Capybara microvirus Cap3_SP_433
Average proteome isoelectric point is 5.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W633|A0A4P8W633_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_433 OX=2585451 PE=4 SV=1
MM1 pKa = 7.46 AAASTAAGIGSILSGSGSMLSGSVSNGSSYY31 pKa = 10.02 STSSDD36 pKa = 2.48 RR37 pKa = 11.84 GYY39 pKa = 11.03 SDD41 pKa = 3.93 SSSSSWEE48 pKa = 3.93 GGSSYY53 pKa = 11.26 GSADD57 pKa = 3.51 SYY59 pKa = 11.83 SSANSASAYY68 pKa = 9.71 YY69 pKa = 10.67 DD70 pKa = 3.42 SAQSEE75 pKa = 4.15 NWANSWTEE83 pKa = 3.36 ASAANEE89 pKa = 3.92 QAAKK93 pKa = 9.53 SAYY96 pKa = 9.84 YY97 pKa = 10.57 SNQLQEE103 pKa = 3.77 RR104 pKa = 11.84 WYY106 pKa = 10.39 KK107 pKa = 10.87 EE108 pKa = 3.48 QMQYY112 pKa = 11.04 NAEE115 pKa = 3.97 QAKK118 pKa = 6.4 TQRR121 pKa = 11.84 EE122 pKa = 4.28 YY123 pKa = 11.22 EE124 pKa = 3.86 EE125 pKa = 5.49 RR126 pKa = 11.84 MANTAYY132 pKa = 10.0 QRR134 pKa = 11.84 AVADD138 pKa = 4.63 LKK140 pKa = 11.3 AAGLNPILAAMLGGSATPAASAASVMAPSTSKK172 pKa = 11.31 AEE174 pKa = 4.18 TYY176 pKa = 9.7 MNSGSSAYY184 pKa = 10.17 GYY186 pKa = 9.86 GKK188 pKa = 10.4 SYY190 pKa = 11.42 GEE192 pKa = 4.19 GWSNSNQYY200 pKa = 10.44 SEE202 pKa = 4.52 SHH204 pKa = 6.16 EE205 pKa = 4.23 KK206 pKa = 10.62 SGSSWGGGSQSTSHH220 pKa = 6.04 SQNDD224 pKa = 3.53 AKK226 pKa = 10.9 SVSRR230 pKa = 11.84 SSSTASSNLVNGLSAIGAGIKK251 pKa = 8.82 EE252 pKa = 4.01 LWYY255 pKa = 10.69 KK256 pKa = 10.94 GEE258 pKa = 3.97 EE259 pKa = 4.02 RR260 pKa = 4.48
Molecular weight: 27.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.792
IPC2_protein 5.08
IPC_protein 4.94
Toseland 4.838
ProMoST 5.029
Dawson 4.876
Bjellqvist 5.016
Wikipedia 4.711
Rodwell 4.813
Grimsley 4.762
Solomon 4.876
Lehninger 4.825
Nozaki 4.991
DTASelect 5.067
Thurlkill 4.825
EMBOSS 4.736
Sillero 5.067
Patrickios 2.066
IPC_peptide 4.889
IPC2_peptide 5.067
IPC2.peptide.svr19 5.036
Protein with the highest isoelectric point:
>tr|A0A4P8W4Z4|A0A4P8W4Z4_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_433 OX=2585451 PE=3 SV=1
MM1 pKa = 7.34 SCYY4 pKa = 9.61 HH5 pKa = 6.55 PKK7 pKa = 10.22 IIIITEE13 pKa = 3.81 EE14 pKa = 3.63 AKK16 pKa = 10.79 LNKK19 pKa = 9.6 NGKK22 pKa = 9.2 IGSLVKK28 pKa = 10.29 FVEE31 pKa = 5.01 DD32 pKa = 3.51 EE33 pKa = 4.39 KK34 pKa = 11.64 AWGGYY39 pKa = 7.47 EE40 pKa = 4.36 NIEE43 pKa = 4.19 KK44 pKa = 10.48 FNAKK48 pKa = 10.26 AEE50 pKa = 4.22 EE51 pKa = 3.91 QRR53 pKa = 11.84 KK54 pKa = 9.86 KK55 pKa = 11.02 YY56 pKa = 10.31 GLKK59 pKa = 8.81 KK60 pKa = 9.07 TYY62 pKa = 9.79 ATMAPCGKK70 pKa = 10.01 CQGCRR75 pKa = 11.84 FKK77 pKa = 10.84 QSKK80 pKa = 8.2 EE81 pKa = 3.41 WAVRR85 pKa = 11.84 IEE87 pKa = 4.48 KK88 pKa = 9.66 EE89 pKa = 3.8 AKK91 pKa = 9.31 QYY93 pKa = 8.79 KK94 pKa = 9.55 HH95 pKa = 6.47 NYY97 pKa = 8.74 FVTLTYY103 pKa = 10.83 NDD105 pKa = 3.43 EE106 pKa = 4.12 NLPIKK111 pKa = 10.31 EE112 pKa = 4.9 GYY114 pKa = 9.12 PEE116 pKa = 4.36 SKK118 pKa = 9.36 GTLKK122 pKa = 10.64 KK123 pKa = 10.24 EE124 pKa = 3.67 HH125 pKa = 7.01 TIQFMKK131 pKa = 10.52 SIRR134 pKa = 11.84 QSIKK138 pKa = 9.1 RR139 pKa = 11.84 HH140 pKa = 5.08 YY141 pKa = 9.37 NHH143 pKa = 7.49 DD144 pKa = 3.98 GIKK147 pKa = 10.24 FYY149 pKa = 11.32 LCGEE153 pKa = 4.25 YY154 pKa = 10.47 GSKK157 pKa = 10.83 GEE159 pKa = 4.11 RR160 pKa = 11.84 PHH162 pKa = 6.65 YY163 pKa = 9.65 HH164 pKa = 7.24 IIMMNMPEE172 pKa = 4.15 LEE174 pKa = 4.13 KK175 pKa = 11.27 NKK177 pKa = 10.45 LVGQNTKK184 pKa = 9.61 TKK186 pKa = 10.8 DD187 pKa = 3.27 PYY189 pKa = 9.69 FTNDD193 pKa = 3.67 LFDD196 pKa = 4.22 SAWSKK201 pKa = 11.22 GFVIIGKK208 pKa = 7.96 VNWNTISYY216 pKa = 8.94 VAGYY220 pKa = 8.07 CQKK223 pKa = 11.14 KK224 pKa = 10.28 LFGEE228 pKa = 4.3 VGKK231 pKa = 10.62 KK232 pKa = 9.15 IYY234 pKa = 9.81 EE235 pKa = 4.2 MKK237 pKa = 10.39 GQEE240 pKa = 4.2 PIYY243 pKa = 10.27 ATMSRR248 pKa = 11.84 NPGIGRR254 pKa = 11.84 NYY256 pKa = 9.86 YY257 pKa = 9.88 EE258 pKa = 4.36 EE259 pKa = 4.21 NKK261 pKa = 8.35 EE262 pKa = 3.96 TIYY265 pKa = 10.69 RR266 pKa = 11.84 YY267 pKa = 10.74 DD268 pKa = 3.38 EE269 pKa = 4.56 VITSKK274 pKa = 10.76 GKK276 pKa = 9.06 SVKK279 pKa = 9.79 PPSYY283 pKa = 10.24 FDD285 pKa = 3.53 RR286 pKa = 11.84 LMDD289 pKa = 3.91 IGEE292 pKa = 4.31 EE293 pKa = 3.81 DD294 pKa = 4.12 SIILKK299 pKa = 8.94 EE300 pKa = 4.09 LKK302 pKa = 9.73 EE303 pKa = 3.92 KK304 pKa = 10.69 RR305 pKa = 11.84 RR306 pKa = 11.84 EE307 pKa = 3.92 KK308 pKa = 11.06 SNNALKK314 pKa = 10.36 QKK316 pKa = 10.09 YY317 pKa = 9.17 AQTTLTIKK325 pKa = 10.3 EE326 pKa = 3.86 QLALEE331 pKa = 4.24 EE332 pKa = 4.05 RR333 pKa = 11.84 TAVEE337 pKa = 4.21 KK338 pKa = 10.73 LKK340 pKa = 10.68 KK341 pKa = 9.98 YY342 pKa = 10.26 NRR344 pKa = 11.84 DD345 pKa = 3.55 RR346 pKa = 11.84 IGQQ349 pKa = 3.55
Molecular weight: 40.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.772
IPC2_protein 8.785
IPC_protein 8.639
Toseland 9.399
ProMoST 9.165
Dawson 9.677
Bjellqvist 9.385
Wikipedia 9.838
Rodwell 9.999
Grimsley 9.75
Solomon 9.692
Lehninger 9.648
Nozaki 9.487
DTASelect 9.355
Thurlkill 9.516
EMBOSS 9.823
Sillero 9.619
Patrickios 4.774
IPC_peptide 9.692
IPC2_peptide 7.907
IPC2.peptide.svr19 7.856
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1520
86
549
253.3
28.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.632 ± 1.727
0.855 ± 0.283
4.145 ± 0.646
8.947 ± 1.39
3.421 ± 0.954
6.645 ± 0.796
1.645 ± 0.384
6.579 ± 1.116
7.895 ± 2.042
5.329 ± 0.368
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.895 ± 0.355
5.658 ± 0.331
3.684 ± 0.795
4.276 ± 0.408
4.013 ± 0.324
8.289 ± 3.476
6.513 ± 0.993
3.355 ± 0.604
1.974 ± 0.344
6.25 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here