Sphingorhabdus contaminans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingorhabdus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3099 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A553WL52|A0A553WL52_9SPHN WalW protein OS=Sphingorhabdus contaminans OX=1343899 GN=FOM92_04810 PE=4 SV=1
MM1 pKa = 7.57LSCGLPASAFAQTVNQYY18 pKa = 9.68TNSTTGNIVDD28 pKa = 4.16STTCATTVTRR38 pKa = 11.84TFTVGTNFTVADD50 pKa = 3.83VNLGVLLSHH59 pKa = 7.12TYY61 pKa = 10.55RR62 pKa = 11.84SDD64 pKa = 3.25LRR66 pKa = 11.84ITLTSPLGTSVQIMTNVGGAADD88 pKa = 3.93NLNVLFDD95 pKa = 4.77DD96 pKa = 4.54EE97 pKa = 4.14AAAAISSHH105 pKa = 5.25STNDD109 pKa = 3.05TTGAVPPYY117 pKa = 10.3LRR119 pKa = 11.84TFRR122 pKa = 11.84PTAALTAFDD131 pKa = 4.48GQSSLGTWTMVICDD145 pKa = 4.06SVLADD150 pKa = 3.11VGTFTRR156 pKa = 11.84ADD158 pKa = 3.89LYY160 pKa = 10.32LTSAPASYY168 pKa = 11.52ADD170 pKa = 4.0LSLTKK175 pKa = 9.73TVSNANPASGGAISYY190 pKa = 7.59TLSVTNSASSPTAASGVTVLDD211 pKa = 3.53ILPAGVSFVSASGFGSYY228 pKa = 10.95DD229 pKa = 3.08SATGVWTVGSIPAGTTRR246 pKa = 11.84TLTINVTVTAGLGTTIQNDD265 pKa = 4.09TEE267 pKa = 4.24ISASSVADD275 pKa = 4.08LDD277 pKa = 3.97STPNNASINEE287 pKa = 4.18DD288 pKa = 3.11DD289 pKa = 4.4DD290 pKa = 4.62AAVSFTTTGTRR301 pKa = 11.84TAGTPPTLVCPNGTTLLDD319 pKa = 3.43WNAQSWTSGSSTGTATVANIGAIGFNVTTQGTFTIPLQLNATITGGTTGQNSLYY373 pKa = 10.79QNVEE377 pKa = 3.86YY378 pKa = 7.71TTRR381 pKa = 11.84TQTTTTVVTLPTAVPGAQFTIFDD404 pKa = 3.52VDD406 pKa = 3.55YY407 pKa = 10.86APNDD411 pKa = 3.63FADD414 pKa = 3.7KK415 pKa = 8.63MTITGTYY422 pKa = 9.07NGASVTPTLTNGVTNYY438 pKa = 9.87VFGNVAIGDD447 pKa = 3.86VTASDD452 pKa = 3.67TTNQGTVVATFSSPVDD468 pKa = 3.71TITIVYY474 pKa = 9.38GNHH477 pKa = 4.23TTAPADD483 pKa = 3.71PDD485 pKa = 3.71GQAIAINDD493 pKa = 3.6FTFCNPQTTLSVTKK507 pKa = 10.47LSNVVSDD514 pKa = 4.59GVSGSNPKK522 pKa = 9.86SVPGAVVRR530 pKa = 11.84YY531 pKa = 8.7CVLVSNSGSATSSNVVATDD550 pKa = 4.21SIPANLTFVPGSMLSGTSCAAATTAEE576 pKa = 4.65DD577 pKa = 4.49DD578 pKa = 4.06NNSGGDD584 pKa = 3.39EE585 pKa = 4.1SDD587 pKa = 3.51PFGMDD592 pKa = 3.43FAGNVVTGRR601 pKa = 11.84AASLGPAASFAMVFNTTVNN620 pKa = 3.29

Molecular weight: 62.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A553WKC7|A0A553WKC7_9SPHN Sodium/solute symporter OS=Sphingorhabdus contaminans OX=1343899 GN=FOM92_07315 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTASGRR28 pKa = 11.84AILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3099

0

3099

1006683

40

3552

324.8

35.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.058 ± 0.066

0.842 ± 0.016

5.928 ± 0.035

5.531 ± 0.047

3.827 ± 0.03

8.646 ± 0.068

1.975 ± 0.025

5.647 ± 0.029

3.905 ± 0.036

9.506 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.582 ± 0.026

3.204 ± 0.045

4.959 ± 0.038

3.24 ± 0.023

6.293 ± 0.048

5.773 ± 0.035

5.327 ± 0.045

6.937 ± 0.033

1.425 ± 0.019

2.396 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski