Amphiprion ocellaris (Clown anemonefish)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia; Ctenosquamata; Acanthomorphata;

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31745 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q1CDX7|A0A3Q1CDX7_AMPOC Hydroxycarboxylic acid receptor 1-3 OS=Amphiprion ocellaris OX=80972 PE=3 SV=1
MM1 pKa = 7.66LCTPSILDD9 pKa = 3.94HH10 pKa = 7.09PPSPMYY16 pKa = 9.87TFSVTAEE23 pKa = 4.12EE24 pKa = 4.22EE25 pKa = 4.04QGLDD29 pKa = 3.69GMDD32 pKa = 3.03WLDD35 pKa = 3.58FTSGRR40 pKa = 11.84EE41 pKa = 3.94KK42 pKa = 11.09DD43 pKa = 3.85EE44 pKa = 4.53EE45 pKa = 4.55PPTLAPLAPQTPSSVFSTDD64 pKa = 3.55FLDD67 pKa = 6.07GYY69 pKa = 10.27DD70 pKa = 3.65LHH72 pKa = 8.36IHH74 pKa = 6.97LDD76 pKa = 3.51SCLL79 pKa = 3.42

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q1B719|A0A3Q1B719_AMPOC Ribosomal protein L7 OS=Amphiprion ocellaris OX=80972 PE=3 SV=1
MM1 pKa = 6.59EE2 pKa = 4.24QFYY5 pKa = 10.8IEE7 pKa = 4.4CTRR10 pKa = 11.84GSSTPPRR17 pKa = 11.84GSSTPSRR24 pKa = 11.84GSSAPPRR31 pKa = 11.84GSSAPSRR38 pKa = 11.84QSSTPPRR45 pKa = 11.84QSSTPPRR52 pKa = 11.84GSSTPPRR59 pKa = 11.84GSSTPSRR66 pKa = 11.84GSSTPPRR73 pKa = 11.84GSSTPSRR80 pKa = 11.84GSSTPPRR87 pKa = 11.84GSSTPPRR94 pKa = 11.84GSSTPPRR101 pKa = 11.84GSSTPPRR108 pKa = 11.84GSSTPPRR115 pKa = 11.84GSSTPSRR122 pKa = 11.84QSSTPPRR129 pKa = 11.84GSSTPSRR136 pKa = 11.84QSSTPPRR143 pKa = 11.84GSSTPSRR150 pKa = 11.84QSSTPPRR157 pKa = 11.84GSSTPPRR164 pKa = 11.84GSSTPSRR171 pKa = 11.84QSSTPPRR178 pKa = 11.84GSSTPPRR185 pKa = 11.84RR186 pKa = 11.84SSTPPRR192 pKa = 11.84GSSTPPRR199 pKa = 11.84GSSAPSRR206 pKa = 11.84QSSTPPRR213 pKa = 11.84GSSAPTRR220 pKa = 11.84GSSTPSRR227 pKa = 11.84GSSTPPRR234 pKa = 11.84GSSSPPRR241 pKa = 11.84GSSTPSRR248 pKa = 11.84GSSTPPRR255 pKa = 11.84GSSTPSRR262 pKa = 11.84GSSTPPRR269 pKa = 11.84GSSTPPRR276 pKa = 11.84GSSTPPRR283 pKa = 11.84GSSTPPRR290 pKa = 11.84GSSTPPRR297 pKa = 11.84GSSTPSRR304 pKa = 11.84QSSTPPRR311 pKa = 11.84GSSTPSRR318 pKa = 11.84QSSTPPRR325 pKa = 11.84GSSTPSRR332 pKa = 11.84QSSTPPRR339 pKa = 11.84GSSTPPRR346 pKa = 11.84GSSTPSRR353 pKa = 11.84QSSTPPRR360 pKa = 11.84GSSTPPRR367 pKa = 11.84RR368 pKa = 11.84SSTPPRR374 pKa = 11.84GSSTPPRR381 pKa = 11.84GSSAPSRR388 pKa = 11.84QSSTPPRR395 pKa = 11.84GSSTPPRR402 pKa = 4.56

Molecular weight: 40.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23429

8316

31745

17832489

17

7970

561.7

62.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.525 ± 0.011

2.247 ± 0.011

5.234 ± 0.01

6.826 ± 0.018

3.66 ± 0.011

6.29 ± 0.013

2.655 ± 0.008

4.405 ± 0.011

5.601 ± 0.015

9.524 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.006

3.831 ± 0.007

5.697 ± 0.02

4.769 ± 0.012

5.625 ± 0.012

8.755 ± 0.02

5.658 ± 0.011

6.386 ± 0.013

1.147 ± 0.005

2.773 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski