Nonomuraea longispora

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Nonomuraea

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8278 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R4NT00|A0A4R4NT00_9ACTN Aldehyde dehydrogenase OS=Nonomuraea longispora OX=1848320 GN=E1267_02345 PE=3 SV=1
MM1 pKa = 7.56ASDD4 pKa = 5.33GYY6 pKa = 11.07ALSWTLTGGNRR17 pKa = 11.84VVVEE21 pKa = 4.39IVAGADD27 pKa = 3.05ACADD31 pKa = 3.87CLVPLPVMEE40 pKa = 6.25AIMSDD45 pKa = 3.28ALEE48 pKa = 4.28PTPYY52 pKa = 10.26TLDD55 pKa = 3.19RR56 pKa = 11.84VVLPGGTT63 pKa = 3.28

Molecular weight: 6.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V2XJI6|A0A4V2XJI6_9ACTN Dihydrodipicolinate synthase family protein OS=Nonomuraea longispora OX=1848320 GN=E1267_28745 PE=4 SV=1
YYY2 pKa = 9.31KK3 pKa = 10.43QGRR6 pKa = 11.84RR7 pKa = 11.84LGRR10 pKa = 11.84GRR12 pKa = 11.84RR13 pKa = 11.84LGRR16 pKa = 11.84GRR18 pKa = 11.84RR19 pKa = 11.84LGRR22 pKa = 11.84GRR24 pKa = 11.84CLGGRR29 pKa = 11.84GRR31 pKa = 11.84LGGRR35 pKa = 11.84GRR37 pKa = 11.84GRR39 pKa = 11.84LGRR42 pKa = 11.84GRR44 pKa = 11.84RR45 pKa = 11.84LRR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84GTPSGRR55 pKa = 11.84RR56 pKa = 11.84GGGVRR61 pKa = 11.84GAGGRR66 pKa = 11.84CRR68 pKa = 11.84ARR70 pKa = 11.84LTGRR74 pKa = 11.84IRR76 pKa = 11.84HHH78 pKa = 5.94PLLHHH83 pKa = 5.59

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8278

0

8278

2640208

25

4019

318.9

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.082 ± 0.035

0.795 ± 0.008

5.807 ± 0.022

5.703 ± 0.026

2.854 ± 0.017

9.397 ± 0.027

2.283 ± 0.012

3.552 ± 0.019

2.086 ± 0.021

10.602 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.982 ± 0.01

1.755 ± 0.013

5.942 ± 0.022

2.677 ± 0.016

8.272 ± 0.026

4.987 ± 0.016

5.731 ± 0.022

8.806 ± 0.026

1.547 ± 0.011

2.139 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski