Microcaecilia unicolor

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Gymnophiona; Siphonopidae; Microcaecilia

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30305 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P7X3Q8|A0A6P7X3Q8_9AMPH E3 ubiquitin-protein ligase TRIM39-like OS=Microcaecilia unicolor OX=1415580 GN=LOC115459515 PE=4 SV=1
LL1 pKa = 7.54RR2 pKa = 11.84KK3 pKa = 8.26TSKK6 pKa = 11.0GDD8 pKa = 4.19DD9 pKa = 3.26ILEE12 pKa = 4.44QGDD15 pKa = 3.51VWQSAEE21 pKa = 4.19KK22 pKa = 9.58WCSQLFCAGVPMMEE36 pKa = 4.37EE37 pKa = 3.95NEE39 pKa = 4.28EE40 pKa = 4.05EE41 pKa = 5.16DD42 pKa = 4.38RR43 pKa = 11.84MQLFDD48 pKa = 5.32LDD50 pKa = 4.8CFLSDD55 pKa = 4.37ISDD58 pKa = 3.82TLFTMTQGMCAPPTVSDD75 pKa = 3.99CDD77 pKa = 3.75YY78 pKa = 11.32AGNADD83 pKa = 4.71MIQPDD88 pKa = 4.43LTQLQPNLDD97 pKa = 4.18DD98 pKa = 5.87FMDD101 pKa = 3.97IPEE104 pKa = 4.62LSLTKK109 pKa = 10.27HH110 pKa = 6.17CLWSDD115 pKa = 3.22QQNYY119 pKa = 9.82SKK121 pKa = 10.97VIRR124 pKa = 11.84FLLDD128 pKa = 2.89ILIIQILNIGVGFSSLLQSYY148 pKa = 9.92

Molecular weight: 16.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P7YPY7|A0A6P7YPY7_9AMPH Histone acetyltransferase OS=Microcaecilia unicolor OX=1415580 GN=KAT8 PE=3 SV=1
MM1 pKa = 7.57SSHH4 pKa = 6.01KK5 pKa = 9.07TFKK8 pKa = 10.28IKK10 pKa = 10.6RR11 pKa = 11.84FLAKK15 pKa = 9.68KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20580

9725

30305

18831892

31

38163

621.4

69.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.998 ± 0.015

2.277 ± 0.013

4.947 ± 0.01

7.273 ± 0.02

3.758 ± 0.011

5.787 ± 0.019

2.622 ± 0.008

5.004 ± 0.011

6.455 ± 0.016

9.457 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.346 ± 0.006

4.164 ± 0.01

5.387 ± 0.016

4.966 ± 0.021

5.197 ± 0.013

8.743 ± 0.019

5.737 ± 0.03

5.953 ± 0.012

1.116 ± 0.004

2.8 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski