Prevotella bergensis DSM 17361
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2824 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D1PXM4|D1PXM4_9BACT Polysaccharide biosynthesis protein OS=Prevotella bergensis DSM 17361 OX=585502 GN=HMPREF0645_1709 PE=4 SV=1
MM1 pKa = 7.69 RR2 pKa = 11.84 MKK4 pKa = 10.54 KK5 pKa = 9.83 ISTNVFVILLAVAPILFASCGSDD28 pKa = 3.88 PVYY31 pKa = 10.61 TDD33 pKa = 2.85 PWYY36 pKa = 10.19 DD37 pKa = 3.04 DD38 pKa = 3.6 WTWGDD43 pKa = 4.81 DD44 pKa = 3.65 YY45 pKa = 11.49 SQRR48 pKa = 11.84 PSDD51 pKa = 4.63 DD52 pKa = 3.54 NVSDD56 pKa = 3.74 EE57 pKa = 4.61 DD58 pKa = 4.26 FLVSMAQTLAGQWRR72 pKa = 11.84 GDD74 pKa = 3.39 MMAYY78 pKa = 10.1 EE79 pKa = 4.84 IDD81 pKa = 3.66 NAGNAIDD88 pKa = 4.09 SIYY91 pKa = 10.63 YY92 pKa = 8.5 KK93 pKa = 10.41 TDD95 pKa = 3.09 IEE97 pKa = 4.41 FKK99 pKa = 10.32 QYY101 pKa = 10.31 NRR103 pKa = 11.84 QSISGTGTQYY113 pKa = 11.39 DD114 pKa = 3.76 FHH116 pKa = 7.61 PDD118 pKa = 3.37 TEE120 pKa = 4.25 EE121 pKa = 5.98 LEE123 pKa = 4.28 LQRR126 pKa = 11.84 DD127 pKa = 4.21 FTWYY131 pKa = 10.56 VDD133 pKa = 3.67 PKK135 pKa = 11.03 SGDD138 pKa = 3.17 VYY140 pKa = 9.26 MTT142 pKa = 4.05
Molecular weight: 16.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.884
IPC_protein 3.884
Toseland 3.656
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.859
Rodwell 3.706
Grimsley 3.567
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.304
Thurlkill 3.719
EMBOSS 3.872
Sillero 4.012
Patrickios 1.926
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.903
Protein with the highest isoelectric point:
>tr|D1PTC4|D1PTC4_9BACT Transporter gate domain protein OS=Prevotella bergensis DSM 17361 OX=585502 GN=HMPREF0645_0209 PE=4 SV=1
MM1 pKa = 7.6 NIFDD5 pKa = 4.86 HH6 pKa = 6.14 VCKK9 pKa = 10.67 NCIYY13 pKa = 11.19 ALITHH18 pKa = 6.65 EE19 pKa = 4.26 SHH21 pKa = 7.29 RR22 pKa = 11.84 LLHH25 pKa = 6.3 LRR27 pKa = 11.84 QKK29 pKa = 8.69 TSCGLTVGAVGRR41 pKa = 11.84 PCPRR45 pKa = 11.84 PRR47 pKa = 11.84 SPRR50 pKa = 11.84 RR51 pKa = 11.84 VMPDD55 pKa = 3.02 QIATGRR61 pKa = 11.84 KK62 pKa = 9.07 SYY64 pKa = 10.83 NCPFRR69 pKa = 11.84 FRR71 pKa = 11.84 EE72 pKa = 4.68 FKK74 pKa = 10.57 NQPPPSLKK82 pKa = 9.53 FAKK85 pKa = 9.52 FVKK88 pKa = 10.27 FVVPP92 pKa = 4.5
Molecular weight: 10.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.648
IPC_protein 10.189
Toseland 10.745
ProMoST 10.35
Dawson 10.818
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 11.14
Grimsley 10.847
Solomon 10.921
Lehninger 10.891
Nozaki 10.76
DTASelect 10.482
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.76
Patrickios 10.906
IPC_peptide 10.921
IPC2_peptide 9.78
IPC2.peptide.svr19 8.546
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2824
0
2824
942703
38
2547
333.8
37.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.225 ± 0.042
1.225 ± 0.018
5.876 ± 0.032
5.93 ± 0.041
4.391 ± 0.027
6.969 ± 0.042
2.115 ± 0.023
6.388 ± 0.046
6.533 ± 0.039
8.67 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.037 ± 0.022
5.204 ± 0.038
3.672 ± 0.022
3.692 ± 0.028
5.046 ± 0.037
6.013 ± 0.035
5.783 ± 0.032
6.619 ± 0.037
1.302 ± 0.017
4.308 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here