Phyllobacterium endophyticum
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5125 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P7B191|A0A2P7B191_9RHIZ Urea ABC transporter ATP-binding protein UrtD OS=Phyllobacterium endophyticum OX=1149773 GN=urtD PE=4 SV=1
MM1 pKa = 7.49 IGSIYY6 pKa = 10.6 GDD8 pKa = 3.15 NLYY11 pKa = 11.37 GNNQANTLIGGRR23 pKa = 11.84 GNDD26 pKa = 4.47 DD27 pKa = 3.37 FWAGDD32 pKa = 3.58 GRR34 pKa = 11.84 DD35 pKa = 3.84 TMDD38 pKa = 4.31 GGAGDD43 pKa = 4.61 DD44 pKa = 3.65 QFHH47 pKa = 6.19 YY48 pKa = 10.97 ASLAALDD55 pKa = 3.81 GDD57 pKa = 4.49 RR58 pKa = 11.84 INGFTYY64 pKa = 9.64 GYY66 pKa = 9.07 EE67 pKa = 3.78 QDD69 pKa = 3.87 RR70 pKa = 11.84 VVLTGLGVKK79 pKa = 9.53 FVNIIVDD86 pKa = 3.74 EE87 pKa = 4.7 VIGGVLYY94 pKa = 10.36 YY95 pKa = 10.44 KK96 pKa = 9.48 ITGVGPDD103 pKa = 3.35 YY104 pKa = 10.06 TAEE107 pKa = 3.83 YY108 pKa = 9.39 TIAIDD113 pKa = 3.97 GKK115 pKa = 9.87 GSLFPRR121 pKa = 11.84 EE122 pKa = 3.8 EE123 pKa = 4.35 DD124 pKa = 3.84 VFTLL128 pKa = 4.33
Molecular weight: 13.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.923
IPC_protein 3.91
Toseland 3.681
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.884
Rodwell 3.745
Grimsley 3.592
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.317
Thurlkill 3.757
EMBOSS 3.897
Sillero 4.037
Patrickios 1.939
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.918
Protein with the highest isoelectric point:
>tr|A0A2P7B0F2|A0A2P7B0F2_9RHIZ Serine/threonine protein phosphatase OS=Phyllobacterium endophyticum OX=1149773 GN=CU100_04235 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.43 LVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.38 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATAGGRR28 pKa = 11.84 KK29 pKa = 9.15 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5125
0
5125
1598008
26
3799
311.8
33.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.236 ± 0.04
0.782 ± 0.011
5.695 ± 0.029
5.649 ± 0.034
3.947 ± 0.024
8.327 ± 0.051
2.024 ± 0.018
5.97 ± 0.027
3.905 ± 0.032
9.87 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.508 ± 0.018
3.142 ± 0.023
4.865 ± 0.028
3.179 ± 0.02
6.459 ± 0.034
5.988 ± 0.027
5.454 ± 0.031
7.31 ± 0.029
1.287 ± 0.014
2.403 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here