Spinach latent virus
Average proteome isoelectric point is 7.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|O57162|O57162_9BROM RNA-directed RNA polymerase 2a OS=Spinach latent virus OX=42680 PE=4 SV=1
MM1 pKa = 7.46 DD2 pKa = 5.89 TIVQMKK8 pKa = 7.34 VLCYY12 pKa = 10.6 EE13 pKa = 4.13 SMILLMIPSDD23 pKa = 3.97 VNAPRR28 pKa = 11.84 PEE30 pKa = 3.95 TAIGRR35 pKa = 11.84 LISDD39 pKa = 4.03 AAVSGVLRR47 pKa = 11.84 QCDD50 pKa = 3.79 FTAAEE55 pKa = 4.35 ASQCFVPIITGRR67 pKa = 11.84 SEE69 pKa = 4.38 CIEE72 pKa = 3.91 TRR74 pKa = 11.84 FSGRR78 pKa = 11.84 EE79 pKa = 3.36 IQFRR83 pKa = 11.84 NFSYY87 pKa = 10.71 SSQSFAAAHH96 pKa = 5.94 RR97 pKa = 11.84 VCEE100 pKa = 3.53 ADD102 pKa = 3.43 YY103 pKa = 10.5 IYY105 pKa = 11.24 SRR107 pKa = 11.84 FQTRR111 pKa = 11.84 NTTIIDD117 pKa = 3.13 IGGNFCTHH125 pKa = 5.67 SKK127 pKa = 9.27 MGRR130 pKa = 11.84 DD131 pKa = 3.84 NVHH134 pKa = 5.96 SCCPILDD141 pKa = 3.72 VRR143 pKa = 11.84 DD144 pKa = 3.5 GARR147 pKa = 11.84 YY148 pKa = 7.17 TDD150 pKa = 3.1 RR151 pKa = 11.84 FMSVAGALEE160 pKa = 4.11 TQPDD164 pKa = 3.5 RR165 pKa = 11.84 EE166 pKa = 4.29 LRR168 pKa = 11.84 LNYY171 pKa = 9.89 CSNKK175 pKa = 9.76 FEE177 pKa = 5.1 DD178 pKa = 5.03 CDD180 pKa = 4.59 VSAPWAMAIHH190 pKa = 7.06 SISDD194 pKa = 3.36 IPITSVVKK202 pKa = 9.02 HH203 pKa = 5.74 CFRR206 pKa = 11.84 RR207 pKa = 11.84 GVKK210 pKa = 10.07 KK211 pKa = 10.81 LIASVMMDD219 pKa = 3.07 PMMLVASQGFMPRR232 pKa = 11.84 LNVQWEE238 pKa = 4.16 IEE240 pKa = 3.85 PDD242 pKa = 3.56 EE243 pKa = 4.3 EE244 pKa = 4.53 NKK246 pKa = 10.11 GSRR249 pKa = 11.84 RR250 pKa = 11.84 ISFHH254 pKa = 6.68 FVDD257 pKa = 5.61 APGLSYY263 pKa = 9.32 THH265 pKa = 6.8 NYY267 pKa = 9.12 DD268 pKa = 3.86 VLMQYY273 pKa = 7.39 MTCNQVIISGKK284 pKa = 8.37 AAYY287 pKa = 9.46 RR288 pKa = 11.84 VEE290 pKa = 4.2 RR291 pKa = 11.84 VADD294 pKa = 3.92 LSGVFIVEE302 pKa = 4.08 INPGGNCWNEE312 pKa = 3.9 RR313 pKa = 11.84 LDD315 pKa = 4.25 LVPMRR320 pKa = 11.84 DD321 pKa = 3.75 VSCAWMTSLKK331 pKa = 10.42 RR332 pKa = 11.84 KK333 pKa = 7.29 TLVRR337 pKa = 11.84 VAIPQYY343 pKa = 8.21 IHH345 pKa = 5.77 SWEE348 pKa = 4.18 VKK350 pKa = 10.13 YY351 pKa = 11.11 VIADD355 pKa = 3.34 TDD357 pKa = 3.65 FVRR360 pKa = 11.84 RR361 pKa = 11.84 VAEE364 pKa = 3.96 VSFRR368 pKa = 11.84 QYY370 pKa = 11.05 KK371 pKa = 10.35 PEE373 pKa = 4.09 TPLQDD378 pKa = 3.45 LVKK381 pKa = 10.61 SVATMISSASNHH393 pKa = 6.21 CIINGVTMQTGTPLAIEE410 pKa = 5.36 DD411 pKa = 4.34 YY412 pKa = 11.36 VPMAVTFVAFAKK424 pKa = 10.13 SRR426 pKa = 11.84 YY427 pKa = 9.52 DD428 pKa = 3.62 SIKK431 pKa = 10.46 EE432 pKa = 3.79 GMKK435 pKa = 9.35 MVRR438 pKa = 11.84 QRR440 pKa = 11.84 GTNIADD446 pKa = 3.89 PNDD449 pKa = 3.54 HH450 pKa = 6.89 YY451 pKa = 11.46 DD452 pKa = 3.65 YY453 pKa = 10.98 EE454 pKa = 5.63 KK455 pKa = 11.06 DD456 pKa = 3.51 GSDD459 pKa = 3.07 VKK461 pKa = 11.14 SFSNHH466 pKa = 6.48 IIPMKK471 pKa = 9.99 NTIKK475 pKa = 10.9 SLFFPMKK482 pKa = 10.59 VKK484 pKa = 10.6 MNDD487 pKa = 2.91 NSMLIYY493 pKa = 10.44 SQGILSTVVDD503 pKa = 4.88 EE504 pKa = 4.92 IKK506 pKa = 9.91 TLFGWDD512 pKa = 2.89 VWDD515 pKa = 4.04 TDD517 pKa = 3.84 DD518 pKa = 6.54 AVIQSLPSFYY528 pKa = 11.11 KK529 pKa = 9.97 MEE531 pKa = 4.99 DD532 pKa = 3.35 VFQVTSDD539 pKa = 4.15 HH540 pKa = 6.33 YY541 pKa = 10.73 CLSHH545 pKa = 6.15 TLSVDD550 pKa = 2.39 WWLEE554 pKa = 3.65 GLYY557 pKa = 10.53 DD558 pKa = 3.82 SYY560 pKa = 11.88 HH561 pKa = 7.01 DD562 pKa = 3.62 MRR564 pKa = 11.84 VAHH567 pKa = 6.72 KK568 pKa = 10.41 KK569 pKa = 10.51 KK570 pKa = 10.39 LDD572 pKa = 3.57 EE573 pKa = 4.4 EE574 pKa = 4.54 EE575 pKa = 4.06 SRR577 pKa = 11.84 KK578 pKa = 8.37 TKK580 pKa = 10.7 VEE582 pKa = 3.76 NALLKK587 pKa = 10.24 IAEE590 pKa = 4.38 VLEE593 pKa = 4.54 KK594 pKa = 10.5 PDD596 pKa = 3.66 VSDD599 pKa = 3.56 GLKK602 pKa = 10.54 ALSEE606 pKa = 4.12 LPIISSLLEE615 pKa = 4.11 KK616 pKa = 10.47 KK617 pKa = 10.48 SEE619 pKa = 4.29 EE620 pKa = 4.14 IVTKK624 pKa = 8.01 PQCRR628 pKa = 11.84 DD629 pKa = 2.99 TEE631 pKa = 4.24 KK632 pKa = 10.9 PHH634 pKa = 6.37 INPYY638 pKa = 10.75 ADD640 pKa = 4.6 AIKK643 pKa = 9.99 EE644 pKa = 3.9 AQAYY648 pKa = 8.93 YY649 pKa = 10.94 EE650 pKa = 4.04 EE651 pKa = 5.67 LEE653 pKa = 4.53 VVNTRR658 pKa = 11.84 NLRR661 pKa = 11.84 GVGDD665 pKa = 3.49 YY666 pKa = 10.85 LFWRR670 pKa = 11.84 KK671 pKa = 9.48 KK672 pKa = 10.32 SNYY675 pKa = 8.75 ASVWGADD682 pKa = 3.46 EE683 pKa = 4.25 SRR685 pKa = 11.84 VVLEE689 pKa = 3.47 PMARR693 pKa = 11.84 KK694 pKa = 9.55 FYY696 pKa = 11.01 SRR698 pKa = 11.84 DD699 pKa = 3.43 RR700 pKa = 11.84 NVSIPEE706 pKa = 4.01 YY707 pKa = 9.32 EE708 pKa = 4.07 RR709 pKa = 11.84 GMTVDD714 pKa = 4.15 GFVSIKK720 pKa = 9.94 WVDD723 pKa = 3.48 GNITEE728 pKa = 4.7 DD729 pKa = 2.89 TWKK732 pKa = 10.6 CLSKK736 pKa = 10.95 YY737 pKa = 10.72 AVVLLDD743 pKa = 3.48 SSCVFDD749 pKa = 4.06 AGSRR753 pKa = 11.84 IMPGLKK759 pKa = 9.69 KK760 pKa = 10.62 ALVMDD765 pKa = 4.95 ANFKK769 pKa = 10.43 IVIEE773 pKa = 4.87 DD774 pKa = 3.86 GVAGCGKK781 pKa = 6.92 TTSLLKK787 pKa = 9.87 QAKK790 pKa = 8.93 PDD792 pKa = 3.73 SDD794 pKa = 4.45 LLLAANRR801 pKa = 11.84 EE802 pKa = 4.15 TANDD806 pKa = 3.57 AKK808 pKa = 11.0 ASGVIPKK815 pKa = 10.09 ALEE818 pKa = 3.76 YY819 pKa = 10.19 RR820 pKa = 11.84 VRR822 pKa = 11.84 TVDD825 pKa = 3.05 SYY827 pKa = 12.31 LMLRR831 pKa = 11.84 TWFTAEE837 pKa = 3.69 RR838 pKa = 11.84 LLVDD842 pKa = 3.38 EE843 pKa = 5.37 CFLVHH848 pKa = 7.28 AGLVYY853 pKa = 10.47 AAATLARR860 pKa = 11.84 VKK862 pKa = 10.46 EE863 pKa = 4.43 VIAFGDD869 pKa = 3.67 TKK871 pKa = 10.74 QIPFVSRR878 pKa = 11.84 IPTVKK883 pKa = 9.94 LRR885 pKa = 11.84 HH886 pKa = 5.69 ASVVGTLKK894 pKa = 10.55 PRR896 pKa = 11.84 TITYY900 pKa = 8.44 RR901 pKa = 11.84 CPRR904 pKa = 11.84 DD905 pKa = 3.4 VTAVLSEE912 pKa = 4.12 KK913 pKa = 10.74 FYY915 pKa = 9.14 GTKK918 pKa = 10.35 VKK920 pKa = 9.79 TFNLVKK926 pKa = 10.61 QSLEE930 pKa = 3.95 LKK932 pKa = 10.49 NIDD935 pKa = 3.74 SSTEE939 pKa = 3.66 IPVEE943 pKa = 3.97 KK944 pKa = 9.58 DD945 pKa = 2.83 TLYY948 pKa = 10.43 IAHH951 pKa = 6.15 TQADD955 pKa = 3.55 KK956 pKa = 10.81 HH957 pKa = 7.55 ALMRR961 pKa = 11.84 LPGMNGIEE969 pKa = 3.97 VLTTHH974 pKa = 6.78 EE975 pKa = 4.37 AQGKK979 pKa = 7.0 TRR981 pKa = 11.84 DD982 pKa = 3.52 HH983 pKa = 6.77 VILVRR988 pKa = 11.84 LSKK991 pKa = 8.17 TTNLLYY997 pKa = 10.44 SGKK1000 pKa = 9.12 MPDD1003 pKa = 3.7 AGGSHH1008 pKa = 6.45 NLVGLSRR1015 pKa = 11.84 HH1016 pKa = 5.77 KK1017 pKa = 10.69 KK1018 pKa = 7.05 SLRR1021 pKa = 11.84 YY1022 pKa = 9.33 FSVFADD1028 pKa = 4.69 DD1029 pKa = 5.34 PDD1031 pKa = 3.92 DD1032 pKa = 4.48 QIATGIRR1039 pKa = 11.84 WSKK1042 pKa = 9.12 TLDD1045 pKa = 3.57 EE1046 pKa = 4.68 QEE1048 pKa = 3.99 LAAYY1052 pKa = 8.95 RR1053 pKa = 11.84 AAGG1056 pKa = 3.32
Molecular weight: 119.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.275
IPC2_protein 6.326
IPC_protein 6.376
Toseland 6.517
ProMoST 6.825
Dawson 6.781
Bjellqvist 6.795
Wikipedia 6.781
Rodwell 6.781
Grimsley 6.62
Solomon 6.781
Lehninger 6.781
Nozaki 7.088
DTASelect 7.19
Thurlkill 7.22
EMBOSS 7.19
Sillero 7.19
Patrickios 4.24
IPC_peptide 6.795
IPC2_peptide 6.883
IPC2.peptide.svr19 6.825
Protein with the highest isoelectric point:
>tr|O57165|O57165_9BROM Putative coat protein OS=Spinach latent virus OX=42680 PE=3 SV=1
MM1 pKa = 7.53 SGNAIEE7 pKa = 6.09 VNGQWYY13 pKa = 10.58 SPVTNNNAPSRR24 pKa = 11.84 GRR26 pKa = 11.84 GRR28 pKa = 11.84 GRR30 pKa = 11.84 GPTARR35 pKa = 11.84 SRR37 pKa = 11.84 NWAQSRR43 pKa = 11.84 ANAARR48 pKa = 11.84 SRR50 pKa = 11.84 PQTLVIGTMPTNLPTWKK67 pKa = 10.16 SFPGEE72 pKa = 3.75 QWHH75 pKa = 5.67 VVSGFSFPDD84 pKa = 2.7 RR85 pKa = 11.84 WNNGNIAYY93 pKa = 10.27 ASMKK97 pKa = 10.17 TEE99 pKa = 3.97 LGKK102 pKa = 10.43 IKK104 pKa = 9.39 TLHH107 pKa = 6.95 EE108 pKa = 3.83 ITKK111 pKa = 9.57 VYY113 pKa = 10.9 SVMYY117 pKa = 10.15 GFICKK122 pKa = 9.71 SDD124 pKa = 3.16 GHH126 pKa = 8.08 AGFITGFDD134 pKa = 3.93 TNNPTGPVAPDD145 pKa = 3.01 RR146 pKa = 11.84 VKK148 pKa = 10.85 VKK150 pKa = 10.68 KK151 pKa = 9.96 NAYY154 pKa = 8.68 CAKK157 pKa = 9.84 QQVFPAGTTVSEE169 pKa = 5.02 LKK171 pKa = 10.63 NDD173 pKa = 3.14 WSFIWEE179 pKa = 4.21 FDD181 pKa = 3.29 AAPVVGTEE189 pKa = 3.99 KK190 pKa = 10.56 QVTVTQFWVSTTPLPGVKK208 pKa = 9.66 PPSNFLVCEE217 pKa = 4.19 DD218 pKa = 3.64
Molecular weight: 24.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.02
IPC2_protein 8.99
IPC_protein 8.931
Toseland 9.75
ProMoST 9.414
Dawson 9.955
Bjellqvist 9.648
Wikipedia 10.101
Rodwell 10.379
Grimsley 10.014
Solomon 9.999
Lehninger 9.97
Nozaki 9.823
DTASelect 9.604
Thurlkill 9.809
EMBOSS 10.16
Sillero 9.897
Patrickios 9.823
IPC_peptide 9.999
IPC2_peptide 8.302
IPC2.peptide.svr19 7.862
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2548
195
1056
509.6
57.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.633 ± 0.534
2.119 ± 0.136
6.436 ± 0.679
5.024 ± 0.376
4.199 ± 0.347
5.102 ± 0.443
2.433 ± 0.383
5.416 ± 0.538
6.476 ± 0.194
8.203 ± 0.708
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.179 ± 0.228
4.16 ± 0.555
5.102 ± 0.693
3.1 ± 0.234
5.808 ± 0.114
7.535 ± 0.305
5.495 ± 0.49
8.399 ± 0.191
1.727 ± 0.286
3.454 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here