Lactobacillus phage SA-C12
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 121 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I9KK93|A0A1I9KK93_9CAUD Putative HNH homing endonuclease OS=Lactobacillus phage SA-C12 OX=1755697 GN=SAC12_064 PE=4 SV=1
MM1 pKa = 7.55 KK2 pKa = 10.19 FRR4 pKa = 11.84 VKK6 pKa = 9.84 MLPGDD11 pKa = 3.82 QYY13 pKa = 11.8 LEE15 pKa = 4.64 AYY17 pKa = 9.16 FGPDD21 pKa = 2.85 GKK23 pKa = 10.45 SVYY26 pKa = 9.78 MADD29 pKa = 3.62 KK30 pKa = 10.73 DD31 pKa = 4.33 CNKK34 pKa = 10.3 DD35 pKa = 3.56 FSDD38 pKa = 4.41 LGGPEE43 pKa = 3.82 YY44 pKa = 10.49 TQVFDD49 pKa = 3.6 EE50 pKa = 4.53 DD51 pKa = 4.5 TYY53 pKa = 11.29 FYY55 pKa = 9.8 YY56 pKa = 10.57 QYY58 pKa = 11.27 VFRR61 pKa = 11.84 DD62 pKa = 3.75 YY63 pKa = 11.31 LPEE66 pKa = 4.14 FNPNDD71 pKa = 3.22 KK72 pKa = 10.83 RR73 pKa = 11.84 FILVGDD79 pKa = 4.22 GEE81 pKa = 4.53 DD82 pKa = 3.15
Molecular weight: 9.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.862
IPC2_protein 4.139
IPC_protein 4.088
Toseland 3.872
ProMoST 4.177
Dawson 4.075
Bjellqvist 4.291
Wikipedia 4.037
Rodwell 3.91
Grimsley 3.783
Solomon 4.075
Lehninger 4.024
Nozaki 4.202
DTASelect 4.457
Thurlkill 3.935
EMBOSS 4.037
Sillero 4.202
Patrickios 1.977
IPC_peptide 4.062
IPC2_peptide 4.177
IPC2.peptide.svr19 4.119
Protein with the highest isoelectric point:
>tr|A0A1I9KK69|A0A1I9KK69_9CAUD Tail assembly chaperone OS=Lactobacillus phage SA-C12 OX=1755697 GN=SAC12_016 PE=4 SV=1
MM1 pKa = 7.47 LSCGRR6 pKa = 11.84 QMRR9 pKa = 11.84 HH10 pKa = 5.96 RR11 pKa = 11.84 DD12 pKa = 3.54 TPNGNSSQQAVLIPFPLAPYY32 pKa = 9.25 TILPKK37 pKa = 8.44 WWKK40 pKa = 9.44 YY41 pKa = 6.37 YY42 pKa = 10.83 HH43 pKa = 6.52 NFAFFGRR50 pKa = 11.84 ISTTSKK56 pKa = 10.89 KK57 pKa = 10.55 FFFLLLTILKK67 pKa = 10.12 LYY69 pKa = 10.51 DD70 pKa = 3.17 ILFSIGGIKK79 pKa = 10.28 NGQTLSKK86 pKa = 10.92 AA87 pKa = 3.58
Molecular weight: 10.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.257
IPC2_protein 9.882
IPC_protein 10.101
Toseland 10.438
ProMoST 10.058
Dawson 10.584
Bjellqvist 10.248
Wikipedia 10.745
Rodwell 11.14
Grimsley 10.643
Solomon 10.613
Lehninger 10.599
Nozaki 10.409
DTASelect 10.233
Thurlkill 10.452
EMBOSS 10.818
Sillero 10.496
Patrickios 10.906
IPC_peptide 10.613
IPC2_peptide 9.019
IPC2.peptide.svr19 8.605
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
121
0
121
23661
43
1886
195.5
21.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.145 ± 0.56
0.879 ± 0.134
7.058 ± 0.44
5.507 ± 0.341
3.918 ± 0.185
6.847 ± 0.483
1.771 ± 0.125
6.948 ± 0.202
8.14 ± 0.365
7.785 ± 0.244
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.654 ± 0.135
6.475 ± 0.233
2.603 ± 0.159
3.559 ± 0.215
3.495 ± 0.176
7.789 ± 0.366
6.052 ± 0.386
6.542 ± 0.241
1.259 ± 0.129
4.573 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here