Amino acid dipepetide frequency for Cyanophage S-RIM32

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.537AlaAla: 5.537 ± 0.504
0.75AlaCys: 0.75 ± 0.12
3.953AlaAsp: 3.953 ± 0.29
3.669AlaGlu: 3.669 ± 0.294
2.668AlaPhe: 2.668 ± 0.254
6.454AlaGly: 6.454 ± 0.405
0.834AlaHis: 0.834 ± 0.112
3.953AlaIle: 3.953 ± 0.236
3.702AlaLys: 3.702 ± 0.307
4.753AlaLeu: 4.753 ± 0.368
1.384AlaMet: 1.384 ± 0.181
3.736AlaAsn: 3.736 ± 0.384
2.902AlaPro: 2.902 ± 0.305
2.518AlaGln: 2.518 ± 0.221
2.285AlaArg: 2.285 ± 0.213
5.404AlaSer: 5.404 ± 0.334
5.287AlaThr: 5.287 ± 0.413
4.019AlaVal: 4.019 ± 0.275
0.634AlaTrp: 0.634 ± 0.129
2.301AlaTyr: 2.301 ± 0.205
0.0AlaXaa: 0.0 ± 0.0
Cys
0.717CysAla: 0.717 ± 0.122
0.133CysCys: 0.133 ± 0.055
0.634CysAsp: 0.634 ± 0.106
0.684CysGlu: 0.684 ± 0.109
0.367CysPhe: 0.367 ± 0.089
0.65CysGly: 0.65 ± 0.13
0.15CysHis: 0.15 ± 0.052
0.484CysIle: 0.484 ± 0.085
0.5CysLys: 0.5 ± 0.105
0.45CysLeu: 0.45 ± 0.094
0.233CysMet: 0.233 ± 0.069
0.517CysAsn: 0.517 ± 0.095
0.367CysPro: 0.367 ± 0.083
0.317CysGln: 0.317 ± 0.072
0.25CysArg: 0.25 ± 0.066
0.484CysSer: 0.484 ± 0.083
0.334CysThr: 0.334 ± 0.077
0.5CysVal: 0.5 ± 0.097
0.033CysTrp: 0.033 ± 0.026
0.25CysTyr: 0.25 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
4.903AspAla: 4.903 ± 0.331
0.5AspCys: 0.5 ± 0.099
4.87AspAsp: 4.87 ± 0.388
4.269AspGlu: 4.269 ± 0.369
3.636AspPhe: 3.636 ± 0.234
5.904AspGly: 5.904 ± 0.393
0.934AspHis: 0.934 ± 0.16
4.753AspIle: 4.753 ± 0.326
3.352AspLys: 3.352 ± 0.283
4.803AspLeu: 4.803 ± 0.27
1.351AspMet: 1.351 ± 0.199
3.719AspAsn: 3.719 ± 0.268
3.419AspPro: 3.419 ± 0.296
2.001AspGln: 2.001 ± 0.2
2.518AspArg: 2.518 ± 0.234
3.853AspSer: 3.853 ± 0.323
4.219AspThr: 4.219 ± 0.296
4.52AspVal: 4.52 ± 0.264
1.017AspTrp: 1.017 ± 0.179
2.618AspTyr: 2.618 ± 0.221
0.0AspXaa: 0.0 ± 0.0
Glu
3.536GluAla: 3.536 ± 0.277
0.484GluCys: 0.484 ± 0.093
4.253GluAsp: 4.253 ± 0.314
5.07GluGlu: 5.07 ± 0.624
2.952GluPhe: 2.952 ± 0.231
4.103GluGly: 4.103 ± 0.251
1.034GluHis: 1.034 ± 0.158
4.77GluIle: 4.77 ± 0.332
3.619GluLys: 3.619 ± 0.5
4.887GluLeu: 4.887 ± 0.293
1.634GluMet: 1.634 ± 0.254
3.336GluAsn: 3.336 ± 0.278
1.668GluPro: 1.668 ± 0.183
2.535GluGln: 2.535 ± 0.248
2.568GluArg: 2.568 ± 0.248
4.203GluSer: 4.203 ± 0.262
3.569GluThr: 3.569 ± 0.304
4.77GluVal: 4.77 ± 0.322
0.934GluTrp: 0.934 ± 0.133
2.952GluTyr: 2.952 ± 0.225
0.0GluXaa: 0.0 ± 0.0
Phe
2.235PheAla: 2.235 ± 0.181
0.484PheCys: 0.484 ± 0.104
3.536PheAsp: 3.536 ± 0.268
3.119PheGlu: 3.119 ± 0.276
1.668PhePhe: 1.668 ± 0.18
3.169PheGly: 3.169 ± 0.228
0.634PheHis: 0.634 ± 0.111
2.869PheIle: 2.869 ± 0.183
2.668PheLys: 2.668 ± 0.232
3.019PheLeu: 3.019 ± 0.218
0.734PheMet: 0.734 ± 0.148
2.535PheAsn: 2.535 ± 0.258
1.718PhePro: 1.718 ± 0.18
1.451PheGln: 1.451 ± 0.17
1.668PheArg: 1.668 ± 0.172
3.219PheSer: 3.219 ± 0.248
3.519PheThr: 3.519 ± 0.288
3.386PheVal: 3.386 ± 0.247
0.35PheTrp: 0.35 ± 0.092
2.118PheTyr: 2.118 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
5.554GlyAla: 5.554 ± 0.441
0.484GlyCys: 0.484 ± 0.095
5.47GlyAsp: 5.47 ± 0.468
4.686GlyGlu: 4.686 ± 0.24
2.902GlyPhe: 2.902 ± 0.239
8.856GlyGly: 8.856 ± 0.998
0.884GlyHis: 0.884 ± 0.15
4.486GlyIle: 4.486 ± 0.253
3.686GlyLys: 3.686 ± 0.322
4.586GlyLeu: 4.586 ± 0.335
1.451GlyMet: 1.451 ± 0.218
4.903GlyAsn: 4.903 ± 0.486
2.101GlyPro: 2.101 ± 0.25
2.602GlyGln: 2.602 ± 0.211
2.902GlyArg: 2.902 ± 0.256
7.421GlySer: 7.421 ± 0.654
7.238GlyThr: 7.238 ± 0.7
5.287GlyVal: 5.287 ± 0.336
0.801GlyTrp: 0.801 ± 0.157
3.102GlyTyr: 3.102 ± 0.282
0.0GlyXaa: 0.0 ± 0.0
His
0.584HisAla: 0.584 ± 0.131
0.133HisCys: 0.133 ± 0.046
0.867HisAsp: 0.867 ± 0.174
0.884HisGlu: 0.884 ± 0.136
0.667HisPhe: 0.667 ± 0.107
0.884HisGly: 0.884 ± 0.116
0.367HisHis: 0.367 ± 0.084
0.834HisIle: 0.834 ± 0.123
0.884HisLys: 0.884 ± 0.127
0.917HisLeu: 0.917 ± 0.162
0.334HisMet: 0.334 ± 0.094
0.684HisAsn: 0.684 ± 0.121
0.684HisPro: 0.684 ± 0.092
0.45HisGln: 0.45 ± 0.081
0.617HisArg: 0.617 ± 0.121
0.717HisSer: 0.717 ± 0.108
0.801HisThr: 0.801 ± 0.125
0.901HisVal: 0.901 ± 0.155
0.267HisTrp: 0.267 ± 0.067
0.917HisTyr: 0.917 ± 0.134
0.0HisXaa: 0.0 ± 0.0
Ile
4.386IleAla: 4.386 ± 0.284
0.567IleCys: 0.567 ± 0.121
5.654IleAsp: 5.654 ± 0.328
3.919IleGlu: 3.919 ± 0.231
2.318IlePhe: 2.318 ± 0.159
4.269IleGly: 4.269 ± 0.254
0.6IleHis: 0.6 ± 0.109
3.886IleIle: 3.886 ± 0.27
4.003IleLys: 4.003 ± 0.359
4.286IleLeu: 4.286 ± 0.343
0.934IleMet: 0.934 ± 0.13
4.52IleAsn: 4.52 ± 0.31
2.902IlePro: 2.902 ± 0.252
2.585IleGln: 2.585 ± 0.235
2.752IleArg: 2.752 ± 0.233
4.937IleSer: 4.937 ± 0.31
5.637IleThr: 5.637 ± 0.395
3.769IleVal: 3.769 ± 0.305
0.567IleTrp: 0.567 ± 0.09
2.318IleTyr: 2.318 ± 0.217
0.0IleXaa: 0.0 ± 0.0
Lys
3.202LysAla: 3.202 ± 0.291
0.534LysCys: 0.534 ± 0.091
3.135LysAsp: 3.135 ± 0.326
3.686LysGlu: 3.686 ± 0.451
2.668LysPhe: 2.668 ± 0.252
3.302LysGly: 3.302 ± 0.282
1.101LysHis: 1.101 ± 0.16
3.819LysIle: 3.819 ± 0.321
4.436LysLys: 4.436 ± 0.578
4.053LysLeu: 4.053 ± 0.323
1.651LysMet: 1.651 ± 0.231
3.019LysAsn: 3.019 ± 0.27
1.851LysPro: 1.851 ± 0.19
2.502LysGln: 2.502 ± 0.278
2.685LysArg: 2.685 ± 0.217
3.702LysSer: 3.702 ± 0.268
3.135LysThr: 3.135 ± 0.264
4.053LysVal: 4.053 ± 0.284
0.567LysTrp: 0.567 ± 0.112
2.768LysTyr: 2.768 ± 0.309
0.0LysXaa: 0.0 ± 0.0
Leu
4.77LeuAla: 4.77 ± 0.316
0.517LeuCys: 0.517 ± 0.118
5.12LeuAsp: 5.12 ± 0.3
4.67LeuGlu: 4.67 ± 0.294
2.802LeuPhe: 2.802 ± 0.224
4.686LeuGly: 4.686 ± 0.355
1.051LeuHis: 1.051 ± 0.126
4.536LeuIle: 4.536 ± 0.268
4.269LeuLys: 4.269 ± 0.394
4.37LeuLeu: 4.37 ± 0.348
1.601LeuMet: 1.601 ± 0.217
4.953LeuAsn: 4.953 ± 0.344
3.302LeuPro: 3.302 ± 0.221
2.735LeuGln: 2.735 ± 0.229
3.219LeuArg: 3.219 ± 0.222
5.003LeuSer: 5.003 ± 0.253
5.537LeuThr: 5.537 ± 0.383
4.403LeuVal: 4.403 ± 0.298
0.5LeuTrp: 0.5 ± 0.091
2.768LeuTyr: 2.768 ± 0.228
0.0LeuXaa: 0.0 ± 0.0
Met
1.418MetAla: 1.418 ± 0.26
0.233MetCys: 0.233 ± 0.08
1.151MetAsp: 1.151 ± 0.204
0.984MetGlu: 0.984 ± 0.162
1.001MetPhe: 1.001 ± 0.159
1.001MetGly: 1.001 ± 0.126
0.3MetHis: 0.3 ± 0.088
1.101MetIle: 1.101 ± 0.156
1.284MetLys: 1.284 ± 0.213
1.668MetLeu: 1.668 ± 0.205
0.5MetMet: 0.5 ± 0.119
1.134MetAsn: 1.134 ± 0.182
0.934MetPro: 0.934 ± 0.118
0.65MetGln: 0.65 ± 0.121
0.634MetArg: 0.634 ± 0.107
1.351MetSer: 1.351 ± 0.183
1.451MetThr: 1.451 ± 0.2
1.034MetVal: 1.034 ± 0.161
0.3MetTrp: 0.3 ± 0.079
0.667MetTyr: 0.667 ± 0.133
0.0MetXaa: 0.0 ± 0.0
Asn
4.069AsnAla: 4.069 ± 0.318
0.367AsnCys: 0.367 ± 0.074
3.185AsnAsp: 3.185 ± 0.298
3.369AsnGlu: 3.369 ± 0.239
3.202AsnPhe: 3.202 ± 0.224
5.037AsnGly: 5.037 ± 0.354
0.634AsnHis: 0.634 ± 0.096
4.57AsnIle: 4.57 ± 0.267
3.152AsnLys: 3.152 ± 0.259
4.887AsnLeu: 4.887 ± 0.318
0.901AsnMet: 0.901 ± 0.128
4.069AsnAsn: 4.069 ± 0.303
3.052AsnPro: 3.052 ± 0.255
2.101AsnGln: 2.101 ± 0.166
2.335AsnArg: 2.335 ± 0.155
4.119AsnSer: 4.119 ± 0.264
4.62AsnThr: 4.62 ± 0.408
4.169AsnVal: 4.169 ± 0.283
0.55AsnTrp: 0.55 ± 0.098
2.768AsnTyr: 2.768 ± 0.253
0.0AsnXaa: 0.0 ± 0.0
Pro
3.085ProAla: 3.085 ± 0.285
0.334ProCys: 0.334 ± 0.082
2.952ProAsp: 2.952 ± 0.309
3.002ProGlu: 3.002 ± 0.241
1.501ProPhe: 1.501 ± 0.166
2.952ProGly: 2.952 ± 0.28
0.417ProHis: 0.417 ± 0.094
2.168ProIle: 2.168 ± 0.182
1.968ProLys: 1.968 ± 0.201
2.485ProLeu: 2.485 ± 0.221
0.517ProMet: 0.517 ± 0.121
2.685ProAsn: 2.685 ± 0.215
1.784ProPro: 1.784 ± 0.194
1.568ProGln: 1.568 ± 0.197
1.351ProArg: 1.351 ± 0.154
2.885ProSer: 2.885 ± 0.254
3.569ProThr: 3.569 ± 0.296
2.985ProVal: 2.985 ± 0.274
0.534ProTrp: 0.534 ± 0.094
1.701ProTyr: 1.701 ± 0.177
0.0ProXaa: 0.0 ± 0.0
Gln
2.101GlnAla: 2.101 ± 0.19
0.217GlnCys: 0.217 ± 0.054
1.751GlnAsp: 1.751 ± 0.159
2.301GlnGlu: 2.301 ± 0.258
1.801GlnPhe: 1.801 ± 0.182
2.385GlnGly: 2.385 ± 0.219
0.484GlnHis: 0.484 ± 0.1
2.919GlnIle: 2.919 ± 0.235
2.352GlnLys: 2.352 ± 0.255
3.102GlnLeu: 3.102 ± 0.229
0.867GlnMet: 0.867 ± 0.156
2.101GlnAsn: 2.101 ± 0.174
1.634GlnPro: 1.634 ± 0.172
1.568GlnGln: 1.568 ± 0.236
1.668GlnArg: 1.668 ± 0.17
2.285GlnSer: 2.285 ± 0.181
2.335GlnThr: 2.335 ± 0.181
2.385GlnVal: 2.385 ± 0.18
0.55GlnTrp: 0.55 ± 0.102
2.068GlnTyr: 2.068 ± 0.193
0.0GlnXaa: 0.0 ± 0.0
Arg
2.852ArgAla: 2.852 ± 0.225
0.367ArgCys: 0.367 ± 0.079
2.335ArgAsp: 2.335 ± 0.2
2.485ArgGlu: 2.485 ± 0.213
2.068ArgPhe: 2.068 ± 0.168
2.835ArgGly: 2.835 ± 0.24
0.567ArgHis: 0.567 ± 0.116
2.635ArgIle: 2.635 ± 0.242
2.201ArgLys: 2.201 ± 0.241
3.119ArgLeu: 3.119 ± 0.219
0.867ArgMet: 0.867 ± 0.128
2.051ArgAsn: 2.051 ± 0.197
1.351ArgPro: 1.351 ± 0.137
1.568ArgGln: 1.568 ± 0.203
2.168ArgArg: 2.168 ± 0.284
2.368ArgSer: 2.368 ± 0.234
2.301ArgThr: 2.301 ± 0.214
2.618ArgVal: 2.618 ± 0.22
0.45ArgTrp: 0.45 ± 0.089
2.268ArgTyr: 2.268 ± 0.2
0.0ArgXaa: 0.0 ± 0.0
Ser
4.72SerAla: 4.72 ± 0.354
0.534SerCys: 0.534 ± 0.11
4.47SerAsp: 4.47 ± 0.261
3.636SerGlu: 3.636 ± 0.287
3.285SerPhe: 3.285 ± 0.239
7.488SerGly: 7.488 ± 0.573
0.801SerHis: 0.801 ± 0.114
4.786SerIle: 4.786 ± 0.447
3.252SerLys: 3.252 ± 0.262
4.887SerLeu: 4.887 ± 0.307
1.151SerMet: 1.151 ± 0.154
4.703SerAsn: 4.703 ± 0.385
2.785SerPro: 2.785 ± 0.241
2.068SerGln: 2.068 ± 0.179
2.218SerArg: 2.218 ± 0.206
5.921SerSer: 5.921 ± 0.54
5.921SerThr: 5.921 ± 0.385
5.27SerVal: 5.27 ± 0.29
0.7SerTrp: 0.7 ± 0.118
3.069SerTyr: 3.069 ± 0.267
0.0SerXaa: 0.0 ± 0.0
Thr
5.437ThrAla: 5.437 ± 0.421
0.45ThrCys: 0.45 ± 0.09
