Candidatus Enterovibrio luxaltus
Average proteome isoelectric point is 7.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1986 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A291BC35|A0A291BC35_9GAMM Uncharacterized protein OS=Candidatus Enterovibrio luxaltus OX=1927128 GN=BTN50_2107 PE=4 SV=1
MM1 pKa = 7.26 ATDD4 pKa = 4.59 KK5 pKa = 9.33 PTLTLIGDD13 pKa = 3.89 SEE15 pKa = 4.61 VIEE18 pKa = 5.22 GEE20 pKa = 4.05 DD21 pKa = 3.79 ANYY24 pKa = 9.76 TLAISEE30 pKa = 4.66 APISDD35 pKa = 3.56 LTVTVVVGHH44 pKa = 6.03 KK45 pKa = 9.24 TSEE48 pKa = 4.36 DD49 pKa = 3.75 DD50 pKa = 4.05 DD51 pKa = 4.13 VTPITHH57 pKa = 5.61 QVVIAAGTTFTSFTVNTINDD77 pKa = 4.18 GLNEE81 pKa = 4.21 SVDD84 pKa = 3.38 NDD86 pKa = 3.71 VFTLSILGSSGGGFEE101 pKa = 4.76 EE102 pKa = 5.54 LPSTLTAIEE111 pKa = 3.99 TVIRR115 pKa = 11.84 DD116 pKa = 3.93 DD117 pKa = 5.58 AEE119 pKa = 4.46 DD120 pKa = 3.65 AAQLMEE126 pKa = 6.1 DD127 pKa = 4.27 IGTQSISGVLHH138 pKa = 6.01 VLDD141 pKa = 4.81 SNGLTPLEE149 pKa = 4.25 FTNTTVVGNYY159 pKa = 10.17 GSLTLVNGDD168 pKa = 3.28 WTYY171 pKa = 11.98 LLYY174 pKa = 8.92 PTAQILNEE182 pKa = 4.23 GEE184 pKa = 4.14 NVTEE188 pKa = 5.29 FIILTATNGTVQTIVISVIGTEE210 pKa = 4.21 DD211 pKa = 3.52 SAVVTGDD218 pKa = 3.83 FIGSVDD224 pKa = 3.77 VADD227 pKa = 4.94 FSINTEE233 pKa = 3.38 ASNNIRR239 pKa = 11.84 ASNGEE244 pKa = 4.07 IISNANSAWTTTSTMVNNVPYY265 pKa = 10.64 VIATQWGSSGSTIISRR281 pKa = 11.84 VNNDD285 pKa = 2.76 GTLTEE290 pKa = 4.39 TDD292 pKa = 3.86 RR293 pKa = 11.84 ITYY296 pKa = 7.62 NQSSASVITTSGGNITADD314 pKa = 3.31 VRR316 pKa = 11.84 ALGILPEE323 pKa = 4.18 SLGNGLTQSNAANIDD338 pKa = 3.55 GQNTLFVTSQNSSSLTAWNISDD360 pKa = 4.84 SGVLTINGGITNINAEE376 pKa = 4.63 DD377 pKa = 3.8 GQSYY381 pKa = 10.01 IRR383 pKa = 11.84 EE384 pKa = 4.25 NITFEE389 pKa = 3.94 GANGINYY396 pKa = 9.46 IYY398 pKa = 9.29 VTRR401 pKa = 11.84 PGTDD405 pKa = 3.71 GISKK409 pKa = 8.38 FTYY412 pKa = 9.44 SSKK415 pKa = 9.56 TGEE418 pKa = 3.9 MVNTNEE424 pKa = 4.04 FVFGGEE430 pKa = 4.39 TVSSIDD436 pKa = 3.21 VCTTNDD442 pKa = 3.54 YY443 pKa = 11.72 NSFLAAASNDD453 pKa = 3.03 AVRR456 pKa = 11.84 IFAINQSTGALTLVEE471 pKa = 4.3 AVVVPQGTSNSVNYY485 pKa = 9.84 YY486 pKa = 7.92 HH487 pKa = 7.09 TNDD490 pKa = 3.1 NRR492 pKa = 11.84 SYY494 pKa = 10.76 IIYY497 pKa = 10.7 SNNTSNKK504 pKa = 9.63 ASIFQMAEE512 pKa = 3.82 NGSLTLTDD520 pKa = 4.37 TIDD523 pKa = 3.65 GAGHH527 pKa = 5.9 YY528 pKa = 9.13 FSSAGYY534 pKa = 9.39 IDD536 pKa = 4.62 GEE538 pKa = 4.25 PVYY541 pKa = 10.87 IMPNATAGVDD551 pKa = 3.78 LYY553 pKa = 11.03 TIGRR557 pKa = 11.84 NGSFVLQTTVANISNDD573 pKa = 2.74 WTPPVIVQTEE583 pKa = 3.95 DD584 pKa = 2.71 SSYY587 pKa = 11.32 YY588 pKa = 10.79 LVDD591 pKa = 3.85 ADD593 pKa = 4.74 GLASAIKK600 pKa = 9.25 LTVGNTGDD608 pKa = 3.4 GSVGNEE614 pKa = 3.77 GVKK617 pKa = 10.72 VSGSLHH623 pKa = 6.37 ISDD626 pKa = 4.67 IDD628 pKa = 4.01 SEE630 pKa = 4.57 DD631 pKa = 3.49 FSSFEE636 pKa = 4.65 NITITGTYY644 pKa = 7.03 GTLVLVEE651 pKa = 4.17 GTWIYY656 pKa = 10.29 TLDD659 pKa = 3.73 KK660 pKa = 10.83 DD661 pKa = 3.85 KK662 pKa = 11.46 SSNILDD668 pKa = 3.79 DD669 pKa = 5.76 KK670 pKa = 10.28 IAKK673 pKa = 9.73 DD674 pKa = 3.86 AFRR677 pKa = 11.84 LTAIDD682 pKa = 4.32 GTTQDD687 pKa = 4.0 IIIDD691 pKa = 3.93 VTGHH695 pKa = 4.61 EE696 pKa = 4.3 TALEE700 pKa = 4.19 GGVYY704 pKa = 10.38 GKK706 pKa = 8.47 NTDD709 pKa = 3.73 AIIVYY714 pKa = 8.9 LSPQAASPGFIEE726 pKa = 4.94 EE727 pKa = 4.21 SSGDD731 pKa = 3.39 VNFNLRR737 pKa = 11.84 EE738 pKa = 4.0 YY739 pKa = 10.95 VADD742 pKa = 4.14 LVDD745 pKa = 5.68 DD746 pKa = 5.37 ADD748 pKa = 4.67 LSDD751 pKa = 3.64 ARR753 pKa = 11.84 KK754 pKa = 8.04 TSMQIISLPNDD765 pKa = 3.21 GVLYY769 pKa = 10.13 YY770 pKa = 10.93 YY771 pKa = 10.69 NEE773 pKa = 4.61 SGDD776 pKa = 3.77 KK777 pKa = 10.56 VPVVINNVLSDD788 pKa = 3.32 EE789 pKa = 4.64 TIFIFEE795 pKa = 4.81 PNRR798 pKa = 11.84 ASITFSQNDD807 pKa = 3.92 IIIATQTTIISNGITVSGGIFSGTKK832 pKa = 9.58 PDD834 pKa = 3.98 DD835 pKa = 3.76 SSTISPANIVWEE847 pKa = 4.53 FQSGEE852 pKa = 4.06 SGIGVGLDD860 pKa = 3.4 RR861 pKa = 11.84 EE862 pKa = 4.59 ITSSLKK868 pKa = 10.36 EE869 pKa = 3.72 VLTIEE874 pKa = 4.51 FTGSTNVTNADD885 pKa = 3.18 IVVSSAFGNFSDD897 pKa = 4.22 TRR899 pKa = 11.84 SAQGKK904 pKa = 8.06 GQLIAFRR911 pKa = 11.84 DD912 pKa = 4.11 GQVVGEE918 pKa = 3.77 YY919 pKa = 10.33 SYY921 pKa = 12.18 ANLWEE926 pKa = 4.19 ASGGDD931 pKa = 3.72 GVATLNIVNSEE942 pKa = 4.05 GFNEE946 pKa = 3.76 LRR948 pKa = 11.84 LYY950 pKa = 8.87 ITANVNSNFSVTSISASGVNTTDD973 pKa = 2.89 TQFTYY978 pKa = 10.84 KK979 pKa = 10.62 AINSFGNEE987 pKa = 3.9 SEE989 pKa = 4.13 IATVNLGEE997 pKa = 4.47 NIIDD1001 pKa = 4.11 SVTMPAISTVSGTLVIVNSTGVTAVFANTTKK1032 pKa = 10.51 SGTYY1036 pKa = 10.57 GSVTLMNGTWTYY1048 pKa = 9.52 TLHH1051 pKa = 6.92 PALLTVISNNQSIQDD1066 pKa = 4.36 TITLTATDD1074 pKa = 3.79 GTIAEE1079 pKa = 4.2 VTIIISEE1086 pKa = 4.15 SSNAIISYY1094 pKa = 7.62 YY1095 pKa = 7.39 TTIANNSLSGLIDD1108 pKa = 3.2 MGSISFLGNNDD1119 pKa = 3.07 TDD1121 pKa = 4.4 NIYY1124 pKa = 11.03 AGNNNDD1130 pKa = 5.32 GILSLDD1136 pKa = 3.81 GDD1138 pKa = 4.04 DD1139 pKa = 4.02 TVYY1142 pKa = 11.02 SGAGDD1147 pKa = 4.6 DD1148 pKa = 5.49 IIDD1151 pKa = 4.33 LGAGNDD1157 pKa = 3.91 LVVGGAGNDD1166 pKa = 3.32 ILIGGDD1172 pKa = 3.54 GEE1174 pKa = 5.47 DD1175 pKa = 3.84 VFTFLNGNEE1184 pKa = 4.26 GTNEE1188 pKa = 3.96 VPTIDD1193 pKa = 5.28 HH1194 pKa = 6.76 IGDD1197 pKa = 3.79 FNTNQDD1203 pKa = 3.78 ALNVTDD1209 pKa = 5.22 LLQGEE1214 pKa = 4.58 TNDD1217 pKa = 5.28 TIGQYY1222 pKa = 11.27 LNIVMDD1228 pKa = 3.91 SQGYY1232 pKa = 10.46 AMLNISSMGNGMVDD1246 pKa = 3.24 QQVVFDD1252 pKa = 4.27 NLSVNDD1258 pKa = 3.98 MATAYY1263 pKa = 10.58 QIDD1266 pKa = 3.81 ASGISADD1273 pKa = 4.17 QISTLIIDD1281 pKa = 3.84 AMIVQSRR1288 pKa = 11.84 IIIDD1292 pKa = 3.33
Molecular weight: 136.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.605
IPC_protein 3.656
Toseland 3.427
ProMoST 3.808
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.592
Rodwell 3.478
Grimsley 3.325
Solomon 3.656
Lehninger 3.605
Nozaki 3.757
DTASelect 4.024
Thurlkill 3.478
EMBOSS 3.592
Sillero 3.783
Patrickios 1.062
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>tr|A0A291B958|A0A291B958_9GAMM DNA gyrase subunit B OS=Candidatus Enterovibrio luxaltus OX=1927128 GN=gyrB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.12 RR14 pKa = 11.84 SHH16 pKa = 5.87 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MATKK25 pKa = 10.06 HH26 pKa = 4.61 GRR28 pKa = 11.84 KK29 pKa = 9.31 IIAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.91 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSKK44 pKa = 10.84
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.735
Grimsley 13.013
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.457
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1986
0
1986
410369
37
1488
206.6
23.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.689 ± 0.065
1.249 ± 0.024
4.955 ± 0.047
5.651 ± 0.059
4.099 ± 0.042
6.459 ± 0.065
2.417 ± 0.034
7.79 ± 0.052
6.11 ± 0.063
10.215 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.049 ± 0.036
5.018 ± 0.046
3.511 ± 0.034
3.996 ± 0.043
4.901 ± 0.057
6.369 ± 0.054
5.605 ± 0.045
6.855 ± 0.049
1.063 ± 0.023
2.999 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here