Aneurinibacillus danicus
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4349 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A511VD58|A0A511VD58_9BACL LexA_DNA_bind domain-containing protein OS=Aneurinibacillus danicus OX=267746 GN=ADA01nite_33380 PE=4 SV=1
MM1 pKa = 7.22 NVNGRR6 pKa = 11.84 LVEE9 pKa = 4.04 AAVVRR14 pKa = 11.84 VLFTVTLTVAIFDD27 pKa = 4.01 EE28 pKa = 4.72 TTGTEE33 pKa = 3.92 LCRR36 pKa = 11.84 FPVIVRR42 pKa = 11.84 FDD44 pKa = 3.98 DD45 pKa = 4.12 EE46 pKa = 5.22 FVLCVPEE53 pKa = 3.85 PLGRR57 pKa = 11.84 EE58 pKa = 4.03 NILCRR63 pKa = 11.84 ITAIDD68 pKa = 3.94 CTPTLNVLLGDD79 pKa = 4.06 MIEE82 pKa = 4.89 LEE84 pKa = 4.18 VTICKK89 pKa = 9.84 EE90 pKa = 3.79 IQVEE94 pKa = 4.37 AEE96 pKa = 4.08 VKK98 pKa = 10.91 LEE100 pKa = 3.91 VLAKK104 pKa = 9.8 FCQPRR109 pKa = 11.84 PIIPLPPEE117 pKa = 4.37 RR118 pKa = 11.84 GFEE121 pKa = 4.04 CPPFNFPPQCPDD133 pKa = 3.72 LFPVPNCDD141 pKa = 3.09 CQAVVNTLVTGATVDD156 pKa = 3.14 ILGVPEE162 pKa = 4.28 VGTVQLIADD171 pKa = 4.5 ICPNCNPGASTFSFTFFDD189 pKa = 4.0 TTPLPTTTPVPVPEE203 pKa = 4.59 PDD205 pKa = 3.17 IPGDD209 pKa = 3.28 QSFTLRR215 pKa = 11.84 PVSISPPTCLVQDD228 pKa = 3.45 IAGIVGALTATVTGTAVRR246 pKa = 11.84 TFTATGLQEE255 pKa = 4.12 QVSYY259 pKa = 10.16 TLTLVEE265 pKa = 4.59 NPVPGLPDD273 pKa = 3.74 AFILTLTDD281 pKa = 3.37 TSGVTIFSATVALVPDD297 pKa = 4.54 AEE299 pKa = 4.7 LTIQDD304 pKa = 4.51 CVTFPDD310 pKa = 3.95 VLNPPTIPP318 pKa = 3.63
Molecular weight: 34.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.884
IPC_protein 3.859
Toseland 3.656
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.139
Thurlkill 3.694
EMBOSS 3.757
Sillero 3.973
Patrickios 1.125
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.884
Protein with the highest isoelectric point:
>tr|A0A511VAZ1|A0A511VAZ1_9BACL Small acid-soluble spore protein OS=Aneurinibacillus danicus OX=267746 GN=ADA01nite_35450 PE=3 SV=1
MM1 pKa = 7.61 KK2 pKa = 8.8 PTFNPNNRR10 pKa = 11.84 KK11 pKa = 9.18 RR12 pKa = 11.84 KK13 pKa = 8.39 KK14 pKa = 8.69 VHH16 pKa = 5.46 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.19 NGRR28 pKa = 11.84 KK29 pKa = 8.86 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.705
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.427
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4349
0
4349
1227664
39
2820
282.3
31.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.19 ± 0.045
0.844 ± 0.013
4.75 ± 0.028
7.589 ± 0.041
4.065 ± 0.027
7.218 ± 0.034
2.142 ± 0.019
7.108 ± 0.033
6.071 ± 0.033
9.63 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.919 ± 0.019
3.651 ± 0.026
4.0 ± 0.025
3.812 ± 0.03
5.358 ± 0.029
5.51 ± 0.028
5.293 ± 0.024
7.357 ± 0.03
1.066 ± 0.013
3.426 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here