Amino acid dipepetide frequency for Salicola phage SCTP-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.194AlaAla: 1.194 ± 0.093
0.291AlaCys: 0.291 ± 0.047
1.902AlaAsp: 1.902 ± 0.152
2.089AlaGlu: 2.089 ± 0.153
1.626AlaPhe: 1.626 ± 0.115
1.499AlaGly: 1.499 ± 0.133
0.761AlaHis: 0.761 ± 0.079
2.954AlaIle: 2.954 ± 0.138
2.372AlaLys: 2.372 ± 0.164
2.857AlaLeu: 2.857 ± 0.14
0.858AlaMet: 0.858 ± 0.088
2.29AlaAsn: 2.29 ± 0.131
0.873AlaPro: 0.873 ± 0.089
1.499AlaGln: 1.499 ± 0.121
1.365AlaArg: 1.365 ± 0.1
2.641AlaSer: 2.641 ± 0.147
1.88AlaThr: 1.88 ± 0.144
2.686AlaVal: 2.686 ± 0.21
0.321AlaTrp: 0.321 ± 0.045
1.619AlaTyr: 1.619 ± 0.114
0.0AlaXaa: 0.0 ± 0.0
Cys
0.313CysAla: 0.313 ± 0.044
0.179CysCys: 0.179 ± 0.049
0.791CysAsp: 0.791 ± 0.073
0.791CysGlu: 0.791 ± 0.084
0.612CysPhe: 0.612 ± 0.074
0.53CysGly: 0.53 ± 0.075
0.283CysHis: 0.283 ± 0.04
0.992CysIle: 0.992 ± 0.115
0.671CysLys: 0.671 ± 0.078
0.694CysLeu: 0.694 ± 0.083
0.239CysMet: 0.239 ± 0.039
0.739CysAsn: 0.739 ± 0.064
0.477CysPro: 0.477 ± 0.061
0.179CysGln: 0.179 ± 0.039
0.336CysArg: 0.336 ± 0.049
0.835CysSer: 0.835 ± 0.09
0.395CysThr: 0.395 ± 0.062
0.761CysVal: 0.761 ± 0.084
0.112CysTrp: 0.112 ± 0.03
0.574CysTyr: 0.574 ± 0.07
0.0CysXaa: 0.0 ± 0.0
Asp
2.342AspAla: 2.342 ± 0.172
0.627AspCys: 0.627 ± 0.084
5.789AspAsp: 5.789 ± 0.252
6.453AspGlu: 6.453 ± 0.274
3.543AspPhe: 3.543 ± 0.174
4.349AspGly: 4.349 ± 0.398
1.499AspHis: 1.499 ± 0.097
7.512AspIle: 7.512 ± 0.333
4.73AspLys: 4.73 ± 0.205
4.968AspLeu: 4.968 ± 0.231
1.79AspMet: 1.79 ± 0.113
6.773AspAsn: 6.773 ± 0.284
2.223AspPro: 2.223 ± 0.137
2.492AspGln: 2.492 ± 0.158
2.186AspArg: 2.186 ± 0.121
5.565AspSer: 5.565 ± 0.394
3.745AspThr: 3.745 ± 0.184
4.327AspVal: 4.327 ± 0.249
0.716AspTrp: 0.716 ± 0.08
4.662AspTyr: 4.662 ± 0.219
0.0AspXaa: 0.0 ± 0.0
Glu
2.432GluAla: 2.432 ± 0.199
0.776GluCys: 0.776 ± 0.084
5.475GluAsp: 5.475 ± 0.27
4.715GluGlu: 4.715 ± 0.317
3.7GluPhe: 3.7 ± 0.213
2.723GluGly: 2.723 ± 0.144
1.895GluHis: 1.895 ± 0.112
5.707GluIle: 5.707 ± 0.253
6.042GluLys: 6.042 ± 0.294
6.028GluLeu: 6.028 ± 0.224
1.738GluMet: 1.738 ± 0.146
6.781GluAsn: 6.781 ± 0.227
1.753GluPro: 1.753 ± 0.118
3.506GluGln: 3.506 ± 0.187
2.357GluArg: 2.357 ± 0.158
4.461GluSer: 4.461 ± 0.168
3.946GluThr: 3.946 ± 0.183
3.178GluVal: 3.178 ± 0.175
0.709GluTrp: 0.709 ± 0.087
4.64GluTyr: 4.64 ± 0.201
0.0GluXaa: 0.0 ± 0.0
Phe
1.231PheAla: 1.231 ± 0.09
0.433PheCys: 0.433 ± 0.063
3.76PheAsp: 3.76 ± 0.156
3.894PheGlu: 3.894 ± 0.195
2.134PhePhe: 2.134 ± 0.154
2.044PheGly: 2.044 ± 0.131
1.007PheHis: 1.007 ± 0.086
3.998PheIle: 3.998 ± 0.189
3.797PheLys: 3.797 ± 0.221
3.178PheLeu: 3.178 ± 0.194
1.164PheMet: 1.164 ± 0.101
4.282PheAsn: 4.282 ± 0.194
1.253PhePro: 1.253 ± 0.098
1.358PheGln: 1.358 ± 0.091
1.634PheArg: 1.634 ± 0.117
3.364PheSer: 3.364 ± 0.162
2.327PheThr: 2.327 ± 0.136
2.544PheVal: 2.544 ± 0.189
0.53PheTrp: 0.53 ± 0.066
2.775PheTyr: 2.775 ± 0.181
0.0PheXaa: 0.0 ± 0.0
Gly
1.484GlyAla: 1.484 ± 0.14
0.492GlyCys: 0.492 ± 0.066
3.708GlyAsp: 3.708 ± 0.225
3.379GlyGlu: 3.379 ± 0.155
2.178GlyPhe: 2.178 ± 0.11
2.238GlyGly: 2.238 ± 0.175
1.112GlyHis: 1.112 ± 0.107
3.215GlyIle: 3.215 ± 0.148
2.887GlyLys: 2.887 ± 0.143
3.029GlyLeu: 3.029 ± 0.16
0.977GlyMet: 0.977 ± 0.089
3.737GlyAsn: 3.737 ± 0.408
1.104GlyPro: 1.104 ± 0.2
1.492GlyGln: 1.492 ± 0.147
1.753GlyArg: 1.753 ± 0.139
3.969GlySer: 3.969 ± 0.344
2.566GlyThr: 2.566 ± 0.218
2.35GlyVal: 2.35 ± 0.139
0.649GlyTrp: 0.649 ± 0.077
2.872GlyTyr: 2.872 ± 0.182
0.0GlyXaa: 0.0 ± 0.0
His
0.597HisAla: 0.597 ± 0.063
0.336HisCys: 0.336 ± 0.055
1.567HisAsp: 1.567 ± 0.12
1.395HisGlu: 1.395 ± 0.104
1.141HisPhe: 1.141 ± 0.107
1.074HisGly: 1.074 ± 0.102
0.701HisHis: 0.701 ± 0.085
2.066HisIle: 2.066 ± 0.117
1.477HisLys: 1.477 ± 0.105
1.589HisLeu: 1.589 ± 0.109
0.433HisMet: 0.433 ± 0.059
2.096HisAsn: 2.096 ± 0.137
0.85HisPro: 0.85 ± 0.084
0.739HisGln: 0.739 ± 0.076
0.716HisArg: 0.716 ± 0.072
1.619HisSer: 1.619 ± 0.108
1.208HisThr: 1.208 ± 0.127
1.082HisVal: 1.082 ± 0.09
0.179HisTrp: 0.179 ± 0.04
1.641HisTyr: 1.641 ± 0.109
0.0HisXaa: 0.0 ± 0.0
Ile
3.111IleAla: 3.111 ± 0.145
0.768IleCys: 0.768 ± 0.086
7.81IleAsp: 7.81 ± 0.26
6.781IleGlu: 6.781 ± 0.306
3.335IlePhe: 3.335 ± 0.217
3.737IleGly: 3.737 ± 0.178
1.91IleHis: 1.91 ± 0.131
7.326IleIle: 7.326 ± 0.3
7.378IleLys: 7.378 ± 0.329
5.52IleLeu: 5.52 ± 0.252
1.984IleMet: 1.984 ± 0.115
8.28IleAsn: 8.28 ± 0.309
2.484IlePro: 2.484 ± 0.129
2.626IleGln: 2.626 ± 0.202
3.252IleArg: 3.252 ± 0.159
6.087IleSer: 6.087 ± 0.298
5.102IleThr: 5.102 ± 0.419
4.491IleVal: 4.491 ± 0.164
0.731IleTrp: 0.731 ± 0.113
3.864IleTyr: 3.864 ± 0.192
0.0IleXaa: 0.0 ± 0.0
Lys
2.842LysAla: 2.842 ± 0.169
0.686LysCys: 0.686 ± 0.091
5.714LysAsp: 5.714 ± 0.252
5.647LysGlu: 5.647 ± 0.323
3.126LysPhe: 3.126 ± 0.169
2.932LysGly: 2.932 ± 0.188
2.014LysHis: 2.014 ± 0.145
6.49LysIle: 6.49 ± 0.216
6.572LysLys: 6.572 ± 0.366
6.408LysLeu: 6.408 ± 0.249
2.029LysMet: 2.029 ± 0.156
7.378LysAsn: 7.378 ± 0.243
1.992LysPro: 1.992 ± 0.113
3.506LysGln: 3.506 ± 0.211
3.073LysArg: 3.073 ± 0.183
5.088LysSer: 5.088 ± 0.197
4.498LysThr: 4.498 ± 0.189
3.79LysVal: 3.79 ± 0.196
0.746LysTrp: 0.746 ± 0.072
3.812LysTyr: 3.812 ± 0.199
0.0LysXaa: 0.0 ± 0.0
Leu
2.365LeuAla: 2.365 ± 0.14
0.828LeuCys: 0.828 ± 0.094
5.543LeuAsp: 5.543 ± 0.249
5.341LeuGlu: 5.341 ± 0.213
3.357LeuPhe: 3.357 ± 0.186
3.118LeuGly: 3.118 ± 0.166
1.261LeuHis: 1.261 ± 0.108
5.326LeuIle: 5.326 ± 0.228
6.773LeuLys: 6.773 ± 0.296
5.132LeuLeu: 5.132 ± 0.233
1.708LeuMet: 1.708 ± 0.133
6.221LeuAsn: 6.221 ± 0.229
1.88LeuPro: 1.88 ± 0.14
2.439LeuGln: 2.439 ± 0.176
2.738LeuArg: 2.738 ± 0.145
5.498LeuSer: 5.498 ± 0.258
3.954LeuThr: 3.954 ± 0.184
3.23LeuVal: 3.23 ± 0.159
0.798LeuTrp: 0.798 ± 0.095
4.14LeuTyr: 4.14 ± 0.192
0.0LeuXaa: 0.0 ± 0.0
Met
1.067MetAla: 1.067 ± 0.094
0.179MetCys: 0.179 ± 0.031
1.693MetAsp: 1.693 ± 0.138
1.261MetGlu: 1.261 ± 0.107
1.298MetPhe: 1.298 ± 0.117
0.955MetGly: 0.955 ± 0.076
0.455MetHis: 0.455 ± 0.055
2.037MetIle: 2.037 ± 0.131
2.574MetLys: 2.574 ± 0.169
1.44MetLeu: 1.44 ± 0.114
0.537MetMet: 0.537 ± 0.06
2.245MetAsn: 2.245 ± 0.153
0.686MetPro: 0.686 ± 0.077
0.507MetGln: 0.507 ± 0.071
0.865MetArg: 0.865 ± 0.078
2.044MetSer: 2.044 ± 0.153
1.38MetThr: 1.38 ± 0.11
1.208MetVal: 1.208 ± 0.093
0.194MetTrp: 0.194 ± 0.037
0.865MetTyr: 0.865 ± 0.081
0.0MetXaa: 0.0 ± 0.0
Asn
2.842AsnAla: 2.842 ± 0.176
0.895AsnCys: 0.895 ± 0.099
6.803AsnAsp: 6.803 ± 0.265
6.468AsnGlu: 6.468 ± 0.216
3.476AsnPhe: 3.476 ± 0.182
4.498AsnGly: 4.498 ± 0.257
2.134AsnHis: 2.134 ± 0.143
8.579AsnIle: 8.579 ± 0.329
6.259AsnLys: 6.259 ± 0.247
5.52AsnLeu: 5.52 ± 0.21
1.753AsnMet: 1.753 ± 0.122
9.161AsnAsn: 9.161 ± 0.385
2.827AsnPro: 2.827 ± 0.185
2.589AsnGln: 2.589 ± 0.164
2.917AsnArg: 2.917 ± 0.17
5.975AsnSer: 5.975 ± 0.276
4.774AsnThr: 4.774 ± 0.184
5.14AsnVal: 5.14 ± 0.334
0.619AsnTrp: 0.619 ± 0.066
5.162AsnTyr: 5.162 ± 0.348
0.0AsnXaa: 0.0 ± 0.0
Pro
0.813ProAla: 0.813 ± 0.081
0.395ProCys: 0.395 ± 0.057
