Trailing lespedeza virus 1
Average proteome isoelectric point is 7.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F1BA31|F1BA31_9TOMB Putative movement protein 1 OS=Trailing lespedeza virus 1 OX=944580 PE=4 SV=1
MM1 pKa = 7.4 FRR3 pKa = 11.84 DD4 pKa = 3.95 LCRR7 pKa = 11.84 YY8 pKa = 9.27 ASQIVVGSTVLAVNVSVLSVAVQLRR33 pKa = 11.84 GAVAVYY39 pKa = 9.09 EE40 pKa = 4.35 VASKK44 pKa = 10.84 CVTALEE50 pKa = 4.72 DD51 pKa = 3.86 CVSDD55 pKa = 5.13 LRR57 pKa = 11.84 DD58 pKa = 3.47 SLKK61 pKa = 10.51 PVPDD65 pKa = 3.51 EE66 pKa = 4.71 LYY68 pKa = 11.09 NPSLCAALRR77 pKa = 11.84 MNNTDD82 pKa = 3.52 TEE84 pKa = 4.41 LTAEE88 pKa = 4.71 DD89 pKa = 5.31 LLTTDD94 pKa = 3.62 RR95 pKa = 11.84 VLVNTWEE102 pKa = 3.92 TDD104 pKa = 3.21 EE105 pKa = 5.84 AKK107 pKa = 10.43 FKK109 pKa = 10.78 YY110 pKa = 9.63 RR111 pKa = 11.84 YY112 pKa = 8.79 KK113 pKa = 11.02 ARR115 pKa = 11.84 IQSGQRR121 pKa = 11.84 GRR123 pKa = 11.84 FMYY126 pKa = 10.48 EE127 pKa = 3.51 LVAYY131 pKa = 8.46 GKK133 pKa = 9.46 NHH135 pKa = 6.76 FGRR138 pKa = 11.84 APRR141 pKa = 11.84 CCDD144 pKa = 3.2 SNYY147 pKa = 10.52 LSVTRR152 pKa = 11.84 LLVNRR157 pKa = 11.84 CNEE160 pKa = 3.81 LHH162 pKa = 6.16 LTPTQTRR169 pKa = 11.84 NVVAEE174 pKa = 3.99 AVSLVFIPDD183 pKa = 3.41 EE184 pKa = 4.15 EE185 pKa = 4.78 EE186 pKa = 3.64 IRR188 pKa = 11.84 QTMLTNSHH196 pKa = 5.7 YY197 pKa = 11.04 AYY199 pKa = 9.23 MQRR202 pKa = 11.84 TRR204 pKa = 11.84 QSVVSTVEE212 pKa = 3.96 PWWKK216 pKa = 10.2 EE217 pKa = 3.7 LLLGPSNAKK226 pKa = 8.27 NWRR229 pKa = 11.84 RR230 pKa = 11.84 AWIEE234 pKa = 3.63 ICGLPEE240 pKa = 3.64 KK241 pKa = 10.39 QAISFAKK248 pKa = 10.36
Molecular weight: 28.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.916
IPC2_protein 7.117
IPC_protein 7.088
Toseland 6.649
ProMoST 7.775
Dawson 7.746
Bjellqvist 8.083
Wikipedia 7.658
Rodwell 7.746
Grimsley 6.664
Solomon 7.819
Lehninger 7.834
Nozaki 8.346
DTASelect 7.878
Thurlkill 7.922
EMBOSS 7.966
Sillero 8.273
Patrickios 4.113
IPC_peptide 7.805
IPC2_peptide 7.483
IPC2.peptide.svr19 7.392
Protein with the highest isoelectric point:
>tr|F1BA33|F1BA33_9TOMB Capsid protein OS=Trailing lespedeza virus 1 OX=944580 PE=3 SV=1
MM1 pKa = 6.87 SHH3 pKa = 6.38 SRR5 pKa = 11.84 GLIHH9 pKa = 6.82 TLLVLLLVVSLLWLKK24 pKa = 9.69 QYY26 pKa = 10.22 RR27 pKa = 11.84 WSITLTFDD35 pKa = 2.98 SSLNRR40 pKa = 11.84 VIALALLGAVLTSVGNSSTVYY61 pKa = 10.55 YY62 pKa = 10.28 INNSTNEE69 pKa = 3.8 NKK71 pKa = 9.62 TNHH74 pKa = 5.86 IKK76 pKa = 10.34 IEE78 pKa = 4.17 TT79 pKa = 3.68
Molecular weight: 8.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.148
IPC2_protein 9.326
IPC_protein 9.472
Toseland 9.692
ProMoST 9.516
Dawson 9.984
Bjellqvist 9.692
Wikipedia 10.204
Rodwell 10.204
Grimsley 10.087
Solomon 10.058
Lehninger 10.028
Nozaki 9.633
DTASelect 9.706
Thurlkill 9.794
EMBOSS 10.101
Sillero 9.882
Patrickios 7.585
IPC_peptide 10.058
IPC2_peptide 8.375
IPC2.peptide.svr19 8.249
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1510
67
770
302.0
33.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.079 ± 0.766
2.583 ± 0.548
4.437 ± 0.341
5.099 ± 0.899
3.444 ± 0.492
5.497 ± 0.721
2.185 ± 0.76
4.636 ± 0.657
4.437 ± 0.206
8.874 ± 1.376
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.921 ± 0.508
5.364 ± 0.387
3.841 ± 0.471
3.311 ± 0.295
6.954 ± 0.717
7.483 ± 1.42
7.02 ± 1.216
9.073 ± 0.822
1.854 ± 0.141
3.841 ± 0.472
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here