Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Formosa; Formosa agariphila

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3557 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T2KLV0|T2KLV0_FORAG Macrophage infectivity potentiator-related protein OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901) OX=1347342 GN=BN863_16890 PE=4 SV=1
MM1 pKa = 7.08ITNSTKK7 pKa = 10.73VLVLCALALSLFNCSSEE24 pKa = 4.61DD25 pKa = 3.44DD26 pKa = 3.61TDD28 pKa = 3.62RR29 pKa = 11.84GNWQEE34 pKa = 3.87RR35 pKa = 11.84SVFDD39 pKa = 3.48GVPRR43 pKa = 11.84SSVVGFVIDD52 pKa = 3.32NLGYY56 pKa = 10.05MGTGYY61 pKa = 10.9DD62 pKa = 3.68GDD64 pKa = 5.47DD65 pKa = 3.66YY66 pKa = 11.53LTDD69 pKa = 3.65FWEE72 pKa = 4.24YY73 pKa = 10.99NIEE76 pKa = 3.67GDD78 pKa = 3.85YY79 pKa = 10.02WVQKK83 pKa = 11.04ADD85 pKa = 3.7FPGTEE90 pKa = 3.88RR91 pKa = 11.84SAASGFTIGGLGYY104 pKa = 10.67LGLGYY109 pKa = 10.68DD110 pKa = 4.07GTDD113 pKa = 3.2EE114 pKa = 6.11LKK116 pKa = 11.04DD117 pKa = 3.59FWEE120 pKa = 4.45YY121 pKa = 11.66NPGSNAWTQKK131 pKa = 10.84ADD133 pKa = 3.41FLGGVRR139 pKa = 11.84QAAIGFAANNTGYY152 pKa = 10.98VGTGYY157 pKa = 10.89DD158 pKa = 3.69GDD160 pKa = 3.92NDD162 pKa = 3.77RR163 pKa = 11.84KK164 pKa = 10.76DD165 pKa = 3.62FYY167 pKa = 11.03KK168 pKa = 11.01YY169 pKa = 11.09NPTTDD174 pKa = 2.84TWSEE178 pKa = 3.74LVGFGGEE185 pKa = 3.79KK186 pKa = 9.99RR187 pKa = 11.84RR188 pKa = 11.84FATTFNIGDD197 pKa = 3.68KK198 pKa = 10.7VYY200 pKa = 10.65IGTGVSNGLYY210 pKa = 8.68KK211 pKa = 10.35TDD213 pKa = 3.62FWEE216 pKa = 4.39FDD218 pKa = 3.8PLSEE222 pKa = 4.28TFSRR226 pKa = 11.84LNDD229 pKa = 3.66LDD231 pKa = 4.99EE232 pKa = 4.97EE233 pKa = 4.75DD234 pKa = 5.58DD235 pKa = 3.96YY236 pKa = 11.87TITRR240 pKa = 11.84SNAVGFSVDD249 pKa = 2.94GLGYY253 pKa = 10.03IVSGYY258 pKa = 9.69NGSVLGSTWEE268 pKa = 4.07YY269 pKa = 11.67SPGTDD274 pKa = 2.18SWEE277 pKa = 4.24EE278 pKa = 3.54ITGIEE283 pKa = 4.31AYY285 pKa = 10.23SRR287 pKa = 11.84QDD289 pKa = 3.34ALAFSNDD296 pKa = 2.25SRR298 pKa = 11.84AFVLLGRR305 pKa = 11.84SGSLYY310 pKa = 10.73LDD312 pKa = 4.16DD313 pKa = 5.42NYY315 pKa = 11.1EE316 pKa = 4.53LFPQDD321 pKa = 5.52DD322 pKa = 4.38YY323 pKa = 12.22DD324 pKa = 6.49DD325 pKa = 4.25EE326 pKa = 4.75DD327 pKa = 3.58

Molecular weight: 36.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T2KIR4|T2KIR4_FORAG Putative restriction endonuclease OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901) OX=1347342 GN=BN863_10700 PE=4 SV=1
MM1 pKa = 7.3ARR3 pKa = 11.84IAGVDD8 pKa = 3.06IPKK11 pKa = 10.02QKK13 pKa = 10.48RR14 pKa = 11.84GVISLTYY21 pKa = 9.48IYY23 pKa = 10.73GIGTSRR29 pKa = 11.84AKK31 pKa = 10.55EE32 pKa = 3.63ILAEE36 pKa = 4.21AKK38 pKa = 10.13VDD40 pKa = 3.89EE41 pKa = 4.41NTKK44 pKa = 9.59VQDD47 pKa = 3.34WTDD50 pKa = 3.58DD51 pKa = 3.56QIGSIRR57 pKa = 11.84EE58 pKa = 4.03AVSTFTIEE66 pKa = 3.9GEE68 pKa = 4.24LRR70 pKa = 11.84SEE72 pKa = 4.15TQLSIKK78 pKa = 10.3RR79 pKa = 11.84LMDD82 pKa = 3.67IGCYY86 pKa = 9.51RR87 pKa = 11.84GIRR90 pKa = 11.84HH91 pKa = 6.49RR92 pKa = 11.84AGLPLRR98 pKa = 11.84GQRR101 pKa = 11.84TKK103 pKa = 11.3NNSRR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 9.17GRR112 pKa = 11.84RR113 pKa = 11.84KK114 pKa = 7.81TVANKK119 pKa = 10.07KK120 pKa = 9.89KK121 pKa = 9.38ATKK124 pKa = 10.14

Molecular weight: 13.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3557

0

3557

1231760

37

2753

346.3

39.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.39 ± 0.046

0.724 ± 0.012

5.892 ± 0.034

6.453 ± 0.035

5.133 ± 0.031

6.379 ± 0.042

1.909 ± 0.02

7.819 ± 0.039

7.467 ± 0.047

9.212 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.141 ± 0.023

6.237 ± 0.042

3.421 ± 0.023

3.349 ± 0.022

3.244 ± 0.025

6.565 ± 0.034

6.083 ± 0.04

6.287 ± 0.032

1.114 ± 0.015

4.182 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski