Bondarzewia mesenterica

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Russulales; Bondarzewiaceae; Bondarzewia

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10514 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S4LSZ6|A0A4S4LSZ6_9AGAM Uncharacterized protein OS=Bondarzewia mesenterica OX=1095465 GN=EW146_g6886 PE=4 SV=1
MM1 pKa = 7.63SSEE4 pKa = 4.22PPSLVSYY11 pKa = 10.61ARR13 pKa = 11.84SKK15 pKa = 10.86LHH17 pKa = 6.3LAVGSGSKK25 pKa = 10.27DD26 pKa = 2.91KK27 pKa = 11.11CSLHH31 pKa = 6.45RR32 pKa = 11.84WVLLKK37 pKa = 11.06NSITRR42 pKa = 11.84SQPSIVPSCNSNSMADD58 pKa = 3.44VNLVYY63 pKa = 10.07NTDD66 pKa = 3.24VGEE69 pKa = 4.36VEE71 pKa = 5.08VEE73 pKa = 4.18DD74 pKa = 4.56EE75 pKa = 4.45DD76 pKa = 4.12VCSEE80 pKa = 4.2EE81 pKa = 4.14EE82 pKa = 3.87HH83 pKa = 7.16DD84 pKa = 5.33SFMFPDD90 pKa = 4.18AGRR93 pKa = 11.84LVHH96 pKa = 6.61QSRR99 pKa = 11.84SSDD102 pKa = 3.18ADD104 pKa = 3.23GSEE107 pKa = 4.17SQWLDD112 pKa = 3.27SLLEE116 pKa = 4.05TLGEE120 pKa = 4.07EE121 pKa = 5.67DD122 pKa = 3.6EE123 pKa = 5.52DD124 pKa = 4.82EE125 pKa = 4.64YY126 pKa = 11.76DD127 pKa = 4.09SDD129 pKa = 3.99VHH131 pKa = 6.27PSLMHH136 pKa = 7.15GDD138 pKa = 4.82DD139 pKa = 3.64EE140 pKa = 5.3DD141 pKa = 4.27EE142 pKa = 4.77PPFTPSTSPMSSSDD156 pKa = 3.95DD157 pKa = 3.71LTIHH161 pKa = 6.65PVYY164 pKa = 10.18FDD166 pKa = 4.42PPISMPYY173 pKa = 9.13PIVYY177 pKa = 9.33PPYY180 pKa = 9.09HH181 pKa = 6.25SQFIPALEE189 pKa = 4.13FGPLNSPLDD198 pKa = 3.66SSYY201 pKa = 11.38PPLNVALPYY210 pKa = 10.65YY211 pKa = 10.63DD212 pKa = 5.46ADD214 pKa = 4.55DD215 pKa = 4.62VDD217 pKa = 5.21DD218 pKa = 5.82LPVPDD223 pKa = 5.7AIEE226 pKa = 4.97DD227 pKa = 3.87EE228 pKa = 4.73SDD230 pKa = 3.89DD231 pKa = 4.23EE232 pKa = 5.55SDD234 pKa = 4.57ALSTPSNSRR243 pKa = 11.84SISSFVEE250 pKa = 3.81PSTPQSRR257 pKa = 11.84QRR259 pKa = 11.84PLPQVYY265 pKa = 9.86VDD267 pKa = 4.26SDD269 pKa = 3.46DD270 pKa = 4.44SYY272 pKa = 11.75FYY274 pKa = 10.55PFEE277 pKa = 5.38LDD279 pKa = 3.73PLPFPDD285 pKa = 4.66DD286 pKa = 3.69HH287 pKa = 6.24RR288 pKa = 11.84TSPLVFNNAYY298 pKa = 9.83HH299 pKa = 5.4QEE301 pKa = 4.23CC302 pKa = 4.16

Molecular weight: 33.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S4LU38|A0A4S4LU38_9AGAM Serine/threonine-protein kinase Tel1 OS=Bondarzewia mesenterica OX=1095465 GN=EW146_g5182 PE=3 SV=1
MM1 pKa = 7.04TGARR5 pKa = 11.84RR6 pKa = 11.84TGRR9 pKa = 11.84VVSRR13 pKa = 11.84VHH15 pKa = 6.17EE16 pKa = 4.27LGGNGNGNGSAQRR29 pKa = 11.84IFLFTSRR36 pKa = 11.84PAAPPQLHH44 pKa = 6.61PRR46 pKa = 11.84LRR48 pKa = 11.84PPSHH52 pKa = 6.81NKK54 pKa = 9.28PPPPPPHH61 pKa = 5.93TTTPAAPAAVANPRR75 pKa = 11.84PPARR79 pKa = 11.84TPHH82 pKa = 5.7TPAA85 pKa = 5.36

Molecular weight: 8.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10514

0

10514

5388005

35

5577

512.5

56.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.622 ± 0.018

1.209 ± 0.008

5.636 ± 0.014

5.86 ± 0.022

3.728 ± 0.015

6.284 ± 0.017

2.68 ± 0.012

4.835 ± 0.014

4.35 ± 0.019

9.222 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.106 ± 0.008

3.259 ± 0.011

6.617 ± 0.026

3.634 ± 0.013

6.358 ± 0.018

9.038 ± 0.029

5.921 ± 0.014

6.347 ± 0.015

1.387 ± 0.007

2.543 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski