Hydrogenoanaerobacterium saccharovorans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Hydrogenoanaerobacterium

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2929 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7YNQ6|A0A1H7YNQ6_9FIRM Ferrous iron transport protein B OS=Hydrogenoanaerobacterium saccharovorans OX=474960 GN=SAMN05216180_0112 PE=4 SV=1
MM1 pKa = 7.59GLNALQYY8 pKa = 7.59TTQQGDD14 pKa = 3.91TFDD17 pKa = 3.96MLALDD22 pKa = 4.92AYY24 pKa = 10.45NDD26 pKa = 3.35EE27 pKa = 4.34GMAAVIINANPLYY40 pKa = 10.82AGVLVFDD47 pKa = 4.97AGVTLTIPIVEE58 pKa = 4.2ATSVQTLPPWKK69 pKa = 10.12RR70 pKa = 3.19

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7ZVJ8|A0A1H7ZVJ8_9FIRM DNA-binding transcriptional regulator FrmR family OS=Hydrogenoanaerobacterium saccharovorans OX=474960 GN=SAMN05216180_0928 PE=4 SV=1
MM1 pKa = 7.38EE2 pKa = 5.4RR3 pKa = 11.84NEE5 pKa = 3.92RR6 pKa = 11.84PEE8 pKa = 3.75RR9 pKa = 11.84GNRR12 pKa = 11.84MRR14 pKa = 11.84KK15 pKa = 8.82GRR17 pKa = 11.84KK18 pKa = 8.61KK19 pKa = 10.38VCAFCQRR26 pKa = 11.84KK27 pKa = 9.53AEE29 pKa = 4.4NISYY33 pKa = 10.81KK34 pKa = 10.66DD35 pKa = 3.37VAEE38 pKa = 3.94LRR40 pKa = 11.84KK41 pKa = 10.06YY42 pKa = 10.55LSEE45 pKa = 3.87RR46 pKa = 11.84AKK48 pKa = 10.08IVPRR52 pKa = 11.84RR53 pKa = 11.84VTGTCAHH60 pKa = 6.46HH61 pKa = 6.44QRR63 pKa = 11.84MLTVAIKK70 pKa = 10.29RR71 pKa = 11.84ARR73 pKa = 11.84HH74 pKa = 5.39LAMLPYY80 pKa = 10.31VSEE83 pKa = 4.2

Molecular weight: 9.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2929

0

2929

935315

29

4823

319.3

35.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.051 ± 0.053

1.564 ± 0.022

5.486 ± 0.038

6.353 ± 0.046

4.203 ± 0.034

6.916 ± 0.043

1.734 ± 0.019

7.641 ± 0.045

6.763 ± 0.04

9.172 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.875 ± 0.028

4.793 ± 0.036

3.561 ± 0.028

3.516 ± 0.027

3.926 ± 0.033

6.081 ± 0.038

5.74 ± 0.047

7.033 ± 0.037

0.825 ± 0.018

3.767 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski