Amino acid dipepetide frequency for Streptococcus phage IPP20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.866AlaAla: 3.866 ± 1.335
0.42AlaCys: 0.42 ± 0.217
4.622AlaAsp: 4.622 ± 0.716
5.883AlaGlu: 5.883 ± 0.892
2.941AlaPhe: 2.941 ± 0.47
4.286AlaGly: 4.286 ± 1.073
0.42AlaHis: 0.42 ± 0.217
5.295AlaIle: 5.295 ± 1.016
4.958AlaLys: 4.958 ± 0.538
7.648AlaLeu: 7.648 ± 1.024
1.933AlaMet: 1.933 ± 0.441
4.706AlaAsn: 4.706 ± 0.775
1.765AlaPro: 1.765 ± 0.424
3.11AlaGln: 3.11 ± 0.737
3.11AlaArg: 3.11 ± 0.353
4.454AlaSer: 4.454 ± 1.206
4.79AlaThr: 4.79 ± 0.707
4.034AlaVal: 4.034 ± 0.683
0.924AlaTrp: 0.924 ± 0.254
1.681AlaTyr: 1.681 ± 0.451
0.0AlaXaa: 0.0 ± 0.0
Cys
0.252CysAla: 0.252 ± 0.138
0.0CysCys: 0.0 ± 0.0
0.168CysAsp: 0.168 ± 0.119
0.84CysGlu: 0.84 ± 0.299
0.252CysPhe: 0.252 ± 0.116
0.42CysGly: 0.42 ± 0.224
0.168CysHis: 0.168 ± 0.152
0.42CysIle: 0.42 ± 0.244
0.252CysLys: 0.252 ± 0.161
0.756CysLeu: 0.756 ± 0.323
0.0CysMet: 0.0 ± 0.0
0.084CysAsn: 0.084 ± 0.086
0.084CysPro: 0.084 ± 0.089
0.252CysGln: 0.252 ± 0.134
0.42CysArg: 0.42 ± 0.2
0.168CysSer: 0.168 ± 0.14
0.336CysThr: 0.336 ± 0.203
0.168CysVal: 0.168 ± 0.111
0.252CysTrp: 0.252 ± 0.137
0.672CysTyr: 0.672 ± 0.253
0.0CysXaa: 0.0 ± 0.0
Asp
3.866AspAla: 3.866 ± 0.545
0.168AspCys: 0.168 ± 0.124
3.782AspAsp: 3.782 ± 0.884
3.866AspGlu: 3.866 ± 1.069
2.269AspPhe: 2.269 ± 0.439
4.538AspGly: 4.538 ± 0.76
0.84AspHis: 0.84 ± 0.282
5.211AspIle: 5.211 ± 0.6
5.379AspLys: 5.379 ± 0.914
5.042AspLeu: 5.042 ± 0.745
1.093AspMet: 1.093 ± 0.384
2.857AspAsn: 2.857 ± 0.586
1.429AspPro: 1.429 ± 0.393
1.429AspGln: 1.429 ± 0.252
1.933AspArg: 1.933 ± 0.426
2.857AspSer: 2.857 ± 0.495
2.857AspThr: 2.857 ± 0.461
4.034AspVal: 4.034 ± 0.599
0.924AspTrp: 0.924 ± 0.275
2.605AspTyr: 2.605 ± 0.609
0.0AspXaa: 0.0 ± 0.0
Glu
5.883GluAla: 5.883 ± 1.141
0.42GluCys: 0.42 ± 0.229
4.118GluAsp: 4.118 ± 0.784
6.135GluGlu: 6.135 ± 1.089
3.95GluPhe: 3.95 ± 0.631
3.362GluGly: 3.362 ± 0.582
1.008GluHis: 1.008 ± 0.324
6.891GluIle: 6.891 ± 0.822
7.143GluLys: 7.143 ± 1.038
9.497GluLeu: 9.497 ± 1.105
2.269GluMet: 2.269 ± 0.61
5.379GluAsn: 5.379 ± 0.614
1.177GluPro: 1.177 ± 0.499
3.95GluGln: 3.95 ± 0.877
3.194GluArg: 3.194 ± 0.719
4.202GluSer: 4.202 ± 0.62
4.37GluThr: 4.37 ± 0.813
4.622GluVal: 4.622 ± 0.688
1.261GluTrp: 1.261 ± 0.341
2.605GluTyr: 2.605 ± 0.637
0.0GluXaa: 0.0 ± 0.0
Phe
2.689PheAla: 2.689 ± 0.684
0.252PheCys: 0.252 ± 0.126
3.025PheAsp: 3.025 ± 0.487
2.857PheGlu: 2.857 ± 0.567
1.849PhePhe: 1.849 ± 0.771
2.437PheGly: 2.437 ± 0.593
0.672PheHis: 0.672 ± 0.257
3.278PheIle: 3.278 ± 0.945
4.034PheLys: 4.034 ± 0.471
2.941PheLeu: 2.941 ± 0.56
1.177PheMet: 1.177 ± 0.411
2.857PheAsn: 2.857 ± 0.505
1.093PhePro: 1.093 ± 0.422
1.429PheGln: 1.429 ± 0.356
1.933PheArg: 1.933 ± 0.316
2.185PheSer: 2.185 ± 0.583
2.437PheThr: 2.437 ± 0.36
2.353PheVal: 2.353 ± 0.666
0.504PheTrp: 0.504 ± 0.179
1.849PheTyr: 1.849 ± 0.31
0.0PheXaa: 0.0 ± 0.0
Gly
3.11GlyAla: 3.11 ± 0.644
0.252GlyCys: 0.252 ± 0.135
3.194GlyAsp: 3.194 ± 0.59
3.95GlyGlu: 3.95 ± 0.517
3.11GlyPhe: 3.11 ± 0.599
3.95GlyGly: 3.95 ± 0.497
1.177GlyHis: 1.177 ± 0.363
4.454GlyIle: 4.454 ± 0.569
4.034GlyLys: 4.034 ± 0.485
5.126GlyLeu: 5.126 ± 0.628
1.681GlyMet: 1.681 ± 0.446
3.866GlyAsn: 3.866 ± 0.753
0.84GlyPro: 0.84 ± 0.254
2.437GlyGln: 2.437 ± 0.592
3.278GlyArg: 3.278 ± 0.634
3.446GlySer: 3.446 ± 0.787
3.614GlyThr: 3.614 ± 0.54
3.866GlyVal: 3.866 ± 0.517
1.345GlyTrp: 1.345 ± 0.475
2.773GlyTyr: 2.773 ± 0.453
0.0GlyXaa: 0.0 ± 0.0
His
0.588HisAla: 0.588 ± 0.197
0.252HisCys: 0.252 ± 0.171
0.84HisAsp: 0.84 ± 0.335
1.345HisGlu: 1.345 ± 0.369
0.924HisPhe: 0.924 ± 0.352
0.756HisGly: 0.756 ± 0.31
0.336HisHis: 0.336 ± 0.18
1.849HisIle: 1.849 ± 0.363
0.84HisLys: 0.84 ± 0.245
1.345HisLeu: 1.345 ± 0.38
0.336HisMet: 0.336 ± 0.151
0.84HisAsn: 0.84 ± 0.264
0.336HisPro: 0.336 ± 0.169
0.504HisGln: 0.504 ± 0.225
0.504HisArg: 0.504 ± 0.237
1.093HisSer: 1.093 ± 0.378
0.756HisThr: 0.756 ± 0.28
0.924HisVal: 0.924 ± 0.281
0.0HisTrp: 0.0 ± 0.0
0.672HisTyr: 0.672 ± 0.245
0.0HisXaa: 0.0 ± 0.0
Ile
6.555IleAla: 6.555 ± 0.694
0.756IleCys: 0.756 ± 0.271
4.034IleAsp: 4.034 ± 0.754
5.715IleGlu: 5.715 ± 0.69
2.353IlePhe: 2.353 ± 0.469
4.034IleGly: 4.034 ± 0.611
0.84IleHis: 0.84 ± 0.275
4.706IleIle: 4.706 ± 0.943
5.295IleLys: 5.295 ± 0.761
5.631IleLeu: 5.631 ± 0.86
1.261IleMet: 1.261 ± 0.326
3.866IleAsn: 3.866 ± 0.435
1.933IlePro: 1.933 ± 0.284
2.773IleGln: 2.773 ± 0.403
3.614IleArg: 3.614 ± 0.803
6.387IleSer: 6.387 ± 1.49
4.622IleThr: 4.622 ± 0.664
3.95IleVal: 3.95 ± 0.739
0.756IleTrp: 0.756 ± 0.253
1.513IleTyr: 1.513 ± 0.445
0.0IleXaa: 0.0 ± 0.0
Lys
6.387LysAla: 6.387 ± 0.795
0.168LysCys: 0.168 ± 0.119
5.967LysAsp: 5.967 ± 0.775
7.143LysGlu: 7.143 ± 1.012
2.857LysPhe: 2.857 ± 0.492
4.034LysGly: 4.034 ± 0.643
1.849LysHis: 1.849 ± 0.439
4.958LysIle: 4.958 ± 0.537
5.799LysLys: 5.799 ± 1.033
6.471LysLeu: 6.471 ± 0.661
1.933LysMet: 1.933 ± 0.316
4.874LysAsn: 4.874 ± 0.62
2.353LysPro: 2.353 ± 0.541
4.286LysGln: 4.286 ± 0.604
4.202LysArg: 4.202 ± 0.732
4.538LysSer: 4.538 ± 0.586
4.958LysThr: 4.958 ± 0.589
5.463LysVal: 5.463 ± 0.679
1.093LysTrp: 1.093 ± 0.373
2.605LysTyr: 2.605 ± 0.527
0.0LysXaa: 0.0 ± 0.0
Leu
6.807LeuAla: 6.807 ± 0.614
0.42LeuCys: 0.42 ± 0.255
5.799LeuAsp: 5.799 ± 0.628
7.564LeuGlu: 7.564 ± 0.907
3.95LeuPhe: 3.95 ± 0.572
6.303LeuGly: 6.303 ± 0.55
1.177LeuHis: 1.177 ± 0.323
4.286LeuIle: 4.286 ± 0.496
7.816LeuLys: 7.816 ± 1.01
5.883LeuLeu: 5.883 ± 1.059
2.101LeuMet: 2.101 ± 0.554
5.042LeuAsn: 5.042 ± 0.799
2.353LeuPro: 2.353 ± 0.608
2.605LeuGln: 2.605 ± 0.92
5.211LeuArg: 5.211 ± 0.593
6.135LeuSer: 6.135 ± 0.775
5.715LeuThr: 5.715 ± 0.595
3.866LeuVal: 3.866 ± 0.597
0.672LeuTrp: 0.672 ± 0.194
2.437LeuTyr: 2.437 ± 0.428
0.0LeuXaa: 0.0 ± 0.0
Met
1.681MetAla: 1.681 ± 0.453
0.084MetCys: 0.084 ± 0.073
1.261MetAsp: 1.261 ± 0.418
2.185MetGlu: 2.185 ± 0.543
0.672MetPhe: 0.672 ± 0.32
1.177MetGly: 1.177 ± 0.26
0.084MetHis: 0.084 ± 0.094
1.345MetIle: 1.345 ± 0.293
2.017MetLys: 2.017 ± 0.545
2.017MetLeu: 2.017 ± 0.462
0.588MetMet: 0.588 ± 0.214
2.185MetAsn: 2.185 ± 0.559
0.336MetPro: 0.336 ± 0.147
0.672MetGln: 0.672 ± 0.233
1.261MetArg: 1.261 ± 0.456
1.429MetSer: 1.429 ± 0.285
2.017MetThr: 2.017 ± 0.557
1.681MetVal: 1.681 ± 0.384
0.084MetTrp: 0.084 ± 0.089
0.588MetTyr: 0.588 ± 0.212
0.0MetXaa: 0.0 ± 0.0
Asn
4.034AsnAla: 4.034 ± 0.893
0.168AsnCys: 0.168 ± 0.096
2.269AsnAsp: 2.269 ± 0.507
4.622AsnGlu: 4.622 ± 0.553
2.689AsnPhe: 2.689 ± 0.389
4.286AsnGly: 4.286 ± 0.683
1.513AsnHis: 1.513 ± 0.458
4.286AsnIle: 4.286 ± 0.554
5.379AsnLys: 5.379 ± 0.548
4.37AsnLeu: 4.37 ± 0.573
0.924AsnMet: 0.924 ± 0.428
2.269AsnAsn: 2.269 ± 0.457
2.689AsnPro: 2.689 ± 0.512
3.866AsnGln: 3.866 ± 0.768
2.353AsnArg: 2.353 ± 0.521
3.866AsnSer: 3.866 ± 0.811
2.857AsnThr: 2.857 ± 0.403
4.286AsnVal: 4.286 ± 0.658
0.924AsnTrp: 0.924 ± 0.317
0.84AsnTyr: 0.84 ± 0.218
0.0AsnXaa: 0.0 ± 0.0
Pro
1.681ProAla: 1.681 ± 0.349
0.336ProCys: 0.336 ± 0.165
2.017ProAsp: 2.017 ± 0.572
2.101ProGlu: 2.101 ± 0.507
0.84ProPhe: 0.84 ± 0.295
0.588ProGly: 0.588 ± 0.233
0.588ProHis: 0.588 ± 0.245
1.765ProIle: 1.765 ± 0.46
2.437ProLys: 2.437 ± 0.653
2.353ProLeu: 2.353 ± 0.489
0.168ProMet: 0.168 ± 0.124
0.84ProAsn: 0.84 ± 0.303
0.336ProPro: 0.336 ± 0.232
1.345ProGln: 1.345 ± 0.456
0.924ProArg: 0.924 ± 0.255
1.597ProSer: 1.597 ± 0.391
1.933ProThr: 1.933 ± 0.62
1.933ProVal: 1.933 ± 0.419
0.336ProTrp: 0.336 ± 0.196
0.84ProTyr: 0.84 ± 0.323
0.0ProXaa: 0.0 ± 0.0
Gln
4.706GlnAla: 4.706 ± 1.008
0.168GlnCys: 0.168 ± 0.1
1.513GlnAsp: 1.513 ± 0.366
4.286GlnGlu: 4.286 ± 0.733
1.681GlnPhe: 1.681 ± 0.379
2.101GlnGly: 2.101 ± 0.378
0.42GlnHis: 0.42 ± 0.238
2.605GlnIle: 2.605 ± 0.41
3.446GlnLys: 3.446 ± 0.614
2.773GlnLeu: 2.773 ± 0.581
1.345GlnMet: 1.345 ± 0.35
2.521GlnAsn: 2.521 ± 0.366
1.261GlnPro: 1.261 ± 0.368
1.177GlnGln: 1.177 ± 0.411
2.101GlnArg: 2.101 ± 0.52
3.362GlnSer: 3.362 ± 0.445
2.773GlnThr: 2.773 ± 0.561
2.521GlnVal: 2.521 ± 0.482
0.336GlnTrp: 0.336 ± 0.195
1.177GlnTyr: 1.177 ± 0.411
0.0GlnXaa: 0.0 ± 0.0
Arg
3.866ArgAla: 3.866 ± 0.624
0.252ArgCys: 0.252 ± 0.139
2.101ArgAsp: 2.101 ± 0.453
3.95ArgGlu: 3.95 ± 0.733
1.513ArgPhe: 1.513 ± 0.405
2.353ArgGly: 2.353 ± 0.515
0.504ArgHis: 0.504 ± 0.254
3.446ArgIle: 3.446 ± 0.566
3.782ArgLys: 3.782 ± 0.757
4.706ArgLeu: 4.706 ± 0.756
1.177ArgMet: 1.177 ± 0.288
3.446ArgAsn: 3.446 ± 0.465
1.345ArgPro: 1.345 ± 0.376
2.269ArgGln: 2.269 ± 0.76
2.941ArgArg: 2.941 ± 0.529
1.681ArgSer: 1.681 ± 0.372
2.605ArgThr: 2.605 ± 0.794
2.185ArgVal: 2.185 ± 0.436
0.756ArgTrp: 0.756 ± 0.259
2.269ArgTyr: 2.269 ± 0.619
0.0ArgXaa: 0.0 ± 0.0
Ser
4.706SerAla: 4.706 ± 0.912
0.588SerCys: 0.588 ± 0.223
3.025SerAsp: 3.025 ± 0.655
5.379SerGlu: 5.379 ± 1.004
2.857SerPhe: 2.857 ± 0.471
4.286SerGly: 4.286 ± 1.068
0.924SerHis: 0.924 ± 0.274
3.362SerIle: 3.362 ± 0.443
5.042SerLys: 5.042 ± 0.735
4.37SerLeu: 4.37 ± 0.58
1.429SerMet: 1.429 ± 0.516
3.446SerAsn: 3.446 ± 0.593
1.681SerPro: 1.681 ± 0.308
2.185SerGln: 2.185 ± 0.694
2.605SerArg: 2.605 ± 0.609
3.866SerSer: 3.866 ± 0.629
3.95SerThr: 3.95 ± 1.158
4.034SerVal: 4.034 ± 0.828
0.924SerTrp: 0.924 ± 0.231
2.353SerTyr: 2.353 ± 0.462
0.0SerXaa: 0.0 ± 0.0
Thr
4.622ThrAla: 4.622 ± 1.308
0.252ThrCys: 0.252 ± 0.17
4.286ThrAsp: 4.286 ± 0.704
4.286ThrGlu: 4.286 ± 0.681
3.194ThrPhe: 3.194 ± 0.748
3.698ThrGly: 3.698 ± 0.663
0.756ThrHis: 0.756 ± 0.267
5.042ThrIle: 5.042 ± 0.634
5.042ThrLys: 5.042 ± 0.686
5.211ThrLeu: 5.211 ± 0.605
1.093ThrMet: 1.093 ± 0.407
2.857ThrAsn: 2.857 ± 0.543
0.84ThrPro: 0.84 ± 0.29
3.11ThrGln: 3.11 ± 0.85
2.605ThrArg: 2.605 ± 0.469
3.866ThrSer: 3.866 ± 0.686
4.706ThrThr: 4.706 ± 0.859
4.79ThrVal: 4.79 ± 0.802
0.504ThrTrp: 0.504 ± 0.203
2.101ThrTyr: 2.101 ± 0.507
0.0ThrXaa: 0.0 ± 0.0
Val
2.941ValAla: 2.941 ± 0.464
0.252ValCys: 0.252 ± 0.144
3.278ValAsp: 3.278 ± 0.532
5.715ValGlu: 5.715 ± 0.707
2.017ValPhe: 2.017 ± 0.48
4.37ValGly: 4.37 ± 0.602
0.924ValHis: 0.924 ± 0.245
4.286ValIle: 4.286 ± 0.574
6.303ValLys: 6.303 ± 0.663
4.874ValLeu: 4.874 ± 0.947
1.429ValMet: 1.429 ± 0.393
3.362ValAsn: 3.362 ± 0.447
1.429ValPro: 1.429 ± 0.431
2.101ValGln: 2.101 ± 0.429
1.933ValArg: 1.933 ± 0.555
3.446ValSer: 3.446 ± 0.584
5.295ValThr: 5.295 ± 0.769
4.874ValVal: 4.874 ± 0.722
1.177ValTrp: 1.177 ± 0.364
1.849ValTyr: 1.849 ± 0.526
0.0ValXaa: 0.0 ± 0.0
Trp
0.756TrpAla: 0.756 ± 0.267
0.168TrpCys: 0.168 ± 0.132
0.336TrpAsp: 0.336 ± 0.157
1.177TrpGlu: 1.177 ± 0.397
0.588TrpPhe: 0.588 ± 0.26
0.672TrpGly: 0.672 ± 0.237
0.252TrpHis: 0.252 ± 0.164
1.008TrpIle: 1.008 ± 0.33
0.588TrpLys: 0.588 ± 0.235
1.093TrpLeu: 1.093 ± 0.273
0.504TrpMet: 0.504 ± 0.22
1.513TrpAsn: 1.513 ± 0.356
0.168TrpPro: 0.168 ± 0.1
1.093TrpGln: 1.093 ± 0.237
0.672TrpArg: 0.672 ± 0.315
0.756TrpSer: 0.756 ± 0.277
0.84TrpThr: 0.84 ± 0.306
0.504TrpVal: 0.504 ± 0.185
0.252TrpTrp: 0.252 ± 0.11
0.672TrpTyr: 0.672 ± 0.55
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.849TyrAla: 1.849 ± 0.337
0.588TyrCys: 0.588 ± 0.231
1.345TyrAsp: 1.345 ± 0.265
2.521TyrGlu: 2.521 ± 0.481
1.345TyrPhe: 1.345 ± 0.448
1.849TyrGly: 1.849 ± 0.442
0.588TyrHis: 0.588 ± 0.182
2.101TyrIle: 2.101 ± 0.369
2.353TyrLys: 2.353 ± 0.479
4.034TyrLeu: 4.034 ± 0.69
0.84TyrMet: 0.84 ± 0.363
1.681TyrAsn: 1.681 ± 0.521
1.513TyrPro: 1.513 ± 0.349
1.681TyrGln: 1.681 ± 0.437
2.353TyrArg: 2.353 ± 0.668
1.681TyrSer: 1.681 ± 0.391
1.429TyrThr: 1.429 ± 0.306
1.765TyrVal: 1.765 ± 0.379
0.588TyrTrp: 0.588 ± 0.194
2.017TyrTyr: 2.017 ± 0.731
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (11900 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski