Cyanophage S-RIM32

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 230 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A127KMS6|A0A127KMS6_9CAUD High-light inducible protein OS=Cyanophage S-RIM32 OX=1278479 GN=R1080702_211 PE=4 SV=1
MM1 pKa = 8.04DD2 pKa = 5.11ALKK5 pKa = 10.94SEE7 pKa = 4.7VYY9 pKa = 10.76SDD11 pKa = 3.36TEE13 pKa = 3.87AYY15 pKa = 9.55KK16 pKa = 10.44IQLQQGGQKK25 pKa = 10.48FGFDD29 pKa = 4.01YY30 pKa = 11.09SDD32 pKa = 4.32SDD34 pKa = 4.37DD35 pKa = 5.3DD36 pKa = 5.1GYY38 pKa = 10.48PDD40 pKa = 3.39

Molecular weight: 4.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A127KM82|A0A127KM82_9CAUD CobS OS=Cyanophage S-RIM32 OX=1278479 GN=R1080702_148 PE=4 SV=1
MM1 pKa = 7.91AKK3 pKa = 10.05RR4 pKa = 11.84PSLTNGKK11 pKa = 8.15TIEE14 pKa = 4.39SKK16 pKa = 10.64PKK18 pKa = 9.15STRR21 pKa = 11.84QGCSKK26 pKa = 8.29NTKK29 pKa = 9.18YY30 pKa = 10.85AATSRR35 pKa = 11.84NNARR39 pKa = 11.84KK40 pKa = 9.07PYY42 pKa = 9.67RR43 pKa = 11.84GQGKK47 pKa = 9.57

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

230

0

230

59962

37

4021

260.7

28.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.674 ± 0.236

0.806 ± 0.08

6.716 ± 0.125

6.287 ± 0.284

4.409 ± 0.105

7.94 ± 0.343

1.339 ± 0.125

6.481 ± 0.138

5.587 ± 0.392

7.081 ± 0.128

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.88 ± 0.187

6.116 ± 0.236

4.044 ± 0.135

3.687 ± 0.102

3.948 ± 0.115

7.166 ± 0.249

7.623 ± 0.362

6.878 ± 0.163

1.057 ± 0.087

4.281 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski