Amino acid dipepetide frequency for Amphiprion ocellaris (Clown anemonefish)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.376AlaAla: 6.376 ± 0.033	1.248AlaCys: 1.248 ± 0.01	3.167AlaAsp: 3.167 ± 0.015	4.652AlaGlu: 4.652 ± 0.024	2.388AlaPhe: 2.388 ± 0.013	4.317AlaGly: 4.317 ± 0.022	1.469AlaHis: 1.469 ± 0.011	2.682AlaIle: 2.682 ± 0.013	3.348AlaLys: 3.348 ± 0.017	6.388AlaLeu: 6.388 ± 0.029	1.595AlaMet: 1.595 ± 0.009	2.172AlaAsn: 2.172 ± 0.012	3.465AlaPro: 3.465 ± 0.022	2.915AlaGln: 2.915 ± 0.016	3.012AlaArg: 3.012 ± 0.014	5.468AlaSer: 5.468 ± 0.024	3.52AlaThr: 3.52 ± 0.02	4.928AlaVal: 4.928 ± 0.02	0.639AlaTrp: 0.639 ± 0.006	1.504AlaTyr: 1.504 ± 0.011	0.0AlaXaa: 0.0 ± 0.0
Cys	1.131CysAla: 1.131 ± 0.01	0.673CysCys: 0.673 ± 0.009	1.141CysAsp: 1.141 ± 0.013	1.248CysGlu: 1.248 ± 0.012	0.923CysPhe: 0.923 ± 0.007	1.576CysGly: 1.576 ± 0.016	0.663CysHis: 0.663 ± 0.008	0.988CysIle: 0.988 ± 0.008	1.146CysLys: 1.146 ± 0.011	2.208CysLeu: 2.208 ± 0.015	0.484CysMet: 0.484 ± 0.005	0.836CysAsn: 0.836 ± 0.009	1.277CysPro: 1.277 ± 0.013	1.037CysGln: 1.037 ± 0.011	1.298CysArg: 1.298 ± 0.011	2.177CysSer: 2.177 ± 0.015	1.167CysThr: 1.167 ± 0.012	1.541CysVal: 1.541 ± 0.012	0.316CysTrp: 0.316 ± 0.004	0.641CysTyr: 0.641 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	2.969AspAla: 2.969 ± 0.016	1.146AspCys: 1.146 ± 0.011	3.161AspAsp: 3.161 ± 0.02	3.781AspGlu: 3.781 ± 0.022	2.123AspPhe: 2.123 ± 0.012	3.713AspGly: 3.713 ± 0.019	1.212AspHis: 1.212 ± 0.009	2.694AspIle: 2.694 ± 0.024	2.777AspLys: 2.777 ± 0.016	4.933AspLeu: 4.933 ± 0.02	1.32AspMet: 1.32 ± 0.009	1.983AspAsn: 1.983 ± 0.013	2.843AspPro: 2.843 ± 0.016	2.006AspGln: 2.006 ± 0.013	2.844AspArg: 2.844 ± 0.017	4.57AspSer: 4.57 ± 0.023	2.656AspThr: 2.656 ± 0.013	3.363AspVal: 3.363 ± 0.02	0.685AspTrp: 0.685 ± 0.007	1.566AspTyr: 1.566 ± 0.011	0.0AspXaa: 0.0 ± 0.0
Glu	4.782GluAla: 4.782 ± 0.027	1.21GluCys: 1.21 ± 0.013	4.452GluAsp: 4.452 ± 0.019	7.806GluGlu: 7.806 ± 0.048	1.938GluPhe: 1.938 ± 0.011	4.076GluGly: 4.076 ± 0.021	1.435GluHis: 1.435 ± 0.011	2.822GluIle: 2.822 ± 0.017	4.689GluLys: 4.689 ± 0.03	6.118GluLeu: 6.118 ± 0.028	1.744GluMet: 1.744 ± 0.011	2.747GluAsn: 2.747 ± 0.015	2.81GluPro: 2.81 ± 0.019	3.115GluGln: 3.115 ± 0.02	4.232GluArg: 4.232 ± 0.026	4.296GluSer: 4.296 ± 0.019	3.427GluThr: 3.427 ± 0.016	4.315GluVal: 4.315 ± 0.019	0.666GluTrp: 0.666 ± 0.006	1.581GluTyr: 1.581 ± 0.011	0.0GluXaa: 0.0 ± 0.0
Phe	1.932PheAla: 1.932 ± 0.013	0.996PheCys: 0.996 ± 0.009	1.788PheAsp: 1.788 ± 0.011	1.854PheGlu: 1.854 ± 0.011	1.657PhePhe: 1.657 ± 0.015	2.196PheGly: 2.196 ± 0.013	1.043PheHis: 1.043 ± 0.008	1.962PheIle: 1.962 ± 0.015	1.795PheLys: 1.795 ± 0.013	3.925PheLeu: 3.925 ± 0.021	0.855PheMet: 0.855 ± 0.008	1.499PheAsn: 1.499 ± 0.011	1.823PhePro: 1.823 ± 0.01	1.622PheGln: 1.622 ± 0.01	1.882PheArg: 1.882 ± 0.011	3.484PheSer: 3.484 ± 0.019	2.324PheThr: 2.324 ± 0.016	2.254PheVal: 2.254 ± 0.014	0.475PheTrp: 0.475 ± 0.006	1.234PheTyr: 1.234 ± 0.009	0.0PheXaa: 0.0 ± 0.0
Gly	3.937GlyAla: 3.937 ± 0.024	1.253GlyCys: 1.253 ± 0.011	3.223GlyAsp: 3.223 ± 0.016	4.053GlyGlu: 4.053 ± 0.022	2.43GlyPhe: 2.43 ± 0.015	5.48GlyGly: 5.48 ± 0.037	1.652GlyHis: 1.652 ± 0.012	2.611GlyIle: 2.611 ± 0.016	3.572GlyLys: 3.572 ± 0.02	5.437GlyLeu: 5.437 ± 0.023	1.481GlyMet: 1.481 ± 0.011	2.454GlyAsn: 2.454 ± 0.014	3.242GlyPro: 3.242 ± 0.038	2.736GlyGln: 2.736 ± 0.016	3.637GlyArg: 3.637 ± 0.02	5.738GlySer: 5.738 ± 0.022	3.375GlyThr: 3.375 ± 0.017	3.999GlyVal: 3.999 ± 0.018	0.772GlyTrp: 0.772 ± 0.009	1.823GlyTyr: 1.823 ± 0.012	0.0GlyXaa: 0.0 ± 0.0
His	1.322HisAla: 1.322 ± 0.009	0.77HisCys: 0.77 ± 0.008	0.988HisAsp: 0.988 ± 0.007	1.198HisGlu: 1.198 ± 0.009	1.072HisPhe: 1.072 ± 0.008	1.557HisGly: 1.557 ± 0.012	1.124HisHis: 1.124 ± 0.013	1.329HisIle: 1.329 ± 0.009	1.307HisLys: 1.307 ± 0.01	2.719HisLeu: 2.719 ± 0.014	0.711HisMet: 0.711 ± 0.009	1.047HisAsn: 1.047 ± 0.009	1.62HisPro: 1.62 ± 0.014	1.304HisGln: 1.304 ± 0.012	1.657HisArg: 1.657 ± 0.011	2.539HisSer: 2.539 ± 0.015	1.646HisThr: 1.646 ± 0.013	1.424HisVal: 1.424 ± 0.009	0.341HisTrp: 0.341 ± 0.004	0.87HisTyr: 0.87 ± 0.008	0.0HisXaa: 0.0 ± 0.0
Ile	2.477IleAla: 2.477 ± 0.013	1.098IleCys: 1.098 ± 0.009	2.118IleAsp: 2.118 ± 0.014	2.362IleGlu: 2.362 ± 0.014	1.838IlePhe: 1.838 ± 0.013	2.247IleGly: 2.247 ± 0.013	1.304IleHis: 1.304 ± 0.009	2.433IleIle: 2.433 ± 0.016	2.519IleLys: 2.519 ± 0.014	4.295IleLeu: 4.295 ± 0.021	1.088IleMet: 1.088 ± 0.01	1.959IleAsn: 1.959 ± 0.012	2.452IlePro: 2.452 ± 0.011	2.212IleGln: 2.212 ± 0.012	2.453IleArg: 2.453 ± 0.014	3.852IleSer: 3.852 ± 0.019	2.849IleThr: 2.849 ± 0.015	2.593IleVal: 2.593 ± 0.015	0.476IleTrp: 0.476 ± 0.005	1.423IleTyr: 1.423 ± 0.009	0.0IleXaa: 0.0 ± 0.0
Lys	3.725LysAla: 3.725 ± 0.022	1.021LysCys: 1.021 ± 0.009	3.256LysAsp: 3.256 ± 0.018	4.693LysGlu: 4.693 ± 0.026	1.595LysPhe: 1.595 ± 0.012	3.105LysGly: 3.105 ± 0.022	1.417LysHis: 1.417 ± 0.009	2.453LysIle: 2.453 ± 0.014	4.427LysLys: 4.427 ± 0.032	4.972LysLeu: 4.972 ± 0.021	1.492LysMet: 1.492 ± 0.011	2.256LysAsn: 2.256 ± 0.013	2.969LysPro: 2.969 ± 0.02	2.588LysGln: 2.588 ± 0.017	3.38LysArg: 3.38 ± 0.017	3.818LysSer: 3.818 ± 0.019	3.265LysThr: 3.265 ± 0.021	3.524LysVal: 3.524 ± 0.015	0.555LysTrp: 0.555 ± 0.005	1.497LysTyr: 1.497 ± 0.011	0.0LysXaa: 0.0 ± 0.0
Leu	5.946LeuAla: 5.946 ± 0.023	2.249LeuCys: 2.249 ± 0.015	4.907LeuAsp: 4.907 ± 0.017	6.352LeuGlu: 6.352 ± 0.035	3.467LeuPhe: 3.467 ± 0.019	5.078LeuGly: 5.078 ± 0.018	2.745LeuHis: 2.745 ± 0.014	3.851LeuIle: 3.851 ± 0.019	5.565LeuLys: 5.565 ± 0.024	10.104LeuLeu: 10.104 ± 0.047	2.138LeuMet: 2.138 ± 0.011	3.64LeuAsn: 3.64 ± 0.017	5.297LeuPro: 5.297 ± 0.021	5.546LeuGln: 5.546 ± 0.028	5.569LeuArg: 5.569 ± 0.023	8.316LeuSer: 8.316 ± 0.03	5.268LeuThr: 5.268 ± 0.021	5.498LeuVal: 5.498 ± 0.024	1.07LeuTrp: 1.07 ± 0.009	2.633LeuTyr: 2.633 ± 0.014	0.0LeuXaa: 0.0 ± 0.0
Met	1.918MetAla: 1.918 ± 0.013	0.493MetCys: 0.493 ± 0.006	1.47MetAsp: 1.47 ± 0.01	2.001MetGlu: 2.001 ± 0.012	0.892MetPhe: 0.892 ± 0.008	1.423MetGly: 1.423 ± 0.011	0.497MetHis: 0.497 ± 0.006	0.888MetIle: 0.888 ± 0.007	1.528MetLys: 1.528 ± 0.011	2.122MetLeu: 2.122 ± 0.012	0.727MetMet: 0.727 ± 0.007	0.926MetAsn: 0.926 ± 0.008	1.095MetPro: 1.095 ± 0.014	1.011MetGln: 1.011 ± 0.007	1.223MetArg: 1.223 ± 0.009	1.927MetSer: 1.927 ± 0.012	1.302MetThr: 1.302 ± 0.009	1.559MetVal: 1.559 ± 0.011	0.272MetTrp: 0.272 ± 0.004	0.644MetTyr: 0.644 ± 0.006	0.0MetXaa: 0.0 ± 0.0
Asn	2.163AsnAla: 2.163 ± 0.015	0.861AsnCys: 0.861 ± 0.008	1.698AsnAsp: 1.698 ± 0.013	2.03AsnGlu: 2.03 ± 0.014	1.422AsnPhe: 1.422 ± 0.01	2.751AsnGly: 2.751 ± 0.018	1.024AsnHis: 1.024 ± 0.008	2.168AsnIle: 2.168 ± 0.013	2.209AsnLys: 2.209 ± 0.013	3.636AsnLeu: 3.636 ± 0.019	1.076AsnMet: 1.076 ± 0.008	1.793AsnAsn: 1.793 ± 0.014	2.243AsnPro: 2.243 ± 0.013	1.806AsnGln: 1.806 ± 0.011	1.983AsnArg: 1.983 ± 0.011	3.268AsnSer: 3.268 ± 0.016	2.298AsnThr: 2.298 ± 0.015	2.304AsnVal: 2.304 ± 0.014	0.44AsnTrp: 0.44 ± 0.005	1.141AsnTyr: 1.141 ± 0.01	0.0AsnXaa: 0.0 ± 0.0
Pro	4.183ProAla: 4.183 ± 0.025	1.042ProCys: 1.042 ± 0.011	2.924ProAsp: 2.924 ± 0.02	3.776ProGlu: 3.776 ± 0.018	1.795ProPhe: 1.795 ± 0.013	4.008ProGly: 4.008 ± 0.041	1.531ProHis: 1.531 ± 0.012	1.89ProIle: 1.89 ± 0.013	2.522ProLys: 2.522 ± 0.025	4.81ProLeu: 4.81 ± 0.02	1.033ProMet: 1.033 ± 0.008	1.925ProAsn: 1.925 ± 0.012	5.547ProPro: 5.547 ± 0.043	2.713ProGln: 2.713 ± 0.021	2.734ProArg: 2.734 ± 0.015	5.606ProSer: 5.606 ± 0.032	3.122ProThr: 3.122 ± 0.021	3.8ProVal: 3.8 ± 0.02	0.543ProTrp: 0.543 ± 0.007	1.465ProTyr: 1.465 ± 0.012	0.0ProXaa: 0.0 ± 0.0
Gln	3.148GlnAla: 3.148 ± 0.018	0.968GlnCys: 0.968 ± 0.012	2.366GlnAsp: 2.366 ± 0.014	3.502GlnGlu: 3.502 ± 0.019	1.366GlnPhe: 1.366 ± 0.009	2.717GlnGly: 2.717 ± 0.015	1.401GlnHis: 1.401 ± 0.012	1.93GlnIle: 1.93 ± 0.012	2.647GlnLys: 2.647 ± 0.018	4.561GlnLeu: 4.561 ± 0.025	1.17GlnMet: 1.17 ± 0.008	1.812GlnAsn: 1.812 ± 0.012	2.647GlnPro: 2.647 ± 0.019	3.515GlnGln: 3.515 ± 0.035	3.123GlnArg: 3.123 ± 0.016	3.534GlnSer: 3.534 ± 0.019	2.654GlnThr: 2.654 ± 0.014	2.831GlnVal: 2.831 ± 0.015	0.539GlnTrp: 0.539 ± 0.006	1.256GlnTyr: 1.256 ± 0.01	0.0GlnXaa: 0.0 ± 0.0
Arg	3.329ArgAla: 3.329 ± 0.015	1.28ArgCys: 1.28 ± 0.012	2.94ArgAsp: 2.94 ± 0.015	3.89ArgGlu: 3.89 ± 0.025	1.98ArgPhe: 1.98 ± 0.011	3.458ArgGly: 3.458 ± 0.022	1.591ArgHis: 1.591 ± 0.012	2.393ArgIle: 2.393 ± 0.014	3.512ArgLys: 3.512 ± 0.017	5.321ArgLeu: 5.321 ± 0.024	1.317ArgMet: 1.317 ± 0.009	2.114ArgAsn: 2.114 ± 0.011	2.957ArgPro: 2.957 ± 0.018	2.71ArgGln: 2.71 ± 0.017	4.422ArgArg: 4.422 ± 0.024	4.542ArgSer: 4.542 ± 0.024	2.976ArgThr: 2.976 ± 0.014	3.271ArgVal: 3.271 ± 0.016	0.682ArgTrp: 0.682 ± 0.008	1.571ArgTyr: 1.571 ± 0.01	0.0ArgXaa: 0.0 ± 0.0
Ser	5.6SerAla: 5.6 ± 0.021	2.052SerCys: 2.052 ± 0.016	4.432SerAsp: 4.432 ± 0.022	4.897SerGlu: 4.897 ± 0.021	3.193SerPhe: 3.193 ± 0.016	5.63SerGly: 5.63 ± 0.023	2.284SerHis: 2.284 ± 0.015	3.411SerIle: 3.411 ± 0.015	3.977SerLys: 3.977 ± 0.02	8.319SerLeu: 8.319 ± 0.031	1.849SerMet: 1.849 ± 0.01	2.997SerAsn: 2.997 ± 0.015	6.015SerPro: 6.015 ± 0.04	3.917SerGln: 3.917 ± 0.017	4.579SerArg: 4.579 ± 0.021	10.695SerSer: 10.695 ± 0.049	4.895SerThr: 4.895 ± 0.027	5.57SerVal: 5.57 ± 0.02	0.993SerTrp: 0.993 ± 0.009	2.238SerTyr: 2.238 ± 0.013	0.0SerXaa: 0.0 ± 0.0
Thr	4.081ThrAla: 4.081 ± 0.019	1.382ThrCys: 1.382 ± 0.013	3.004ThrAsp: 3.004 ± 0.017	3.781ThrGlu: 3.781 ± 0.016	2.127ThrPhe: 2.127 ± 0.012	3.746ThrGly: 3.746 ± 0.017	1.459ThrHis: 1.459 ± 0.014	2.446ThrIle: 2.446 ± 0.014	2.699ThrLys: 2.699 ± 0.015	5.336ThrLeu: 5.336 ± 0.02	1.234ThrMet: 1.234 ± 0.009	1.966ThrAsn: 1.966 ± 0.012	3.617ThrPro: 3.617 ± 0.024	2.378ThrGln: 2.378 ± 0.013	2.489ThrArg: 2.489 ± 0.011	4.915ThrSer: 4.915 ± 0.023	3.5ThrThr: 3.5 ± 0.048	4.294ThrVal: 4.294 ± 0.019	0.665ThrTrp: 0.665 ± 0.006	1.459ThrTyr: 1.459 ± 0.01	0.0ThrXaa: 0.0 ± 0.0
Val	4.147ValAla: 4.147 ± 0.02	1.751ValCys: 1.751 ± 0.013	3.275ValAsp: 3.275 ± 0.018	4.061ValGlu: 4.061 ± 0.019	2.718ValPhe: 2.718 ± 0.017	3.525ValGly: 3.525 ± 0.015	1.607ValHis: 1.607 ± 0.011	3.037ValIle: 3.037 ± 0.016	3.616ValLys: 3.616 ± 0.018	6.228ValLeu: 6.228 ± 0.026	1.563ValMet: 1.563 ± 0.01	2.46ValAsn: 2.46 ± 0.013	3.271ValPro: 3.271 ± 0.018	2.807ValGln: 2.807 ± 0.014	3.297ValArg: 3.297 ± 0.016	5.412ValSer: 5.412 ± 0.021	4.001ValThr: 4.001 ± 0.021	4.496ValVal: 4.496 ± 0.018	0.761ValTrp: 0.761 ± 0.007	1.831ValTyr: 1.831 ± 0.011	0.0ValXaa: 0.0 ± 0.0
Trp	0.658TrpAla: 0.658 ± 0.008	0.249TrpCys: 0.249 ± 0.004	0.643TrpAsp: 0.643 ± 0.007	0.732TrpGlu: 0.732 ± 0.007	0.464TrpPhe: 0.464 ± 0.006	0.626TrpGly: 0.626 ± 0.008	0.279TrpHis: 0.279 ± 0.004	0.565TrpIle: 0.565 ± 0.007	0.699TrpLys: 0.699 ± 0.007	1.141TrpLeu: 1.141 ± 0.01	0.354TrpMet: 0.354 ± 0.004	0.51TrpAsn: 0.51 ± 0.005	0.428TrpPro: 0.428 ± 0.005	0.489TrpGln: 0.489 ± 0.006	0.744TrpArg: 0.744 ± 0.007	0.958TrpSer: 0.958 ± 0.009	0.704TrpThr: 0.704 ± 0.007	0.69TrpVal: 0.69 ± 0.007	0.194TrpTrp: 0.194 ± 0.004	0.344TrpTyr: 0.344 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.43TyrAla: 1.43 ± 0.01	0.729TyrCys: 0.729 ± 0.008	1.391TyrAsp: 1.391 ± 0.009	1.591TyrGlu: 1.591 ± 0.011	1.213TyrPhe: 1.213 ± 0.011	1.678TyrGly: 1.678 ± 0.012	0.808TyrHis: 0.808 ± 0.008	1.49TyrIle: 1.49 ± 0.01	1.457TyrLys: 1.457 ± 0.012	2.663TyrLeu: 2.663 ± 0.014	0.691TyrMet: 0.691 ± 0.007	1.22TyrAsn: 1.22 ± 0.01	1.346TyrPro: 1.346 ± 0.013	1.264TyrGln: 1.264 ± 0.009	1.696TyrArg: 1.696 ± 0.012	2.43TyrSer: 2.43 ± 0.014	1.626TyrThr: 1.626 ± 0.011	1.608TyrVal: 1.608 ± 0.011	0.391TyrTrp: 0.391 ± 0.006	1.009TyrTyr: 1.009 ± 0.009	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 31745 proteins (17832489 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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