4.753ThrAsp: 4.753 ± 0.288
4.386ThrGlu: 4.386 ± 0.361
3.252ThrPhe: 3.252 ± 0.272
7.255ThrGly: 7.255 ± 0.837
0.984ThrHis: 0.984 ± 0.119
5.053ThrIle: 5.053 ± 0.452
3.786ThrLys: 3.786 ± 0.311
5.72ThrLeu: 5.72 ± 0.442
0.7ThrMet: 0.7 ± 0.106
4.603ThrAsn: 4.603 ± 0.433
3.452ThrPro: 3.452 ± 0.25
3.052ThrGln: 3.052 ± 0.209
2.335ThrArg: 2.335 ± 0.17
4.987ThrSer: 4.987 ± 0.418
6.387ThrThr: 6.387 ± 0.59
5.971ThrVal: 5.971 ± 0.497
0.7ThrTrp: 0.7 ± 0.122
2.969ThrTyr: 2.969 ± 0.228
0.0ThrXaa: 0.0 ± 0.0
Val
4.786ValAla: 4.786 ± 0.312
0.4ValCys: 0.4 ± 0.083
4.92ValAsp: 4.92 ± 0.289
4.453ValGlu: 4.453 ± 0.284
2.802ValPhe: 2.802 ± 0.215
5.12ValGly: 5.12 ± 0.381
0.65ValHis: 0.65 ± 0.106
3.919ValIle: 3.919 ± 0.274
3.802ValLys: 3.802 ± 0.264
4.87ValLeu: 4.87 ± 0.239
1.001ValMet: 1.001 ± 0.142
4.253ValAsn: 4.253 ± 0.266
2.652ValPro: 2.652 ± 0.306
2.335ValGln: 2.335 ± 0.213
2.752ValArg: 2.752 ± 0.226
5.404ValSer: 5.404 ± 0.414
6.287ValThr: 6.287 ± 0.448
5.303ValVal: 5.303 ± 0.367
0.484ValTrp: 0.484 ± 0.085
2.585ValTyr: 2.585 ± 0.201
0.0ValXaa: 0.0 ± 0.0
Trp
0.684TrpAla: 0.684 ± 0.109
0.05TrpCys: 0.05 ± 0.029
0.801TrpAsp: 0.801 ± 0.135
0.767TrpGlu: 0.767 ± 0.12
0.3TrpPhe: 0.3 ± 0.07
0.7TrpGly: 0.7 ± 0.115
0.334TrpHis: 0.334 ± 0.088
0.75TrpIle: 0.75 ± 0.113
0.834TrpLys: 0.834 ± 0.128
0.6TrpLeu: 0.6 ± 0.112
0.233TrpMet: 0.233 ± 0.069
0.734TrpAsn: 0.734 ± 0.121
0.083TrpPro: 0.083 ± 0.036
0.417TrpGln: 0.417 ± 0.092
0.567TrpArg: 0.567 ± 0.102
0.801TrpSer: 0.801 ± 0.125
0.75TrpThr: 0.75 ± 0.1
0.467TrpVal: 0.467 ± 0.108
0.117TrpTrp: 0.117 ± 0.048
0.584TrpTyr: 0.584 ± 0.099
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.168TyrAla: 2.168 ± 0.178
0.517TyrCys: 0.517 ± 0.113
3.352TyrAsp: 3.352 ± 0.254
2.835TyrGlu: 2.835 ± 0.259
2.235TyrPhe: 2.235 ± 0.199
2.352TyrGly: 2.352 ± 0.183
0.634TyrHis: 0.634 ± 0.117
2.518TyrIle: 2.518 ± 0.218
2.118TyrLys: 2.118 ± 0.22
3.352TyrLeu: 3.352 ± 0.275
0.734TyrMet: 0.734 ± 0.12
2.902TyrAsn: 2.902 ± 0.202
1.801TyrPro: 1.801 ± 0.162
1.851TyrGln: 1.851 ± 0.156
1.985TyrArg: 1.985 ± 0.183
2.602TyrSer: 2.602 ± 0.231
3.336TyrThr: 3.336 ± 0.25
2.969TyrVal: 2.969 ± 0.293
0.517TyrTrp: 0.517 ± 0.103
2.018TyrTyr: 2.018 ± 0.219
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 230 proteins (59962 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski