Altererythrobacter soli
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2807 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I4UX55|A0A6I4UX55_9SPHN DUF1178 family protein OS=Altererythrobacter soli OX=1739690 GN=GRI75_11885 PE=4 SV=1
MM1 pKa = 7.3 SLSAKK6 pKa = 10.07 SRR8 pKa = 11.84 LLATSAVAGLCVWQTHH24 pKa = 5.71 LPAAAQDD31 pKa = 3.45 AFGIHH36 pKa = 6.59 VDD38 pKa = 3.62 TPEE41 pKa = 4.02 GQVIVIEE48 pKa = 4.16 NGEE51 pKa = 4.08 IVEE54 pKa = 4.08 GDD56 pKa = 3.64 RR57 pKa = 11.84 IGVYY61 pKa = 10.35 AEE63 pKa = 4.0 QGALDD68 pKa = 3.86 LTVEE72 pKa = 4.17 TGARR76 pKa = 11.84 VRR78 pKa = 11.84 GNGFYY83 pKa = 10.61 DD84 pKa = 3.94 GFGAPPEE91 pKa = 4.33 AGVTIATAGSSVDD104 pKa = 3.4 NSGSISGAGAGITTAYY120 pKa = 10.35 VYY122 pKa = 11.05 DD123 pKa = 3.98 SAADD127 pKa = 3.67 MLVGQAIGTVVVNNGTIAGDD147 pKa = 3.67 SNDD150 pKa = 3.22 GVRR153 pKa = 11.84 LIGGGSVTNSGTILGANWDD172 pKa = 3.97 FADD175 pKa = 5.4 GISMYY180 pKa = 10.76 AYY182 pKa = 10.2 ADD184 pKa = 3.57 QAKK187 pKa = 9.3 EE188 pKa = 4.1 DD189 pKa = 3.92 YY190 pKa = 10.33 SALVTNEE197 pKa = 3.71 AGGEE201 pKa = 3.87 IAGQRR206 pKa = 11.84 FGVILSGGGDD216 pKa = 3.57 VVNDD220 pKa = 3.63 GDD222 pKa = 3.83 ITGVAGGVLVQGTALDD238 pKa = 3.74 SEE240 pKa = 4.57 EE241 pKa = 4.85 RR242 pKa = 11.84 SGLTASVVNSGTISGTGSFGGTYY265 pKa = 9.87 TGGQGVAFGSDD276 pKa = 2.83 MAAATLVNSGTITSAFAEE294 pKa = 4.36 AVSQGSLADD303 pKa = 3.54 LTITNEE309 pKa = 3.66 QSGVIEE315 pKa = 4.9 GGTSGIYY322 pKa = 10.19 GGSSGTMSIINAGTIRR338 pKa = 11.84 GNGSYY343 pKa = 10.5 DD344 pKa = 3.46 GFGAPPDD351 pKa = 3.76 AGITIATAGSSVDD364 pKa = 3.36 NSGTISGAGAGITTAYY380 pKa = 10.33 VYY382 pKa = 11.04 DD383 pKa = 4.04 SATDD387 pKa = 3.6 TLVGQAIGTVVVNSGTIAGDD407 pKa = 3.51 SNDD410 pKa = 3.22 GVRR413 pKa = 11.84 LIGGGSVTNSGTILGANWDD432 pKa = 3.97 FADD435 pKa = 5.4 GISMYY440 pKa = 10.76 AYY442 pKa = 10.2 ADD444 pKa = 3.57 QAKK447 pKa = 9.3 EE448 pKa = 4.1 DD449 pKa = 3.92 YY450 pKa = 10.33 SALVTNEE457 pKa = 3.71 AGGEE461 pKa = 3.87 IAGQRR466 pKa = 11.84 FGVILSGGGDD476 pKa = 3.57 VVNDD480 pKa = 3.63 GDD482 pKa = 3.83 ITGVAGGVLVQGTALDD498 pKa = 3.74 SEE500 pKa = 4.57 EE501 pKa = 4.85 RR502 pKa = 11.84 SGLTASVVNSGTISGTGSLASGVAFGSDD530 pKa = 2.86 MAAATLVNSGTINGAAAGIEE550 pKa = 4.51 AYY552 pKa = 10.53 FSGAFSLANTGTVTGEE568 pKa = 4.23 SIGVDD573 pKa = 3.31 SGALGAVTLNNSGSITGEE591 pKa = 3.69 SGPAVVSASQTTLINSGSLHH611 pKa = 5.96 GAGGVAVQLAEE622 pKa = 4.18 YY623 pKa = 9.74 DD624 pKa = 3.64 DD625 pKa = 5.01 SVTLRR630 pKa = 11.84 TGSSVVGTIDD640 pKa = 4.36 AGDD643 pKa = 3.8 GTDD646 pKa = 3.5 ILILEE651 pKa = 4.54 GDD653 pKa = 4.08 VLGLTSTQVIGSAEE667 pKa = 3.91 NFEE670 pKa = 4.5 TLRR673 pKa = 11.84 VATGYY678 pKa = 7.1 WTTASYY684 pKa = 11.24 VGDD687 pKa = 4.18 FEE689 pKa = 4.72 QVAILEE695 pKa = 4.57 GASLQVNEE703 pKa = 4.47 VTVDD707 pKa = 3.52 AQVTSPILTSDD718 pKa = 3.69 VLNDD722 pKa = 3.49 GTLVLNFDD730 pKa = 3.59 HH731 pKa = 8.52 VEE733 pKa = 3.79 IDD735 pKa = 3.38 EE736 pKa = 4.35 LAISGSGGVTLIGEE750 pKa = 4.51 AVYY753 pKa = 10.31 EE754 pKa = 4.19 VTGNSLTYY762 pKa = 10.48 TGDD765 pKa = 3.33 TVIANGGVILSGSLDD780 pKa = 3.65 SDD782 pKa = 3.94 VVTQGDD788 pKa = 4.12 GFFTLGLGGTAGTYY802 pKa = 9.9 VGSIVNNGVFNFDD815 pKa = 3.33 RR816 pKa = 11.84 SDD818 pKa = 3.3 NYY820 pKa = 10.93 EE821 pKa = 3.83 FLGDD825 pKa = 3.79 FSGSGTLNKK834 pKa = 10.09 FGAGTLTFSGLYY846 pKa = 10.43 DD847 pKa = 3.87 FGGTTTINAGAIAFAGQLAEE867 pKa = 4.43 DD868 pKa = 4.1 TQLDD872 pKa = 3.97 LSEE875 pKa = 5.45 GGTFDD880 pKa = 4.94 LSQIEE885 pKa = 4.51 SGEE888 pKa = 3.83 QTIAEE893 pKa = 4.08 LSGTGGTLLLGDD905 pKa = 4.05 TDD907 pKa = 3.96 LTVQQTADD915 pKa = 3.53 SVFAGAVTGTGTLLKK930 pKa = 10.74 DD931 pKa = 3.52 GSGDD935 pKa = 3.54 LKK937 pKa = 11.42 LNGDD941 pKa = 3.71 GTGFTGTGQVDD952 pKa = 3.8 GGTLSVNGDD961 pKa = 3.52 FSNANFLVNDD971 pKa = 4.32 GGTLGGAGTLGSTTVSGGTLAPGNSIDD998 pKa = 3.88 TITFVGDD1005 pKa = 3.56 LTLTAASVYY1014 pKa = 9.51 EE1015 pKa = 4.28 VEE1017 pKa = 4.67 VDD1019 pKa = 3.36 PAGNSDD1025 pKa = 3.55 RR1026 pKa = 11.84 TNVTGTATLGNATVNVLAEE1045 pKa = 4.18 SGLYY1049 pKa = 10.48 GPATDD1054 pKa = 3.97 YY1055 pKa = 11.14 TILTAEE1061 pKa = 4.51 NGISGQFGEE1070 pKa = 4.59 VQTNLAYY1077 pKa = 10.44 LVPLLSYY1084 pKa = 10.23 TVDD1087 pKa = 3.41 TVSLRR1092 pKa = 11.84 LSRR1095 pKa = 11.84 NDD1097 pKa = 3.13 IDD1099 pKa = 3.93 FSTYY1103 pKa = 10.96 AATPNQATVGALIEE1117 pKa = 4.24 GLEE1120 pKa = 4.41 FGTPLYY1126 pKa = 10.92 DD1127 pKa = 3.26 ATLVLARR1134 pKa = 11.84 DD1135 pKa = 3.56 QVATNFEE1142 pKa = 4.46 TLTGGVYY1149 pKa = 7.91 PTYY1152 pKa = 10.41 GASLVEE1158 pKa = 3.83 TAYY1161 pKa = 10.16 MLRR1164 pKa = 11.84 RR1165 pKa = 11.84 QAAPQPQAAVGAFVWGTGLAGEE1187 pKa = 4.99 LSASGSDD1194 pKa = 3.1 GVTGFKK1200 pKa = 10.0 TDD1202 pKa = 3.07 NAGAAGGLGYY1212 pKa = 10.67 AADD1215 pKa = 4.17 GFDD1218 pKa = 3.56 FTVGLGKK1225 pKa = 10.48 LWQDD1229 pKa = 3.37 PDD1231 pKa = 3.4 RR1232 pKa = 11.84 SQISDD1237 pKa = 3.57 SDD1239 pKa = 3.92 VKK1241 pKa = 10.79 FALAQASYY1249 pKa = 10.45 ASTMGLSARR1258 pKa = 11.84 AGVQFGWIDD1267 pKa = 3.41 GAASRR1272 pKa = 11.84 NTRR1275 pKa = 11.84 LGTISATVSSAFEE1288 pKa = 4.04 GEE1290 pKa = 4.03 YY1291 pKa = 9.42 TSYY1294 pKa = 10.94 YY1295 pKa = 10.77 GEE1297 pKa = 4.28 IGYY1300 pKa = 9.78 ALPMGVASIEE1310 pKa = 3.99 PFAGLNHH1317 pKa = 6.12 VSLDD1321 pKa = 3.21 MDD1323 pKa = 3.98 RR1324 pKa = 11.84 FTEE1327 pKa = 4.23 VGGATALTVDD1337 pKa = 3.65 GLDD1340 pKa = 3.51 RR1341 pKa = 11.84 DD1342 pKa = 3.97 VTFADD1347 pKa = 3.52 LGLRR1351 pKa = 11.84 LAANPVGGVAPYY1363 pKa = 10.72 LSGAYY1368 pKa = 8.89 RR1369 pKa = 11.84 RR1370 pKa = 11.84 AWGDD1374 pKa = 3.16 RR1375 pKa = 11.84 MSEE1378 pKa = 3.72 ATIAFTGNSAGGTITGLPIARR1399 pKa = 11.84 SAAEE1403 pKa = 3.89 LEE1405 pKa = 3.98 AGVRR1409 pKa = 11.84 YY1410 pKa = 10.27 SGGVVDD1416 pKa = 6.24 LEE1418 pKa = 4.3 LGYY1421 pKa = 9.35 TGSFSKK1427 pKa = 9.73 TFDD1430 pKa = 3.61 SNSVKK1435 pKa = 10.36 VSASIHH1441 pKa = 5.03 FF1442 pKa = 4.14
Molecular weight: 143.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.656
IPC_protein 3.706
Toseland 3.478
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.528
Grimsley 3.376
Solomon 3.694
Lehninger 3.656
Nozaki 3.795
DTASelect 4.062
Thurlkill 3.516
EMBOSS 3.643
Sillero 3.821
Patrickios 1.558
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A6I4UPF9|A0A6I4UPF9_9SPHN Uncharacterized protein OS=Altererythrobacter soli OX=1739690 GN=GRI75_04005 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 SRR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.5 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 KK22 pKa = 7.81 ATVGGRR28 pKa = 11.84 KK29 pKa = 8.12 VLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.02 KK41 pKa = 10.61 LSAA44 pKa = 4.03
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2807
0
2807
910999
38
4886
324.5
35.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.047 ± 0.071
0.816 ± 0.017
5.795 ± 0.039
6.414 ± 0.062
3.564 ± 0.029
9.118 ± 0.081
1.932 ± 0.026
4.662 ± 0.025
2.798 ± 0.046
9.938 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.305 ± 0.026
2.471 ± 0.039
5.443 ± 0.041
3.221 ± 0.025
7.438 ± 0.066
5.21 ± 0.043
5.092 ± 0.076
7.147 ± 0.038
1.412 ± 0.02
2.177 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here