pKa = 11.57DD117 pKa = 3.29VLQFMEE123 pKa = 5.47GINPGEE129 pKa = 4.12VVVTRR134 pKa = 11.84DD135 pKa = 3.18SSNLYY140 pKa = 8.66LTLTSTSEE148 pKa = 4.2KK149 pKa = 9.2ITVSNYY155 pKa = 9.28FYY157 pKa = 10.77QDD159 pKa = 2.77GVGPYY164 pKa = 9.98VLDD167 pKa = 5.04AIEE170 pKa = 4.87FSDD173 pKa = 3.81GTSWDD178 pKa = 3.55VATVKK183 pKa = 10.51QKK185 pKa = 10.53VMQGTSEE192 pKa = 4.16ADD194 pKa = 3.23NLTGFASNDD203 pKa = 3.88TIDD206 pKa = 3.84GLEE209 pKa = 4.7GNDD212 pKa = 4.16SLNGAAGNDD221 pKa = 3.82TLLGGAGNDD230 pKa = 3.6TLSGSEE236 pKa = 4.3GNDD239 pKa = 2.91ILEE242 pKa = 4.97GGDD245 pKa = 3.78GTDD248 pKa = 3.06VLYY251 pKa = 11.0GGSGDD256 pKa = 3.84DD257 pKa = 3.97TLSGGAGANDD267 pKa = 4.54SLTGDD272 pKa = 4.24AGNDD276 pKa = 3.36TYY278 pKa = 11.68LFAAGEE284 pKa = 4.34GHH286 pKa = 6.34TSINNYY292 pKa = 7.73DD293 pKa = 3.75TSTSRR298 pKa = 11.84HH299 pKa = 5.37DD300 pKa = 3.36VLRR303 pKa = 11.84FLEE306 pKa = 5.26GINPGDD312 pKa = 3.58VLVSRR317 pKa = 11.84DD318 pKa = 3.45SSNLYY323 pKa = 8.64LTLAGTGEE331 pKa = 4.72KK332 pKa = 8.9ITVSSYY338 pKa = 10.47FYY340 pKa = 10.62QDD342 pKa = 2.75GAGPYY347 pKa = 9.88VLDD350 pKa = 5.45AIEE353 pKa = 4.44FSNGTSWDD361 pKa = 3.57VATVKK366 pKa = 10.64QKK368 pKa = 10.72VLQGTSGADD377 pKa = 3.16NLTGFASNDD386 pKa = 3.88TIDD389 pKa = 3.84GLEE392 pKa = 4.7GNDD395 pKa = 4.16SLNGAAGNDD404 pKa = 3.82TLLGGAGNDD413 pKa = 3.6TLSGSEE419 pKa = 4.34GNDD422 pKa = 3.2VLDD425 pKa = 5.02GGDD428 pKa = 4.09GTDD431 pKa = 3.4MLYY434 pKa = 11.04GGAGDD439 pKa = 4.13DD440 pKa = 4.17TLSGGAGTNDD450 pKa = 4.17SLTGDD455 pKa = 4.25AGNDD459 pKa = 3.36TYY461 pKa = 11.68LFAAGEE467 pKa = 4.34GHH469 pKa = 6.34TSINNYY475 pKa = 7.73DD476 pKa = 3.75TSTSRR481 pKa = 11.84HH482 pKa = 5.37DD483 pKa = 3.36VLRR486 pKa = 11.84FLEE489 pKa = 5.17GINPGEE495 pKa = 4.14VAVTRR500 pKa = 11.84NSSNLYY506 pKa = 8.77LTLTSTGEE514 pKa = 4.15KK515 pKa = 8.64ITVSNYY521 pKa = 8.98FYY523 pKa = 10.84QDD525 pKa = 2.81GVSPYY530 pKa = 10.46VLDD533 pKa = 5.43AIEE536 pKa = 4.87FSDD539 pKa = 3.81GTSWDD544 pKa = 3.55VATVKK549 pKa = 10.57QKK551 pKa = 9.17TLQGTSGADD560 pKa = 3.16NLTGFASNDD569 pKa = 3.42TMNGLAGNDD578 pKa = 3.73TLSGGNGNDD587 pKa = 3.35VLEE590 pKa = 5.25GGDD593 pKa = 4.18GTDD596 pKa = 3.12MLYY599 pKa = 10.85GGSGDD604 pKa = 3.8DD605 pKa = 3.97TLSGGAGANDD615 pKa = 4.54SLTGDD620 pKa = 4.24AGNDD624 pKa = 3.36TYY626 pKa = 11.68LFAAGEE632 pKa = 4.34GHH634 pKa = 6.34TSINNYY640 pKa = 6.6DD641 pKa = 3.79TSVGRR646 pKa = 11.84HH647 pKa = 5.02DD648 pKa = 4.07VLRR651 pKa = 11.84FMQGINPGEE660 pKa = 4.09VAVTRR665 pKa = 11.84NSSNLYY671 pKa = 8.8LTLQSTGEE679 pKa = 4.17KK680 pKa = 8.61ITINSYY686 pKa = 10.21FYY688 pKa = 10.72QDD690 pKa = 3.02GAGPYY695 pKa = 9.88VLDD698 pKa = 5.37AIEE701 pKa = 4.87FSDD704 pKa = 3.82GTSWDD709 pKa = 3.66VVTVKK714 pKa = 10.58QKK716 pKa = 9.28TLQGTSGADD725 pKa = 3.16NLTGFASNDD734 pKa = 3.42TMNGLAGNDD743 pKa = 3.73TLSGGNGNDD752 pKa = 3.35VLEE755 pKa = 5.25GGDD758 pKa = 4.18GTDD761 pKa = 3.12MLYY764 pKa = 10.85GGSGDD769 pKa = 3.8DD770 pKa = 3.97TLSGGAGTNDD780 pKa = 4.17SLTGDD785 pKa = 4.25AGNDD789 pKa = 3.36TYY791 pKa = 11.68LFAAGEE797 pKa = 4.34GHH799 pKa = 6.34TSINNYY805 pKa = 7.28DD806 pKa = 3.74TSISRR811 pKa = 11.84HH812 pKa = 5.21DD813 pKa = 3.42VLRR816 pKa = 11.84FLEE819 pKa = 5.17GINPGEE825 pKa = 4.14VAVTRR830 pKa = 11.84NSSNLYY836 pKa = 8.77LTLTSTGEE844 pKa = 4.15KK845 pKa = 8.64ITVSNYY851 pKa = 8.98FYY853 pKa = 10.84QDD855 pKa = 2.81GVSPYY860 pKa = 10.46VLDD863 pKa = 5.43AIEE866 pKa = 4.87FSDD869 pKa = 3.81GTSWDD874 pKa = 3.55VATVKK879 pKa = 10.57QKK881 pKa = 9.17TLQGTSGADD890 pKa = 3.16NLTGFASNDD899 pKa = 3.42TMNGLAGNDD908 pKa = 3.73TLSGGNGNDD917 pKa = 3.35VLEE920 pKa = 5.25GGDD923 pKa = 4.18GTDD926 pKa = 3.12MLYY929 pKa = 10.85GGSGDD934 pKa = 3.8DD935 pKa = 3.97TLSGGAGANDD945 pKa = 4.54SLTGDD950 pKa = 4.24AGNDD954 pKa = 3.36TYY956 pKa = 11.68LFAAGEE962 pKa = 4.34GHH964 pKa = 6.34TSINNYY970 pKa = 6.6DD971 pKa = 3.79TSVGRR976 pKa = 11.84HH977 pKa = 5.02DD978 pKa = 4.07VLRR981 pKa = 11.84FMQGINPGEE990 pKa = 4.09VAVTRR995 pKa = 11.84NSSNLYY1001 pKa = 8.8LTLQSTGEE1009 pKa = 4.17KK1010 pKa = 8.61ITINSYY1016 pKa = 10.21FYY1018 pKa = 10.72QDD1020 pKa = 3.02GAGPYY1025 pKa = 9.88VLDD1028 pKa = 5.37AIEE1031 pKa = 4.87FSDD1034 pKa = 3.82GTSWDD1039 pKa = 3.66VVTVKK1044 pKa = 10.58QKK1046 pKa = 9.28TLQGTSGADD1055 pKa = 3.16NLTGFASNDD1064 pKa = 3.42TMNGLAGNDD1073 pKa = 3.73TLSGGNGNDD1082 pKa = 3.35VLEE1085 pKa = 5.25GGDD1088 pKa = 4.18GTDD1091 pKa = 3.12MLYY1094 pKa = 10.85GGSGDD1099 pKa = 3.8DD1100 pKa = 3.97TLSGGAGANDD1110 pKa = 4.54SLTGDD1115 pKa = 4.24AGNDD1119 pKa = 3.36TYY1121 pKa = 11.68LFAAGEE1127 pKa = 4.64GNTSINNYY1135 pKa = 7.4DD1136 pKa = 3.65TGVGRR1141 pKa = 11.84HH1142 pKa = 5.76DD1143 pKa = 3.53VLRR1146 pKa = 11.84FLQGINPGDD1155 pKa = 3.61VLVSRR1160 pKa = 11.84DD1161 pKa = 3.45SSNLYY1166 pKa = 8.64LTLAGTGEE1174 pKa = 4.72KK1175 pKa = 8.9ITVSSYY1181 pKa = 10.47FYY1183 pKa = 10.62QDD1185 pKa = 2.75GAGPYY1190 pKa = 9.88VLDD1193 pKa = 5.37AIEE1196 pKa = 4.87FSDD1199 pKa = 3.81GTSWDD1204 pKa = 3.55VATVKK1209 pKa = 10.57QKK1211 pKa = 9.17TLQGTSGADD1220 pKa = 3.16NLTGFASNDD1229 pKa = 3.38SMNGLAGNDD1238 pKa = 3.73TLSGGNGNDD1247 pKa = 3.5VLDD1250 pKa = 4.97GGDD1253 pKa = 4.09GTDD1256 pKa = 3.4MLYY1259 pKa = 11.04GGAGDD1264 pKa = 4.13DD1265 pKa = 4.17TLSGGAGTNDD1275 pKa = 4.17SLTGDD1280 pKa = 4.25AGNDD1284 pKa = 3.36TYY1286 pKa = 11.68LFAAGEE1292 pKa = 4.64GNTSINNYY1300 pKa = 6.4DD1301 pKa = 3.7TSVGRR1306 pKa = 11.84HH1307 pKa = 5.14DD1308 pKa = 3.45VLRR1311 pKa = 11.84FLEE1314 pKa = 5.26GINPGDD1320 pKa = 3.58VLVSRR1325 pKa = 11.84DD1326 pKa = 3.45SSNLYY1331 pKa = 8.64LTLAGTGEE1339 pKa = 4.72KK1340 pKa = 8.9ITVSSYY1346 pKa = 10.47FYY1348 pKa = 10.62QDD1350 pKa = 2.75GAGPYY1355 pKa = 9.88VLDD1358 pKa = 5.37AIEE1361 pKa = 4.87FSDD1364 pKa = 3.81GTSWDD1369 pKa = 3.55VATVKK1374 pKa = 10.57QKK1376 pKa = 9.17TLQGTSGADD1385 pKa = 3.16NLTGFASNDD1394 pKa = 3.88TIDD1397 pKa = 3.84GLEE1400 pKa = 4.7GNDD1403 pKa = 4.16SLNGAAGNDD1412 pKa = 3.82TLLGGAGNDD1421 pKa = 3.33ILSGSDD1427 pKa = 3.35GNDD1430 pKa = 3.09VLEE1433 pKa = 4.99GSDD1436 pKa = 4.48GSDD1439 pKa = 2.92MLYY1442 pKa = 10.98GGAGDD1447 pKa = 3.62DD1448 pKa = 3.86TLRR1451 pKa = 11.84GGSGGNDD1458 pKa = 3.52SLTGDD1463 pKa = 4.39AGNDD1467 pKa = 3.15TYY1469 pKa = 11.53LFKK1472 pKa = 10.91ISDD1475 pKa = 3.84AKK1477 pKa = 11.06DD1478 pKa = 2.99IINNYY1483 pKa = 8.4DD1484 pKa = 3.39TDD1486 pKa = 3.86VKK1488 pKa = 11.2SFDD1491 pKa = 3.43VLRR1494 pKa = 11.84ITEE1497 pKa = 4.08VSFEE1501 pKa = 4.15NLWFSRR1507 pKa = 11.84NGSNLQINIAGTDD1520 pKa = 3.71DD1521 pKa = 3.89QLTITNWYY1529 pKa = 9.45SGNTYY1534 pKa = 10.5QLDD1537 pKa = 3.79QITADD1542 pKa = 3.3SSSLLKK1548 pKa = 10.6EE1549 pKa = 4.41HH1550 pKa = 6.94VDD1552 pKa = 3.62QLVSAMSSYY1561 pKa = 10.63KK1562 pKa = 10.39VPSGEE1567 pKa = 4.42GNIISQDD1574 pKa = 3.26VMNALQPVFNEE1585 pKa = 3.72VWLL1588 pKa = 4.14

Molecular weight: 163.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7KBS1|A0A0F7KBS1_9PROT Outer membrane protein assembly factor BamA OS=Nitrosomonas communis OX=44574 GN=bamA PE=3 SV=1
MM1 pKa = 7.87DD2 pKa = 5.58ASLISILLGSFTGIVLALTGAGGTIIAVPLLMFSLDD38 pKa = 3.53LTVAQAAPISLLAVSLSGAIGAWLAFRR65 pKa = 11.84QKK67 pKa = 10.09RR68 pKa = 11.84VRR70 pKa = 11.84YY71 pKa = 8.73RR72 pKa = 11.84AASFIALIGALISPAGIWLAQHH94 pKa = 6.71LPDD97 pKa = 5.28ISLTLLFAGVLLFVAQHH114 pKa = 5.99MFRR117 pKa = 11.84QSMHH121 pKa = 5.93EE122 pKa = 4.35GKK124 pKa = 10.08NEE126 pKa = 3.49MSTQHH131 pKa = 6.24SASPCQIDD139 pKa = 3.9EE140 pKa = 4.28ASGRR144 pKa = 11.84LIWTIPCFRR153 pKa = 11.84ALALSGTTAGFLSGLLGVGGGFVIVPALRR182 pKa = 11.84KK183 pKa = 8.46ITNLPILSIMATSLAITALIATTGVISAIIIRR215 pKa = 11.84AMQWPIALFFCTGTIAGMLLGRR237 pKa = 11.84MFAEE241 pKa = 4.32RR242 pKa = 11.84LAGPRR247 pKa = 11.84LQQGFAILAGGIAAGMITKK266 pKa = 8.61VIWAILLL273 pKa = 3.89

Molecular weight: 28.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3064

0

3064

915887

42

5495

298.9

33.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.953 ± 0.043

1.047 ± 0.017

5.059 ± 0.032

6.147 ± 0.04

4.024 ± 0.029

6.812 ± 0.049

2.608 ± 0.02

6.893 ± 0.04

4.866 ± 0.05

10.658 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.022

3.954 ± 0.035

4.447 ± 0.03

4.32 ± 0.034

5.668 ± 0.039

5.973 ± 0.036

5.267 ± 0.036

6.411 ± 0.042

1.383 ± 0.018

3.003 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski