Streptococcus phage IPP20

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5S9D8|A0A1S5S9D8_9CAUD Transcriptional regulator Cro/CI family OS=Streptococcus phage IPP20 OX=1916160 GN=IPP20_00006 PE=4 SV=1
MM1 pKa = 7.82TITINFTEE9 pKa = 4.43KK10 pKa = 10.43NSYY13 pKa = 7.68ITDD16 pKa = 3.59YY17 pKa = 9.75LTKK20 pKa = 10.5HH21 pKa = 6.43GIDD24 pKa = 3.51TTTMDD29 pKa = 4.04FDD31 pKa = 4.61DD32 pKa = 5.8FMVLMEE38 pKa = 5.72DD39 pKa = 4.28IEE41 pKa = 4.41DD42 pKa = 4.1ARR44 pKa = 11.84AADD47 pKa = 3.7QAYY50 pKa = 8.84MEE52 pKa = 4.64YY53 pKa = 10.56LADD56 pKa = 4.23PATYY60 pKa = 10.69TMDD63 pKa = 3.45EE64 pKa = 4.26VLDD67 pKa = 3.87EE68 pKa = 5.04LGLSRR73 pKa = 11.84EE74 pKa = 4.63DD75 pKa = 3.39IAA77 pKa = 6.35

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5S9F0|A0A1S5S9F0_9CAUD Uncharacterized protein OS=Streptococcus phage IPP20 OX=1916160 GN=IPP20_00026 PE=4 SV=1
MM1 pKa = 7.1YY2 pKa = 10.54RR3 pKa = 11.84LDD5 pKa = 3.65IDD7 pKa = 4.2KK8 pKa = 10.89KK9 pKa = 10.96ALKK12 pKa = 9.51QLKK15 pKa = 10.29KK16 pKa = 10.62LDD18 pKa = 3.58TPTRR22 pKa = 11.84KK23 pKa = 9.64QILSWLAKK31 pKa = 10.28NIEE34 pKa = 4.18NTTNPRR40 pKa = 11.84QHH42 pKa = 6.2GKK44 pKa = 9.83ALKK47 pKa = 10.59ANLAGYY53 pKa = 8.08WRR55 pKa = 11.84YY56 pKa = 9.37RR57 pKa = 11.84VEE59 pKa = 4.23NYY61 pKa = 10.43RR62 pKa = 11.84IICDD66 pKa = 3.4IQDD69 pKa = 3.66DD70 pKa = 4.41KK71 pKa = 11.83LVVLAVEE78 pKa = 4.19IAHH81 pKa = 6.81RR82 pKa = 11.84RR83 pKa = 11.84DD84 pKa = 3.19VYY86 pKa = 10.92KK87 pKa = 11.01

Molecular weight: 10.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11900

34

2708

212.5

24.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.084 ± 0.579

0.597 ± 0.111

5.697 ± 0.292

8.151 ± 0.418

4.185 ± 0.34

5.95 ± 0.358

1.504 ± 0.205

7.059 ± 0.319

8.193 ± 0.38

8.613 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.336 ± 0.273

5.849 ± 0.265

2.496 ± 0.234

4.227 ± 0.365

4.714 ± 0.304

6.101 ± 0.586

6.286 ± 0.471

6.185 ± 0.274

1.361 ± 0.111

3.412 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski