Coprobacillus cateniformis
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3839 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7G850|E7G850_9FIRM ABC superfamily ATP binding cassette transporter OS=Coprobacillus cateniformis OX=100884 GN=HMPREF9488_00938 PE=4 SV=1
MM1 pKa = 7.26 GKK3 pKa = 10.54 LYY5 pKa = 10.23 ISYY8 pKa = 10.14 EE9 pKa = 4.33 AIEE12 pKa = 4.54 EE13 pKa = 4.03 MGKK16 pKa = 10.39 DD17 pKa = 3.38 YY18 pKa = 11.25 FEE20 pKa = 4.47 NKK22 pKa = 9.99 SYY24 pKa = 11.27 EE25 pKa = 4.22 DD26 pKa = 3.88 YY27 pKa = 11.7 VDD29 pKa = 3.64 LAKK32 pKa = 10.5 FDD34 pKa = 4.08 IVHH37 pKa = 6.98 LDD39 pKa = 3.45 DD40 pKa = 6.09 GLWLQQDD47 pKa = 4.17 DD48 pKa = 5.53 DD49 pKa = 5.17 GDD51 pKa = 4.42 LYY53 pKa = 10.74 ISYY56 pKa = 10.84 NDD58 pKa = 3.37 YY59 pKa = 11.65 DD60 pKa = 4.56 DD61 pKa = 4.34 FQFYY65 pKa = 10.73 DD66 pKa = 3.72 VEE68 pKa = 4.26 EE69 pKa = 4.32 LSNLKK74 pKa = 10.35 NADD77 pKa = 3.52 TVLKK81 pKa = 10.57 DD82 pKa = 4.47 FKK84 pKa = 11.36
Molecular weight: 10.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.528
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.719
Rodwell 3.579
Grimsley 3.439
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.139
Thurlkill 3.592
EMBOSS 3.719
Sillero 3.872
Patrickios 0.947
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|E7GB63|E7GB63_9FIRM Uncharacterized protein OS=Coprobacillus cateniformis OX=100884 GN=HMPREF9488_02004 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.51 RR10 pKa = 11.84 KK11 pKa = 9.77 RR12 pKa = 11.84 SKK14 pKa = 8.62 THH16 pKa = 5.5 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.08 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.02 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.93 VLSAA44 pKa = 4.11
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3839
0
3839
1123468
32
6836
292.6
33.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.48 ± 0.042
1.411 ± 0.019
5.864 ± 0.039
6.801 ± 0.043
4.543 ± 0.036
5.897 ± 0.047
2.283 ± 0.022
9.118 ± 0.046
7.635 ± 0.04
9.296 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.072 ± 0.027
5.282 ± 0.039
2.845 ± 0.027
4.038 ± 0.033
3.284 ± 0.025
6.022 ± 0.031
5.218 ± 0.039
6.247 ± 0.038
0.74 ± 0.014
4.925 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here