2.171ProAsp: 2.171 ± 0.172
2.566ProGlu: 2.566 ± 0.187
1.201ProPhe: 1.201 ± 0.102
0.806ProGly: 0.806 ± 0.086
0.671ProHis: 0.671 ± 0.071
2.148ProIle: 2.148 ± 0.152
1.828ProLys: 1.828 ± 0.141
1.768ProLeu: 1.768 ± 0.122
0.619ProMet: 0.619 ± 0.074
2.178ProAsn: 2.178 ± 0.129
0.612ProPro: 0.612 ± 0.065
0.895ProGln: 0.895 ± 0.081
0.761ProArg: 0.761 ± 0.086
2.447ProSer: 2.447 ± 0.164
1.671ProThr: 1.671 ± 0.116
1.492ProVal: 1.492 ± 0.132
0.313ProTrp: 0.313 ± 0.048
1.731ProTyr: 1.731 ± 0.128
0.0ProXaa: 0.0 ± 0.0
Gln
1.343GlnAla: 1.343 ± 0.105
0.44GlnCys: 0.44 ± 0.058
1.872GlnAsp: 1.872 ± 0.115
2.059GlnGlu: 2.059 ± 0.157
2.089GlnPhe: 2.089 ± 0.124
1.462GlnGly: 1.462 ± 0.102
0.821GlnHis: 0.821 ± 0.073
2.768GlnIle: 2.768 ± 0.155
2.574GlnLys: 2.574 ± 0.158
2.902GlnLeu: 2.902 ± 0.174
0.806GlnMet: 0.806 ± 0.07
2.745GlnAsn: 2.745 ± 0.129
0.843GlnPro: 0.843 ± 0.084
1.761GlnGln: 1.761 ± 0.121
1.373GlnArg: 1.373 ± 0.104
2.432GlnSer: 2.432 ± 0.144
1.887GlnThr: 1.887 ± 0.133
1.664GlnVal: 1.664 ± 0.122
0.44GlnTrp: 0.44 ± 0.081
2.723GlnTyr: 2.723 ± 0.153
0.0GlnXaa: 0.0 ± 0.0
Arg
1.059ArgAla: 1.059 ± 0.105
0.343ArgCys: 0.343 ± 0.054
2.148ArgAsp: 2.148 ± 0.122
2.193ArgGlu: 2.193 ± 0.129
2.089ArgPhe: 2.089 ± 0.135
1.388ArgGly: 1.388 ± 0.117
0.828ArgHis: 0.828 ± 0.089
3.148ArgIle: 3.148 ± 0.159
3.521ArgLys: 3.521 ± 0.19
2.745ArgLeu: 2.745 ± 0.155
0.992ArgMet: 0.992 ± 0.096
2.581ArgAsn: 2.581 ± 0.142
0.865ArgPro: 0.865 ± 0.096
1.335ArgGln: 1.335 ± 0.094
1.276ArgArg: 1.276 ± 0.105
2.134ArgSer: 2.134 ± 0.129
1.432ArgThr: 1.432 ± 0.101
1.962ArgVal: 1.962 ± 0.114
0.351ArgTrp: 0.351 ± 0.064
2.178ArgTyr: 2.178 ± 0.163
0.0ArgXaa: 0.0 ± 0.0
Ser
2.372SerAla: 2.372 ± 0.18
0.694SerCys: 0.694 ± 0.08
5.013SerAsp: 5.013 ± 0.241
5.408SerGlu: 5.408 ± 0.238
3.372SerPhe: 3.372 ± 0.164
3.916SerGly: 3.916 ± 0.365
1.32SerHis: 1.32 ± 0.097
6.259SerIle: 6.259 ± 0.271
6.072SerLys: 6.072 ± 0.269
4.849SerLeu: 4.849 ± 0.185
1.798SerMet: 1.798 ± 0.136
6.699SerAsn: 6.699 ± 0.492
1.716SerPro: 1.716 ± 0.15
2.581SerGln: 2.581 ± 0.177
1.932SerArg: 1.932 ± 0.149
5.908SerSer: 5.908 ± 0.508
3.894SerThr: 3.894 ± 0.32
3.849SerVal: 3.849 ± 0.265
0.582SerTrp: 0.582 ± 0.063
3.901SerTyr: 3.901 ± 0.173
0.0SerXaa: 0.0 ± 0.0
Thr
1.94ThrAla: 1.94 ± 0.136
0.388ThrCys: 0.388 ± 0.052
3.872ThrAsp: 3.872 ± 0.283
3.663ThrGlu: 3.663 ± 0.172
2.678ThrPhe: 2.678 ± 0.148
2.775ThrGly: 2.775 ± 0.193
1.156ThrHis: 1.156 ± 0.092
5.401ThrIle: 5.401 ± 0.514
3.596ThrLys: 3.596 ± 0.179
4.148ThrLeu: 4.148 ± 0.211
1.179ThrMet: 1.179 ± 0.093
4.319ThrAsn: 4.319 ± 0.25
1.611ThrPro: 1.611 ± 0.141
2.104ThrGln: 2.104 ± 0.15
1.581ThrArg: 1.581 ± 0.117
3.439ThrSer: 3.439 ± 0.157
2.991ThrThr: 2.991 ± 0.205
3.946ThrVal: 3.946 ± 0.348
0.343ThrTrp: 0.343 ± 0.055
2.999ThrTyr: 2.999 ± 0.238
0.0ThrXaa: 0.0 ± 0.0
Val
1.932ValAla: 1.932 ± 0.157
0.686ValCys: 0.686 ± 0.073
4.468ValAsp: 4.468 ± 0.251
4.095ValGlu: 4.095 ± 0.158
2.372ValPhe: 2.372 ± 0.12
2.134ValGly: 2.134 ± 0.158
1.082ValHis: 1.082 ± 0.1
4.431ValIle: 4.431 ± 0.185
4.521ValLys: 4.521 ± 0.213
3.984ValLeu: 3.984 ± 0.188
1.365ValMet: 1.365 ± 0.097
3.864ValAsn: 3.864 ± 0.184
1.365ValPro: 1.365 ± 0.113
1.596ValGln: 1.596 ± 0.099
2.283ValArg: 2.283 ± 0.16
4.043ValSer: 4.043 ± 0.381
2.947ValThr: 2.947 ± 0.272
2.991ValVal: 2.991 ± 0.179
0.597ValTrp: 0.597 ± 0.105
3.312ValTyr: 3.312 ± 0.209
0.0ValXaa: 0.0 ± 0.0
Trp
0.306TrpAla: 0.306 ± 0.05
0.201TrpCys: 0.201 ± 0.037
0.739TrpAsp: 0.739 ± 0.094
0.649TrpGlu: 0.649 ± 0.07
0.686TrpPhe: 0.686 ± 0.066
0.395TrpGly: 0.395 ± 0.062
0.186TrpHis: 0.186 ± 0.041
0.686TrpIle: 0.686 ± 0.081
0.828TrpLys: 0.828 ± 0.1
0.671TrpLeu: 0.671 ± 0.078
0.254TrpMet: 0.254 ± 0.045
0.858TrpAsn: 0.858 ± 0.088
0.119TrpPro: 0.119 ± 0.03
0.231TrpGln: 0.231 ± 0.065
0.358TrpArg: 0.358 ± 0.05
0.686TrpSer: 0.686 ± 0.11
0.53TrpThr: 0.53 ± 0.063
0.597TrpVal: 0.597 ± 0.071
0.142TrpTrp: 0.142 ± 0.036
0.589TrpTyr: 0.589 ± 0.067
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.992TyrAla: 1.992 ± 0.111
0.865TyrCys: 0.865 ± 0.083
5.461TyrAsp: 5.461 ± 0.351
3.715TyrGlu: 3.715 ± 0.205
2.693TyrPhe: 2.693 ± 0.139
2.618TyrGly: 2.618 ± 0.153
1.41TyrHis: 1.41 ± 0.113
5.535TyrIle: 5.535 ± 0.234
3.872TyrLys: 3.872 ± 0.186
4.222TyrLeu: 4.222 ± 0.205
1.261TyrMet: 1.261 ± 0.113
5.162TyrAsn: 5.162 ± 0.231
1.552TyrPro: 1.552 ± 0.141
1.567TyrGln: 1.567 ± 0.143
1.813TyrArg: 1.813 ± 0.135
3.901TyrSer: 3.901 ± 0.23
2.999TyrThr: 2.999 ± 0.274
2.783TyrVal: 2.783 ± 0.148
0.642TyrTrp: 0.642 ± 0.068
3.446TyrTyr: 3.446 ± 0.164
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 541 proteins (134053 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski