Lysinimonas sp. KACC 19322
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2341 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C1Y5L6|A0A5C1Y5L6_9MICO Uncharacterized protein OS=Lysinimonas sp. KACC 19322 OX=2592654 GN=FLP23_03645 PE=4 SV=1
MM1 pKa = 7.28 SASPTSRR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 SYY12 pKa = 11.5 AGIAVAAFLATALTACFPGGGDD34 pKa = 3.42 SKK36 pKa = 11.52 SGGGVSYY43 pKa = 10.92 DD44 pKa = 4.0 GEE46 pKa = 4.69 DD47 pKa = 3.37 SAAVQSATIQFSGQGTFVEE66 pKa = 5.19 PDD68 pKa = 3.4 TIDD71 pKa = 3.49 PAEE74 pKa = 4.04 YY75 pKa = 8.97 RR76 pKa = 11.84 WSGSGFFITSDD87 pKa = 3.99 GIAVTNNHH95 pKa = 5.51 VVTGAGSLSVTIGGEE110 pKa = 3.98 GDD112 pKa = 3.72 SYY114 pKa = 10.64 PVRR117 pKa = 11.84 VLSTSEE123 pKa = 4.02 CLDD126 pKa = 3.33 IAVVQVDD133 pKa = 3.17 VDD135 pKa = 3.79 KK136 pKa = 10.81 PVPYY140 pKa = 10.35 FDD142 pKa = 3.34 WAEE145 pKa = 4.11 GKK147 pKa = 10.13 ISTGSDD153 pKa = 3.49 VYY155 pKa = 11.69 ALGYY159 pKa = 9.59 PLGDD163 pKa = 3.57 PTFSMTKK170 pKa = 9.21 GAVVKK175 pKa = 10.44 SDD177 pKa = 3.4 YY178 pKa = 11.68 AFDD181 pKa = 3.65 TFDD184 pKa = 3.32 NVVAHH189 pKa = 7.2 GIQSDD194 pKa = 3.52 AAIRR198 pKa = 11.84 GGNSGGPLINEE209 pKa = 4.06 KK210 pKa = 10.8 GKK212 pKa = 10.03 VVGVNFYY219 pKa = 11.58 GNDD222 pKa = 3.23 NLATSNYY229 pKa = 10.33 AIGRR233 pKa = 11.84 DD234 pKa = 3.59 DD235 pKa = 4.19 VLPVLDD241 pKa = 4.84 DD242 pKa = 4.34 LKK244 pKa = 11.05 AGKK247 pKa = 9.82 SVLSLGLNLSALTTDD262 pKa = 4.09 DD263 pKa = 4.47 PYY265 pKa = 11.47 GLSGIWVQSVAEE277 pKa = 4.56 GSAADD282 pKa = 3.77 KK283 pKa = 11.24 AGIEE287 pKa = 4.37 PGDD290 pKa = 3.76 VLQKK294 pKa = 10.99 LGGVTLAGASSLSQFEE310 pKa = 4.35 NTTEE314 pKa = 3.99 GTMKK318 pKa = 10.54 NYY320 pKa = 10.39 CQVLQTKK327 pKa = 10.24 GVDD330 pKa = 3.35 APMGAVVYY338 pKa = 10.49 RR339 pKa = 11.84 PATDD343 pKa = 3.27 EE344 pKa = 4.11 LLEE347 pKa = 4.16 GEE349 pKa = 4.86 INGSDD354 pKa = 4.22 PIEE357 pKa = 4.15 VTASGVLGGGGDD369 pKa = 3.8 GGNVSSGYY377 pKa = 9.14 TDD379 pKa = 3.08 IVSDD383 pKa = 4.27 DD384 pKa = 3.32 YY385 pKa = 11.37 RR386 pKa = 11.84 IAVTVPAEE394 pKa = 4.06 WNQVATTPGAGEE406 pKa = 4.21 TLLQASSDD414 pKa = 3.62 LGSFGSLAAPGVEE427 pKa = 4.18 LHH429 pKa = 5.95 STDD432 pKa = 3.8 YY433 pKa = 10.8 QALGAPADD441 pKa = 4.05 VIATLDD447 pKa = 3.85 LGLTNLGCTPSSGQIDD463 pKa = 3.61 QDD465 pKa = 4.13 YY466 pKa = 11.69 ADD468 pKa = 4.1 GQYY471 pKa = 10.97 SGLFSVYY478 pKa = 9.36 TSCPTGADD486 pKa = 2.95 AVVVVVDD493 pKa = 4.87 ADD495 pKa = 3.51 NGDD498 pKa = 3.24 ASGYY502 pKa = 10.77 LLILGTFGDD511 pKa = 4.58 YY512 pKa = 10.82 ASSDD516 pKa = 3.54 PGVLKK521 pKa = 10.81 VLNTFTLSS529 pKa = 3.14
Molecular weight: 53.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.795
IPC_protein 3.846
Toseland 3.605
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.795
Rodwell 3.656
Grimsley 3.516
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.24
Thurlkill 3.668
EMBOSS 3.795
Sillero 3.961
Patrickios 1.113
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.843
Protein with the highest isoelectric point:
>tr|A0A5C1YCP6|A0A5C1YCP6_9MICO ABC transporter ATP-binding protein OS=Lysinimonas sp. KACC 19322 OX=2592654 GN=FLP23_10960 PE=4 SV=1
MM1 pKa = 7.44 TIKK4 pKa = 10.42 PSRR7 pKa = 11.84 VSVRR11 pKa = 11.84 PRR13 pKa = 11.84 PTGRR17 pKa = 11.84 PIRR20 pKa = 11.84 GHH22 pKa = 5.36 RR23 pKa = 11.84 PRR25 pKa = 11.84 RR26 pKa = 11.84 PRR28 pKa = 11.84 GPGRR32 pKa = 11.84 RR33 pKa = 11.84 GRR35 pKa = 11.84 ARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 GGRR43 pKa = 11.84 GTRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 GRR50 pKa = 11.84 GQRR53 pKa = 11.84 WGSWVVQTKK62 pKa = 10.09 RR63 pKa = 11.84 EE64 pKa = 4.09 PGRR67 pKa = 11.84 DD68 pKa = 3.02 AGATSRR74 pKa = 11.84 EE75 pKa = 4.21 ATGLGLWARR84 pKa = 11.84 RR85 pKa = 11.84 RR86 pKa = 11.84 GVAAAWRR93 pKa = 11.84 GRR95 pKa = 11.84 PGRR98 pKa = 11.84 TRR100 pKa = 11.84 AGLRR104 pKa = 11.84 RR105 pKa = 11.84 RR106 pKa = 11.84 HH107 pKa = 5.92 RR108 pKa = 11.84 SS109 pKa = 2.98
Molecular weight: 12.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.502
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.427
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.135
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.178
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2341
0
2341
777487
26
9623
332.1
35.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.014 ± 0.1
0.437 ± 0.012
5.917 ± 0.042
5.79 ± 0.068
3.13 ± 0.035
9.16 ± 0.076
1.895 ± 0.026
4.521 ± 0.038
1.874 ± 0.037
10.471 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.438 ± 0.023
1.841 ± 0.025
5.591 ± 0.053
2.638 ± 0.03
7.539 ± 0.086
5.355 ± 0.055
5.806 ± 0.082
9.101 ± 0.053
1.504 ± 0.023
1.975 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here