Salicola phage SCTP-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 541 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A248SKG2|A0A248SKG2_9CAUD Uncharacterized protein OS=Salicola phage SCTP-2 OX=2015814 GN=343 PE=4 SV=1
MM1 pKa = 7.9SDD3 pKa = 3.06TKK5 pKa = 11.17NYY7 pKa = 9.37GISGIGSNVEE17 pKa = 3.51LGKK20 pKa = 10.57RR21 pKa = 11.84GNRR24 pKa = 11.84FKK26 pKa = 11.52NEE28 pKa = 3.42TDD30 pKa = 3.52KK31 pKa = 11.43ISVRR35 pKa = 11.84DD36 pKa = 3.56FDD38 pKa = 4.56DD39 pKa = 4.61ANFVPISAGDD49 pKa = 3.65PVGDD53 pKa = 3.62NDD55 pKa = 5.82LVTKK59 pKa = 10.56SYY61 pKa = 11.38LEE63 pKa = 3.9QQAFDD68 pKa = 4.16PTLKK72 pKa = 11.01SPDD75 pKa = 4.54DD76 pKa = 4.29GDD78 pKa = 3.94LTDD81 pKa = 4.91GAVTDD86 pKa = 3.75WTVGSTTYY94 pKa = 9.61STAIDD99 pKa = 4.38DD100 pKa = 4.39LNGILGRR107 pKa = 11.84LVPTAPPEE115 pKa = 4.08LSTFTINATSGSNQLLAQNAIDD137 pKa = 3.8NTTNAPSAGSSVYY150 pKa = 10.18TVFDD154 pKa = 3.4SSFQSTQANGGNGAFGGDD172 pKa = 3.45VEE174 pKa = 4.89GADD177 pKa = 3.74GNLFLSGEE185 pKa = 4.26TGTLFSYY192 pKa = 11.58VNGTQDD198 pKa = 3.19GSIVLDD204 pKa = 3.76GTDD207 pKa = 3.13NTGTYY212 pKa = 10.61GSLEE216 pKa = 4.23VLSDD220 pKa = 3.17TAYY223 pKa = 9.97PADD226 pKa = 3.64TPGFYY231 pKa = 9.31TALKK235 pKa = 10.58ARR237 pKa = 11.84INKK240 pKa = 9.17SAVSIGYY247 pKa = 8.97NEE249 pKa = 4.9AYY251 pKa = 10.34LEE253 pKa = 4.17HH254 pKa = 7.17SEE256 pKa = 4.22TGSTNTVSWVYY267 pKa = 11.45DD268 pKa = 3.73DD269 pKa = 4.04VANAPSISNQSYY281 pKa = 10.43SLDD284 pKa = 3.62SAVSKK289 pKa = 10.68QSSGINHH296 pKa = 5.63MTDD299 pKa = 2.95GDD301 pKa = 4.24TLNVSATVDD310 pKa = 3.36NLVGQTYY317 pKa = 8.04ITGTVLRR324 pKa = 11.84FTTGVGVGTLNMDD337 pKa = 4.02ATQLGTGLTFPLDD350 pKa = 3.82ANLGPQTTSGKK361 pKa = 7.52TLSIASTATFDD372 pKa = 3.42EE373 pKa = 4.11TDD375 pKa = 3.19IQVRR379 pKa = 11.84AWNANGATGSSVVSGDD395 pKa = 3.27PVLVYY400 pKa = 9.5TSALEE405 pKa = 4.46GGNKK409 pKa = 8.73PHH411 pKa = 7.2EE412 pKa = 4.31SQINGSSADD421 pKa = 3.11AYY423 pKa = 10.48RR424 pKa = 11.84YY425 pKa = 9.78YY426 pKa = 10.61LGSGFTGDD434 pKa = 3.98TPSGTLDD441 pKa = 4.41APTTTNWDD449 pKa = 3.37STQLLNASGYY459 pKa = 6.56EE460 pKa = 4.05HH461 pKa = 7.17EE462 pKa = 4.86AVTVAGVVKK471 pKa = 10.51HH472 pKa = 6.11DD473 pKa = 3.56TTNYY477 pKa = 5.21TTGYY481 pKa = 10.17IPVTTGADD489 pKa = 3.52YY490 pKa = 9.42STKK493 pKa = 10.0GTDD496 pKa = 3.1QYY498 pKa = 10.23VTYY501 pKa = 10.83VFNQSTLSSISLDD514 pKa = 3.0ISGSYY519 pKa = 9.76SGLWVALPGISDD531 pKa = 4.19DD532 pKa = 5.52AGISPNALGGNWWDD546 pKa = 4.13AFEE549 pKa = 5.28LYY551 pKa = 10.26NGSGAPGRR559 pKa = 11.84TGDD562 pKa = 3.8SSAGCAVGSAASGSSGTVNLTFGTQSTTNSTNNAVIVRR600 pKa = 11.84IKK602 pKa = 10.87LNAGDD607 pKa = 4.75SISALSIGNTPP618 pKa = 3.26

Molecular weight: 63.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A248SK25|A0A248SK25_9CAUD Uncharacterized protein OS=Salicola phage SCTP-2 OX=2015814 GN=431 PE=4 SV=1
MM1 pKa = 7.7INTFKK6 pKa = 10.99CNSNEE11 pKa = 3.84WIHH14 pKa = 5.37NKK16 pKa = 9.93KK17 pKa = 9.3YY18 pKa = 10.45NSWRR22 pKa = 11.84HH23 pKa = 3.4RR24 pKa = 11.84TKK26 pKa = 10.67RR27 pKa = 11.84KK28 pKa = 9.81IIFNPILRR36 pKa = 11.84FVQGYY41 pKa = 7.8PFNKK45 pKa = 9.5RR46 pKa = 11.84PFIIASICDD55 pKa = 3.72NKK57 pKa = 10.65NGRR60 pKa = 11.84PFHH63 pKa = 6.25TGG65 pKa = 2.63

Molecular weight: 7.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

541

0

541

134053

19

3807

247.8

28.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.358 ± 0.095

1.05 ± 0.057

7.63 ± 0.124

7.256 ± 0.13

4.671 ± 0.107

4.519 ± 0.14

2.251 ± 0.075

8.658 ± 0.12

7.834 ± 0.21

7.165 ± 0.105

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.349 ± 0.086

8.839 ± 0.152

2.709 ± 0.064

3.5 ± 0.089

3.457 ± 0.088

7.214 ± 0.161

5.413 ± 0.154

5.407 ± 0.099

0.971 ± 0.043

5.751 ± 0.104
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski