Amino acid dipepetide frequency for Lactobacillus phage SA-C12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.747AlaAla: 2.747 ± 0.807
0.465AlaCys: 0.465 ± 0.2
4.353AlaAsp: 4.353 ± 0.5
2.409AlaGlu: 2.409 ± 0.281
2.071AlaPhe: 2.071 ± 0.34
4.691AlaGly: 4.691 ± 0.799
0.845AlaHis: 0.845 ± 0.231
4.649AlaIle: 4.649 ± 0.521
5.875AlaLys: 5.875 ± 0.818
5.114AlaLeu: 5.114 ± 0.748
1.606AlaMet: 1.606 ± 0.319
3.254AlaAsn: 3.254 ± 0.484
1.057AlaPro: 1.057 ± 0.197
2.325AlaGln: 2.325 ± 0.44
1.902AlaArg: 1.902 ± 0.237
4.818AlaSer: 4.818 ± 0.926
4.353AlaThr: 4.353 ± 0.717
4.903AlaVal: 4.903 ± 0.481
0.845AlaTrp: 0.845 ± 0.187
3.17AlaTyr: 3.17 ± 0.484
0.0AlaXaa: 0.0 ± 0.0
Cys
0.507CysAla: 0.507 ± 0.155
0.169CysCys: 0.169 ± 0.072
0.338CysAsp: 0.338 ± 0.114
0.211CysGlu: 0.211 ± 0.096
0.634CysPhe: 0.634 ± 0.194
0.803CysGly: 0.803 ± 0.204
0.169CysHis: 0.169 ± 0.086
0.423CysIle: 0.423 ± 0.154
0.465CysLys: 0.465 ± 0.145
0.888CysLeu: 0.888 ± 0.232
0.211CysMet: 0.211 ± 0.138
0.338CysAsn: 0.338 ± 0.107
0.338CysPro: 0.338 ± 0.143
0.423CysGln: 0.423 ± 0.132
0.338CysArg: 0.338 ± 0.136
0.592CysSer: 0.592 ± 0.171
0.338CysThr: 0.338 ± 0.167
0.761CysVal: 0.761 ± 0.221
0.254CysTrp: 0.254 ± 0.117
0.592CysTyr: 0.592 ± 0.189
0.0CysXaa: 0.0 ± 0.0
Asp
3.804AspAla: 3.804 ± 0.455
0.676AspCys: 0.676 ± 0.14
5.833AspAsp: 5.833 ± 0.949
4.987AspGlu: 4.987 ± 0.512
3.466AspPhe: 3.466 ± 0.459
5.959AspGly: 5.959 ± 0.622
1.395AspHis: 1.395 ± 0.275
4.142AspIle: 4.142 ± 0.459
6.002AspLys: 6.002 ± 0.709
5.664AspLeu: 5.664 ± 0.565
1.986AspMet: 1.986 ± 0.298
4.1AspAsn: 4.1 ± 0.479
1.691AspPro: 1.691 ± 0.3
2.029AspGln: 2.029 ± 0.369
2.24AspArg: 2.24 ± 0.317
4.776AspSer: 4.776 ± 0.467
3.423AspThr: 3.423 ± 0.488
4.269AspVal: 4.269 ± 0.42
1.226AspTrp: 1.226 ± 0.233
2.916AspTyr: 2.916 ± 0.423
0.0AspXaa: 0.0 ± 0.0
Glu
3.085GluAla: 3.085 ± 0.371
0.719GluCys: 0.719 ± 0.179
3.762GluAsp: 3.762 ± 0.527
3.297GluGlu: 3.297 ± 0.507
2.113GluPhe: 2.113 ± 0.363
1.733GluGly: 1.733 ± 0.226
0.803GluHis: 0.803 ± 0.156
4.565GluIle: 4.565 ± 0.448
4.565GluLys: 4.565 ± 0.433
5.621GluLeu: 5.621 ± 0.503
1.986GluMet: 1.986 ± 0.254
3.888GluAsn: 3.888 ± 0.458
1.352GluPro: 1.352 ± 0.284
1.986GluGln: 1.986 ± 0.304
2.367GluArg: 2.367 ± 0.368
3.128GluSer: 3.128 ± 0.314
3.085GluThr: 3.085 ± 0.357
3.297GluVal: 3.297 ± 0.467
0.845GluTrp: 0.845 ± 0.175
2.874GluTyr: 2.874 ± 0.354
0.0GluXaa: 0.0 ± 0.0
Phe
1.648PheAla: 1.648 ± 0.25
0.761PheCys: 0.761 ± 0.224
2.959PheAsp: 2.959 ± 0.377
1.817PheGlu: 1.817 ± 0.325
1.902PhePhe: 1.902 ± 0.355
3.254PheGly: 3.254 ± 0.347
0.465PheHis: 0.465 ± 0.139
1.944PheIle: 1.944 ± 0.306
3.466PheLys: 3.466 ± 0.399
2.62PheLeu: 2.62 ± 0.446
0.888PheMet: 0.888 ± 0.168
2.79PheAsn: 2.79 ± 0.418
1.352PhePro: 1.352 ± 0.254
1.141PheGln: 1.141 ± 0.207
1.31PheArg: 1.31 ± 0.229
3.466PheSer: 3.466 ± 0.482
2.409PheThr: 2.409 ± 0.271
2.747PheVal: 2.747 ± 0.468
0.38PheTrp: 0.38 ± 0.116
1.86PheTyr: 1.86 ± 0.284
0.0PheXaa: 0.0 ± 0.0
Gly
3.931GlyAla: 3.931 ± 0.638
0.211GlyCys: 0.211 ± 0.101
5.241GlyAsp: 5.241 ± 0.827
3.128GlyGlu: 3.128 ± 0.441
2.79GlyPhe: 2.79 ± 0.382
4.057GlyGly: 4.057 ± 0.862
1.31GlyHis: 1.31 ± 0.266
4.522GlyIle: 4.522 ± 0.654
6.805GlyLys: 6.805 ± 1.222
5.833GlyLeu: 5.833 ± 0.454
1.902GlyMet: 1.902 ± 0.279
5.03GlyAsn: 5.03 ± 0.552
0.845GlyPro: 0.845 ± 0.181
2.029GlyGln: 2.029 ± 0.353
2.79GlyArg: 2.79 ± 0.379
4.818GlySer: 4.818 ± 0.51
4.691GlyThr: 4.691 ± 0.599
4.1GlyVal: 4.1 ± 0.399
0.972GlyTrp: 0.972 ± 0.19
3.466GlyTyr: 3.466 ± 0.34
0.0GlyXaa: 0.0 ± 0.0
His
0.761HisAla: 0.761 ± 0.159
0.085HisCys: 0.085 ± 0.061
1.268HisAsp: 1.268 ± 0.22
1.099HisGlu: 1.099 ± 0.223
0.803HisPhe: 0.803 ± 0.201
1.522HisGly: 1.522 ± 0.241
0.465HisHis: 0.465 ± 0.169
1.183HisIle: 1.183 ± 0.231
1.31HisLys: 1.31 ± 0.236
1.014HisLeu: 1.014 ± 0.202
0.465HisMet: 0.465 ± 0.14
1.226HisAsn: 1.226 ± 0.262
0.549HisPro: 0.549 ± 0.156
0.592HisGln: 0.592 ± 0.154
0.761HisArg: 0.761 ± 0.181
1.352HisSer: 1.352 ± 0.272
1.099HisThr: 1.099 ± 0.221
1.183HisVal: 1.183 ± 0.245
0.169HisTrp: 0.169 ± 0.075
0.803HisTyr: 0.803 ± 0.223
0.0HisXaa: 0.0 ± 0.0
Ile
4.945IleAla: 4.945 ± 0.557
0.549IleCys: 0.549 ± 0.183
5.537IleAsp: 5.537 ± 0.447
3.931IleGlu: 3.931 ± 0.437
2.198IlePhe: 2.198 ± 0.407
5.241IleGly: 5.241 ± 0.989
1.014IleHis: 1.014 ± 0.207
4.353IleIle: 4.353 ± 0.576
5.706IleLys: 5.706 ± 0.586
4.396IleLeu: 4.396 ± 0.42
1.775IleMet: 1.775 ± 0.293
5.748IleAsn: 5.748 ± 0.475
2.198IlePro: 2.198 ± 0.344
1.986IleGln: 1.986 ± 0.304
2.113IleArg: 2.113 ± 0.304
5.664IleSer: 5.664 ± 0.469
4.353IleThr: 4.353 ± 0.465
4.48IleVal: 4.48 ± 0.444
0.592IleTrp: 0.592 ± 0.163
2.705IleTyr: 2.705 ± 0.313
0.0IleXaa: 0.0 ± 0.0
Lys
4.227LysAla: 4.227 ± 0.704
0.38LysCys: 0.38 ± 0.13
5.41LysAsp: 5.41 ± 0.54
4.818LysGlu: 4.818 ± 0.531
2.832LysPhe: 2.832 ± 0.316
4.734LysGly: 4.734 ± 0.826
1.479LysHis: 1.479 ± 0.265
6.213LysIle: 6.213 ± 0.699
6.509LysLys: 6.509 ± 0.592
6.762LysLeu: 6.762 ± 0.483
2.325LysMet: 2.325 ± 0.461
5.706LysAsn: 5.706 ± 0.536
2.113LysPro: 2.113 ± 0.32
3.128LysGln: 3.128 ± 0.362
3.381LysArg: 3.381 ± 0.336
5.114LysSer: 5.114 ± 0.683
5.41LysThr: 5.41 ± 0.446
5.452LysVal: 5.452 ± 0.674
1.352LysTrp: 1.352 ± 0.335
4.057LysTyr: 4.057 ± 0.479
0.0LysXaa: 0.0 ± 0.0
Leu
4.861LeuAla: 4.861 ± 0.585
0.719LeuCys: 0.719 ± 0.178
5.621LeuAsp: 5.621 ± 0.555
4.945LeuGlu: 4.945 ± 0.504
3.128LeuPhe: 3.128 ± 0.445
4.565LeuGly: 4.565 ± 0.527
1.352LeuHis: 1.352 ± 0.257
5.537LeuIle: 5.537 ± 0.546
5.748LeuLys: 5.748 ± 0.535
5.579LeuLeu: 5.579 ± 0.464
2.24LeuMet: 2.24 ± 0.415
4.522LeuAsn: 4.522 ± 0.412
2.536LeuPro: 2.536 ± 0.248
2.578LeuGln: 2.578 ± 0.283
3.085LeuArg: 3.085 ± 0.347
7.566LeuSer: 7.566 ± 0.492
4.522LeuThr: 4.522 ± 0.508
5.241LeuVal: 5.241 ± 0.456
0.719LeuTrp: 0.719 ± 0.178
2.79LeuTyr: 2.79 ± 0.443
0.0LeuXaa: 0.0 ± 0.0
Met
1.775MetAla: 1.775 ± 0.286
0.465MetCys: 0.465 ± 0.213
0.93MetAsp: 0.93 ± 0.167
1.564MetGlu: 1.564 ± 0.269
1.099MetPhe: 1.099 ± 0.205
1.141MetGly: 1.141 ± 0.224
0.338MetHis: 0.338 ± 0.129
2.282MetIle: 2.282 ± 0.317
2.24MetLys: 2.24 ± 0.234
2.832MetLeu: 2.832 ± 0.34
0.592MetMet: 0.592 ± 0.141
1.564MetAsn: 1.564 ± 0.291
0.549MetPro: 0.549 ± 0.139
1.057MetGln: 1.057 ± 0.171
1.014MetArg: 1.014 ± 0.199
2.494MetSer: 2.494 ± 0.303
2.029MetThr: 2.029 ± 0.217
1.817MetVal: 1.817 ± 0.338
0.085MetTrp: 0.085 ± 0.059
0.676MetTyr: 0.676 ± 0.204
0.0MetXaa: 0.0 ± 0.0
Asn
4.142AsnAla: 4.142 ± 0.349
0.507AsnCys: 0.507 ± 0.146
3.593AsnAsp: 3.593 ± 0.358
3.762AsnGlu: 3.762 ± 0.466
2.62AsnPhe: 2.62 ± 0.298
6.467AsnGly: 6.467 ± 0.759
1.099AsnHis: 1.099 ± 0.225
4.945AsnIle: 4.945 ± 0.456
5.241AsnLys: 5.241 ± 0.402
4.015AsnLeu: 4.015 ± 0.413
1.902AsnMet: 1.902 ± 0.257
4.396AsnAsn: 4.396 ± 0.435
2.198AsnPro: 2.198 ± 0.282
2.282AsnGln: 2.282 ± 0.337
1.902AsnArg: 1.902 ± 0.296
4.522AsnSer: 4.522 ± 0.682
3.466AsnThr: 3.466 ± 0.434
3.719AsnVal: 3.719 ± 0.391
0.972AsnTrp: 0.972 ± 0.207
3.001AsnTyr: 3.001 ± 0.413
0.0AsnXaa: 0.0 ± 0.0
Pro
1.437ProAla: 1.437 ± 0.304
0.127ProCys: 0.127 ± 0.091
2.409ProAsp: 2.409 ± 0.327
2.282ProGlu: 2.282 ± 0.374
0.592ProPhe: 0.592 ± 0.152
1.141ProGly: 1.141 ± 0.196
0.423ProHis: 0.423 ± 0.125
1.902ProIle: 1.902 ± 0.325
1.817ProLys: 1.817 ± 0.253
1.775ProLeu: 1.775 ± 0.289
0.465ProMet: 0.465 ± 0.138
1.606ProAsn: 1.606 ± 0.243
0.338ProPro: 0.338 ± 0.137
1.141ProGln: 1.141 ± 0.247
0.676ProArg: 0.676 ± 0.212
1.986ProSer: 1.986 ± 0.26
1.733ProThr: 1.733 ± 0.305
2.367ProVal: 2.367 ± 0.305
0.211ProTrp: 0.211 ± 0.092
1.606ProTyr: 1.606 ± 0.239
0.0ProXaa: 0.0 ± 0.0
Gln
3.128GlnAla: 3.128 ± 0.648
0.296GlnCys: 0.296 ± 0.109
1.775GlnAsp: 1.775 ± 0.236
1.986GlnGlu: 1.986 ± 0.308
1.395GlnPhe: 1.395 ± 0.265
1.902GlnGly: 1.902 ± 0.338
0.888GlnHis: 0.888 ± 0.206
3.128GlnIle: 3.128 ± 0.44
2.536GlnLys: 2.536 ± 0.343
2.663GlnLeu: 2.663 ± 0.295
0.803GlnMet: 0.803 ± 0.145
1.817GlnAsn: 1.817 ± 0.302
0.888GlnPro: 0.888 ± 0.197
1.395GlnGln: 1.395 ± 0.216
1.183GlnArg: 1.183 ± 0.246
2.663GlnSer: 2.663 ± 0.356
3.339GlnThr: 3.339 ± 0.959
1.691GlnVal: 1.691 ± 0.228
0.254GlnTrp: 0.254 ± 0.09
1.86GlnTyr: 1.86 ± 0.256
0.0GlnXaa: 0.0 ± 0.0
Arg
2.071ArgAla: 2.071 ± 0.297
0.254ArgCys: 0.254 ± 0.11
2.578ArgAsp: 2.578 ± 0.426
2.198ArgGlu: 2.198 ± 0.332
1.352ArgPhe: 1.352 ± 0.237
1.944ArgGly: 1.944 ± 0.277
0.719ArgHis: 0.719 ± 0.214
2.156ArgIle: 2.156 ± 0.324
3.001ArgLys: 3.001 ± 0.404
3.128ArgLeu: 3.128 ± 0.393
0.972ArgMet: 0.972 ± 0.191
1.986ArgAsn: 1.986 ± 0.26
0.93ArgPro: 0.93 ± 0.192
0.888ArgGln: 0.888 ± 0.2
1.31ArgArg: 1.31 ± 0.207
2.79ArgSer: 2.79 ± 0.364
1.775ArgThr: 1.775 ± 0.268
2.578ArgVal: 2.578 ± 0.386
0.465ArgTrp: 0.465 ± 0.176
1.86ArgTyr: 1.86 ± 0.325
0.0ArgXaa: 0.0 ± 0.0
Ser
5.241SerAla: 5.241 ± 0.868
0.338SerCys: 0.338 ± 0.104
5.495SerAsp: 5.495 ± 0.626
4.48SerGlu: 4.48 ± 0.357
2.959SerPhe: 2.959 ± 0.333
5.706SerGly: 5.706 ± 0.607
1.691SerHis: 1.691 ± 0.263
5.03SerIle: 5.03 ± 0.513
5.706SerLys: 5.706 ± 0.622
5.452SerLeu: 5.452 ± 0.501
1.817SerMet: 1.817 ± 0.222
5.241SerAsn: 5.241 ± 0.462
2.198SerPro: 2.198 ± 0.284
3.635SerGln: 3.635 ± 0.776
2.409SerArg: 2.409 ± 0.397
7.016SerSer: 7.016 ± 0.905
4.057SerThr: 4.057 ± 0.59
5.199SerVal: 5.199 ± 0.459
0.972SerTrp: 0.972 ± 0.191
3.254SerTyr: 3.254 ± 0.445
0.0SerXaa: 0.0 ± 0.0
Thr
5.114ThrAla: 5.114 ± 1.274
0.254ThrCys: 0.254 ± 0.121
4.311ThrAsp: 4.311 ± 0.374
2.451ThrGlu: 2.451 ± 0.31
2.325ThrPhe: 2.325 ± 0.284
4.142ThrGly: 4.142 ± 0.465
1.057ThrHis: 1.057 ± 0.243
4.945ThrIle: 4.945 ± 0.399
4.142ThrLys: 4.142 ± 0.406
4.987ThrLeu: 4.987 ± 0.472
1.352ThrMet: 1.352 ± 0.244
3.17ThrAsn: 3.17 ± 0.369
1.691ThrPro: 1.691 ± 0.222
2.451ThrGln: 2.451 ± 0.587
1.902ThrArg: 1.902 ± 0.318
5.03ThrSer: 5.03 ± 0.543
3.339ThrThr: 3.339 ± 0.505
4.269ThrVal: 4.269 ± 0.489
0.423ThrTrp: 0.423 ± 0.124
3.17ThrTyr: 3.17 ± 0.64
0.0ThrXaa: 0.0 ± 0.0
Val
4.227ValAla: 4.227 ± 0.487
0.761ValCys: 0.761 ± 0.228
4.227ValAsp: 4.227 ± 0.534
3.423ValGlu: 3.423 ± 0.349
2.367ValPhe: 2.367 ± 0.298
5.114ValGly: 5.114 ± 0.515
0.93ValHis: 0.93 ± 0.192
4.227ValIle: 4.227 ± 0.544
6.044ValLys: 6.044 ± 0.45
5.072ValLeu: 5.072 ± 0.597
1.691ValMet: 1.691 ± 0.336
4.861ValAsn: 4.861 ± 0.428
1.733ValPro: 1.733 ± 0.272
1.902ValGln: 1.902 ± 0.305
1.86ValArg: 1.86 ± 0.286
5.917ValSer: 5.917 ± 0.369
3.762ValThr: 3.762 ± 0.435
4.015ValVal: 4.015 ± 0.467
0.803ValTrp: 0.803 ± 0.165
2.451ValTyr: 2.451 ± 0.385
0.0ValXaa: 0.0 ± 0.0
Trp
0.719TrpAla: 0.719 ± 0.153
0.211TrpCys: 0.211 ± 0.092
0.845TrpAsp: 0.845 ± 0.208
0.592TrpGlu: 0.592 ± 0.148
1.099TrpPhe: 1.099 ± 0.268
0.803TrpGly: 0.803 ± 0.153
0.211TrpHis: 0.211 ± 0.089
0.845TrpIle: 0.845 ± 0.196
0.803TrpLys: 0.803 ± 0.221
1.141TrpLeu: 1.141 ± 0.27
0.296TrpMet: 0.296 ± 0.113
0.803TrpAsn: 0.803 ± 0.204
0.169TrpPro: 0.169 ± 0.08
0.507TrpGln: 0.507 ± 0.128
0.507TrpArg: 0.507 ± 0.152
0.845TrpSer: 0.845 ± 0.202
0.719TrpThr: 0.719 ± 0.155
0.719TrpVal: 0.719 ± 0.171
0.296TrpTrp: 0.296 ± 0.105
0.465TrpTyr: 0.465 ± 0.131
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.085TyrAla: 3.085 ± 0.331
0.845TyrCys: 0.845 ± 0.233
4.1TyrAsp: 4.1 ± 0.628
1.691TyrGlu: 1.691 ± 0.286
1.437TyrPhe: 1.437 ± 0.363
3.55TyrGly: 3.55 ± 0.478
1.057TyrHis: 1.057 ± 0.2
2.494TyrIle: 2.494 ± 0.4
3.423TyrLys: 3.423 ± 0.444
3.297TyrLeu: 3.297 ± 0.499
1.226TyrMet: 1.226 ± 0.223
2.705TyrAsn: 2.705 ± 0.368
1.31TyrPro: 1.31 ± 0.202
2.113TyrGln: 2.113 ± 0.511
1.902TyrArg: 1.902 ± 0.302
3.339TyrSer: 3.339 ± 0.451
2.62TyrThr: 2.62 ± 0.347
2.62TyrVal: 2.62 ± 0.431
0.761TyrTrp: 0.761 ± 0.151
2.156TyrTyr: 2.156 ± 0.339
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 121 proteins (23661 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski