Plasmodium relictum
Average proteome isoelectric point is 7.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5136 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1J1H0G9|A0A1J1H0G9_PLARL Protein tyrosine phosphatase putative OS=Plasmodium relictum OX=85471 GN=PTP2 PE=4 SV=1
MM1 pKa = 7.66 LISVCISQEE10 pKa = 3.94 QEE12 pKa = 3.6 GNQYY16 pKa = 10.8 LFSEE20 pKa = 5.09 EE21 pKa = 3.95 ISSNVYY27 pKa = 8.27 ITEE30 pKa = 4.1 NSPNTGNLIDD40 pKa = 4.64 TINIEE45 pKa = 3.88 KK46 pKa = 10.88 DD47 pKa = 3.32 EE48 pKa = 4.3 ILDD51 pKa = 3.78 LEE53 pKa = 4.96 NIPSHH58 pKa = 6.97 FPIEE62 pKa = 4.37 EE63 pKa = 4.23 KK64 pKa = 11.04 NSDD67 pKa = 4.09 SDD69 pKa = 4.24 NKK71 pKa = 11.18 DD72 pKa = 3.22 DD73 pKa = 4.27 FFNMSLVDD81 pKa = 4.11 TINLEE86 pKa = 3.95 KK87 pKa = 10.83 SKK89 pKa = 10.95 SYY91 pKa = 10.44 DD92 pKa = 3.0 IKK94 pKa = 11.3 NVLDD98 pKa = 4.02 EE99 pKa = 4.52 VQCSLMDD106 pKa = 3.88 SLIDD110 pKa = 3.54 PFYY113 pKa = 10.75 PSFKK117 pKa = 10.29 SEE119 pKa = 4.18 KK120 pKa = 10.26 LPIHH124 pKa = 6.84 IEE126 pKa = 4.18 TEE128 pKa = 4.07 PSSLMEE134 pKa = 4.37 TKK136 pKa = 9.75 DD137 pKa = 3.51 TEE139 pKa = 4.14 HH140 pKa = 7.03 KK141 pKa = 10.96 YY142 pKa = 10.83 SVEE145 pKa = 4.0 LEE147 pKa = 4.25 NKK149 pKa = 10.19 LGEE152 pKa = 4.51 TPSTLPDD159 pKa = 3.54 SQTQPSNLPLEE170 pKa = 4.72 HH171 pKa = 7.1 EE172 pKa = 4.1 NSIIYY177 pKa = 10.1 IEE179 pKa = 4.4 TDD181 pKa = 3.31 PPSISLMGTKK191 pKa = 8.62 YY192 pKa = 9.19 TEE194 pKa = 3.46 HH195 pKa = 7.15 KK196 pKa = 10.74 YY197 pKa = 10.5 YY198 pKa = 11.11 VEE200 pKa = 4.21 LQDD203 pKa = 3.79 TLGEE207 pKa = 4.29 IPSTLPDD214 pKa = 3.37 SQTQSFNLLFEE225 pKa = 4.75 HH226 pKa = 7.67 DD227 pKa = 3.71 YY228 pKa = 11.18 AINVTHH234 pKa = 6.75 GKK236 pKa = 9.69 SFSVNNLMNTEE247 pKa = 3.97 NVEE250 pKa = 3.98 QEE252 pKa = 4.84 DD253 pKa = 4.13 PVEE256 pKa = 4.07 LQNILDD262 pKa = 4.86 EE263 pKa = 4.71 ILNTLPDD270 pKa = 4.12 SLTEE274 pKa = 4.83 PIDD277 pKa = 3.62 LLSEE281 pKa = 4.45 HH282 pKa = 6.84 EE283 pKa = 4.37 NSTIHH288 pKa = 7.35 AEE290 pKa = 4.11 AGPSNISLMGTEE302 pKa = 4.21 NEE304 pKa = 4.33 KK305 pKa = 11.23 HH306 pKa = 5.87 NDD308 pKa = 3.34 SYY310 pKa = 11.68 NPQYY314 pKa = 10.62 IFNYY318 pKa = 10.48 SLVTPNYY325 pKa = 10.28 LKK327 pKa = 11.03 EE328 pKa = 4.07 EE329 pKa = 4.03 FLNLLLEE336 pKa = 4.49 DD337 pKa = 3.58 QCPSINYY344 pKa = 9.63 EE345 pKa = 4.52 DD346 pKa = 4.05 IFPTTSNLMIPEE358 pKa = 4.0 NVEE361 pKa = 3.56 DD362 pKa = 3.72 EE363 pKa = 4.77 EE364 pKa = 4.6 YY365 pKa = 10.5 IKK367 pKa = 10.97 LQIIPVDD374 pKa = 4.08 FPIEE378 pKa = 4.1 KK379 pKa = 9.74 PATTINYY386 pKa = 9.16 NEE388 pKa = 4.63 DD389 pKa = 3.17 MSLLDD394 pKa = 4.95 EE395 pKa = 4.93 IEE397 pKa = 4.25 QISS400 pKa = 3.4
Molecular weight: 45.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.948
IPC_protein 3.935
Toseland 3.745
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.757
Rodwell 3.757
Grimsley 3.656
Solomon 3.884
Lehninger 3.834
Nozaki 3.986
DTASelect 4.139
Thurlkill 3.77
EMBOSS 3.77
Sillero 4.037
Patrickios 1.405
IPC_peptide 3.884
IPC2_peptide 4.024
IPC2.peptide.svr19 3.925
Protein with the highest isoelectric point:
>tr|A0A1J1HAA7|A0A1J1HAA7_PLARL Calponin homology domain-containing protein putative OS=Plasmodium relictum OX=85471 GN=PRELSG_1233200 PE=4 SV=1
MM1 pKa = 7.28 AHH3 pKa = 6.31 GASRR7 pKa = 11.84 YY8 pKa = 8.75 KK9 pKa = 10.48 KK10 pKa = 10.3 SRR12 pKa = 11.84 AKK14 pKa = 9.88 MRR16 pKa = 11.84 WKK18 pKa = 9.16 WKK20 pKa = 9.29 KK21 pKa = 9.72 KK22 pKa = 6.67 RR23 pKa = 11.84 TRR25 pKa = 11.84 RR26 pKa = 11.84 LQKK29 pKa = 9.69 KK30 pKa = 7.67 RR31 pKa = 11.84 RR32 pKa = 11.84 KK33 pKa = 8.24 MRR35 pKa = 11.84 QRR37 pKa = 11.84 SRR39 pKa = 3.22
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.436
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.676
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.486
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.193
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.032
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5136
0
5136
3624278
29
9310
705.7
83.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.851 ± 0.02
1.675 ± 0.012
5.483 ± 0.024
7.944 ± 0.044
5.24 ± 0.031
2.315 ± 0.022
1.728 ± 0.009
10.051 ± 0.035
13.073 ± 0.055
8.569 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.682 ± 0.01
13.802 ± 0.102
1.659 ± 0.019
2.208 ± 0.014
2.54 ± 0.016
7.133 ± 0.029
3.616 ± 0.018
3.388 ± 0.019
0.451 ± 0.008
5.578 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here